##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211479_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3085204
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.102861269465485	33.0	31.0	34.0	30.0	34.0
2	32.28194310651743	33.0	31.0	34.0	30.0	34.0
3	32.27975297581619	34.0	31.0	34.0	30.0	34.0
4	35.94995371456798	37.0	35.0	37.0	35.0	37.0
5	35.90021405391669	37.0	35.0	37.0	35.0	37.0
6	35.9395916769199	37.0	35.0	37.0	35.0	37.0
7	35.88156050620964	37.0	35.0	37.0	35.0	37.0
8	35.87285476098177	37.0	35.0	37.0	35.0	37.0
9	37.5167052162515	39.0	37.0	39.0	35.0	39.0
10-11	37.45949068521887	39.0	37.0	39.0	34.5	39.0
12-13	37.36770972032967	39.0	37.0	39.0	34.0	39.0
14-15	38.54622984412052	40.0	38.0	41.0	34.0	41.0
16-17	38.46021608295594	40.0	38.0	41.0	34.0	41.0
18-19	38.49541262101307	40.0	38.0	41.0	34.0	41.0
20-21	38.436437590512654	40.0	38.0	41.0	34.0	41.0
22-23	38.29205313489805	40.0	37.5	41.0	34.0	41.0
24-25	38.183580080928195	40.0	37.0	41.0	34.0	41.0
26-27	37.97864095858815	40.0	37.0	41.0	33.0	41.0
28-29	37.97484493732019	40.0	37.0	41.0	33.0	41.0
30-31	37.79560281913287	40.0	37.0	41.0	33.0	41.0
32-33	37.42272877255442	39.5	36.0	41.0	32.5	41.0
34-35	37.20557878830702	39.5	36.0	41.0	31.5	41.0
36-37	37.08102608449879	40.0	36.0	41.0	31.0	41.0
38-39	36.949370122688805	40.0	35.0	41.0	31.0	41.0
40-41	36.76754956884537	39.0	35.0	41.0	30.0	41.0
42-43	36.60830240074887	39.0	35.0	41.0	30.0	41.0
44-45	36.382569029470986	39.0	35.0	41.0	29.5	41.0
46-47	36.02214521308802	39.0	35.0	41.0	27.5	41.0
48-49	35.7296899978089	39.0	35.0	41.0	26.0	41.0
50-51	35.51213777111659	39.0	35.0	40.0	25.0	41.0
52-53	35.35083401292103	39.0	34.5	40.0	24.0	41.0
54-55	35.171356902169194	38.0	34.0	40.0	24.0	41.0
56-57	34.975577952057634	38.0	34.0	40.0	23.5	41.0
58-59	34.73988656827879	38.0	34.0	40.0	23.0	41.0
60-61	34.39988538845405	38.0	33.0	40.0	22.5	41.0
62-63	34.014259997069885	37.0	33.0	40.0	22.0	41.0
64-65	33.74668595658504	37.0	32.5	39.5	21.5	41.0
66-67	33.41253836051036	36.0	32.0	39.0	20.5	40.5
68-69	33.019941306960575	36.0	31.5	39.0	20.0	40.0
70-71	32.62867560783663	35.0	31.0	38.5	19.5	40.0
72-73	32.20755029489136	35.0	31.0	37.5	19.0	40.0
74-75	31.786720262258186	35.0	30.5	37.0	18.0	39.0
76-77	30.226181801916503	33.5	28.5	35.5	16.5	38.0
78-79	31.037707068965293	34.0	30.0	36.0	18.0	38.5
80-81	31.024727052084724	34.0	30.0	36.0	18.0	37.5
82-83	30.76030110164514	34.0	30.0	35.0	17.5	37.0
84-85	30.44923512351209	34.0	29.0	35.0	16.0	36.5
86-87	30.15297108392184	34.0	29.0	35.0	15.0	36.0
88-89	29.904292552453583	34.0	29.0	35.0	12.5	36.0
90-91	29.596767345044285	34.0	29.0	35.0	11.5	35.5
92-93	29.34544684889557	34.0	28.5	35.0	10.0	35.0
94-95	29.16740724438319	34.0	27.0	35.0	10.0	35.0
96-97	28.96565705217548	33.5	27.0	35.0	10.0	35.0
98-99	28.767782292516152	33.0	26.5	35.0	10.0	35.0
100	27.031335691254128	31.0	24.0	34.0	8.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3269751182263292
1101	2	0.24099708508489215
1101	3	0.29586395647981334
1101	4	0.19173304555944526
1101	5	0.15575686034921432
1101	6	0.15518284858983833
1101	7	0.16700830486627183
1101	8	0.1777512628693998
1101	9	0.1866715513317132
1101	10-11	0.15654487572023612
1101	12-13	0.14529338523080781
1101	14-15	0.1819454128418343
1101	16-17	0.2569401995282661
1101	18-19	0.2823111066946069
1101	20-21	0.31308386593987336
1101	22-23	0.3285251372525977
1101	24-25	0.2831614095390762
1101	26-27	0.1897679771321421
1101	28-29	0.2696665677455954
1101	30-31	0.40801709666673247
1101	32-33	0.47263317155942985
1101	34-35	0.4990408250892102
1101	36-37	0.5697080932359668
1101	38-39	0.5080648555944691
1101	40-41	0.5121992128615034
1101	42-43	0.503140925229026
1101	44-45	0.4986870881960854
1101	46-47	0.5449993879901953
1101	48-49	0.457152301688879
1101	50-51	0.4304302409449221
1101	52-53	0.47283357231349754
1101	54-55	0.48651460219277
1101	56-57	0.585146770826853
1101	58-59	0.6099377668867803
1101	60-61	0.4669870635493467
1101	62-63	0.4518698517030373
1101	64-65	0.4134288446928025
1101	66-67	0.3055845377341271
1101	68-69	0.2586778393473992
1101	70-71	0.42403819032678314
1101	72-73	0.2865283540432628
1101	74-75	0.3039419361658453
1101	76-77	0.2893800998045677
1101	78-79	0.2768306701422354
1101	80-81	0.3226931094113539
1101	82-83	0.252503212549243
1101	84-85	0.316093528984009
1101	86-87	0.25642448837962206
1101	88-89	0.29914221750627945
1101	90-91	0.07135896911981021
1101	92-93	0.05647989532812758
1101	94-95	0.15066334829496952
1101	96-97	0.06084989184815015
1101	98-99	-0.05537058923822258
1101	100	0.049262513807811104
1102	1	0.2802766483195853
1102	2	0.2408760381516899
1102	3	0.28757992635358676
1102	4	0.19561982529722144
1102	5	0.15297214427521055
1102	6	0.20176827466640646
1102	7	0.1910684518232486
1102	8	0.18135056750887912
1102	9	0.2070391756878891
1102	10-11	0.18242113592475562
1102	12-13	0.19102243381191641
1102	14-15	0.25659658440972066
1102	16-17	0.2858343116544688
1102	18-19	0.2597349896819665
1102	20-21	0.3120443719906305
1102	22-23	0.3297115518033351
1102	24-25	0.3014247634667555
1102	26-27	0.2611191873968437
1102	28-29	0.35448013409654777
1102	30-31	0.38356661654876234
1102	32-33	0.49727986411795655
1102	34-35	0.5474068051714909
1102	36-37	0.5453604257085161
1102	38-39	0.522966678892324
1102	40-41	0.554565457591849
1102	42-43	0.4523885653367543
1102	44-45	0.46102630426485547
1102	46-47	0.3662295285856203
1102	48-49	0.49453620553887134
1102	50-51	0.46220506090921276
1102	52-53	0.5136734070312272
1102	54-55	0.505340901662521
1102	56-57	0.32776333988748974
1102	58-59	0.3741523919209442
1102	60-61	0.30085915122698026
1102	62-63	0.47842692133048814
1102	64-65	0.5171294613409856
1102	66-67	0.5270094356604531
1102	68-69	0.3666218285395786
1102	70-71	0.5668771638015002
1102	72-73	0.5504557768121856
1102	74-75	0.3598350620005384
1102	76-77	0.33332873079649517
1102	78-79	0.4253269734816705
1102	80-81	0.5410033906249332
1102	82-83	0.5130883394188857
1102	84-85	0.3304841995485539
1102	86-87	0.2649876267237161
1102	88-89	0.2765331786380294
1102	90-91	0.33172837598528915
1102	92-93	0.3302389937421921
1102	94-95	0.34362193632933113
1102	96-97	0.3294123844873411
1102	98-99	0.3790239282724279
1102	100	0.3838710688521054
1103	1	0.29397624962481217
1103	2	0.2914988269550065
1103	3	0.37184781871693673
1103	4	0.18512696788560135
1103	5	0.2172383162938516
1103	6	0.1910627314115203
1103	7	0.20187015291860178
1103	8	0.23532802850246526
1103	9	0.3105983233896197
1103	10-11	0.2645338384288536
1103	12-13	0.25576717121606407
1103	14-15	0.36135525891881315
1103	16-17	0.3827530088215809
1103	18-19	0.3765536898112032
1103	20-21	0.3356171419048408
1103	22-23	0.36060957441208075
1103	24-25	0.3289038455783526
1103	26-27	0.26592721284940524
1103	28-29	0.43320244991181056
1103	30-31	0.45355693760831883
1103	32-33	0.589504306302409
1103	34-35	0.6246913344423888
1103	36-37	0.6465685542394368
1103	38-39	0.5699551282986661
1103	40-41	0.6522283328286989
1103	42-43	0.6928671823148136
1103	44-45	0.7070595264874058
1103	46-47	0.7536573846313743
1103	48-49	0.7069685781566406
1103	50-51	0.7027256635493515
1103	52-53	0.6591405166466373
1103	54-55	0.7304275037620016
1103	56-57	0.8228141020651378
1103	58-59	0.8105096447172357
1103	60-61	0.7364137395704589
1103	62-63	0.7548817356404456
1103	64-65	0.7838838531346397
1103	66-67	0.8121809893425436
1103	68-69	0.9523956451954803
1103	70-71	0.9079719737421001
1103	72-73	0.8008198823445696
1103	74-75	0.7203746595946576
1103	76-77	0.7790363328610113
1103	78-79	0.813265506828202
1103	80-81	0.7811605771159016
1103	82-83	0.6701972191818228
1103	84-85	0.664945452786764
1103	86-87	0.7266446655977248
1103	88-89	0.6909162744988535
1103	90-91	0.7907679824302001
1103	92-93	0.6772921651140571
1103	94-95	0.7298424822087632
1103	96-97	0.8160564963140153
1103	98-99	0.8545013661827952
1103	100	0.8971728293233703
1104	1	0.3010178465723996
1104	2	0.23522132044102761
1104	3	0.306653098554996
1104	4	0.09640016235290005
1104	5	0.12631116622120686
1104	6	0.13630018003600952
1104	7	0.14014864332791888
1104	8	0.14279004117232574
1104	9	0.21932322540622096
1104	10-11	0.20766422009972274
1104	12-13	0.2855774164412068
1104	14-15	0.32008043463206803
1104	16-17	0.295602916753559
1104	18-19	0.34852388548135593
1104	20-21	0.17774343649002589
1104	22-23	0.27334801717346124
1104	24-25	0.2638082742374621
1104	26-27	0.35542372401163647
1104	28-29	0.39082506954549956
1104	30-31	0.3927169078301276
1104	32-33	0.45945892916254394
1104	34-35	0.5565294335114004
1104	36-37	0.35252811352778224
1104	38-39	0.4162700411995601
1104	40-41	0.5677124386458274
1104	42-43	0.6776555194469367
1104	44-45	0.5629095453106743
1104	46-47	0.5298515231437761
1104	48-49	0.6050312296168343
1104	50-51	0.5474037821549942
1104	52-53	0.5952311112994124
1104	54-55	0.55820653132308
1104	56-57	0.6293566087451055
1104	58-59	0.746124801790863
1104	60-61	0.5236593959162334
1104	62-63	0.47523720932636593
1104	64-65	0.8482582779302135
1104	66-67	0.8041537754191168
1104	68-69	0.8115986687842565
1104	70-71	0.6363820751617197
1104	72-73	0.6204140555369833
1104	74-75	0.7267146660644919
1104	76-77	0.682248719347534
1104	78-79	0.8868086813280094
1104	80-81	0.7734953490930359
1104	82-83	0.5979473938179467
1104	84-85	0.7282525489111471
1104	86-87	0.8030589180244405
1104	88-89	0.699793757986999
1104	90-91	0.561808412900298
1104	92-93	0.4973827004364644
1104	94-95	0.6718615671649104
1104	96-97	0.5814190013553286
1104	98-99	0.7505097035126873
1104	100	0.8175339005473639
1105	1	0.2053393863518309
1105	2	0.11970550334946495
1105	3	0.19535570581903272
1105	4	0.1512724024882175
1105	5	0.0924790538384812
1105	6	0.10798652444152879
1105	7	0.134100065637206
1105	8	0.1732027282962747
1105	9	0.21285360518803031
1105	10-11	0.20134049220809658
1105	12-13	0.17222080578265064
1105	14-15	0.22349598760852274
1105	16-17	0.20303728065938031
1105	18-19	0.18965068691072418
1105	20-21	0.07670559211141637
1105	22-23	0.2380829785378964
1105	24-25	0.24132813013765997
1105	26-27	0.21712288876216235
1105	28-29	0.3629229121206805
1105	30-31	0.36186972517911187
1105	32-33	0.3642805932913191
1105	34-35	0.41899660092052216
1105	36-37	0.4679908458101778
1105	38-39	0.41082921185486043
1105	40-41	0.4137294963221123
1105	42-43	0.4752494141121346
1105	44-45	0.513822549489106
1105	46-47	0.5997975766154511
1105	48-49	0.6516407828463642
1105	50-51	0.6695313849468221
1105	52-53	0.641465434918949
1105	54-55	0.6291160195418826
1105	56-57	0.7018056473283067
1105	58-59	0.8056475984440468
1105	60-61	0.7538793960907597
1105	62-63	0.6921519202177748
1105	64-65	0.7210603486740865
1105	66-67	0.565194052245495
1105	68-69	0.6392871962116473
1105	70-71	0.695315666792645
1105	72-73	0.7354648351564919
1105	74-75	0.7704673657542749
1105	76-77	0.7346256098575665
1105	78-79	0.8291673048461767
1105	80-81	0.7175836829426778
1105	82-83	0.6763407493450977
1105	84-85	0.6472498568568525
1105	86-87	0.7568502278230227
1105	88-89	0.6915590678641195
1105	90-91	0.6299883056835291
1105	92-93	0.5577288570235588
1105	94-95	0.6531994388858351
1105	96-97	0.6862353206437319
1105	98-99	0.6595856060635228
1105	100	0.8030215681462884
1106	1	0.19877717121391214
1106	2	0.1570349218488971
1106	3	0.22934917773837782
1106	4	0.15540015723920675
1106	5	0.11081794661646427
1106	6	0.13114621396319137
1106	7	0.13261717400973083
1106	8	0.15441812865743287
1106	9	0.07382132347667181
1106	10-11	0.11925138888748421
1106	12-13	0.18083696572006858
1106	14-15	0.23409432598648294
1106	16-17	0.2328846715255537
1106	18-19	0.24008732854729686
1106	20-21	0.236692111787157
1106	22-23	0.3263547606770558
1106	24-25	0.22506024940243208
1106	26-27	0.31181978851141423
1106	28-29	0.322681010527603
1106	30-31	0.31221300060132506
1106	32-33	0.5063590981336645
1106	34-35	0.4039756082113115
1106	36-37	0.4557055083628043
1106	38-39	0.5436134835788025
1106	40-41	0.5616163197947728
1106	42-43	0.48938654745318644
1106	44-45	0.6239799934092218
1106	46-47	0.6552083403941751
1106	48-49	0.5251421529822622
1106	50-51	0.5597236796180951
1106	52-53	0.6986702177760549
1106	54-55	0.5880793719737483
1106	56-57	0.653334956261638
1106	58-59	0.7041359722938978
1106	60-61	0.815525998953909
1106	62-63	0.8046468769872845
1106	64-65	0.8234456218255275
1106	66-67	0.8335381211546249
1106	68-69	0.8374973460172725
1106	70-71	0.8737479593598891
1106	72-73	0.8573427187511768
1106	74-75	0.891646191852498
1106	76-77	0.7466769022395923
1106	78-79	0.7169927086263748
1106	80-81	0.856598337829027
1106	82-83	0.8653154221156818
1106	84-85	0.8800199152044428
1106	86-87	0.7529656113407697
1106	88-89	0.9808959858781172
1106	90-91	0.9896261779522781
1106	92-93	0.7435118154091462
1106	94-95	0.8936304511300435
1106	96-97	0.9273484630365836
1106	98-99	1.052140314971897
1106	100	1.0850860218909801
1107	1	0.1515527185876948
1107	2	0.17352087139172312
1107	3	0.2192828930329327
1107	4	0.08631139876548843
1107	5	0.10804308831058052
1107	6	0.0599554357226495
1107	7	0.09086138185001147
1107	8	0.04051538587705039
1107	9	0.08650162353310265
1107	10-11	0.08478044978222243
1107	12-13	0.1809391019030926
1107	14-15	0.18103491890402523
1107	16-17	0.15394419139754234
1107	18-19	0.06186648917555004
1107	20-21	0.16672257040173832
1107	22-23	0.2190054163070272
1107	24-25	0.21191133783958094
1107	26-27	0.2821361486516665
1107	28-29	0.28812448193563966
1107	30-31	0.1698571028328928
1107	32-33	0.16504037449050912
1107	34-35	0.3558827940289859
1107	36-37	0.45024405590920935
1107	38-39	0.45207557287620403
1107	40-41	0.37430912280694173
1107	42-43	0.4475739525250191
1107	44-45	0.5194874248252077
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2316	72-73	-0.6190328518886261
2316	74-75	-0.7359614076470713
2316	76-77	-0.608012129241871
2316	78-79	-0.5381336629833591
2316	80-81	-0.41024204614904036
2316	82-83	-0.32251653802589786
2316	84-85	-0.4756941702882891
2316	86-87	-0.45261884241297423
2316	88-89	-0.5064708843649868
2316	90-91	-0.3708094934398183
2316	92-93	-0.2723640531188174
2316	94-95	-0.2832212249420145
2316	96-97	-0.3110044381034527
2316	98-99	-0.4060071253696158
2316	100	-0.5485337621951842
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	8.0
11	17.0
12	54.0
13	183.0
14	593.0
15	1787.0
16	4509.0
17	10344.0
18	19222.0
19	26035.0
20	29698.0
21	35186.0
22	41648.0
23	42274.0
24	40230.0
25	39121.0
26	42435.0
27	48256.0
28	57194.0
29	67631.0
30	81192.0
31	97101.0
32	119152.0
33	150351.0
34	203133.0
35	266666.0
36	376651.0
37	591211.0
38	598045.0
39	95249.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.68690056701511	18.37546428757808	12.380785989965327	21.556849155441487
2	15.099306558582484	21.80547117785788	42.539560931797915	20.55566133176172
3	17.088069701183404	32.80623109909664	30.195289076452838	19.910410123267113
4	10.869224176938326	17.838621179649344	37.94572648409108	33.34642815932124
5	10.298799473347453	38.31805690881404	34.25468357089097	17.128460046947534
6	25.32292965883485	36.290059095187594	23.75848893683116	14.6285223091464
7	25.908367809713717	33.27407199005317	24.605536619296487	16.212023580936624
8	27.911831171811492	33.54380242009197	22.52931116968519	16.015055238411342
9	29.92663041188227	15.120760326228233	21.89328283853051	33.05932642335899
10-11	23.89751212561633	26.932870565447214	30.21649459808817	18.953122710848294
12-13	25.074824873817096	25.02541160973472	29.77704229606859	20.122721220379592
14-15	23.021702940875223	25.566072778331677	29.232361944299306	22.179862336493795
16-17	20.858536477849512	27.39988794820919	30.62332981814192	21.11824575579938
18-19	20.670451541719924	26.223314110757755	32.92528510137757	20.180949246144746
20-21	21.44827095528037	26.58718138588399	32.707859179258236	19.256688479577402
22-23	20.567618867342322	26.68998549204526	31.838607754949106	20.903787885663313
24-25	20.14199387495833	26.649716947358577	32.078483639733975	21.129805537949117
26-27	20.314384753924422	25.908688916652256	32.43487740910194	21.342048920321385
28-29	20.463118350756723	26.903179643925906	32.5023479238195	20.131354081497875
30-31	22.18564200867834	26.466985591962537	32.49788584229048	18.849486557068644
32-33	20.872888186159702	26.496812354938527	32.94961986013809	19.680679598763675
34-35	20.668404385820327	27.36784493490198	32.233345115853616	19.730405563424075
36-37	21.374039615792835	27.07987288173363	31.735160446375772	19.810927056097764
38-39	21.704724445818048	27.628153589016826	30.983997403048242	19.683124562116884
40-41	21.145325470409173	27.38424979748962	31.701651233522433	19.76877349857878
42-43	21.22725260790409	27.256578735178465	30.720861433716372	20.795307223201064
44-45	20.689502041790085	27.645704850313024	31.265076799702307	20.39971630819458
46-47	20.44268999431277	26.753977861448398	31.496665862231545	21.306666282007296
48-49	20.577743597307236	27.248797177896112	31.422713462936386	20.750745761860266
50-51	20.46780933907133	27.809494666333002	31.470418148601002	20.25227784599466
52-53	20.27133511697273	28.377937283919564	29.750382324207038	21.60034527490067
54-55	20.20178102907612	28.123157996924924	30.424522300787345	21.250538673211615
56-57	21.06524133158408	27.79414755816017	29.613427019510567	21.52718409074518
58-59	20.681266153200124	28.085511417183547	30.448021520150746	20.78520090946558
60-61	21.89590433978004	28.512407209103653	29.990902638785812	19.6007858123305
62-63	20.83507508566666	28.32334854729346	30.707103091585402	20.13447327545448
64-65	20.3780762515415	27.8386147717123	30.211044141526333	21.57226483521987
66-67	20.86544485009583	27.62155832262927	29.993427637150464	21.51956919012444
68-69	20.716537639710317	27.890909697987286	30.19600330067827	21.196549361624122
70-71	20.375707975516598	28.028569585569535	30.486455718892756	21.109266720021107
72-73	20.414540153755613	28.559539092964076	29.310342871488952	21.715577881791358
74-75	20.98289589862008	28.15638869044455	29.368599089561858	21.492116321373516
76-77	20.200177367837945	27.61055183049962	30.71247934436134	21.476791457301093
78-79	20.230703751630497	27.724078040760443	30.477248916676825	21.56796929093224
80-81	19.864409197030486	28.45790988219678	29.67146863834646	22.006212282426276
82-83	20.40611133155423	28.736364944876254	28.950065095555605	21.907458628013913
84-85	20.10977885378467	29.25019840137766	28.363989371910513	22.27603337292716
86-87	19.915137470424877	28.518118552071993	29.27763754336982	22.289106434133306
88-89	19.727170871253282	28.14632389365383	29.717937811713018	22.40856742337987
90-91	21.130706283738668	28.176620132882192	29.334844996444275	21.35782858693487
92-93	19.898824783259077	28.629580651682655	29.474139508899437	21.997455056158827
94-95	19.43025938421581	28.27161494600418	29.210871471951517	23.087254197828493
96-97	20.011489676797726	27.990736285981427	29.328842719811394	22.668931317409456
98-99	20.250602256529024	27.64275611260795	29.113247568848188	22.993394062014836
100	18.423025053166658	29.105406141397378	29.479161263551013	22.99240754188495
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2439.0
1	1955.5
2	5022.5
3	7290.5
4	4460.0
5	3104.0
6	3957.0
7	6630.0
8	9659.5
9	10825.0
10	9589.5
11	8052.0
12	8190.5
13	8740.5
14	9329.5
15	9802.0
16	10140.5
17	10614.5
18	10980.5
19	11282.0
20	11709.5
21	12403.5
22	13730.0
23	15502.5
24	17065.5
25	19299.0
26	23105.5
27	26823.0
28	29811.5
29	34436.0
30	41635.5
31	47760.0
32	55249.0
33	65639.5
34	75665.5
35	83750.0
36	94976.0
37	104656.5
38	106212.5
39	106943.5
40	102518.5
41	96064.0
42	91526.0
43	88310.5
44	89282.5
45	92341.5
46	93241.0
47	92179.0
48	96109.5
49	109169.0
50	139879.0
51	143793.0
52	112389.5
53	93092.0
54	84029.0
55	75131.5
56	66340.5
57	60084.0
58	53125.0
59	43524.0
60	32137.0
61	22926.0
62	17639.5
63	13526.5
64	9919.5
65	7268.0
66	5394.5
67	4149.0
68	3160.5
69	2373.0
70	1829.5
71	1382.5
72	1041.0
73	829.5
74	670.5
75	494.5
76	354.5
77	231.5
78	155.0
79	109.0
80	67.5
81	53.5
82	34.0
83	22.0
84	19.0
85	15.5
86	10.0
87	6.0
88	9.0
89	8.0
90	3.0
91	2.5
92	2.5
93	2.0
94	1.5
95	3.0
96	2.5
97	0.5
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0032736895193964484
2	0.07049776935333936
3	0.0011020340956384084
4	5.186042803004275E-4
5	0.0018799405160890496
6	3.889532102253206E-4
7	0.0
8	0.001037208560600855
9	6.482553503755344E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	7.617000366912528E-4
18-19	9.561766418039131E-4
20-21	0.003387134205712167
22-23	0.0
24-25	1.620638375938836E-5
26-27	1.1344468631571851E-4
28-29	0.0010210021768414666
30-31	0.0016530511434576125
32-33	0.002900942692930516
34-35	0.004067802323606478
36-37	0.0014747809221043406
38-39	0.0015720192246606707
40-41	0.005737059850823479
42-43	0.006725649260146169
44-45	0.005380519408116935
46-47	0.0072442535404465954
48-49	0.008800066381347877
50-51	0.008816272765107267
52-53	0.0040840087073658665
54-55	0.0025119894827051953
56-57	0.0026416405527803027
58-59	0.003095419298043177
60-61	0.00789250889082213
62-63	0.013856458114277046
64-65	0.0044891683013505755
66-67	0.01025864091969283
68-69	0.01243029634345087
70-71	0.005672234315785925
72-73	0.00789250889082213
74-75	0.0031116256818025647
76-77	0.0029171490766899044
78-79	0.008168017414731733
80-81	0.0060449811422518575
82-83	0.016465685899538573
84-85	0.01776219660028964
86-87	0.022235158517880826
88-89	0.02061452014194199
90-91	0.024066479882691713
92-93	0.034017199510956164
94-95	0.03789052522944998
96-97	0.022559286193068594
98-99	0.017989085972921075
100	0.01828080088059007
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3085204.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.45420212107854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.89989902827313	46.70484847252443
2	13.96411704419385	16.325226402874097
3	3.1763125885367693	5.570064541501634
4	1.033715404094944	2.4170003666655293
5	0.4864620033571355	1.4217874134231392
6	0.2879234149162085	1.0098200094541925
7	0.19762578894441862	0.808644046790626
8	0.13741386347387877	0.642593419979232
9	0.1103325955842483	0.580446345854238
>10	0.5811986505778812	6.260304352387042
>50	0.05887325193650228	2.4367695183073175
>100	0.057485930845245774	6.718815641811878
>500	0.005360853997038514	2.1668598243188177
>1k	0.0027750303043351625	2.7942523057674986
>5k	1.8920661165921563E-4	0.8537522085780369
>10k+	3.1534435276535936E-4	3.2888151297624195
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22492	0.7290279670323259	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19496	0.6319193155460708	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	18789	0.6090034889102958	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14636	0.474393265404816	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13796	0.4471665406890436	No Hit
GTACCTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	8917	0.289024647964932	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	7797	0.25272234834390206	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6444	0.20886787389099715	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	3871	0.12546982306518467	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	3711	0.12028378026218038	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3399	0.11017099679632206	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3366	0.10910137546820242	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	3244	0.10514701783091167	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.241276751877672E-5	0.0
10-11	0.0	0.0	0.0	3.241276751877672E-5	0.0
12-13	0.0	0.0	0.0	3.241276751877672E-5	0.0
14-15	0.0	0.0	0.0	6.482553503755344E-5	0.0
16-17	0.0	0.0	0.0	8.103191879694179E-5	0.0
18-19	0.0	0.0	0.0	1.1344468631571851E-4	0.0
20-21	0.0	0.0	0.0	1.458574538344952E-4	0.0
22-23	0.0	0.0	0.0	4.699851290222624E-4	0.0
24-25	1.620638375938836E-5	0.0	0.0	9.723830255633015E-4	0.0
26-27	3.241276751877672E-5	0.0	0.0	0.001539606457141894	0.0
28-29	3.241276751877672E-5	0.0	0.0	0.0035978171945842156	0.0
30-31	6.482553503755344E-5	0.0	0.0	0.009966926012023841	3.241276751877672E-5
32-33	6.482553503755344E-5	0.0	0.0	0.024309575639082537	9.723830255633015E-5
34-35	6.482553503755344E-5	0.0	0.0	0.04404895105801755	1.2965107007510687E-4
36-37	6.482553503755344E-5	0.0	0.0	0.07372283972145764	1.2965107007510687E-4
38-39	6.482553503755344E-5	0.0	0.0	0.12926211686488154	1.2965107007510687E-4
40-41	6.482553503755344E-5	0.0	0.0	0.21847825946031446	1.2965107007510687E-4
42-43	6.482553503755344E-5	0.0	0.0	0.29182835235530613	1.2965107007510687E-4
44-45	6.482553503755344E-5	0.0	0.0	0.36978105823796414	1.2965107007510687E-4
46-47	6.482553503755344E-5	0.0	0.0	0.45457285806708403	1.2965107007510687E-4
48-49	6.482553503755344E-5	0.0	0.0	0.5503363797013099	1.6206383759388357E-4
50-51	6.482553503755344E-5	0.0	0.0	0.6637162404819907	1.6206383759388357E-4
52-53	6.482553503755344E-5	0.0	0.0	0.7900125891189043	1.6206383759388357E-4
54-55	6.482553503755344E-5	0.0	0.0	0.9489161818797072	1.6206383759388357E-4
56-57	6.482553503755344E-5	0.0	0.0	1.1094242066326894	1.6206383759388357E-4
58-59	6.482553503755344E-5	0.0	0.0	1.284258674628971	1.944766051126603E-4
60-61	6.482553503755344E-5	0.0	0.0	1.4608758448387853	2.2688937263143702E-4
62-63	6.482553503755344E-5	0.0	0.0	1.6652221376609133	2.2688937263143702E-4
64-65	6.482553503755344E-5	0.0	0.0	1.8676398708156738	2.2688937263143702E-4
66-67	6.482553503755344E-5	0.0	0.0	2.0902669645183916	2.2688937263143702E-4
68-69	6.482553503755344E-5	0.0	0.0	2.377962689015054	2.2688937263143702E-4
70-71	6.482553503755344E-5	0.0	0.0	2.7262378760043093	2.2688937263143702E-4
72-73	6.482553503755344E-5	0.0	0.0	3.014565649467588	3.2412767518776714E-4
74-75	6.482553503755344E-5	0.0	0.0	3.306977431638232	3.2412767518776714E-4
76-77	8.103191879694179E-5	0.0	0.0	3.6231477724001397	3.2412767518776714E-4
78-79	9.723830255633015E-5	0.0	0.0	3.991843002926225	3.403340589471555E-4
80-81	9.723830255633015E-5	0.0	0.0	4.346082139138936	3.7274682646593223E-4
82-83	9.723830255633015E-5	0.0	0.0	4.737369068625608	3.889532102253206E-4
84-85	9.723830255633015E-5	0.0	0.0	5.148038833088509	3.889532102253206E-4
86-87	9.723830255633015E-5	0.0	0.0	5.563359829690354	3.889532102253206E-4
88	9.723830255633015E-5	0.0	0.0	5.895136918012553	3.889532102253206E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	8400	0.0	75.597336	2
ACCTGGG	8730	0.0	70.467705	3
TATAACG	815	0.0	68.10459	2
TAACGCA	880	0.0	65.15306	4
GTACCTG	9855	0.0	64.96323	1
TATCACG	370	0.0	61.022713	2
GTACCCG	765	0.0	57.80062	1
CCTGGGG	4545	0.0	57.180622	4
TACCCGG	780	0.0	54.27501	2
ATAACGC	1210	0.0	46.608013	3
CTGGGGG	3295	0.0	45.212074	5
CCTGGGA	6155	0.0	42.681667	4
GTATAAC	1545	0.0	41.407265	1
ACCCGGG	975	0.0	40.97123	3
TCACGCA	570	0.0	40.399765	4
ATCACGC	580	0.0	39.703865	3
TGGGGGG	7115	0.0	34.872936	6
ATGGGGG	5705	0.0	32.620464	5
CATGGGG	9310	0.0	30.287207	4
CACGCAG	795	0.0	30.147663	5
>>END_MODULE