FastQCFastQC Report
Mon 6 Jun 2016
ERR1211477_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211477_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12376095
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT487720.39408230140444134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT401130.3241167751217165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263450.21287005311449209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA222650.051.3508261
GTATCAA420750.039.8985251
ATCAACG502500.032.3947143
TCAACGC503100.032.3743674
CAACGCA509000.031.9322855
TACCTGG81400.031.3110032
AACGCAG529150.030.780636
GTACATG614100.030.2732831
TATCAAC550800.029.7103232
ACATGGG597100.029.0252653
TACATGG628700.028.8189142
ACGCAGA601550.026.9050647
CGCAGAG604900.026.6717058
CATGGGA344050.025.1591034
GTACCTG108800.024.5400581
CATGGGG212150.024.1662254
GCAGAGT675450.023.2968049
ACCTGGG112650.021.8173923
ATGGGAT174450.021.548465
GAGTACT378500.020.57005512-13
Produced by FastQC (version 0.11.5)