FastQCFastQC Report
Mon 6 Jun 2016
ERR1211479_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211479_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3085204
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224920.7290279670323259No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194960.6319193155460708No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA187890.6090034889102958No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146360.474393265404816No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137960.4471665406890436No Hit
GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA89170.289024647964932No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG77970.25272234834390206No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64440.20886787389099715No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG38710.12546982306518467No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG37110.12028378026218038No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33990.11017099679632206No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33660.10910137546820242No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC32440.10514701783091167No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG84000.075.5973362
ACCTGGG87300.070.4677053
TATAACG8150.068.104592
TAACGCA8800.065.153064
GTACCTG98550.064.963231
TATCACG3700.061.0227132
GTACCCG7650.057.800621
CCTGGGG45450.057.1806224
TACCCGG7800.054.275012
ATAACGC12100.046.6080133
CTGGGGG32950.045.2120745
CCTGGGA61550.042.6816674
GTATAAC15450.041.4072651
ACCCGGG9750.040.971233
TCACGCA5700.040.3997654
ATCACGC5800.039.7038653
TGGGGGG71150.034.8729366
ATGGGGG57050.032.6204645
CATGGGG93100.030.2872074
CACGCAG7950.030.1476635
Produced by FastQC (version 0.11.5)