##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211478_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 871039 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.20842579953366 31.0 31.0 34.0 28.0 34.0 2 31.25368209689807 31.0 31.0 34.0 28.0 34.0 3 31.36602379457177 33.0 31.0 34.0 28.0 34.0 4 34.85879047895674 37.0 35.0 37.0 32.0 37.0 5 34.935808844380105 37.0 35.0 37.0 32.0 37.0 6 35.05755081000966 37.0 35.0 37.0 32.0 37.0 7 34.97541327081795 37.0 35.0 37.0 32.0 37.0 8 34.93380548976567 37.0 35.0 37.0 32.0 37.0 9 36.50825163970844 39.0 37.0 39.0 32.0 39.0 10-11 36.48955729881211 39.0 37.0 39.0 32.0 39.0 12-13 36.43643453393017 39.0 37.0 39.0 32.0 39.0 14-15 37.636290682736366 40.0 37.5 41.0 32.0 41.0 16-17 37.61488750790723 40.0 37.0 41.0 32.0 41.0 18-19 37.5997670597987 40.0 37.0 41.0 31.5 41.0 20-21 37.533826269547056 40.0 37.0 41.0 31.0 41.0 22-23 37.36648129417856 40.0 37.0 41.0 31.0 41.0 24-25 37.30528598604655 40.0 37.0 41.0 31.0 41.0 26-27 37.17508687900312 40.0 37.0 41.0 30.0 41.0 28-29 37.02104842607507 40.0 36.5 41.0 30.0 41.0 30-31 36.87570935400137 40.0 36.0 41.0 30.0 41.0 32-33 36.75019660428523 39.5 36.0 41.0 30.0 41.0 34-35 36.585655177322714 39.0 36.0 41.0 29.5 41.0 36-37 36.42547980056002 39.0 35.0 41.0 29.0 41.0 38-39 36.310748428026756 39.0 35.0 41.0 28.0 41.0 40-41 36.27286321278382 39.0 35.0 41.0 28.0 41.0 42-43 36.24728513878253 39.0 35.0 41.0 28.0 41.0 44-45 36.0890924516583 39.0 35.0 40.0 27.5 41.0 46-47 36.089462699144356 39.0 35.0 40.0 28.0 41.0 48-49 36.05970283764562 39.0 35.0 41.0 27.0 41.0 50-51 35.117358120589316 38.0 34.0 40.0 25.5 40.5 52-53 35.32398893734953 38.5 34.5 39.5 26.5 40.5 54-55 35.915422271563045 39.0 35.0 40.5 27.0 41.0 56-57 35.893513952876965 39.0 35.0 41.0 27.0 41.0 58-59 35.73383109137478 39.0 34.5 41.0 27.0 41.0 60-61 35.5426151986306 38.0 34.0 40.0 26.0 41.0 62-63 35.29619339662173 38.0 34.0 40.0 26.0 41.0 64-65 35.0239392266018 37.5 34.0 40.0 26.0 41.0 66-67 34.73284606085376 37.0 34.0 40.0 26.0 41.0 68-69 34.44687551303673 36.5 33.5 39.0 26.0 41.0 70-71 34.08723375187563 36.0 33.0 39.0 25.5 41.0 72-73 33.75749421093659 35.5 33.0 39.0 25.0 40.0 74-75 33.37993706366764 35.0 32.5 37.5 25.0 40.0 76-77 33.02058633425139 35.0 32.0 37.0 24.0 39.0 78-79 32.70574968514613 35.0 32.0 37.0 24.0 39.0 80-81 32.35555239202837 35.0 32.0 36.0 23.5 38.5 82-83 32.05043689203354 35.0 31.5 36.0 23.0 37.0 84-85 31.80044636348085 35.0 31.0 35.0 23.0 37.0 86-87 31.49499390957236 34.5 31.0 35.0 21.0 36.5 88-89 31.29131933242943 34.0 31.0 35.0 20.5 36.0 90-91 31.071884266950157 34.0 31.0 35.0 20.0 36.0 92-93 30.93444954818326 34.0 30.5 35.0 20.0 35.5 94-95 30.795615351321814 34.0 30.0 35.0 20.0 35.0 96-97 30.715109197177163 34.0 30.0 35.0 20.0 35.0 98-99 30.549710747739194 34.0 30.0 35.0 19.0 35.0 100 29.201089733065913 33.0 27.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5640144593647634 1101 2 0.37457875297312526 1101 3 0.44001075067765427 1101 4 0.3529132125576879 1101 5 0.4317105640690855 1101 6 0.4025221184570995 1101 7 0.46609999113580614 1101 8 0.46218347584026986 1101 9 0.5878055007236469 1101 10-11 0.4903291094637652 1101 12-13 0.4597384293257676 1101 14-15 0.5696235564550349 1101 16-17 0.6363079993074194 1101 18-19 0.5255863444423596 1101 20-21 0.4919546148447367 1101 22-23 0.5900858341264978 1101 24-25 0.3825962461920014 1101 26-27 0.38947993818922555 1101 28-29 0.46329440811778255 1101 30-31 0.5207353706004199 1101 32-33 0.49086677686125313 1101 34-35 0.4425174932901186 1101 36-37 0.38829857840539006 1101 38-39 0.30326457376548177 1101 40-41 0.29812713320527706 1101 42-43 0.2833345899297868 1101 44-45 0.24098336691493927 1101 46-47 0.07087295631185242 1101 48-49 0.47818553302871436 1101 50-51 0.2871587245358498 1101 52-53 0.46054814099330343 1101 54-55 0.5440311556439354 1101 56-57 0.6226174050480253 1101 58-59 0.7050265115014795 1101 60-61 0.537902500919536 1101 62-63 0.6634078878937046 1101 64-65 0.5333626481605833 1101 66-67 0.6582645273152536 1101 68-69 0.4731907329342988 1101 70-71 0.5416530469744103 1101 72-73 0.5044278961019799 1101 74-75 0.42558638069785815 1101 76-77 0.580087503581403 1101 78-79 0.5646101050197743 1101 80-81 0.32118901869871763 1101 82-83 0.4003133505586902 1101 84-85 0.28330044350116523 1101 86-87 0.31351126812282004 1101 88-89 0.04238042954539978 1101 90-91 -0.03576038272486315 1101 92-93 0.05306124109428367 1101 94-95 0.05206701374447675 1101 96-97 0.06614137759968486 1101 98-99 0.010252639447653422 1101 100 0.22999502252873327 1102 1 0.41547813039650094 1102 2 0.39947052078087 1102 3 0.3709450625408479 1102 4 0.11784873143728447 1102 5 0.34920929094169395 1102 6 0.4412317367545242 1102 7 0.3577107034718594 1102 8 0.40171614317633697 1102 9 0.539026066607498 1102 10-11 0.3678634184468734 1102 12-13 0.47165673447575074 1102 14-15 0.42234988184938516 1102 16-17 0.31079452641226624 1102 18-19 0.4787490023396117 1102 20-21 0.47094244911431105 1102 22-23 0.6213563301683465 1102 24-25 0.5395587802457058 1102 26-27 0.3865854552491328 1102 28-29 0.2101646093439129 1102 30-31 0.34670769300733184 1102 32-33 0.25549923251125506 1102 34-35 0.4759775066170491 1102 36-37 0.4822521069646868 1102 38-39 0.504449465899846 1102 40-41 0.37038644012650934 1102 42-43 0.23879411364268321 1102 44-45 0.29672670966963466 1102 46-47 0.2752344485611573 1102 48-49 0.4761956578279225 1102 50-51 0.23129166914531396 1102 52-53 0.32930016196030465 1102 54-55 0.461635267354481 1102 56-57 0.576704962175107 1102 58-59 0.5260248004706369 1102 60-61 0.2944916715474122 1102 62-63 0.3858654623143778 1102 64-65 0.4258452400254029 1102 66-67 0.22872836460629742 1102 68-69 0.09546856402269555 1102 70-71 0.012682035005049386 1102 72-73 0.280175506615322 1102 74-75 0.0011584370898773955 1102 76-77 0.13820756898545739 1102 78-79 -0.10851231026979491 1102 80-81 -0.07528641485188103 1102 82-83 0.022178151630079412 1102 84-85 0.13403820282314527 1102 86-87 0.09232551196370054 1102 88-89 0.014233513917421448 1102 90-91 0.10167781004953369 1102 92-93 -0.05602843027520876 1102 94-95 0.04178642824988188 1102 96-97 -0.06860982908943924 1102 98-99 -0.29358297591470617 1102 100 0.12858077109491006 1103 1 0.49479941428719343 1103 2 0.5310554557520639 1103 3 0.4482028946349743 1103 4 0.48365375224179274 1103 5 0.3880238914977028 1103 6 0.3655600688107299 1103 7 0.46674673934296607 1103 8 0.4195993920798813 1103 9 0.37016823555947553 1103 10-11 0.4664429319534591 1103 12-13 0.4877602888956929 1103 14-15 0.5904617057681563 1103 16-17 0.533521787251928 1103 18-19 0.4872976817566155 1103 20-21 0.4873611441445078 1103 22-23 0.4722542847619451 1103 24-25 0.6659648173459871 1103 26-27 0.556245521834029 1103 28-29 0.5720597943615218 1103 30-31 0.5168587774310183 1103 32-33 0.48944406067462864 1103 34-35 0.6395289451489319 1103 36-37 0.5012762694883222 1103 38-39 0.41588187804622834 1103 40-41 0.2672923085415775 1103 42-43 0.4063024629073979 1103 44-45 0.17624296229471526 1103 46-47 0.0376537346434489 1103 48-49 0.44054946266042805 1103 50-51 0.5344366678246004 1103 52-53 0.5781102119382027 1103 54-55 0.623238979998554 1103 56-57 0.5030254797902884 1103 58-59 0.3548766425394234 1103 60-61 0.5269895989421585 1103 62-63 0.5649339798981785 1103 64-65 0.41826159699994747 1103 66-67 0.5313901912711145 1103 68-69 0.5951527695937529 1103 70-71 0.6172479124689616 1103 72-73 0.3526290789499029 1103 74-75 0.34779153234602944 1103 76-77 0.2878280926157757 1103 78-79 0.49594830238446974 1103 80-81 0.4024020222337512 1103 82-83 0.35254489161161473 1103 84-85 0.26953951176816204 1103 86-87 0.38890094041108725 1103 88-89 0.12913364965017848 1103 90-91 0.1077079798593239 1103 92-93 0.1606954673210943 1103 94-95 0.15361492376076313 1103 96-97 0.03849678781726951 1103 98-99 0.14799767000585717 1103 100 0.22037691565683915 1104 1 0.39278115244401945 1104 2 0.34269158949207323 1104 3 0.2714843316451301 1104 4 0.26810705520306755 1104 5 0.24748153792062055 1104 6 0.2061984611507981 1104 7 0.25428390007034807 1104 8 0.33183251049229767 1104 9 0.2544053306523324 1104 10-11 0.5203838994836687 1104 12-13 0.3431218096864015 1104 14-15 0.19231950083399596 1104 16-17 0.41967483153592866 1104 18-19 0.29638203178171096 1104 20-21 0.2639720858069907 1104 22-23 0.2573965090003867 1104 24-25 0.20668477024356235 1104 26-27 0.39175983595316666 1104 28-29 0.3941552250398743 1104 30-31 0.3639086017621267 1104 32-33 0.49860755822298586 1104 34-35 0.41274595414473225 1104 36-37 0.3448935526047947 1104 38-39 0.34015603734510336 1104 40-41 0.28341379033764724 1104 42-43 0.04044062359617584 1104 44-45 0.12242505951103766 1104 46-47 0.336599721284891 1104 48-49 0.23985981747298268 1104 50-51 0.09528615240049021 1104 52-53 0.41362693909159276 1104 54-55 0.15047127797932092 1104 56-57 0.32747482537541117 1104 58-59 0.2935686814568399 1104 60-61 0.3726881240956246 1104 62-63 0.2626387017235885 1104 64-65 0.16002108505060875 1104 66-67 0.15878392124292873 1104 68-69 -0.046895317352699806 1104 70-71 0.25663562915944027 1104 72-73 0.5184986399707086 1104 74-75 0.3568790193156488 1104 76-77 0.3793967096575841 1104 78-79 0.12594569630748964 1104 80-81 0.19188408856324202 1104 82-83 0.25727588655389866 1104 84-85 0.08202865311406171 1104 86-87 0.21538929993388578 1104 88-89 0.40768225781202716 1104 90-91 0.3786145085647803 1104 92-93 0.167670342808524 1104 94-95 0.13856844872118756 1104 96-97 0.20679748084070582 1104 98-99 0.09763523226214232 1104 100 0.23974634154773256 1105 1 0.66543296995874 1105 2 0.535147024138503 1105 3 0.6137057534409891 1105 4 0.6858736866708597 1105 5 0.639753968253693 1105 6 0.6108773758323096 1105 7 0.6580209465865607 1105 8 0.41654695217253845 1105 9 0.6659614489921353 1105 10-11 0.5029537453841968 1105 12-13 0.5011571084890392 1105 14-15 0.8048005963541911 1105 16-17 0.6080708367620673 1105 18-19 0.6552365992830076 1105 20-21 0.7725093041414297 1105 22-23 0.7828767275317006 1105 24-25 0.7229683508638942 1105 26-27 0.5953012378825449 1105 28-29 0.7322707951721412 1105 30-31 0.7279630198173592 1105 32-33 0.6957874349076079 1105 34-35 0.7380020035782664 1105 36-37 0.6925318349594249 1105 38-39 0.6089240469382844 1105 40-41 0.6216663899832682 1105 42-43 0.78397394917382 1105 44-45 0.6174931156383323 1105 46-47 0.48464976334456367 1105 48-49 0.6871476398558869 1105 50-51 0.8005720239305703 1105 52-53 0.5681140124544015 1105 54-55 0.6423552156594212 1105 56-57 0.4941910836754815 1105 58-59 0.6989959450725394 1105 60-61 0.6855192321442374 1105 62-63 0.9134539434324651 1105 64-65 0.7196238896252609 1105 66-67 0.5619639008395154 1105 68-69 0.5352675788583525 1105 70-71 0.6087707167688379 1105 72-73 0.5914867111320987 1105 74-75 0.7711287463752328 1105 76-77 0.737676312506899 1105 78-79 0.747868404950303 1105 80-81 0.942468002081398 1105 82-83 0.626977039167862 1105 84-85 0.6035255075875945 1105 86-87 0.830353146514522 1105 88-89 0.8009368262082113 1105 90-91 0.7420279640799663 1105 92-93 0.4049087003336247 1105 94-95 0.5664646373990827 1105 96-97 0.6870109784155645 1105 98-99 0.6890378328026507 1105 100 1.088359491011694 1106 1 0.41340246632892885 1106 2 0.39449371604326444 1106 3 0.4109787873559334 1106 4 0.459243169363333 1106 5 0.3602127050121027 1106 6 0.34940345101107084 1106 7 0.4554597358998578 1106 8 0.4893842788972975 1106 9 0.64279162621132 1106 10-11 0.6093739272051195 1106 12-13 0.5755546223898946 1106 14-15 0.7250137137495827 1106 16-17 0.5612542097569246 1106 18-19 0.6381075755127554 1106 20-21 0.5412144531068463 1106 22-23 0.680088675366278 1106 24-25 0.564943761108502 1106 26-27 0.7328237394676549 1106 28-29 0.8681948583353787 1106 30-31 0.8492490267002637 1106 32-33 0.6017658727962782 1106 34-35 0.47565235705454967 1106 36-37 0.776454488622214 1106 38-39 0.7784037256741883 1106 40-41 0.5769622749606143 1106 42-43 0.627831784361959 1106 44-45 0.6552198556932964 1106 46-47 0.8132876596651428 1106 48-49 0.7970527419925091 1106 50-51 0.895546389800252 1106 52-53 0.759080459157083 1106 54-55 0.8261518720368883 1106 56-57 0.8003801352416318 1106 58-59 0.6806863487622792 1106 60-61 0.48162154611278396 1106 62-63 0.7466756140382387 1106 64-65 0.6299635490964519 1106 66-67 0.643497590334448 1106 68-69 0.5640916312416948 1106 70-71 0.5396310104688595 1106 72-73 0.4226515079310431 1106 74-75 0.706780877954877 1106 76-77 0.6382346647823312 1106 78-79 0.5429941530229598 1106 80-81 0.47911501378975885 1106 82-83 0.5249192380581107 1106 84-85 0.7379751590955657 1106 86-87 0.6258411222933766 1106 88-89 0.5701050470732234 1106 90-91 0.5414268399569906 1106 92-93 0.291916259011618 1106 94-95 0.4405502297775783 1106 96-97 0.2870565321718921 1106 98-99 0.4174586243252527 1106 100 0.36378237012500847 1107 1 0.14899435494179158 1107 2 0.23375537182927175 1107 3 0.14218682941497818 1107 4 0.3241596867820178 1107 5 0.2506015200794067 1107 6 0.24984252894740422 1107 7 0.37815544333529516 1107 8 0.30388425001059005 1107 9 0.2854445813213786 1107 10-11 0.2866863080474573 1107 12-13 0.35556271066766243 1107 14-15 0.360781811271103 1107 16-17 0.3542243409025687 1107 18-19 0.3465615589895634 1107 20-21 0.3804662874126947 1107 22-23 0.3266170709986085 1107 24-25 0.2744122457118934 1107 26-27 0.3607150099050003 1107 28-29 0.3856193195983053 1107 30-31 0.3628760415480343 1107 32-33 0.24487985973949122 1107 34-35 0.3043616990153808 1107 36-37 0.4442902965833895 1107 38-39 0.4259945743656246 1107 40-41 0.4198879205785033 1107 42-43 0.36386390637940025 1107 44-45 0.49364495246376094 1107 46-47 0.3043963314543845 1107 48-49 0.4858210128342719 1107 50-51 0.45087714258782086 1107 52-53 0.3595201148275464 1107 54-55 0.4758693600489039 1107 56-57 0.5156438708704982 1107 58-59 0.5218626154443555 1107 60-61 0.5116377226683255 1107 62-63 0.4485798257200244 1107 64-65 0.5804292028026126 1107 66-67 0.6525974805649639 1107 68-69 0.657964183093334 1107 70-71 0.622366003824915 1107 72-73 0.37085591026509235 1107 74-75 0.4590779488428538 1107 76-77 0.46369309681191595 1107 78-79 0.455336864906954 1107 80-81 0.578837701408915 1107 82-83 0.5911853423968978 1107 84-85 0.37014975034867703 1107 86-87 0.39195932669570865 1107 88-89 0.5727603131197903 1107 90-91 0.5847251240866349 1107 92-93 0.45801713139014666 1107 94-95 0.4990267448853274 1107 96-97 0.6394547065320495 1107 98-99 0.5835473642871207 1107 100 0.729507715321148 1108 1 0.4193110759478458 1108 2 0.5542653858022568 1108 3 0.425854738124805 1108 4 0.362133603875634 1108 5 0.4144138163066984 1108 6 0.4852407590359036 1108 7 0.3801838550726018 1108 8 0.5645083766989885 1108 9 0.5719769520712603 1108 10-11 0.4137298571857926 1108 12-13 0.4578426361450738 1108 14-15 0.5375319277126422 1108 16-17 0.5448810712239478 1108 18-19 0.5442024532606382 1108 20-21 0.4804581365044598 1108 22-23 0.6776801823167204 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-0.11001009375466708 2316 82-83 -0.06363684821477023 2316 84-85 -0.011770591045355161 2316 86-87 0.2099678615463496 2316 88-89 0.08792389775705445 2316 90-91 0.161353858083789 2316 92-93 0.13699294931333483 2316 94-95 0.36086173777295016 2316 96-97 0.29148884593461233 2316 98-99 0.3017408209016281 2316 100 -0.11091902721085845 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 1.0 1 21.0 2 13.0 3 25.0 4 13.0 5 13.0 6 35.0 7 240.0 8 504.0 9 711.0 10 1123.0 11 1682.0 12 2370.0 13 2816.0 14 2975.0 15 3200.0 16 3364.0 17 3760.0 18 4251.0 19 4718.0 20 5324.0 21 6167.0 22 6916.0 23 7526.0 24 7937.0 25 8556.0 26 9507.0 27 10933.0 28 13029.0 29 15199.0 30 18027.0 31 22067.0 32 27694.0 33 36331.0 34 49934.0 35 70112.0 36 109737.0 37 178007.0 38 192410.0 39 43750.0 40 41.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.57233366832322 19.485602717327563 12.704127274692473 24.237936339656745 2 15.28906672856836 20.03603781672426 39.99772465315325 24.67717080155413 3 18.116140086543496 26.182163954909182 29.943406800371164 25.758289158176158 4 12.329545846052078 16.103941145801034 38.287614755811575 33.27889825233531 5 12.728431569578824 37.81482013149206 35.42430593436881 14.032442364560305 6 31.467974277930153 36.7298390476267 18.454356644006968 13.347830030436175 7 27.464744263157108 31.888545602547897 22.927738799278526 17.71897133501647 8 25.887557320094697 35.50998523512433 20.734661636352364 17.867795808428607 9 26.87247480691135 14.67978915534695 19.797284186765836 38.65045185097586 10-11 24.73993629077766 25.652462690379952 29.582594948153734 20.025006070688647 12-13 25.118259224219493 23.369802334873384 29.0216173269282 22.49032111397893 14-15 22.565373033211138 24.864861761404576 26.2659359776315 26.303829227752786 16-17 22.604540243379457 27.65692877100665 26.883974811253758 22.854556174360134 18-19 22.361673445297484 27.27300390182736 28.162168120761443 22.203154532113718 20-21 23.094311499298943 26.688481179384656 28.00625152871925 22.21095579259715 22-23 22.69069374661534 26.560647051965958 27.81561959280152 22.933039608617175 24-25 22.51843464950482 26.625473031125463 27.878104809515797 22.977987509853918 26-27 22.409654739276228 26.746812821164923 27.856634791529483 22.986897648029366 28-29 22.423537396295252 26.913768522447423 27.93839927928466 22.72429480197267 30-31 22.957415354584988 26.795286552897107 27.857046488596733 22.390251603921175 32-33 22.685401554567765 26.696468864435214 27.809802572879693 22.80832700811733 34-35 22.8121476160183 26.882405087419937 27.805923840276325 22.499523456285438 36-37 22.779928321997524 26.887116906799076 27.684336599532177 22.648618171671227 38-39 22.70967230753508 27.062790593525616 27.662551515937935 22.564985583001377 40-41 22.75083788232957 26.746662394715642 27.901467219953226 22.601032503001562 42-43 22.610950164663052 26.816160235667713 27.66752706819376 22.90536253147548 44-45 22.489316177965314 27.07982804817683 27.659757737659767 22.771098036198087 46-47 22.523989451703304 26.76834110063541 27.527081253233188 23.180588194428097 48-49 22.511182134305024 26.879376475809213 27.616642391584534 22.99279899830122 50-51 22.509579093979035 27.05908897276058 27.66076056341263 22.770571369847755 52-53 22.709603925491002 26.983903695478173 27.486245738217153 22.820246640813668 54-55 22.52026610349924 26.945218646537793 27.71578419111863 22.818731058844335 56-57 22.602177763615327 26.955006473578898 27.440166045145673 23.002649717660095 58-59 22.57115804547707 26.973307586612393 27.60474534345517 22.850789024455366 60-61 22.78983826292266 27.20459212031291 27.437712162205614 22.56785745455882 62-63 22.589713784255032 27.08108871792162 27.618073471000525 22.71112402682282 64-65 22.464805878205024 26.829876128635117 27.633730772146052 23.0715872210138 66-67 22.55204412834518 26.958562785685043 27.433056181878417 23.056336904091356 68-69 22.383256054320206 26.991114109760122 27.638707454673344 22.986922381246323 70-71 22.414897129976595 26.793872306212403 27.72502907352371 23.066201490287288 72-73 22.347345637146436 27.123768809908444 27.423486894584116 23.10539865836101 74-75 22.52092491723352 27.100430988689777 27.33657029480086 23.042073799275848 76-77 22.32987243698357 26.92075154063764 27.608031239359942 23.14134478301885 78-79 22.427310051345 26.815720078748523 27.543093227661654 23.213876642244827 80-81 22.42654257014162 27.131894663409227 27.299838372123062 23.141724394326086 82-83 22.605744548064816 27.113527367471598 27.431144253298896 22.849583831164693 84-85 22.547663038423245 27.216016939713132 27.237375309036082 22.998944712827537 86-87 22.344900920205067 27.19689127203264 27.423856432622124 23.034351375140165 88-89 22.292878804058887 26.945362099671712 27.570133164308704 23.191625931960697 90-91 22.63492136405657 26.9476966185683 27.498062093398485 22.91931992397664 92-93 22.52256597533246 26.9817290245527 27.4586577551161 23.03704724499874 94-95 22.371451997032757 26.955463792513452 27.498616281713563 23.17446792874023 96-97 22.409664423981706 26.848607569937265 27.550176224630572 23.191551781450457 98-99 22.406288557808075 26.806407292173233 27.47213503105868 23.315169118960004 100 22.448649095645223 26.796190546196293 27.502653110916114 23.252507247242374 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 101.0 1 83.5 2 134.5 3 179.0 4 130.5 5 130.5 6 186.0 7 323.5 8 483.5 9 527.5 10 459.0 11 390.0 12 406.0 13 422.0 14 420.5 15 470.0 16 499.0 17 532.5 18 594.0 19 628.5 20 692.5 21 828.5 22 1026.5 23 1291.5 24 1619.5 25 2029.0 26 2608.0 27 3347.0 28 4279.0 29 5478.0 30 7082.5 31 8906.0 32 11006.5 33 13376.0 34 16084.0 35 18726.0 36 21660.5 37 24459.0 38 26424.0 39 28551.0 40 29820.0 41 30684.5 42 31853.0 43 32895.0 44 33747.0 45 34610.5 46 35096.0 47 35474.0 48 36016.0 49 36216.0 50 36153.0 51 34923.5 52 32889.5 53 31004.5 54 29096.0 55 26683.0 56 23963.5 57 21220.0 58 18504.0 59 15670.5 60 12803.0 61 10372.0 62 8277.5 63 6366.0 64 4753.0 65 3505.5 66 2609.0 67 2035.5 68 1601.5 69 1242.5 70 976.5 71 756.5 72 597.5 73 482.0 74 368.0 75 287.5 76 233.5 77 172.5 78 130.0 79 101.0 80 80.0 81 49.0 82 31.0 83 36.0 84 37.0 85 24.0 86 13.0 87 7.5 88 9.5 89 8.5 90 5.0 91 5.0 92 3.5 93 3.0 94 4.0 95 3.5 96 1.0 97 0.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15625017938347194 2 0.09666616534965713 3 0.030193825994014045 4 0.03180110190244065 5 0.030308631416044514 6 0.00413299519309698 7 0.005281049413401696 8 0.0060846873676149976 9 0.0075771578540111285 10-11 0.005797673812538819 12-13 0.008151184964163487 14-15 0.01968912987822589 16-17 0.01084911238187957 18-19 0.018942894635027824 20-21 0.02433874947045999 22-23 0.04391307392665541 24-25 0.022100043740865792 26-27 0.010160279849696741 28-29 0.02726628773223702 30-31 0.014522885886854664 32-33 0.02135380849766773 34-35 0.021239003075637256 36-37 0.02296108440609433 38-39 0.022042641029850558 40-41 0.027840314842389375 42-43 0.03690994318279663 44-45 0.03788578927005565 46-47 0.021526016630713435 48-49 0.013604442510610892 50-51 0.015154315708022257 52-53 0.025027582002642823 54-55 0.028701355507617918 56-57 0.02198523831883532 58-59 0.028529147374572208 60-61 0.027897717553404613 62-63 0.05016996942731611 64-65 0.03409721034305008 66-67 0.024740568447566644 68-69 0.03174369919142541 70-71 0.04609437694523437 72-73 0.044487101036807766 74-75 0.04207618717416786 76-77 0.02462576302553617 78-79 0.019000297346043058 80-81 0.024281346759444756 82-83 0.02049276783243919 84-85 0.02135380849766773 86-87 0.023018487117109566 88-89 0.018368867524875463 90-91 0.029160577195739803 92-93 0.02973460430589216 94-95 0.022616668140002915 96-97 0.015326523841067965 98-99 0.021526016630713435 100 0.04167436819706121 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 871039.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 82.31555782395559 #Duplication Level Percentage of deduplicated Percentage of total 1 88.47554183971504 72.82913580312864 2 8.450918426987068 13.912841288843717 3 1.6243168072385592 4.011196322120057 4 0.5940495858997703 1.9559809215371766 5 0.2721581491255775 1.1201424930803603 6 0.1626362047114762 0.8032493947917716 7 0.09633867158347816 0.5551120043989004 8 0.06486985732976037 0.4271838793647711 9 0.04119681857921552 0.305202519172838 >10 0.20581836889554778 2.8598920529018974 >50 0.009152926820968368 0.5131266208226687 >100 0.0025734370447888662 0.33869885055547244 >500 1.4296868960622628E-4 0.09532523423400147 >1k 2.8593737921245256E-4 0.2729126150477153 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1275 0.14637691308885134 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1044 0.1198568605998124 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 2.296108440609433E-4 7 0.0 0.0 0.0 0.0 2.296108440609433E-4 8 0.0 0.0 0.0 0.0 2.296108440609433E-4 9 0.0 0.0 0.0 0.0 2.296108440609433E-4 10-11 0.0 0.0 0.0 0.0 2.296108440609433E-4 12-13 0.0 0.0 0.0 0.0 2.296108440609433E-4 14-15 0.0 0.0 0.0 0.0 3.4441626609141497E-4 16-17 1.1480542203047166E-4 0.0 0.0 0.0 3.4441626609141497E-4 18-19 1.1480542203047166E-4 0.0 0.0 0.0 3.4441626609141497E-4 20-21 1.1480542203047166E-4 0.0 0.0 5.740271101523583E-5 3.4441626609141497E-4 22-23 1.1480542203047166E-4 0.0 0.0 2.296108440609433E-4 4.0181897710665077E-4 24-25 1.1480542203047166E-4 0.0 0.0 5.740271101523583E-4 4.592216881218866E-4 26-27 2.296108440609433E-4 0.0 0.0 7.462352431980658E-4 4.592216881218866E-4 28-29 2.296108440609433E-4 0.0 0.0 0.001033248798274245 4.592216881218866E-4 30-31 2.296108440609433E-4 0.0 0.0 0.003157149105837971 4.592216881218866E-4 32-33 2.296108440609433E-4 0.0 0.0 0.008151184964163487 4.592216881218866E-4 34-35 2.296108440609433E-4 1.1480542203047166E-4 0.0 0.015039510285991785 4.592216881218866E-4 36-37 2.296108440609433E-4 1.7220813304570748E-4 0.0 0.02652005248903895 4.592216881218866E-4 38-39 2.296108440609433E-4 2.296108440609433E-4 0.0 0.04442969832579253 4.592216881218866E-4 40-41 2.296108440609433E-4 2.296108440609433E-4 0.0 0.06423363362604889 4.592216881218866E-4 42-43 2.296108440609433E-4 3.4441626609141497E-4 0.0 0.08759653700924987 4.592216881218866E-4 44-45 2.296108440609433E-4 3.4441626609141497E-4 0.0 0.11779036300326393 4.592216881218866E-4 46-47 2.296108440609433E-4 3.4441626609141497E-4 0.0 0.15320783569966442 4.592216881218866E-4 48-49 2.296108440609433E-4 3.4441626609141497E-4 0.0 0.190347389726522 5.740271101523583E-4 50-51 2.296108440609433E-4 3.4441626609141497E-4 0.0 0.23581033685058878 5.740271101523583E-4 52-53 2.296108440609433E-4 4.0181897710665077E-4 0.0 0.28041224330942705 6.314298211675942E-4 54-55 2.296108440609433E-4 4.592216881218866E-4 0.0 0.33207468322313927 6.888325321828299E-4 56-57 2.296108440609433E-4 4.592216881218866E-4 0.0 0.3931511677433502 6.888325321828299E-4 58-59 2.296108440609433E-4 4.592216881218866E-4 0.0 0.462723253493816 6.888325321828299E-4 60-61 2.296108440609433E-4 4.592216881218866E-4 0.0 0.5358543073272265 6.888325321828299E-4 62-63 2.296108440609433E-4 4.592216881218866E-4 0.0 0.6209825277628211 6.888325321828299E-4 64-65 2.296108440609433E-4 4.592216881218866E-4 0.0 0.7076606213958272 6.888325321828299E-4 66-67 2.296108440609433E-4 4.592216881218866E-4 0.0 0.8086893927826424 6.888325321828299E-4 68-69 2.296108440609433E-4 4.592216881218866E-4 0.0 0.9220597470377331 6.888325321828299E-4 70-71 2.296108440609433E-4 4.592216881218866E-4 0.0 1.0562672853913544 6.888325321828299E-4 72-73 2.296108440609433E-4 4.592216881218866E-4 0.0 1.1903600183229455 6.888325321828299E-4 74-75 2.296108440609433E-4 4.592216881218866E-4 0.0 1.3305374386221511 6.888325321828299E-4 76-77 2.296108440609433E-4 5.740271101523583E-4 0.0 1.4866154098725775 6.888325321828299E-4 78-79 2.296108440609433E-4 5.740271101523583E-4 0.0 1.6769053968880843 6.888325321828299E-4 80-81 2.296108440609433E-4 5.740271101523583E-4 0.0 1.8770686501982117 8.036379542133015E-4 82-83 2.296108440609433E-4 5.740271101523583E-4 0.0 2.080905677013314 0.001033248798274245 84-85 2.296108440609433E-4 5.740271101523583E-4 0.0 2.2903681695079094 0.001033248798274245 86-87 2.296108440609433E-4 5.740271101523583E-4 0.0 2.521241873211188 0.001033248798274245 88 2.296108440609433E-4 5.740271101523583E-4 0.0 2.705504575570095 0.001033248798274245 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1045 0.0 44.566036 2 GTACCTG 1100 0.0 44.51696 1 TATCACG 50 0.0017068441 37.577354 7 ACCTGGG 1280 0.0 35.975094 3 GGTATCA 1175 0.0 32.859493 1 GTATCAA 1875 0.0 31.139042 1 CATGGGG 1605 0.0 28.103287 4 GTACATG 2710 0.0 27.973154 1 CAACGCA 2080 0.0 27.106836 5 CCTGGGG 1215 0.0 27.06965 4 TCAACGC 2070 0.0 27.010805 4 ATTACCG 70 0.008850941 26.847132 9 ATCAACG 2105 0.0 26.563221 3 TACATGG 2905 0.0 26.55723 2 ACATGGG 2915 0.0 26.435719 3 AACGCAG 2195 0.0 25.89179 6 TATCAAC 2285 0.0 24.70473 2 ATGGGGG 1015 0.0 24.534134 5 TCGACGC 80 2.030134E-6 23.51285 58-59 ACGCAGA 2480 0.0 22.349432 7 >>END_MODULE