FastQCFastQC Report
Mon 6 Jun 2016
ERR1211478_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211478_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871039
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12750.14637691308885134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10440.1198568605998124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG10450.044.5660362
GTACCTG11000.044.516961
TATCACG500.001706844137.5773547
ACCTGGG12800.035.9750943
GGTATCA11750.032.8594931
GTATCAA18750.031.1390421
CATGGGG16050.028.1032874
GTACATG27100.027.9731541
CAACGCA20800.027.1068365
CCTGGGG12150.027.069654
TCAACGC20700.027.0108054
ATTACCG700.00885094126.8471329
ATCAACG21050.026.5632213
TACATGG29050.026.557232
ACATGGG29150.026.4357193
AACGCAG21950.025.891796
TATCAAC22850.024.704732
ATGGGGG10150.024.5341345
TCGACGC802.030134E-623.5128558-59
ACGCAGA24800.022.3494327
Produced by FastQC (version 0.11.5)