##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211474_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6693224
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35646080274618	31.0	31.0	34.0	28.0	34.0
2	31.401476627705872	31.0	31.0	34.0	28.0	34.0
3	31.498258686695678	33.0	31.0	34.0	28.0	34.0
4	35.03678959496948	37.0	35.0	37.0	32.0	37.0
5	35.11724648689481	37.0	35.0	37.0	32.0	37.0
6	35.23261555268433	37.0	35.0	37.0	32.0	37.0
7	35.16217356538493	37.0	35.0	37.0	32.0	37.0
8	35.12800243948208	37.0	35.0	37.0	32.0	37.0
9	36.70174657235437	39.0	37.0	39.0	32.0	39.0
10-11	36.69111111476323	39.0	37.0	39.0	32.0	39.0
12-13	36.63163021288396	39.0	37.0	39.0	32.0	39.0
14-15	37.82628535964133	40.0	38.0	41.0	32.0	41.0
16-17	37.806829264940184	40.0	38.0	41.0	32.0	41.0
18-19	37.80830277008509	40.0	38.0	41.0	32.0	41.0
20-21	37.75805239746944	40.0	38.0	41.0	32.0	41.0
22-23	37.61326268178085	40.0	37.0	41.0	32.0	41.0
24-25	37.52870201266236	40.0	37.0	41.0	31.5	41.0
26-27	37.38628350104524	40.0	37.0	41.0	31.0	41.0
28-29	37.22760630751339	40.0	37.0	41.0	31.0	41.0
30-31	37.05552219677692	40.0	36.0	41.0	30.0	41.0
32-33	36.90322937047976	39.0	36.0	41.0	30.0	41.0
34-35	36.73989022330644	39.0	36.0	41.0	30.0	41.0
36-37	36.57066661746268	39.0	35.0	41.0	30.0	41.0
38-39	36.44343413577672	39.0	35.0	40.0	29.0	41.0
40-41	36.40015715894164	39.0	35.0	40.0	29.0	41.0
42-43	36.34427056378212	39.0	35.0	40.0	29.0	41.0
44-45	36.15491891501017	39.0	35.0	40.0	28.0	41.0
46-47	36.10813533209108	39.0	35.0	40.0	28.0	41.0
48-49	36.070002587691675	39.0	35.0	41.0	27.5	41.0
50-51	35.10648239174425	38.0	34.0	40.0	26.0	40.5
52-53	35.32090618810905	38.0	34.5	39.5	26.5	40.5
54-55	35.901010708740664	39.0	35.0	40.5	27.5	41.0
56-57	35.854169604961676	39.0	35.0	41.0	27.0	41.0
58-59	35.68582255726089	38.5	34.5	40.5	27.0	41.0
60-61	35.48719443723981	38.0	34.0	40.0	27.0	41.0
62-63	35.23143525452009	38.0	34.0	40.0	26.0	41.0
64-65	34.93896446615264	37.0	34.0	40.0	26.0	41.0
66-67	34.62795358410237	37.0	34.0	40.0	26.0	41.0
68-69	34.33849860694936	36.0	33.0	39.0	26.0	41.0
70-71	33.98978190480402	36.0	33.0	39.0	25.5	41.0
72-73	33.64299528896687	35.0	33.0	38.5	25.0	40.0
74-75	33.27705385327012	35.0	32.5	37.5	24.5	40.0
76-77	32.93640673014978	35.0	32.0	37.0	24.0	39.0
78-79	32.62386967775171	35.0	32.0	36.5	24.0	39.0
80-81	32.287974674088304	35.0	32.0	36.0	23.0	38.5
82-83	31.992991045869672	35.0	31.0	36.0	23.0	37.0
84-85	31.73757153503304	35.0	31.0	35.0	22.5	37.0
86-87	31.446219863551555	34.5	31.0	35.0	21.0	36.5
88-89	31.224336806896048	34.0	31.0	35.0	20.0	36.0
90-91	30.994159167540186	34.0	30.5	35.0	20.0	36.0
92-93	30.85022651266415	34.0	30.0	35.0	20.0	35.5
94-95	30.687841016526566	34.0	30.0	35.0	19.0	35.0
96-97	30.58607653053297	34.0	30.0	35.0	19.0	35.0
98-99	30.409919718808155	34.0	30.0	35.0	18.5	35.0
100	29.008058448365095	33.0	27.0	35.0	15.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.433397202201494
1101	2	0.43270096457345986
1101	3	0.3806801667150559
1101	4	0.4241705321179623
1101	5	0.40734965394050704
1101	6	0.4059894707197884
1101	7	0.49775683007399607
1101	8	0.510221692370898
1101	9	0.4448718628297712
1101	10-11	0.4820416947169761
1101	12-13	0.4420447845776536
1101	14-15	0.5465698564148624
1101	16-17	0.5353445035933007
1101	18-19	0.479905551323462
1101	20-21	0.4663920151676493
1101	22-23	0.49231524580346075
1101	24-25	0.4838068926885697
1101	26-27	0.5069383289063367
1101	28-29	0.536898425105754
1101	30-31	0.5672335292164874
1101	32-33	0.458486816196519
1101	34-35	0.41203886954968993
1101	36-37	0.4242765507495321
1101	38-39	0.4093025810341473
1101	40-41	0.29506443906529256
1101	42-43	0.2601268925155793
1101	44-45	0.20585048535822636
1101	46-47	0.2832620534185182
1101	48-49	0.30167290620362763
1101	50-51	0.28114149010191625
1101	52-53	0.4242029356277115
1101	54-55	0.5127746299124212
1101	56-57	0.4755698213993682
1101	58-59	0.368493629422602
1101	60-61	0.4069376393691968
1101	62-63	0.4151417832383544
1101	64-65	0.37767116470246265
1101	66-67	0.33139124600965886
1101	68-69	0.3190404864955596
1101	70-71	0.2616139289553914
1101	72-73	0.2641379007101321
1101	74-75	0.17654730960315135
1101	76-77	0.15958891851051504
1101	78-79	0.11804935757641033
1101	80-81	0.1324488838810467
1101	82-83	0.0463836954347947
1101	84-85	-0.02422611534454333
1101	86-87	-0.026256324719650337
1101	88-89	-0.029204384450686405
1101	90-91	-0.10485832080738788
1101	92-93	-0.19401362035423375
1101	94-95	-0.2086447362057946
1101	96-97	-0.37341053593644347
1101	98-99	-0.3274518198548435
1101	100	-0.2622552232118842
1102	1	0.4708557633088013
1102	2	0.4685625192284846
1102	3	0.4053881470396945
1102	4	0.38928446938385264
1102	5	0.4303162432815313
1102	6	0.4376049586535089
1102	7	0.4439898382116141
1102	8	0.43492852053236675
1102	9	0.46274618976433857
1102	10-11	0.5078564612770506
1102	12-13	0.48610976897033
1102	14-15	0.5929605951294121
1102	16-17	0.6037324740164323
1102	18-19	0.5835071293425642
1102	20-21	0.5718698544856267
1102	22-23	0.6208505030139904
1102	24-25	0.5918449431291535
1102	26-27	0.47527841639939794
1102	28-29	0.5466779561633928
1102	30-31	0.5465086068273806
1102	32-33	0.5382355915936685
1102	34-35	0.5871503880198858
1102	36-37	0.5711948700187222
1102	38-39	0.5364910211290308
1102	40-41	0.5488328856037015
1102	42-43	0.4956678470328981
1102	44-45	0.3910405184260597
1102	46-47	0.3539577861180092
1102	48-49	0.4669911311485677
1102	50-51	0.4454903013935194
1102	52-53	0.49831302076584905
1102	54-55	0.5283771319775852
1102	56-57	0.5814696007946694
1102	58-59	0.5585984506876471
1102	60-61	0.5250713094553561
1102	62-63	0.4909413559968385
1102	64-65	0.5329530494501782
1102	66-67	0.4442807436838834
1102	68-69	0.42301519171498825
1102	70-71	0.42079105887886215
1102	72-73	0.3855294288525144
1102	74-75	0.48371534427217
1102	76-77	0.41134821283989
1102	78-79	0.38500752339091804
1102	80-81	0.33089389123593804
1102	82-83	0.3992487060004315
1102	84-85	0.29490311895078847
1102	86-87	0.27060530233305613
1102	88-89	0.25536586513156934
1102	90-91	0.11911643744515743
1102	92-93	0.15373635530935204
1102	94-95	0.16282587944129645
1102	96-97	0.02101158075609888
1102	98-99	0.00985130379768151
1102	100	0.02549381092712011
1103	1	0.41401268688222714
1103	2	0.4561425662075571
1103	3	0.3957164692010373
1103	4	0.4306476205930778
1103	5	0.46254852524913304
1103	6	0.38544926333540985
1103	7	0.44493192801943593
1103	8	0.48185462639188614
1103	9	0.4263843002925398
1103	10-11	0.4621702006988997
1103	12-13	0.42037142973926933
1103	14-15	0.5440299308900265
1103	16-17	0.5232751153381585
1103	18-19	0.5033705940684783
1103	20-21	0.5716121599450048
1103	22-23	0.5704748456829449
1103	24-25	0.5641432979209853
1103	26-27	0.5109816940555945
1103	28-29	0.5478136878112494
1103	30-31	0.48874983993592735
1103	32-33	0.5562864432790207
1103	34-35	0.5732697086827656
1103	36-37	0.5998784140120961
1103	38-39	0.5030546435735204
1103	40-41	0.4601816803855101
1103	42-43	0.4085375295179503
1103	44-45	0.4482006949136448
1103	46-47	0.4605628121385763
1103	48-49	0.5164841041921804
1103	50-51	0.47588955072274786
1103	52-53	0.4867002213565641
1103	54-55	0.5479149973418345
1103	56-57	0.5749652842081545
1103	58-59	0.5463235400800741
1103	60-61	0.5057470744050221
1103	62-63	0.555840823211625
1103	64-65	0.4199410176766776
1103	66-67	0.4518702328372868
1103	68-69	0.4258798703627562
1103	70-71	0.4131720069405702
1103	72-73	0.3687089041276508
1103	74-75	0.43512342027629813
1103	76-77	0.3956992534415207
1103	78-79	0.30772810809801143
1103	80-81	0.2797960173096783
1103	82-83	0.36946638384031516
1103	84-85	0.3174364278948296
1103	86-87	0.3135797652939907
1103	88-89	0.3930935608642194
1103	90-91	0.2905509150318366
1103	92-93	0.20007652453277913
1103	94-95	0.20134084109394124
1103	96-97	0.2372436716854054
1103	98-99	0.1992227771548052
1103	100	0.13659809124323985
1104	1	0.5030703445168001
1104	2	0.5024400051620717
1104	3	0.4516827073596339
1104	4	0.454680026979581
1104	5	0.3914148479494486
1104	6	0.4106120584137827
1104	7	0.4129233551605864
1104	8	0.5151407660675815
1104	9	0.5439772619075427
1104	10-11	0.5435651829532304
1104	12-13	0.5207169302471826
1104	14-15	0.5729567264350521
1104	16-17	0.5145538982430651
1104	18-19	0.48040656375034985
1104	20-21	0.5381641468103524
1104	22-23	0.5490836094743585
1104	24-25	0.5361839779772097
1104	26-27	0.608076687841411
1104	28-29	0.6149661940203757
1104	30-31	0.5996876416398393
1104	32-33	0.5446791806033247
1104	34-35	0.5467291206760905
1104	36-37	0.6000520605971289
1104	38-39	0.5887721051818957
1104	40-41	0.49013484526030027
1104	42-43	0.3475785305674677
1104	44-45	0.43097163809670747
1104	46-47	0.5136724234826815
1104	48-49	0.5158613367309215
1104	50-51	0.47301732943338237
1104	52-53	0.5583870107964302
1104	54-55	0.6275330805038237
1104	56-57	0.5678505790060981
1104	58-59	0.5919618806616072
1104	60-61	0.5915984518101922
1104	62-63	0.506339070898413
1104	64-65	0.5337736596830567
1104	66-67	0.5074486883874698
1104	68-69	0.3125233515575587
1104	70-71	0.3641333683837473
1104	72-73	0.47639175960131297
1104	74-75	0.4650291835671183
1104	76-77	0.5186677569856002
1104	78-79	0.4002985406139956
1104	80-81	0.42597030767958444
1104	82-83	0.4773908553309205
1104	84-85	0.35517151671083624
1104	86-87	0.3783079628694104
1104	88-89	0.3962874217100314
1104	90-91	0.3753007605481926
1104	92-93	0.4110521519701571
1104	94-95	0.4730851586920828
1104	96-97	0.5170194529208274
1104	98-99	0.29735981903971265
1104	100	0.36587502911103087
1105	1	0.4057186839784528
1105	2	0.437982948390772
1105	3	0.38457747235964845
1105	4	0.31336541250080074
1105	5	0.3258678563074753
1105	6	0.35808536189849605
1105	7	0.35612515756928786
1105	8	0.42437129491464987
1105	9	0.4769269926924551
1105	10-11	0.46571021611298846
1105	12-13	0.44947636274230973
1105	14-15	0.5176417255834522
1105	16-17	0.5230528911233137
1105	18-19	0.5167062978918366
1105	20-21	0.4881885955914811
1105	22-23	0.6140195648075562
1105	24-25	0.5274730736742512
1105	26-27	0.5666991045594543
1105	28-29	0.5363161654235071
1105	30-31	0.569670496712213
1105	32-33	0.496326255998504
1105	34-35	0.5309746075454242
1105	36-37	0.5026466068772919
1105	38-39	0.5798313339664887
1105	40-41	0.6069771166165552
1105	42-43	0.603406735959517
1105	44-45	0.599864565627179
1105	46-47	0.5654785918456611
1105	48-49	0.6567990325007216
1105	50-51	0.643612107595871
1105	52-53	0.614763694105271
1105	54-55	0.6433368796216143
1105	56-57	0.734322740081943
1105	58-59	0.6897318674668824
1105	60-61	0.6693657665454467
1105	62-63	0.651196376789315
1105	64-65	0.5689008637011028
1105	66-67	0.5597850200677215
1105	68-69	0.4920453060646466
1105	70-71	0.6071962466033085
1105	72-73	0.5527497516117776
1105	74-75	0.5861447881922146
1105	76-77	0.5691950181094256
1105	78-79	0.568664419555013
1105	80-81	0.5847546975725706
1105	82-83	0.5891518330241929
1105	84-85	0.5210859216453905
1105	86-87	0.5224364957402727
1105	88-89	0.5558632456178287
1105	90-91	0.4690692015690239
1105	92-93	0.46715133135297293
1105	94-95	0.4582525317057282
1105	96-97	0.4258216883719683
1105	98-99	0.4957233117327817
1105	100	0.6277069639682189
1106	1	0.36374001358970887
1106	2	0.3283336378131274
1106	3	0.3202914857881538
1106	4	0.3058370772382517
1106	5	0.3429860129411253
1106	6	0.296326365393071
1106	7	0.28565724178811536
1106	8	0.3644286435360584
1106	9	0.3810171230555568
1106	10-11	0.3649110521141168
1106	12-13	0.39210972596074356
1106	14-15	0.5296824252908792
1106	16-17	0.4834006958872834
1106	18-19	0.4543597034381577
1106	20-21	0.4090494956781967
1106	22-23	0.46965980502225335
1106	24-25	0.49241045422849794
1106	26-27	0.5427264021513665
1106	28-29	0.5140342870160737
1106	30-31	0.5474754696140209
1106	32-33	0.5689469626408439
1106	34-35	0.5453728465476146
1106	36-37	0.5758805691663369
1106	38-39	0.6043320718085923
1106	40-41	0.5169807966635958
1106	42-43	0.4819422385168579
1106	44-45	0.5002996468851109
1106	46-47	0.5377170904157964
1106	48-49	0.6245459493617176
1106	50-51	0.633884101191704
1106	52-53	0.6520769152240504
1106	54-55	0.6197637775399016
1106	56-57	0.6197380795736152
1106	58-59	0.5569764982269518
1106	60-61	0.557035501384135
1106	62-63	0.5659526506463877
1106	64-65	0.6516357067822867
1106	66-67	0.6915889835223581
1106	68-69	0.6598341549356732
1106	70-71	0.6477224346830397
1106	72-73	0.4971603502938535
1106	74-75	0.5657768824542018
1106	76-77	0.6304560903226459
1106	78-79	0.5086258267111603
1106	80-81	0.5771871976660279
1106	82-83	0.4856349872790098
1106	84-85	0.5335734684981723
1106	86-87	0.5974942988694814
1106	88-89	0.599046673237094
1106	90-91	0.6281279085823002
1106	92-93	0.628722678448117
1106	94-95	0.6703308565952355
1106	96-97	0.5855316244256557
1106	98-99	0.5822698808219968
1106	100	0.5894399362366478
1107	1	0.3041814681693751
1107	2	0.2733108217995799
1107	3	0.2700270082043019
1107	4	0.28684944963323034
1107	5	0.22075032587333254
1107	6	0.25774594482572866
1107	7	0.1970219687016055
1107	8	0.234656050978316
1107	9	0.20309305098518138
1107	10-11	0.296058156597347
1107	12-13	0.3012930538056935
1107	14-15	0.38547731638557536
1107	16-17	0.35069570459030075
1107	18-19	0.2547013320781346
1107	20-21	0.3424639944315544
1107	22-23	0.4269820988548716
1107	24-25	0.3160105859498188
1107	26-27	0.3759082246098515
1107	28-29	0.43440536477869074
1107	30-31	0.4058498404095232
1107	32-33	0.4184993924286715
1107	34-35	0.3809910429064658
1107	36-37	0.4381543613289196
1107	38-39	0.5335633838469747
1107	40-41	0.46972295218883175
1107	42-43	0.4516233788470956
1107	44-45	0.5471987389489001
1107	46-47	0.5074163894702295
1107	48-49	0.5392388034803659
1107	50-51	0.5060229730927333
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2314	1	-0.4017897123914693
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2314	4	-0.27836019477125973
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2314	96-97	-0.8984717901340922
2314	98-99	-0.7966762094447972
2314	100	-0.35567396983523025
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2315	3	-0.24988700131813602
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2315	5	-0.19495996124251747
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2315	10-11	-0.3515759510350307
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2315	14-15	-0.2322987621023742
2315	16-17	-0.20623258858528715
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2315	22-23	-0.13885977100517266
2315	24-25	-0.09906573781298533
2315	26-27	-0.2651779101470382
2315	28-29	-0.24491565671892346
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2315	100	0.19339187094759325
2316	1	-0.4901119144415098
2316	2	-0.8171247212828696
2316	3	-0.5019261122407777
2316	4	-0.2704699963895649
2316	5	-0.30962586674392156
2316	6	-0.30081420854077123
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2316	12-13	-0.5079300835867784
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2316	56-57	-0.3923235975646051
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2316	60-61	-0.47494321590200883
2316	62-63	-0.5101359640575964
2316	64-65	-0.29866162841176447
2316	66-67	-0.2919729867384504
2316	68-69	-0.18885717338567787
2316	70-71	-0.25516214968789086
2316	72-73	-0.467044210377054
2316	74-75	-0.18781052173849844
2316	76-77	-0.21426714584463014
2316	78-79	-0.4835122128215872
2316	80-81	-0.18234100690479949
2316	82-83	-0.038147977284143764
2316	84-85	-0.05056238282894654
2316	86-87	-0.008391239249451843
2316	88-89	-0.15332314328712116
2316	90-91	0.023475088301147196
2316	92-93	0.020903889788240093
2316	94-95	-0.0680546272779381
2316	96-97	-0.10760021701564426
2316	98-99	0.06601834899009873
2316	100	-0.23149124553708944
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	14.0
1	50.0
2	91.0
3	99.0
4	118.0
5	119.0
6	150.0
7	661.0
8	1533.0
9	2796.0
10	5157.0
11	9259.0
12	14115.0
13	17429.0
14	19153.0
15	20543.0
16	22665.0
17	26223.0
18	30691.0
19	35823.0
20	42432.0
21	50926.0
22	59247.0
23	63504.0
24	65736.0
25	67842.0
26	73574.0
27	83661.0
28	96993.0
29	115225.0
30	140449.0
31	173493.0
32	219636.0
33	287771.0
34	397528.0
35	570397.0
36	882104.0
37	1376191.0
38	1387292.0
39	332217.0
40	317.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.46690392779752	18.576011226682347	12.96202659692987	23.99505824859027
2	15.907450908371414	20.14386541772732	40.127572953792004	23.82111072010926
3	18.46920655625202	25.5905238802486	30.099613495929468	25.840656067569917
4	12.555752898014866	16.4816954739497	37.93277430485604	33.0297773231794
5	13.157127062972116	37.61145212733048	35.182796283848106	14.048624525849302
6	31.125327148226667	36.947321758470366	17.940997751852773	13.986353341450188
7	26.99001254744137	32.3361220188621	22.853241551842686	17.82062388185384
8	25.634860277100024	35.5290614368382	20.572293882015096	18.26378440404668
9	26.389767727204337	15.249736921254776	20.337514752655178	38.02298059888571
10-11	24.74666204089475	25.836542313187767	28.997859982844602	20.41893566307288
12-13	25.24381751837414	23.692783614058264	28.85222340583928	22.211175461728317
14-15	22.67287339358951	25.10461598417476	26.190016159366557	26.03249446286917
16-17	22.324503891114535	27.95868593228068	26.935180265495877	22.781629911108904
18-19	22.238162100266422	27.201110911789993	28.610730421065067	21.949996566878514
20-21	22.90711135558362	26.45560888821943	28.607064089902522	22.030215666294428
22-23	22.758264845751977	26.253956495588216	28.44040982538208	22.54736883327773
24-25	22.485245828150042	26.448068079510772	28.45701136028272	22.60967473205647
26-27	22.34292861631828	26.56553647210633	28.467609121366095	22.6239257902093
28-29	22.359602312907196	26.61422146211936	28.52956410917027	22.49661211580317
30-31	22.667006063493154	26.50584865556345	28.487367766172422	22.339777514770972
32-33	22.437883186678597	26.653766011128667	28.38507343496055	22.523277367232186
34-35	22.641140498975027	26.722894529756818	28.335909590700847	22.300055380567308
36-37	22.670202195825716	26.64916684835455	28.263407034108056	22.417223921711674
38-39	22.529244611702147	26.765615132810776	28.25135138931519	22.453788866171887
40-41	22.576971295004313	26.633122385789964	28.342568608512853	22.447337710692867
42-43	22.471146800936932	26.72281686583398	28.207800573383658	22.598235759845434
44-45	22.466649289732775	26.807577259850824	28.157089475167773	22.56868397524863
46-47	22.499680023060755	26.7207231561016	27.93092712169367	22.84866969914397
48-49	22.5071897051894	26.845144736593397	27.938903363473827	22.708762194743382
50-51	22.464751156109482	27.02505749355733	27.820195147126874	22.689996203206313
52-53	22.5306078045213	27.08464743398638	27.698170861552406	22.686573899939912
54-55	22.400512346973255	27.050116422794744	27.809132371096183	22.740238859135818
56-57	22.506477903400786	27.101395999390316	27.62225828529075	22.769867811918147
58-59	22.52209304671464	26.99196827059658	27.770363822621995	22.715574860066788
60-61	22.60547051177404	27.05514058998868	27.788404721989675	22.550984176247603
62-63	22.44657191243491	27.113403055813883	27.81540122364994	22.62462380810127
64-65	22.4162562010718	26.94718959968508	27.84142622334888	22.795127975894243
66-67	22.427117358426536	26.98237445223935	27.730053868812103	22.86045432052201
68-69	22.385937724164314	27.037832958729858	27.749905850987517	22.82632346611831
70-71	22.389073825631755	26.909506745009697	27.76730284507642	22.93411658428213
72-73	22.356318983011203	27.134764392389027	27.549793688508693	22.959122936091077
74-75	22.371610999167718	27.166777256593118	27.551142952307877	22.910468791931287
76-77	22.37179272124553	27.05188544082484	27.595701030987147	22.980620806942476
78-79	22.32378513146304	27.036364057478984	27.593386913242835	23.04646389781514
80-81	22.38459644058724	27.173839299510533	27.403770716840874	23.03779354306135
82-83	22.464413808479357	27.13369212658221	27.449633610686814	22.95226045425162
84-85	22.399250038235525	27.130518346739862	27.446145436665415	23.024086178359195
86-87	22.280608016887314	27.125437647515387	27.565238890408555	23.028715445188737
88-89	22.257345592218794	27.013584846847237	27.567751082970577	23.16131847796339
90-91	22.47965188786155	27.016784362391945	27.50988693409323	22.99367681565327
92-93	22.381161103585807	26.998133152885394	27.493179402632833	23.12752634089597
94-95	22.322382409782982	26.89949576707646	27.510312021299633	23.26780980184093
96-97	22.34589740243022	26.86074543454569	27.56513909946302	23.22821806356107
98-99	22.298321606239522	26.83571095447092	27.47669511882228	23.389272320467278
100	22.41566679864222	26.83529346176961	27.448135325910116	23.300904413678047
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1434.0
1	1125.5
2	1365.0
3	2045.0
4	1741.5
5	1508.0
6	2337.0
7	4606.0
8	7366.0
9	8567.0
10	7336.5
11	5784.5
12	5547.0
13	5717.0
14	6089.5
15	6491.0
16	6799.0
17	7168.0
18	7740.5
19	8381.5
20	9280.5
21	10782.0
22	13012.0
23	16229.0
24	20506.0
25	25748.0
26	32601.5
27	41128.5
28	50511.0
29	61349.0
30	74384.0
31	86990.0
32	99553.5
33	113486.5
34	127404.0
35	139789.0
36	156190.0
37	172806.5
38	183558.5
39	192593.5
40	199238.0
41	206175.5
42	214844.0
43	223806.0
44	231015.5
45	238837.0
46	246966.5
47	251547.0
48	256626.5
49	260462.0
50	260251.5
51	254520.0
52	244867.5
53	237174.5
54	228170.0
55	213256.0
56	194561.5
57	174726.0
58	155278.5
59	135346.5
60	114419.0
61	94883.5
62	77488.5
63	60974.5
64	45759.0
65	34442.0
66	26017.5
67	19853.0
68	15218.5
69	11589.5
70	9037.5
71	7037.5
72	5484.5
73	4223.0
74	3324.5
75	2584.0
76	1893.5
77	1285.5
78	933.5
79	668.0
80	447.5
81	333.5
82	252.5
83	212.0
84	166.0
85	125.5
86	105.5
87	96.5
88	78.0
89	56.0
90	39.0
91	32.5
92	38.0
93	29.5
94	23.5
95	20.0
96	12.0
97	9.0
98	5.0
99	6.5
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1579657277270266
2	0.10005641526415372
3	0.029313227825633805
4	0.03125549062753615
5	0.026683702801519864
6	0.0023456558453743667
7	0.003376549178691763
8	0.004990121352579863
9	0.005393514396051888
10-11	0.004287918647276709
12-13	0.0069099734298448704
14-15	0.01688274589345882
16-17	0.008067860869440497
18-19	0.01588920376787031
20-21	0.018899711110818944
22-23	0.04422382995100717
24-25	0.015672566763042742
26-27	0.009412504347680579
28-29	0.02587691671457582
30-31	0.014335393526348439
32-33	0.01966167574848832
34-35	0.020109890241235016
36-37	0.02009494975814346
38-39	0.018257270337882013
40-41	0.02496554724599087
42-43	0.033489092849723845
44-45	0.035289421062256396
46-47	0.019953015168773673
48-49	0.012587357004636332
50-51	0.01098125507229401
52-53	0.02069256908180572
54-55	0.024875904347441528
56-57	0.018585960965896254
58-59	0.024487451787061063
60-61	0.024472511303969505
62-63	0.04960987410551328
64-65	0.030508466472958323
66-67	0.019699026956217214
68-69	0.025458583188012234
70-71	0.03956239922644155
72-73	0.03722421362261296
74-75	0.03870332144867705
76-77	0.021417182511746208
78-79	0.015523161932127178
80-81	0.021603938550390663
82-83	0.017360841352388627
84-85	0.017174085313744168
86-87	0.019982896134956785
88-89	0.01641212067607479
90-91	0.02714685777735812
92-93	0.02581715478220959
94-95	0.01907899690791762
96-97	0.013035571497383025
98-99	0.01749530570021263
100	0.038710791690222825
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6693224.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.730169188857985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.54824783484591	43.93049286719405
2	15.628815431507148	18.67023579890713
3	5.160733640640114	9.24754480482195
4	2.1362814381356037	5.104018069394259
5	1.1203546799809967	3.3459487293396886
6	0.6267961148753862	2.246318279305541
7	0.3860091488229058	1.6139474237347458
8	0.27194149562230774	1.299448923439322
9	0.20455779310909428	1.0996444432175048
>10	0.8620280660378452	8.728873979079825
>50	0.03796727895229342	1.544102583135443
>100	0.015285640472378903	1.6067619470958325
>500	5.076407336778525E-4	0.20115246941133716
>1k	3.7226849344318023E-4	0.39316689898070184
>5k	0.0	0.0
>10k+	1.0152777093411468E-4	0.9683427829426821
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19530	0.29178763477809794	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17353	0.25926220308777953	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11021	0.16465906415204393	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	1.4940483091556476E-5
10-11	0.0	0.0	0.0	0.0	2.9880966183112952E-5
12-13	0.0	7.470241545778238E-6	0.0	0.0	2.9880966183112952E-5
14-15	0.0	5.2291690820447665E-5	0.0	1.4940483091556476E-5	5.2291690820447665E-5
16-17	0.0	8.964289854933885E-5	0.0	1.4940483091556476E-5	5.9761932366225905E-5
18-19	0.0	8.964289854933885E-5	0.0	1.4940483091556476E-5	6.723217391200415E-5
20-21	0.0	8.964289854933885E-5	0.0	1.4940483091556476E-5	7.470241545778238E-5
22-23	0.0	8.964289854933885E-5	0.0	6.723217391200414E-5	9.71131400951171E-5
24-25	0.0	9.711314009511708E-5	0.0	1.56875072461343E-4	1.1952386473245181E-4
26-27	0.0	1.0458338164089533E-4	0.0	2.3904772946490362E-4	1.2699410627823004E-4
28-29	0.0	1.0458338164089533E-4	0.0	7.843753623067149E-4	1.419345893697865E-4
30-31	0.0	1.419345893697865E-4	0.0	0.002786400096575283	1.4940483091556477E-4
32-33	0.0	1.6434531400712123E-4	0.0	0.007978217970891158	1.4940483091556477E-4
34-35	0.0	1.6434531400712123E-4	0.0	0.0167856327533637	1.6434531400712123E-4
36-37	0.0	1.7181555555289947E-4	0.0	0.03216686009612109	1.8675603864445596E-4
38-39	7.470241545778238E-6	1.9422628019023417E-4	0.0	0.05616874618270657	1.942262801902342E-4
40-41	1.4940483091556476E-5	2.0916676328179066E-4	0.0	0.08593018850108708	2.0916676328179066E-4
42-43	1.4940483091556476E-5	2.3904772946490362E-4	0.0	0.1151612436697173	2.0916676328179066E-4
44-45	1.4940483091556476E-5	2.3904772946490362E-4	0.0	0.15189989159185469	2.0916676328179066E-4
46-47	1.4940483091556476E-5	2.3904772946490362E-4	0.0	0.19885035970707093	2.0916676328179066E-4
48-49	1.4940483091556476E-5	2.465179710106818E-4	0.0	0.24993934163864828	2.2410724637334713E-4
50-51	1.4940483091556476E-5	2.6145845410223835E-4	0.0	0.30654136183101	2.2410724637334713E-4
52-53	1.4940483091556476E-5	2.6892869564801655E-4	0.0	0.36285951284463214	2.6145845410223835E-4
54-55	1.4940483091556476E-5	2.913394202853513E-4	0.0	0.42878439448612504	3.13750144922686E-4
56-57	1.4940483091556476E-5	2.9880966183112954E-4	0.0	0.5083872883979379	3.3616086956002073E-4
58-59	1.4940483091556476E-5	2.9880966183112954E-4	0.0	0.5987473301356716	3.4363111110579894E-4
60-61	1.4940483091556476E-5	3.13750144922686E-4	0.0	0.6971976434674829	3.5110135265157714E-4
62-63	1.4940483091556476E-5	3.2869062801424247E-4	0.0	0.7989124523547995	3.7351207728891187E-4
64-65	1.4940483091556476E-5	3.2869062801424247E-4	0.0	0.9081049730294399	3.7351207728891187E-4
66-67	1.4940483091556476E-5	3.2869062801424247E-4	0.0	1.0356219962158746	3.7351207728891187E-4
68-69	1.4940483091556476E-5	3.2869062801424247E-4	0.0	1.1886050728318671	3.7351207728891187E-4
70-71	1.4940483091556476E-5	3.2869062801424247E-4	0.0	1.3586651216215087	4.108632850178031E-4
72-73	1.4940483091556476E-5	3.5110135265157714E-4	0.0	1.5314816895415424	4.631549758382507E-4
74-75	1.4940483091556476E-5	3.7351207728891187E-4	0.0	1.7044252515678542	4.631549758382507E-4
76-77	1.4940483091556476E-5	3.884525603804684E-4	0.0	1.895842720936876	5.005061835671419E-4
78-79	1.4940483091556476E-5	3.884525603804684E-4	0.0	2.1178807104020425	5.378573912960331E-4
80-81	1.4940483091556476E-5	3.884525603804684E-4	0.0	2.364854366147017	5.527978743875896E-4
82-83	1.4940483091556476E-5	4.0339304347202485E-4	0.0	2.625431331746853	5.527978743875896E-4
84-85	1.4940483091556476E-5	4.0339304347202485E-4	0.0	2.8879580303901378	5.752085990249243E-4
86-87	1.4940483091556476E-5	4.0339304347202485E-4	0.0	3.158746816183053	5.826788405707026E-4
88	1.4940483091556476E-5	4.0339304347202485E-4	0.0	3.376250369029932	5.826788405707026E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10815	0.0	49.84555	1
GTATCAA	17885	0.0	36.327675	1
TCAACGC	22005	0.0	28.976652	4
ATCAACG	22250	0.0	28.637964	3
CAACGCA	22375	0.0	28.453358	5
TATCAAC	23025	0.0	27.87709	2
AACGCAG	23125	0.0	27.585522	6
GTACATG	22550	0.0	27.058636	1
TACCTGG	5390	0.0	26.259844	2
TACATGG	22850	0.0	25.806303	2
ACATGGG	23125	0.0	24.75016	3
ACGCAGA	25860	0.0	24.524174	7
GTACCTG	6100	0.0	24.080872	1
CGCAGAG	26655	0.0	23.634813	8
CATGGGG	14945	0.0	23.171883	4
GCAGAGT	29820	0.0	20.65552	9
GAGTACT	17185	0.0	19.958996	12-13
ACCTGGG	7090	0.0	19.949533	3
ATGGGGG	9215	0.0	19.885044	5
AGAGTAC	27130	0.0	19.30957	10-11
>>END_MODULE