FastQCFastQC Report
Mon 6 Jun 2016
ERR1211476_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211476_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7778577
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242700.3120107958049396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224760.28894745144259676No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153430.197246874331899No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127650.049.5506361
GTATCAA208150.034.9737631
TCAACGC248200.028.7603244
TACCTGG69200.028.6789042
ATCAACG250100.028.504993
CAACGCA251600.028.4271565
AACGCAG262250.027.3440516
TATCAAC265800.027.0171932
GTACCTG76100.026.8196331
GTACATG223900.025.7084051
TACATGG230200.024.1881472
ACGCAGA298450.023.9329287
ACATGGG224750.023.7744083
CGCAGAG305600.023.203838
ACCTGGG84650.022.6507263
CATGGGG125100.021.1489544
GAGTACT223000.020.4614712-13
GCAGAGT344950.020.175049
CAGAGTA337500.018.88021710-11
AGAGTAC315200.018.86674510-11
Produced by FastQC (version 0.11.5)