FastQCFastQC Report
Mon 6 Jun 2016
ERR1211475_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211475_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4185
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCACTGTACCGTATGCGAATCTTTGCACCCAACCATGTGGTGGCCAAGTC150.35842293906810035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110.2628434886499403No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC90.21505376344086022No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.19115890083632017No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT80.19115890083632017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.19115890083632017No Hit
GGTCACAAGCCTCGTGCTCTGCATTGTGAGCCATGTTGTAGGGGACGATT70.16726403823178018No Hit
ACATAACATTCTTCCCTCAACATTTCCTGGGCCTTTCAGGAATACCACGA60.14336917562724014No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG60.14336917562724014No Hit
CGATACATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAG50.11947431302270012No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.11947431302270012No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)