FastQCFastQC Report
Mon 6 Jun 2016
ERR1211477_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211477_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12376095
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT531410.4293842282238461No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT442060.3571885962413831No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT312560.252551390402223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA244200.049.897341
GTATCAA447950.038.6343771
TACCTGG79400.030.8583622
ATCAACG546150.030.8469093
TCAACGC548600.030.7091484
CAACGCA554450.030.410445
AACGCAG574400.029.41926
TATCAAC589200.028.7818452
GTACATG625450.028.2565961
ACATGGG606250.027.2773383
TACATGG638500.027.14142
ACGCAGA659950.025.455727
CGCAGAG660450.025.3083788
GTACCTG104700.024.2999971
CATGGGG208150.023.32164
CATGGGA354200.023.1914184
GCAGAGT732600.022.5651789
ACCTGGG111950.021.114383
GAGTACT413650.020.56235912-13
AGAGTAC702700.020.16194710-11
Produced by FastQC (version 0.11.5)