FastQCFastQC Report
Mon 6 Jun 2016
ERR1211474_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211474_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6693224
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT195300.29178763477809794No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173530.25926220308777953No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110210.16465906415204393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA108150.049.845551
GTATCAA178850.036.3276751
TCAACGC220050.028.9766524
ATCAACG222500.028.6379643
CAACGCA223750.028.4533585
TATCAAC230250.027.877092
AACGCAG231250.027.5855226
GTACATG225500.027.0586361
TACCTGG53900.026.2598442
TACATGG228500.025.8063032
ACATGGG231250.024.750163
ACGCAGA258600.024.5241747
GTACCTG61000.024.0808721
CGCAGAG266550.023.6348138
CATGGGG149450.023.1718834
GCAGAGT298200.020.655529
GAGTACT171850.019.95899612-13
ACCTGGG70900.019.9495333
ATGGGGG92150.019.8850445
AGAGTAC271300.019.3095710-11
Produced by FastQC (version 0.11.5)