##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211476_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7778577 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12580951503083 33.0 31.0 34.0 30.0 34.0 2 32.341339424935946 34.0 31.0 34.0 30.0 34.0 3 32.35502663276329 34.0 31.0 34.0 30.0 34.0 4 35.987774627672906 37.0 35.0 37.0 35.0 37.0 5 35.911049283178656 37.0 35.0 37.0 35.0 37.0 6 35.92703678320598 37.0 35.0 37.0 35.0 37.0 7 35.85668226977762 37.0 35.0 37.0 35.0 37.0 8 35.85247005975515 37.0 35.0 37.0 35.0 37.0 9 37.51255801671694 39.0 37.0 39.0 35.0 39.0 10-11 37.47342206678677 39.0 37.0 39.0 34.5 39.0 12-13 37.391641684590894 39.0 37.0 39.0 34.0 39.0 14-15 38.6407391737589 40.0 38.0 41.0 34.0 41.0 16-17 38.57036184124679 40.0 38.0 41.0 34.0 41.0 18-19 38.560800773714774 40.0 38.0 41.0 34.0 41.0 20-21 38.50255541598418 40.0 38.0 41.0 34.0 41.0 22-23 38.39625518909179 40.0 38.0 41.0 34.0 41.0 24-25 38.29117870787935 40.0 38.0 41.0 34.0 41.0 26-27 38.121795991734736 40.0 38.0 41.0 33.0 41.0 28-29 38.15347440797976 40.0 38.0 41.0 33.5 41.0 30-31 38.08136918873465 40.0 37.5 41.0 33.0 41.0 32-33 37.907752214833124 40.0 37.0 41.0 33.0 41.0 34-35 37.791470792151316 40.0 37.0 41.0 33.0 41.0 36-37 37.73563345840762 40.0 37.0 41.0 33.0 41.0 38-39 37.64176635392309 40.0 37.0 41.0 32.5 41.0 40-41 37.487595803191255 40.0 37.0 41.0 32.0 41.0 42-43 37.34203055391751 40.0 36.5 41.0 31.0 41.0 44-45 37.16881847412451 40.0 36.0 41.0 31.0 41.0 46-47 36.92691388154928 39.0 36.0 41.0 30.5 41.0 48-49 36.72199895944978 39.0 36.0 41.0 30.0 41.0 50-51 36.530925836435124 39.0 35.0 41.0 30.0 41.0 52-53 36.342324630841865 39.0 35.0 40.0 29.0 41.0 54-55 36.15358548228037 39.0 35.0 40.0 28.0 41.0 56-57 35.965645001650046 38.5 35.0 40.0 28.0 41.0 58-59 35.71349110512115 38.0 34.0 40.0 28.0 41.0 60-61 35.41297187133328 38.0 34.0 40.0 27.0 41.0 62-63 35.10247349868749 38.0 34.0 40.0 26.0 41.0 64-65 34.796975847896086 37.0 33.5 40.0 26.0 41.0 66-67 34.464643147454865 36.5 33.0 39.5 26.0 41.0 68-69 34.07288312502402 36.0 33.0 39.0 25.5 40.5 70-71 33.691978751383445 35.5 32.0 39.0 25.0 40.0 72-73 33.28409444298102 35.0 32.0 38.0 24.5 40.0 74-75 32.87399211963833 35.0 31.0 37.5 23.5 39.5 76-77 31.32026860439898 33.5 29.5 36.0 21.5 38.5 78-79 32.147920628670256 34.5 31.0 36.0 23.0 39.0 80-81 32.10318648256616 35.0 31.0 36.0 23.0 38.5 82-83 31.806388045011317 34.0 31.0 36.0 22.5 37.0 84-85 31.496035200268636 34.0 31.0 35.0 21.5 37.0 86-87 31.189983977789254 34.0 30.0 35.0 20.0 36.5 88-89 30.9004718986519 34.0 30.0 35.0 20.0 36.0 90-91 30.608813990013854 34.0 30.0 35.0 19.5 36.0 92-93 30.394669230631774 34.0 29.0 35.0 19.0 35.0 94-95 30.168007014650623 34.0 29.0 35.0 18.5 35.0 96-97 29.95739484226999 34.0 29.0 35.0 18.0 35.0 98-99 29.787807525720964 34.0 29.0 35.0 18.0 35.0 100 27.9130457923088 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3450954748131636 1101 2 0.2808622224009625 1101 3 0.33958852734420475 1101 4 0.1981993763823553 1101 5 0.20168213689534298 1101 6 0.20273743603185324 1101 7 0.22255516093556338 1101 8 0.18613790905381222 1101 9 0.24837336406152133 1101 10-11 0.1996183184292306 1101 12-13 0.24139148078237582 1101 14-15 0.30798274959257554 1101 16-17 0.33667858536013995 1101 18-19 0.3178975166880136 1101 20-21 0.32258277009731273 1101 22-23 0.27059745303073157 1101 24-25 0.2900919595314235 1101 26-27 0.25542944686801405 1101 28-29 0.333673488998528 1101 30-31 0.33964066248738334 1101 32-33 0.3604944051056975 1101 34-35 0.32970479732173175 1101 36-37 0.417215656656019 1101 38-39 0.44535257898637326 1101 40-41 0.41024591863492077 1101 42-43 0.3874853652453041 1101 44-45 0.4101269162768375 1101 46-47 0.42974404894867035 1101 48-49 0.36628156742533236 1101 50-51 0.34174139186078634 1101 52-53 0.40304266205512107 1101 54-55 0.29487000346082226 1101 56-57 0.3249058200443429 1101 58-59 0.2897539107258069 1101 60-61 0.26172693672080527 1101 62-63 0.31902897912852524 1101 64-65 0.2911309296734217 1101 66-67 0.2967020074718931 1101 68-69 0.2593277066673281 1101 70-71 0.24309163806649536 1101 72-73 0.1224287395533139 1101 74-75 0.14432933301443285 1101 76-77 0.21538352087479495 1101 78-79 0.285760610920228 1101 80-81 0.2553587770184862 1101 82-83 0.1938802433599811 1101 84-85 0.14293053038737114 1101 86-87 0.10683849124396261 1101 88-89 0.13371763116084523 1101 90-91 0.07608629851496218 1101 92-93 0.023113459596931563 1101 94-95 0.08764110096408473 1101 96-97 0.06880189008496984 1101 98-99 2.4215241339220483E-5 1101 100 -0.06561057041340135 1102 1 0.3074163825401399 1102 2 0.24099537648268665 1102 3 0.33108218756837715 1102 4 0.2008483370690044 1102 5 0.1896351308590951 1102 6 0.1365798022193161 1102 7 0.17459798862805087 1102 8 0.15273069319981403 1102 9 0.20894976360388995 1102 10-11 0.21256394152308644 1102 12-13 0.22448152160731638 1102 14-15 0.3419016635675334 1102 16-17 0.31971134778007837 1102 18-19 0.268467867233511 1102 20-21 0.31259882129428007 1102 22-23 0.25934671615067373 1102 24-25 0.2911679161594236 1102 26-27 0.26418240659715053 1102 28-29 0.28575372975323887 1102 30-31 0.3649721198701741 1102 32-33 0.4447854548913952 1102 34-35 0.41896988062125473 1102 36-37 0.43561481297491156 1102 38-39 0.4277895052814884 1102 40-41 0.4549093757243696 1102 42-43 0.41209302058690156 1102 44-45 0.40041052880928873 1102 46-47 0.49056485842496755 1102 48-49 0.4875954496082926 1102 50-51 0.4911775223833743 1102 52-53 0.4320821098250818 1102 54-55 0.45864013760651545 1102 56-57 0.4712917927285005 1102 58-59 0.5172908846811808 1102 60-61 0.4886203149564352 1102 62-63 0.5878015625054331 1102 64-65 0.5363384985014577 1102 66-67 0.4334445978231898 1102 68-69 0.48982287847853456 1102 70-71 0.5348774347106016 1102 72-73 0.5142890560645057 1102 74-75 0.4650856695207537 1102 76-77 0.49040574108840573 1102 78-79 0.5737421530369886 1102 80-81 0.5392190054599908 1102 82-83 0.47979413438301677 1102 84-85 0.4283460496235634 1102 86-87 0.3597919710808881 1102 88-89 0.30398898843224487 1102 90-91 0.32611282702479016 1102 92-93 0.27695314065223187 1102 94-95 0.3288118252097867 1102 96-97 0.35395311749000413 1102 98-99 0.3076759231393531 1102 100 0.34371564516761666 1103 1 0.302509376493866 1103 2 0.26232140661393544 1103 3 0.3193787642019501 1103 4 0.1619857966580298 1103 5 0.18113478587105192 1103 6 0.22309749364948317 1103 7 0.18784430703635024 1103 8 0.23415436679145074 1103 9 0.2624506090729284 1103 10-11 0.2787064212039425 1103 12-13 0.2551266225096356 1103 14-15 0.37634841429972 1103 16-17 0.3700260204311192 1103 18-19 0.36949081600641165 1103 20-21 0.34999583109224375 1103 22-23 0.3763014841299821 1103 24-25 0.34672837464586337 1103 26-27 0.3689415797261333 1103 28-29 0.36755066283625837 1103 30-31 0.4732771626555703 1103 32-33 0.48353882549719174 1103 34-35 0.4763474480696601 1103 36-37 0.5164231050663162 1103 38-39 0.5339654025751415 1103 40-41 0.6117362421555441 1103 42-43 0.5686387007482168 1103 44-45 0.5276566760607295 1103 46-47 0.6257580517305925 1103 48-49 0.5413013585696476 1103 50-51 0.6079829101568137 1103 52-53 0.5234304935006904 1103 54-55 0.6424279344280492 1103 56-57 0.6407414775782954 1103 58-59 0.616524804914448 1103 60-61 0.5984307167089611 1103 62-63 0.6800746898851031 1103 64-65 0.662723990359666 1103 66-67 0.7140449992777178 1103 68-69 0.6646275189688993 1103 70-71 0.754889644918741 1103 72-73 0.6734170844520904 1103 74-75 0.6188632313148759 1103 76-77 0.7551593286966529 1103 78-79 0.7261596290692935 1103 80-81 0.7558196353783657 1103 82-83 0.6462174373894847 1103 84-85 0.6116444447987739 1103 86-87 0.6101299867768901 1103 88-89 0.5936988126067781 1103 90-91 0.6730221849496978 1103 92-93 0.6883952250677119 1103 94-95 0.7299012118798771 1103 96-97 0.6991325685807865 1103 98-99 0.679141514879813 1103 100 0.6425484547151115 1104 1 0.25599957102717497 1104 2 0.25265467477048986 1104 3 0.27154424485320305 1104 4 0.09094020927766877 1104 5 0.13696701953956136 1104 6 0.1938025837628743 1104 7 0.2055548908261713 1104 8 0.19987492060887035 1104 9 0.2374808049476087 1104 10-11 0.27141408268031597 1104 12-13 0.2656899943365616 1104 14-15 0.31880042928532504 1104 16-17 0.313040110074823 1104 18-19 0.29311628363796416 1104 20-21 0.18279846393567567 1104 22-23 0.27738914136395465 1104 24-25 0.2636579858559287 1104 26-27 0.28665473793494556 1104 28-29 0.3933608708291203 1104 30-31 0.3095665847594944 1104 32-33 0.28014980003661094 1104 34-35 0.3904474017219357 1104 36-37 0.29363409304128396 1104 38-39 0.33003227700347537 1104 40-41 0.4435983866708497 1104 42-43 0.48230975510526974 1104 44-45 0.42250891859932693 1104 46-47 0.3731246612188741 1104 48-49 0.39111227179233055 1104 50-51 0.36035535936053975 1104 52-53 0.3990370440777511 1104 54-55 0.4194576079971739 1104 56-57 0.4867797209748588 1104 58-59 0.49152234236739645 1104 60-61 0.3911189834103652 1104 62-63 0.4355577316537804 1104 64-65 0.5790472215291729 1104 66-67 0.4811879693839529 1104 68-69 0.47480523819248077 1104 70-71 0.4362239557763772 1104 72-73 0.5769972008349313 1104 74-75 0.626299413964766 1104 76-77 0.42106100728331697 1104 78-79 0.4763794574380924 1104 80-81 0.6971139258218315 1104 82-83 0.5738744083240128 1104 84-85 0.49661010666075 1104 86-87 0.546078230832137 1104 88-89 0.6315717015763838 1104 90-91 0.5095386388841305 1104 92-93 0.5310682307988834 1104 94-95 0.5070712438092215 1104 96-97 0.5078410408286054 1104 98-99 0.5889498902465249 1104 100 0.7460557192698296 1105 1 0.23946766201120084 1105 2 0.16269174313953272 1105 3 0.21265094702048515 1105 4 0.14114923800858747 1105 5 0.11903599116705976 1105 6 0.14077663232705362 1105 7 0.16304964784931286 1105 8 0.15906495991987413 1105 9 0.12314406793223753 1105 10-11 0.18012464352651847 1105 12-13 0.20380198241055325 1105 14-15 0.35016978952867106 1105 16-17 0.31585961807402896 1105 18-19 0.3523305632273974 1105 20-21 0.2816437121870905 1105 22-23 0.3045564620588408 1105 24-25 0.3666971753960695 1105 26-27 0.29471263913657 1105 28-29 0.38419792400501507 1105 30-31 0.42751522247988305 1105 32-33 0.4443877760144517 1105 34-35 0.51123606210124 1105 36-37 0.437012125787156 1105 38-39 0.4717204705540894 1105 40-41 0.47274060670135754 1105 42-43 0.43706862093190324 1105 44-45 0.5222506211461493 1105 46-47 0.49965485499689066 1105 48-49 0.5212484131111665 1105 50-51 0.5437583456465731 1105 52-53 0.5457483317130638 1105 54-55 0.5592316077174573 1105 56-57 0.5801114308365314 1105 58-59 0.6191259992536189 1105 60-61 0.6389749918523364 1105 62-63 0.5863169962585673 1105 64-65 0.6547541695593395 1105 66-67 0.641269225323839 1105 68-69 0.6525285634611819 1105 70-71 0.6705114662726714 1105 72-73 0.6750427546709332 1105 74-75 0.6670963643348244 1105 76-77 0.6337614430549401 1105 78-79 0.7308999842836172 1105 80-81 0.7591810986434737 1105 82-83 0.685488313372776 1105 84-85 0.7206196049481939 1105 86-87 0.6747307765560677 1105 88-89 0.6574322049088721 1105 90-91 0.7970215144536077 1105 92-93 0.7510384512828452 1105 94-95 0.7149032978923486 1105 96-97 0.7757106020820821 1105 98-99 0.7273377739419296 1105 100 0.8938811849050907 1106 1 0.23858517145841773 1106 2 0.22063386971896648 1106 3 0.21659216984725305 1106 4 0.1555405604223168 1106 5 0.15283308631080672 1106 6 0.14238715213921438 1106 7 0.14350984184794413 1106 8 0.14526999437975974 1106 9 0.14244526898118437 1106 10-11 0.1942260718085791 1106 12-13 0.18203155656559744 1106 14-15 0.2279462475352787 1106 16-17 0.2737281302614534 1106 18-19 0.29703904672907555 1106 20-21 0.2855305780970525 1106 22-23 0.2692847040523816 1106 24-25 0.2645913403726041 1106 26-27 0.27417265172871197 1106 28-29 0.2933571323466353 1106 30-31 0.33613961248076407 1106 32-33 0.35091645593954723 1106 34-35 0.33953984440395146 1106 36-37 0.28137956452089696 1106 38-39 0.29786435355322993 1106 40-41 0.3462409636898869 1106 42-43 0.3505679003952764 1106 44-45 0.46127207054103536 1106 46-47 0.4502301179938897 1106 48-49 0.4703418669719994 1106 50-51 0.4498317704891477 1106 52-53 0.5084496191223593 1106 54-55 0.40182612739797463 1106 56-57 0.4933157904506942 1106 58-59 0.5480953632711874 1106 60-61 0.5600052772361934 1106 62-63 0.4862195397494702 1106 64-65 0.6552498424377475 1106 66-67 0.5818074235479784 1106 68-69 0.5878624579972822 1106 70-71 0.6330702028443227 1106 72-73 0.6058659934983481 1106 74-75 0.5431552992774158 1106 76-77 0.5434780960689949 1106 78-79 0.6224472688354226 1106 80-81 0.5797471066862059 1106 82-83 0.6088625764513189 1106 84-85 0.6074896499865439 1106 86-87 0.5132615547571149 1106 88-89 0.5721244596547024 1106 90-91 0.5897889357818293 1106 92-93 0.6167420542826569 1106 94-95 0.7075931690767732 1106 96-97 0.7453844540781347 1106 98-99 0.7684610222355062 1106 100 0.8378288553528321 1107 1 0.2235047380543307 1107 2 0.21180656311116763 1107 3 0.27345178059242414 1107 4 0.18006945042453282 1107 5 0.16306687859708546 1107 6 0.17133552793751505 1107 7 0.17644804550180737 1107 8 0.1442919014289643 1107 9 0.2136361958979478 1107 10-11 0.18129780716631672 1107 12-13 0.20698386881645803 1107 14-15 0.2602903225303095 1107 16-17 0.26044737175885047 1107 18-19 0.20163783404063906 1107 20-21 0.24730484140705755 1107 22-23 0.3209820517366424 1107 24-25 0.3189377239778821 1107 26-27 0.2902401805596142 1107 28-29 0.2910229953899588 1107 30-31 0.22315787104541585 1107 32-33 0.23038986100562653 1107 34-35 0.2605873371990839 1107 36-37 0.3346904965424429 1107 38-39 0.2603769658831041 1107 40-41 0.31449666910116747 1107 42-43 0.329960906051177 1107 44-45 0.34937698705109455 1107 46-47 0.3202979029539179 1107 48-49 0.38411667301660657 1107 50-51 0.35709289931177324 1107 52-53 0.33909875937477096 1107 54-55 0.3690156198692378 1107 56-57 0.3892525274169927 1107 58-59 0.4438585019122243 1107 60-61 0.4078448574791622 1107 62-63 0.41983226452062183 1107 64-65 0.4720868834541463 1107 66-67 0.49312514453481526 1107 68-69 0.3555924869357625 1107 70-71 0.5020018336201204 1107 72-73 0.44737079205442143 1107 74-75 0.5173752736597734 1107 76-77 0.3878488658871966 1107 78-79 0.4942294450090188 1107 80-81 0.5795436159695271 1107 82-83 0.573202955578882 1107 84-85 0.4758807150979081 1107 86-87 0.4105057362622162 1107 88-89 0.4017956877010178 1107 90-91 0.2726736244081529 1107 92-93 0.4313977223659968 1107 94-95 0.6474719021793902 1107 96-97 0.6601662027071207 1107 98-99 0.603991617814156 1107 100 0.8341969158915141 1108 1 0.23944009253309417 1108 2 0.20171458198078085 1108 3 0.2473115063338014 1108 4 0.16218597175695493 1108 5 0.15455611957712279 1108 6 0.1581968903576012 1108 7 0.18350849348340859 1108 8 0.17160620292549567 1108 9 0.1799695814741824 1108 10-11 0.1966310584827511 1108 12-13 0.23293306962225557 1108 14-15 0.24994563374509227 1108 16-17 0.2602570882284212 1108 18-19 0.3202697040987488 1108 20-21 0.285225849625661 1108 22-23 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-0.06372291635846494 2316 68-69 -0.08360780800645529 2316 70-71 -0.2964770149271416 2316 72-73 -0.40927451752836674 2316 74-75 -0.3765500394240391 2316 76-77 -0.34718642919247955 2316 78-79 -0.284257027534764 2316 80-81 -0.1615607303882598 2316 82-83 -0.148607516195149 2316 84-85 -0.15555015723947463 2316 86-87 -0.2872967741784187 2316 88-89 -0.22487374307105767 2316 90-91 -0.10937740782593508 2316 92-93 0.02492371943709415 2316 94-95 0.07608413832315364 2316 96-97 0.021594756533264814 2316 98-99 -0.07882921853943614 2316 100 -0.3602817415764221 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 4.0 10 8.0 11 30.0 12 74.0 13 239.0 14 702.0 15 1872.0 16 4357.0 17 9036.0 18 16124.0 19 24903.0 20 34501.0 21 47476.0 22 60963.0 23 69342.0 24 75238.0 25 83018.0 26 96847.0 27 116029.0 28 140801.0 29 170301.0 30 205143.0 31 248981.0 32 304015.0 33 381043.0 34 499219.0 35 680964.0 36 999517.0 37 1572699.0 38 1659234.0 39 275858.0 40 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.748200647129366 20.45056587822202 13.747937866530084 24.053295608118532 2 14.981101880034888 21.750616858306554 39.98079312926623 23.28748813239233 3 17.08453380975711 27.082015332244264 31.233067931142074 24.600382926856547 4 11.776145389227992 17.98953090391441 38.93010142169847 31.304222285159128 5 12.176214829156413 38.39758769730769 36.06686927746623 13.359328196069658 6 29.93111555467907 38.72151230553212 18.325046853301664 13.022325286487145 7 25.98373712826909 33.12972282719577 23.46191340652667 17.42462663800847 8 24.866073253655653 36.7417984893243 20.9353698512285 17.45675840579155 9 25.899776218594333 15.312050166527975 20.917821302184862 37.87035231269283 10-11 23.93085521940581 26.71639041433928 29.83842674566312 19.514327620591786 12-13 24.54821749530795 24.60345253379892 29.506900812320815 21.34142915857232 14-15 21.90425061036228 26.109357791277247 27.06786215525025 24.918529443110224 16-17 21.53057670746835 28.884566586518588 27.74801989941787 21.83683680659519 18-19 21.322699728803233 28.054730452009615 29.55437980532199 21.068190013865166 20-21 22.128406631713965 27.35794781418766 29.558358416938567 20.955287137159807 22-23 21.81477549266244 27.35254323204252 29.250261921316838 21.5824193539782 24-25 21.606366101351078 27.438970027352696 29.412898287096397 21.541765584199833 26-27 21.628762517728198 27.540313332736822 29.32404781959867 21.506876329936304 28-29 21.61519452563678 27.577350269563123 29.425004422439667 21.382450782360433 30-31 21.927599460974243 27.42620728630974 29.41695663341852 21.229236619297502 32-33 21.673339359037133 27.531051112482867 29.33162062955684 21.46398889892316 34-35 21.913442438156867 27.633577599334764 29.311135935257575 21.141844027250794 36-37 21.895531612709558 27.60522865817893 29.13960347638725 21.359636252724265 38-39 21.7908241326153 27.748082559691372 29.142707903059073 21.318385404634256 40-41 21.94575409234972 27.54453102862729 29.234291810466523 21.275423068556467 42-43 21.771274974296247 27.630406450359583 29.062308314670766 21.536010260673404 44-45 21.78286043999975 27.791341747958885 28.972591023575166 21.453206788466197 46-47 21.754254668654486 27.709916529142802 28.743871852614873 21.79195694958784 48-49 21.7223284095867 27.767467607175366 28.809372327570674 21.70083165566726 50-51 21.819188981324317 27.91246813737654 28.636813277376653 21.631529603922488 52-53 21.832056112644405 27.992902032035992 28.46629059821368 21.708751257105924 54-55 21.773507924306887 27.930075417736454 28.571517645832202 21.72489901212446 56-57 21.860373120085857 27.95580005375155 28.40504524045847 21.778781585704117 58-59 21.8065035604071 27.935510271878623 28.562187689790242 21.695798477924036 60-61 21.958253420463674 28.00098740004262 28.50357145038202 21.537187729111686 62-63 21.7798807134052 28.028756073217732 28.56092664886957 21.630436564507498 64-65 21.730770466954734 27.84476496701709 28.544361406842395 21.880103159185783 66-67 21.712749582400765 27.89341113776249 28.411443761931405 21.98239551790534 68-69 21.664819268960233 27.91489078838774 28.499003613836493 21.921286328815533 70-71 21.69805068347669 27.769222100949243 28.46884340484382 22.063883810730246 72-73 21.638717693428013 27.983465895532827 28.273669629559535 22.10414678147962 74-75 21.590522455070285 27.99623081701136 28.224254945799192 22.18899178211916 76-77 21.54369993922239 27.886105774901704 28.321024072241475 22.249170213634432 78-79 21.516441774227296 27.832646725964427 28.338774443613396 22.312137056194878 80-81 21.50886786216196 28.008909067369935 28.127439930070715 22.354783140397394 82-83 21.60744870375636 28.00788371336349 28.1518861399127 22.232781442967447 84-85 21.555489279361254 27.99480302630047 28.106880371176405 22.342827323161874 86-87 21.43489976294923 27.991362787979394 28.17762155844006 22.396115890631318 88-89 21.439534273088647 27.853906907595018 28.178585655654338 22.527973163662008 90-91 21.60155449579026 27.878226019506165 28.128140201920633 22.392079282782944 92-93 21.435079180310066 27.919049539207176 28.090103880693295 22.555767399789463 94-95 21.287483178960557 27.837687732188538 28.16712192601874 22.707707162832165 96-97 21.262518151729882 27.785002250923807 28.142211835782987 22.810267761563324 98-99 21.22051095970783 27.726657347937277 28.07932423739281 22.973507454962082 100 20.414466718734207 28.159123516570105 28.345646012551246 23.080763752144442 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2330.0 1 1718.5 2 2171.0 3 3114.0 4 2271.0 5 1820.0 6 2747.0 7 5247.0 8 8140.0 9 9475.5 10 8252.5 11 6661.0 12 6707.5 13 7198.5 14 7768.5 15 8302.5 16 9032.0 17 9764.5 18 10770.0 19 12010.0 20 13686.5 21 16423.5 22 20199.0 23 25199.5 24 31850.5 25 40513.5 26 51377.0 27 63874.5 28 77451.0 29 92406.0 30 108847.5 31 125061.0 32 142510.0 33 161389.0 34 180264.0 35 197022.5 36 215765.0 37 234204.5 38 247018.0 39 257853.0 40 264747.5 41 269543.0 42 273381.5 43 278687.5 44 284928.0 45 289635.5 46 291320.5 47 291598.0 48 294288.0 49 293654.5 50 288726.0 51 275710.5 52 258190.5 53 242742.0 54 225336.0 55 205442.5 56 183372.0 57 160907.0 58 138717.0 59 116842.0 60 96266.0 61 78040.0 62 62273.0 63 47761.0 64 35221.0 65 26169.0 66 19395.5 67 14388.5 68 10961.5 69 8340.5 70 6312.0 71 4720.0 72 3609.5 73 2816.5 74 2170.5 75 1633.0 76 1144.5 77 751.0 78 469.5 79 311.5 80 221.5 81 157.5 82 114.5 83 78.5 84 59.5 85 48.0 86 29.5 87 19.0 88 15.0 89 11.5 90 7.5 91 8.5 92 6.0 93 3.0 94 4.0 95 4.0 96 2.5 97 3.0 98 3.5 99 3.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002892559911665077 2 0.06835440466810318 3 8.741958844143344E-4 4 8.227725970958441E-4 5 0.00150413115406584 6 5.528003386737702E-4 7 0.0 8 9.127633499032021E-4 9 5.1423287318490254E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.456376661181088E-4 18-19 0.001182735608325276 20-21 0.0032910903883833762 22-23 6.427910914811282E-6 24-25 1.9283732744433846E-5 26-27 1.1570239646660309E-4 28-29 8.356284189254666E-4 30-31 0.0012727263611326338 32-33 0.0029889785753872463 34-35 0.00395959312352375 36-37 0.0012791542720474451 38-39 0.001388428757599237 40-41 0.0052066078409971385 42-43 0.0068135855696999596 44-45 0.00531588232654893 46-47 0.006852153035188826 48-49 0.008465558674806459 50-51 0.008420563298402779 52-53 0.0037860395288238455 54-55 0.0021597780673765906 56-57 0.002526168989520834 58-59 0.003252522922894509 60-61 0.007385669641118163 62-63 0.014006417883373786 64-65 0.003946737301694128 66-67 0.010091820136253713 68-69 0.012470147174733888 70-71 0.00566298951594874 72-73 0.007623502344966181 74-75 0.003001834397216869 76-77 0.002821852891602153 78-79 0.007707065186858727 80-81 0.005579426674056193 82-83 0.016423312387342827 84-85 0.017464633955542257 86-87 0.02265195806379496 88-89 0.021327808415343836 90-91 0.02452248014000504 92-93 0.034524309523451396 94-95 0.03821393038855307 96-97 0.02275480463843194 98-99 0.01810099713610857 100 0.019270876922604225 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7778577.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.58569857108434 #Duplication Level Percentage of deduplicated Percentage of total 1 74.60841939844768 48.186368861331836 2 16.409304098338037 21.196127365130387 3 4.836410548690749 9.370888615912598 4 1.8039097073948245 4.660270744450203 5 0.8354986103490818 2.698063070228282 6 0.44997444763754685 1.7437148423885267 7 0.2659943392266222 1.2025601150433762 8 0.16882086971126678 0.8722731042944142 9 0.12276255657623042 0.7135834927363295 >10 0.46264352327614044 4.974753637933493 >50 0.022435431473547193 1.0060190016695951 >100 0.012523332972503168 1.497258483056131 >500 8.4321881123135E-4 0.352633312820567 >1k 3.8326424564250386E-4 0.5008765752434277 >5k 0.0 0.0 >10k+ 7.665284884938874E-5 1.024608777760798 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24270 0.3120107958049396 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22476 0.28894745144259676 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15343 0.197246874331899 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.2855821829622565E-5 0.0 0.0 0.0 3 0.0 1.2855821829622565E-5 0.0 0.0 0.0 4 0.0 1.2855821829622565E-5 0.0 0.0 0.0 5 0.0 1.2855821829622565E-5 0.0 0.0 0.0 6 0.0 1.2855821829622565E-5 0.0 2.571164365924513E-5 3.856746548886769E-5 7 0.0 1.2855821829622565E-5 0.0 2.571164365924513E-5 5.142328731849026E-5 8 0.0 1.2855821829622565E-5 0.0 3.856746548886769E-5 6.427910914811283E-5 9 0.0 1.2855821829622565E-5 0.0 7.713493097773538E-5 7.713493097773538E-5 10-11 0.0 1.2855821829622565E-5 0.0 8.999075280735795E-5 8.999075280735795E-5 12-13 0.0 2.571164365924513E-5 0.0 8.999075280735795E-5 8.999075280735795E-5 14-15 0.0 3.856746548886769E-5 0.0 8.999075280735795E-5 1.1570239646660308E-4 16-17 6.4279109148112825E-6 3.856746548886769E-5 0.0 1.2855821829622566E-4 1.6712568378509334E-4 18-19 1.2855821829622565E-5 3.856746548886769E-5 0.0 1.414140401258482E-4 1.6712568378509334E-4 20-21 1.2855821829622565E-5 6.427910914811283E-5 0.0 1.9926523835914976E-4 1.6712568378509334E-4 22-23 1.2855821829622565E-5 6.427910914811283E-5 0.0 2.6997225842207383E-4 2.1212106018877233E-4 24-25 3.856746548886769E-5 6.427910914811283E-5 0.0 3.4710718939980924E-4 2.571164365924513E-4 26-27 5.142328731849026E-5 6.427910914811283E-5 0.0 5.0137705135528E-4 2.571164365924513E-4 28-29 5.142328731849026E-5 6.427910914811283E-5 0.0 0.0012534426283882 2.571164365924513E-4 30-31 5.142328731849026E-5 6.427910914811283E-5 0.0 0.004698802878727048 2.571164365924513E-4 32-33 5.142328731849026E-5 6.427910914811283E-5 0.0 0.012952240493344733 2.699722584220739E-4 34-35 6.427910914811282E-5 6.427910914811283E-5 0.0 0.026405858038044745 2.699722584220739E-4 36-37 7.713493097773538E-5 6.427910914811283E-5 0.0 0.048749276377928766 2.699722584220739E-4 38-39 7.713493097773538E-5 6.427910914811283E-5 0.0 0.08137735218151083 2.699722584220739E-4 40-41 7.713493097773538E-5 6.427910914811283E-5 0.0 0.12002195260135627 2.699722584220739E-4 42-43 7.713493097773538E-5 6.427910914811283E-5 0.0 0.15718813351079508 2.892559911665077E-4 44-45 8.999075280735795E-5 7.713493097773538E-5 0.0 0.20581527958134244 3.149676348257528E-4 46-47 8.999075280735795E-5 7.713493097773538E-5 0.0 0.26506776239407287 3.599630112294318E-4 48-49 8.999075280735795E-5 7.713493097773538E-5 0.0 0.3292183133238894 3.985304767182995E-4 50-51 8.999075280735795E-5 7.713493097773538E-5 0.0 0.39839549058908846 3.985304767182995E-4 52-53 1.0284657463698052E-4 7.713493097773538E-5 0.0 0.46904465945377927 4.2424212037754465E-4 54-55 1.1570239646660308E-4 9.641866372216924E-5 0.0 0.5504220116352901 4.5638167495160107E-4 56-57 1.1570239646660308E-4 1.1570239646660308E-4 0.0 0.6503310309842019 4.628095858664123E-4 58-59 1.1570239646660308E-4 1.1570239646660308E-4 0.0 0.7627744766169957 4.692374967812236E-4 60-61 1.1570239646660308E-4 1.2855821829622566E-4 0.0 0.8825778802472484 4.7566540769603487E-4 62-63 1.1570239646660308E-4 1.2855821829622566E-4 0.0 1.0055630997803326 4.7566540769603487E-4 64-65 1.1570239646660308E-4 1.2855821829622566E-4 0.0 1.1339927598582622 4.7566540769603487E-4 66-67 1.1570239646660308E-4 1.2855821829622566E-4 0.0 1.2857557365569563 4.820933186108462E-4 68-69 1.1570239646660308E-4 1.2855821829622566E-4 0.0 1.458589405234402 4.885212295256575E-4 70-71 1.1570239646660308E-4 1.3498612921103691E-4 0.0 1.6484248982815237 5.206607840997139E-4 72-73 1.1570239646660308E-4 1.414140401258482E-4 0.0 1.8383503820814528 6.299352696515056E-4 74-75 1.1570239646660308E-4 1.414140401258482E-4 0.0 2.032955385027364 6.363631805663169E-4 76-77 1.2855821829622566E-4 1.414140401258482E-4 0.0 2.2551232699759867 6.492190023959395E-4 78-79 1.2855821829622566E-4 1.414140401258482E-4 0.0 2.5111083942474313 6.556469133107508E-4 80-81 1.2855821829622566E-4 1.414140401258482E-4 0.0 2.7820062718412375 7.134981115440523E-4 82-83 1.2855821829622566E-4 1.414140401258482E-4 0.0 3.066306343692426 7.713493097773539E-4 84-85 1.2855821829622566E-4 1.414140401258482E-4 0.0 3.3559480095138223 7.713493097773539E-4 86-87 1.2855821829622566E-4 1.414140401258482E-4 0.0 3.6597310793478037 7.713493097773539E-4 88 1.2855821829622566E-4 1.414140401258482E-4 0.0 3.906832830734979 7.713493097773539E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12765 0.0 49.550636 1 GTATCAA 20815 0.0 34.973763 1 TCAACGC 24820 0.0 28.760324 4 TACCTGG 6920 0.0 28.678904 2 ATCAACG 25010 0.0 28.50499 3 CAACGCA 25160 0.0 28.427156 5 AACGCAG 26225 0.0 27.344051 6 TATCAAC 26580 0.0 27.017193 2 GTACCTG 7610 0.0 26.819633 1 GTACATG 22390 0.0 25.708405 1 TACATGG 23020 0.0 24.188147 2 ACGCAGA 29845 0.0 23.932928 7 ACATGGG 22475 0.0 23.774408 3 CGCAGAG 30560 0.0 23.20383 8 ACCTGGG 8465 0.0 22.650726 3 CATGGGG 12510 0.0 21.148954 4 GAGTACT 22300 0.0 20.46147 12-13 GCAGAGT 34495 0.0 20.17504 9 CAGAGTA 33750 0.0 18.880217 10-11 AGAGTAC 31520 0.0 18.866745 10-11 >>END_MODULE