FastQCFastQC Report
Mon 6 Jun 2016
ERR1211474_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211474_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6693224
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222140.3318878913958356No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203040.3033515686909627No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139000.20767271497263504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115300.048.905161
GTATCAA190000.036.311261
TCAACGC237400.028.7223834
CAACGCA240800.028.3751685
ATCAACG242100.028.1649913
TATCAAC249250.027.5655652
AACGCAG251600.027.324036
TACCTGG54300.026.2418612
GTACATG222950.025.3759881
TACATGG224600.024.8119242
ACGCAGA283550.024.1291927
ACATGGG230650.023.3489933
GTACCTG61800.023.2861541
CGCAGAG291850.023.2819658
CATGGGG148900.021.9343034
GCAGAGT324050.020.663829
GAGTACT194550.020.10897812-13
AGAGTAC297400.019.4587210-11
ACCTGGG71350.019.1003363
CAGAGTA317600.018.70197110-11
Produced by FastQC (version 0.11.5)