FastQCFastQC Report
Mon 6 Jun 2016
ERR1211478_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211478_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences871039
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16350.18770686501982117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14840.17037124629321992No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11180.12835246183006732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG9800.041.2795372
GTACCTG10100.040.9848941
GGTATCA13700.039.8290441
ACCTGGG11600.034.842063
TAACGCA650.006155747528.920784
GTATCAA21450.028.7280731
GTACATG30550.025.7138881
ATGGGGG11300.024.9537725
TCAACGC24250.024.418684
TACATGG32750.024.2738042
CATGGGG17650.024.2303714
TAATACA4700.023.9980954
CAACGCA24750.023.9253735
ACATGGG31100.023.8759173
ATCAACG24950.023.5452233
ATCACGC1000.001759151223.4981353
TCACGCA1452.533915E-522.6878554
AACGCAG26650.022.218356
CCTGGGG10900.021.989084
TGGGGGG10900.021.9878166
Produced by FastQC (version 0.11.5)