FastQCFastQC Report
Mon 6 Jun 2016
ERR1211476_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211476_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7778577
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217240.2792798734267206No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199170.2560494033805926No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123140.15830659000997227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA110550.050.1683351
GTATCAA189050.036.0607761
TACCTGG68650.030.2752672
TCAACGC218900.030.1389884
ATCAACG221900.029.8379783
CAACGCA222550.029.7276155
GTACCTG71300.029.7143441
AACGCAG231350.028.772796
TATCAAC240200.027.720212
GTACATG215750.025.8371161
ACGCAGA262300.025.1642997
TACATGG220450.024.593792
CGCAGAG267850.024.5551838
ACATGGG217550.023.9329593
ACCTGGG85150.023.6197243
CATGGGG127050.021.7105464
GCAGAGT306100.020.8280419
GAGTACT197800.019.62065912-13
CAGAGTA297800.019.23891810-11
ATGGGGG80300.019.0759875
Produced by FastQC (version 0.11.5)