##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211474_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6693224 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.066298543123615 33.0 31.0 34.0 30.0 34.0 2 32.30218890029678 34.0 31.0 34.0 30.0 34.0 3 32.314451750008665 34.0 31.0 34.0 30.0 34.0 4 35.96516940117348 37.0 35.0 37.0 35.0 37.0 5 35.8809364515516 37.0 35.0 37.0 35.0 37.0 6 35.89844953642669 37.0 35.0 37.0 35.0 37.0 7 35.82212592914864 37.0 35.0 37.0 35.0 37.0 8 35.816598099809596 37.0 35.0 37.0 35.0 37.0 9 37.46059238417838 39.0 37.0 39.0 34.0 39.0 10-11 37.41893996077227 39.0 37.0 39.0 34.0 39.0 12-13 37.330785881363 39.0 37.0 39.0 34.0 39.0 14-15 38.550070339794395 40.0 38.0 41.0 34.0 41.0 16-17 38.472429579526995 40.0 38.0 41.0 34.0 41.0 18-19 38.45025043237759 40.0 38.0 41.0 34.0 41.0 20-21 38.38410024825107 40.0 38.0 41.0 34.0 41.0 22-23 38.275774947917476 40.0 38.0 41.0 34.0 41.0 24-25 38.15942108018498 40.0 37.5 41.0 33.0 41.0 26-27 37.975412671083475 40.0 37.0 41.0 33.0 41.0 28-29 38.01546788214469 40.0 37.0 41.0 33.0 41.0 30-31 37.93706575485895 40.0 37.0 41.0 33.0 41.0 32-33 37.75934437574479 40.0 37.0 41.0 33.0 41.0 34-35 37.646448632228655 40.0 37.0 41.0 33.0 41.0 36-37 37.59007706898798 40.0 37.0 41.0 32.0 41.0 38-39 37.490573750407876 40.0 36.5 41.0 32.0 41.0 40-41 37.321734563194056 40.0 36.0 41.0 31.0 41.0 42-43 37.16342737072597 39.0 36.0 41.0 31.0 41.0 44-45 36.97010521387003 39.0 36.0 41.0 30.5 41.0 46-47 36.707788279609346 39.0 35.0 41.0 30.0 41.0 48-49 36.476069828232255 39.0 35.0 40.5 30.0 41.0 50-51 36.26672990475143 39.0 35.0 40.0 29.0 41.0 52-53 36.05303722092671 39.0 35.0 40.0 28.0 41.0 54-55 35.84706092310671 38.0 34.5 40.0 28.0 41.0 56-57 35.636531513064554 38.0 34.0 40.0 27.5 41.0 58-59 35.359473327054346 38.0 34.0 40.0 27.0 41.0 60-61 35.03414542827194 37.5 34.0 40.0 26.0 41.0 62-63 34.7020700338133 37.0 33.0 40.0 26.0 41.0 64-65 34.370532795555626 36.5 33.0 39.0 26.0 41.0 66-67 34.015680111707 36.0 32.5 39.0 25.5 40.5 68-69 33.60902055571425 35.5 32.0 39.0 24.5 40.0 70-71 33.21525000507977 35.0 31.5 38.0 24.0 40.0 72-73 32.80465393060206 35.0 31.0 37.5 23.0 39.5 74-75 32.397881349854714 35.0 31.0 37.0 22.5 39.0 76-77 30.88706608354957 33.5 29.5 35.5 21.0 37.5 78-79 31.732684801823456 34.0 30.5 36.0 22.0 38.0 80-81 31.712148883706867 34.0 31.0 36.0 22.0 37.0 82-83 31.434531699521784 34.0 30.5 35.0 21.0 37.0 84-85 31.13619116885973 34.0 30.0 35.0 20.0 36.5 86-87 30.841203357305837 34.0 30.0 35.0 20.0 36.0 88-89 30.559110079088942 34.0 29.5 35.0 19.5 36.0 90-91 30.269502709008393 34.0 29.0 35.0 19.0 35.0 92-93 30.055393185705423 34.0 29.0 35.0 18.0 35.0 94-95 29.828078964636475 34.0 29.0 35.0 18.0 35.0 96-97 29.612140800905514 33.0 29.0 35.0 18.0 35.0 98-99 29.437523232451206 33.0 28.0 35.0 17.0 35.0 100 27.489421092137363 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2582220587660018 1101 2 0.18406896706459008 1101 3 0.2342933172980537 1101 4 0.11675150776112986 1101 5 0.1391328177861979 1101 6 0.14308347932953325 1101 7 0.14413627532852047 1101 8 0.11247614148716423 1101 9 0.17470985039130227 1101 10-11 0.20401387400427495 1101 12-13 0.18019618477363508 1101 14-15 0.24893824789352692 1101 16-17 0.23224971380991377 1101 18-19 0.23319518146323048 1101 20-21 0.19333221268954048 1101 22-23 0.20023586701528728 1101 24-25 0.13572265552071627 1101 26-27 0.09636345535248125 1101 28-29 0.1719711566627069 1101 30-31 0.2845872113812504 1101 32-33 0.3471201611767327 1101 34-35 0.2503630808657107 1101 36-37 0.3597449290777419 1101 38-39 0.25668219631928224 1101 40-41 0.22548324187538782 1101 42-43 0.22569472287907644 1101 44-45 0.3140737792478703 1101 46-47 0.3678155038450228 1101 48-49 0.36057438350223237 1101 50-51 0.3213663654723078 1101 52-53 0.24767047142755416 1101 54-55 0.253060772167089 1101 56-57 0.30760691922558436 1101 58-59 0.35208617573029954 1101 60-61 0.2544121701983073 1101 62-63 0.15564335413648678 1101 64-65 0.1989590910208321 1101 66-67 0.061029780920158316 1101 68-69 0.04995114734163053 1101 70-71 0.11126741381610117 1101 72-73 -0.01674391621397575 1101 74-75 0.00162746158906657 1101 76-77 0.0012133623010939232 1101 78-79 0.16091959375106413 1101 80-81 0.16135392012094485 1101 82-83 0.03213770097038804 1101 84-85 0.07638622433862707 1101 86-87 0.0035337537589761325 1101 88-89 -0.022743963218022856 1101 90-91 -0.12298034656382839 1101 92-93 -0.1605255260775742 1101 94-95 -0.16896169380986237 1101 96-97 -0.28732919105662447 1101 98-99 -0.32713565729767424 1101 100 -0.1547640114166562 1102 1 0.27480398355186253 1102 2 0.18551934814955473 1102 3 0.27917231336917325 1102 4 0.19478217819516175 1102 5 0.14138280384166535 1102 6 0.13176378435885283 1102 7 0.19452894168487234 1102 8 0.14383647289783852 1102 9 0.18910430844540116 1102 10-11 0.1874842447279974 1102 12-13 0.2218983009985891 1102 14-15 0.32769565878160023 1102 16-17 0.3281840194457075 1102 18-19 0.24219485491741466 1102 20-21 0.26602019796007426 1102 22-23 0.20906803798030893 1102 24-25 0.19441610660101816 1102 26-27 0.21264260024872073 1102 28-29 0.2688023382239564 1102 30-31 0.32949134216255516 1102 32-33 0.363717370331301 1102 34-35 0.31686829156563334 1102 36-37 0.34639062039926216 1102 38-39 0.35025948797134987 1102 40-41 0.3777019225185967 1102 42-43 0.2614400621783446 1102 44-45 0.338217806515253 1102 46-47 0.32731412090375755 1102 48-49 0.31472761705748553 1102 50-51 0.42026140365082654 1102 52-53 0.3937720806783105 1102 54-55 0.44041450043170727 1102 56-57 0.3911388699892697 1102 58-59 0.3872542289982519 1102 60-61 0.39458007379709414 1102 62-63 0.3488664853973944 1102 64-65 0.38000782578601644 1102 66-67 0.4179689207300825 1102 68-69 0.3485823905744212 1102 70-71 0.3961809221224968 1102 72-73 0.3957788144993728 1102 74-75 0.3129979320355858 1102 76-77 0.37982679200830205 1102 78-79 0.29353798089704597 1102 80-81 0.35855702816790824 1102 82-83 0.32453029812225154 1102 84-85 0.2898889398490212 1102 86-87 0.25281894108810476 1102 88-89 0.2126937074344788 1102 90-91 0.20705789348882675 1102 92-93 0.1410686625234696 1102 94-95 0.2672730216580277 1102 96-97 0.21551216094951897 1102 98-99 0.07213238856992632 1102 100 0.2703187636637452 1103 1 0.26148163772024446 1103 2 0.2251459656972088 1103 3 0.30170408258483405 1103 4 0.1072294152029798 1103 5 0.15368159423665162 1103 6 0.16160276852333055 1103 7 0.18920405703712362 1103 8 0.1928609765831979 1103 9 0.2147400580940726 1103 10-11 0.16940397023306275 1103 12-13 0.24185942369685876 1103 14-15 0.32486306247425034 1103 16-17 0.28302490587461904 1103 18-19 0.3100120794164667 1103 20-21 0.27130602949710436 1103 22-23 0.30930354363523094 1103 24-25 0.305661622002134 1103 26-27 0.32397523628106484 1103 28-29 0.38942373564447763 1103 30-31 0.4650766878229007 1103 32-33 0.4676519754354871 1103 34-35 0.42656282457245 1103 36-37 0.48818966591324653 1103 38-39 0.4828263221212694 1103 40-41 0.532977034746601 1103 42-43 0.5444649263281036 1103 44-45 0.48257901604300457 1103 46-47 0.5089677197556952 1103 48-49 0.593397961379381 1103 50-51 0.5228079296232764 1103 52-53 0.5004979893742103 1103 54-55 0.6204720168453548 1103 56-57 0.6176953996049903 1103 58-59 0.5386965093684566 1103 60-61 0.5968620856319475 1103 62-63 0.6632805322442223 1103 64-65 0.6908865442930434 1103 66-67 0.6282928219113231 1103 68-69 0.5796718525446138 1103 70-71 0.6479712202995742 1103 72-73 0.6229196570376203 1103 74-75 0.541519966920454 1103 76-77 0.5591755574512902 1103 78-79 0.6532949288933629 1103 80-81 0.6154875835624978 1103 82-83 0.5549583207756292 1103 84-85 0.5869209370284629 1103 86-87 0.6132964819660707 1103 88-89 0.6180925998266709 1103 90-91 0.6440846264769107 1103 92-93 0.68777279554077 1103 94-95 0.7061107551711316 1103 96-97 0.7032973277438153 1103 98-99 0.6684171751632917 1103 100 0.8250108395440243 1104 1 0.1986992372343508 1104 2 0.20024768663039794 1104 3 0.2239270465982912 1104 4 0.04084556206505141 1104 5 0.13245422645587723 1104 6 0.15846611551685896 1104 7 0.14139549941871365 1104 8 0.13141739688750675 1104 9 0.21372706348510206 1104 10-11 0.2636425900877555 1104 12-13 0.21185031539960875 1104 14-15 0.3480405263619417 1104 16-17 0.2622442425289293 1104 18-19 0.29299165138493777 1104 20-21 0.21355196206468463 1104 22-23 0.28243364527636317 1104 24-25 0.29991417292104217 1104 26-27 0.315395633876669 1104 28-29 0.39626099968462114 1104 30-31 0.29464290179460306 1104 32-33 0.32439674597586077 1104 34-35 0.442349181727856 1104 36-37 0.31723732847285646 1104 38-39 0.3958681584726804 1104 40-41 0.5131410032038346 1104 42-43 0.5421711898110075 1104 44-45 0.4442144162090287 1104 46-47 0.4611430618956618 1104 48-49 0.5015272571654918 1104 50-51 0.4846670191846769 1104 52-53 0.49623988288850285 1104 54-55 0.49307611544423935 1104 56-57 0.5784737499107351 1104 58-59 0.5364335965251072 1104 60-61 0.4821904327794613 1104 62-63 0.5117312619082881 1104 64-65 0.6072654296775326 1104 66-67 0.5456799814150983 1104 68-69 0.6035792502487922 1104 70-71 0.4785861135344618 1104 72-73 0.6033793866524704 1104 74-75 0.5721114094369142 1104 76-77 0.5133775350667271 1104 78-79 0.6503721590268974 1104 80-81 0.7143441067509997 1104 82-83 0.5332503340892139 1104 84-85 0.6342210023735291 1104 86-87 0.6249717421718266 1104 88-89 0.6735260127114948 1104 90-91 0.5068104260435717 1104 92-93 0.49643384325121076 1104 94-95 0.522430066851804 1104 96-97 0.477787509237654 1104 98-99 0.568837798892833 1104 100 0.7255802991895699 1105 1 0.24641422893964204 1105 2 0.13730753894686387 1105 3 0.23750293587873017 1105 4 0.1112716099254456 1105 5 0.11187093247804825 1105 6 0.1312989465362122 1105 7 0.1428981174024031 1105 8 0.1782704517978857 1105 9 0.19189853797232814 1105 10-11 0.19754502450038558 1105 12-13 0.25324149355235903 1105 14-15 0.28457204755395793 1105 16-17 0.2715910276754414 1105 18-19 0.2399032778357082 1105 20-21 0.19610186440148425 1105 22-23 0.2893792317876702 1105 24-25 0.27388351315280346 1105 26-27 0.3045448749237707 1105 28-29 0.3609307887010189 1105 30-31 0.4419967902794326 1105 32-33 0.4189191709900655 1105 34-35 0.4408697226615317 1105 36-37 0.47307188790963295 1105 38-39 0.4245438450979435 1105 40-41 0.47182274632570653 1105 42-43 0.41530370894715674 1105 44-45 0.4596966654851755 1105 46-47 0.5080220606565149 1105 48-49 0.5611713265918254 1105 50-51 0.597469158775489 1105 52-53 0.5672501803985099 1105 54-55 0.5447974899230559 1105 56-57 0.49334226589024155 1105 58-59 0.6061177162209432 1105 60-61 0.5861087823518929 1105 62-63 0.5850916032296922 1105 64-65 0.5980681881097993 1105 66-67 0.5307015851671935 1105 68-69 0.6088310477765404 1105 70-71 0.6246221057167176 1105 72-73 0.6037796474980937 1105 74-75 0.6823224619276331 1105 76-77 0.6507061774001137 1105 78-79 0.7332921130139418 1105 80-81 0.7671902644081143 1105 82-83 0.703490729514531 1105 84-85 0.7710040909698712 1105 86-87 0.7622644368818889 1105 88-89 0.7602517243571718 1105 90-91 0.7768890608283243 1105 92-93 0.7394218580717471 1105 94-95 0.7463465299984904 1105 96-97 0.7250811772085477 1105 98-99 0.8569914556712277 1105 100 1.0857550376183909 1106 1 0.269983610655089 1106 2 0.22193821103928002 1106 3 0.22229953997238283 1106 4 0.13648176480264596 1106 5 0.16779144639441768 1106 6 0.12751267663041688 1106 7 0.188588577063733 1106 8 0.14218095627955307 1106 9 0.16425203801227894 1106 10-11 0.19050024521889952 1106 12-13 0.18130165059213965 1106 14-15 0.26329208394390946 1106 16-17 0.29000180082986304 1106 18-19 0.32218233023093035 1106 20-21 0.32953787740039786 1106 22-23 0.3708515878168299 1106 24-25 0.38728328281997904 1106 26-27 0.3491701735610562 1106 28-29 0.37079203186782905 1106 30-31 0.35391277524549025 1106 32-33 0.4049094995039084 1106 34-35 0.434665526234987 1106 36-37 0.43583377053958827 1106 38-39 0.40995419966533575 1106 40-41 0.4519055547147488 1106 42-43 0.47851498616539345 1106 44-45 0.46970104195545304 1106 46-47 0.54495234062599 1106 48-49 0.5200044291280506 1106 50-51 0.5365740569767397 1106 52-53 0.5723761120478628 1106 54-55 0.4368918582048664 1106 56-57 0.5039736981836072 1106 58-59 0.6031817241147195 1106 60-61 0.7051804245105657 1106 62-63 0.6719507747206208 1106 64-65 0.7641487474182966 1106 66-67 0.6613141150675759 1106 68-69 0.6333076307282468 1106 70-71 0.641596267681301 1106 72-73 0.6439380507975301 1106 74-75 0.5936546976001864 1106 76-77 0.6082974473315659 1106 78-79 0.6475394847492772 1106 80-81 0.6613198746383517 1106 82-83 0.6710298373450421 1106 84-85 0.6691478847758106 1106 86-87 0.5446036199261108 1106 88-89 0.7101463445242722 1106 90-91 0.6917366944188004 1106 92-93 0.7617931718851914 1106 94-95 0.776956878994504 1106 96-97 0.8844252161554671 1106 98-99 0.8725870294983231 1106 100 0.9242692956014196 1107 1 0.21567754677025164 1107 2 0.1459929694717914 1107 3 0.21991124435211162 1107 4 0.12905908051041592 1107 5 0.10719832412880947 1107 6 0.1421299791660502 1107 7 0.1408348959234047 1107 8 0.14862739745186815 1107 9 0.15307473171317554 1107 10-11 0.1846123965102393 1107 12-13 0.19312062787020778 1107 14-15 0.19335785051492138 1107 16-17 0.18637927648494212 1107 18-19 0.15618816360345988 1107 20-21 0.24103432562922222 1107 22-23 0.3029500965433627 1107 24-25 0.3525401864282074 1107 26-27 0.37791458716598214 1107 28-29 0.3588556344458027 1107 30-31 0.3089015318903563 1107 32-33 0.2734309277393123 1107 34-35 0.28446380444922426 1107 36-37 0.38520818837842086 1107 38-39 0.3762513253180657 1107 40-41 0.3851286812142618 1107 42-43 0.3511920337261998 1107 44-45 0.4498689866324952 1107 46-47 0.4713778945611651 1107 48-49 0.4805540833595998 1107 50-51 0.3381321319637962 1107 52-53 0.3844772552007072 1107 54-55 0.44168924259588493 1107 56-57 0.49564608845503955 1107 58-59 0.5195804633535559 1107 60-61 0.5230431520826571 1107 62-63 0.5582512874928796 1107 64-65 0.6236990132130984 1107 66-67 0.5841663519777711 1107 68-69 0.5080992758333807 1107 70-71 0.61354357915517 1107 72-73 0.5621511570684063 1107 74-75 0.6627189117414147 1107 76-77 0.4858301189220988 1107 78-79 0.5856406279021193 1107 80-81 0.6040858340870017 1107 82-83 0.6208881428444109 1107 84-85 0.5468521704228912 1107 86-87 0.514277703757319 1107 88-89 0.5420099188762499 1107 90-91 0.3958988998099642 1107 92-93 0.5566323307505066 1107 94-95 0.688904943161603 1107 96-97 0.7512123451012265 1107 98-99 0.768873147012556 1107 100 0.9175808635555036 1108 1 0.25109747552950523 1108 2 0.1530966042417532 1108 3 0.1864402908077949 1108 4 0.15801325891066398 1108 5 0.10775191320771427 1108 6 0.1153939988598296 1108 7 0.11684056534397058 1108 8 0.15782349599535195 1108 9 0.08242121909685096 1108 10-11 0.08446038517139698 1108 12-13 0.13126551513452966 1108 14-15 0.16112795924097867 1108 16-17 0.20602292356351626 1108 18-19 0.2182937565614509 1108 20-21 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82-83 -0.013598291829069353 2316 84-85 -0.1263851770876201 2316 86-87 -0.028754624755311653 2316 88-89 -0.05818753061508275 2316 90-91 0.0032905921282946338 2316 92-93 0.08287955518242285 2316 94-95 0.17657687521391807 2316 96-97 0.16295015952973202 2316 98-99 -0.016375218774463463 2316 100 -0.22457077362203748 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 9.0 11 23.0 12 75.0 13 198.0 14 650.0 15 1678.0 16 3838.0 17 7639.0 18 13391.0 19 20845.0 20 29829.0 21 41655.0 22 54709.0 23 64464.0 24 71052.0 25 78431.0 26 90977.0 27 108901.0 28 130540.0 29 158519.0 30 190749.0 31 230834.0 32 282925.0 33 355197.0 34 466158.0 35 637488.0 36 928959.0 37 1360951.0 38 1201315.0 39 161212.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.64449064169505 20.268998880777474 14.238001601365239 23.848508876162235 2 15.219829203046723 21.257539326042597 39.524360341175296 23.99827112973539 3 17.365370108661303 26.70097321408962 30.732373520939138 25.201283156309934 4 12.369126130012024 17.583687039092474 37.703014456056096 32.34417237483941 5 12.826740418032308 38.17320445072021 35.221859943025066 13.778195188222412 6 30.99524410847353 37.794710788845705 17.57117552036613 13.638869582314634 7 26.813535599585492 32.83005320007219 22.65834820409417 17.698062996248147 8 25.71858093273473 36.28271382249064 20.125779210020024 17.872926034754602 9 26.370759442684122 15.27408913850784 20.234792679880428 38.12035873892761 10-11 24.82385170435055 26.31260361225024 28.792768626897892 20.070776056501323 12-13 25.322527678738975 24.0533411103528 28.718320199652663 21.90581101125556 14-15 22.666445945929794 25.499355766369092 26.10377300983801 25.730425277863105 16-17 22.28315820856766 28.382470785002734 26.82965893419429 22.504712072235314 18-19 22.250971366828097 27.471259404770194 28.69649040974155 21.581278818660163 20-21 22.9776092654489 26.679768256203683 28.67677830284737 21.665844175500045 22-23 22.792162640903697 26.49906831147441 28.518864750380384 22.189904297241508 24-25 22.5521620899229 26.685268068910894 28.53028354202452 22.232286299141684 26-27 22.47758120968351 26.772999247670793 28.55002309428797 22.199396448357728 28-29 22.448789937558708 26.802625722621293 28.63880432088427 22.10978001893573 30-31 22.735206239954245 26.689815790110632 28.61836158470646 21.956616385228667 32-33 22.560634929409748 26.822510440902864 28.493973765982584 22.122880863704804 34-35 22.71233879810078 26.879512526995697 28.474478166666177 21.933670508237345 36-37 22.815358355477237 26.8138633945459 28.36822085978481 22.00255739019205 38-39 22.690523056203794 26.886629409080214 28.374198721719807 22.048648812996184 40-41 22.70476035371891 26.823619410762323 28.398328971736404 22.07329126378236 42-43 22.591869813258633 26.871350734955517 28.296571861105374 22.240207590680473 44-45 22.609138496107782 26.98235064243016 28.187380176310544 22.22113068515151 46-47 22.59435440902295 26.936252969236484 28.028195382558984 22.44119723918158 48-49 22.647223226221737 27.013704680503782 27.992939148266082 22.3461329450084 50-51 22.62616224554464 27.21449626880864 27.80060488279658 22.358736602850136 52-53 22.673999038991372 27.258063985962583 27.65713208250809 22.410804892537957 54-55 22.553240825820346 27.22241545668525 27.7544501946638 22.469893522830603 56-57 22.66093885167793 27.266000504999948 27.58249204027449 22.49056860304763 58-59 22.663772112097252 27.16819107645979 27.760068220248606 22.407968591194344 60-61 22.751814792546472 27.234222391426215 27.754694016354346 22.25926879967296 62-63 22.579027995108415 27.273183019560353 27.762722359626984 22.38506662570424 64-65 22.497206027808243 27.124733676180117 27.795555940702048 22.58250435530959 66-67 22.554772980640198 27.14121118331214 27.67173162991427 22.63228420613339 68-69 22.460128689144085 27.21926912383977 27.71068431891311 22.60991786810304 70-71 22.46501067891195 27.099603928871403 27.688484906348382 22.746900485868267 72-73 22.44766031404297 27.316655426886406 27.46545831371321 22.77022594535741 74-75 22.432131554326283 27.374831578321423 27.426213450366667 22.76682341698563 76-77 22.391085009041483 27.289157499558126 27.501348041943864 22.818409449456535 78-79 22.323964301320213 27.26230009963781 27.50342703411256 22.91030856492942 80-81 22.359668102849657 27.451260907805207 27.28225235368486 22.906818635660276 82-83 22.407813008406464 27.41159914810923 27.328718738197498 22.85186910528681 84-85 22.2974183059789 27.407526786036495 27.365364877583648 22.929690030400955 86-87 22.2068883481602 27.40006303243391 27.42748468160925 22.96556393779664 88-89 22.159727579578927 27.301599517324217 27.392568212511836 23.14610469058502 90-91 22.317403641888557 27.303651086564322 27.373349728853004 23.005595542694117 92-93 22.232353567785886 27.320206208170056 27.273651172458308 23.173789051585743 94-95 22.11283431523386 27.24851683648778 27.287966729071083 23.350682119207278 96-97 22.079724161758634 27.248657600375864 27.316569601843945 23.35504863602156 98-99 21.983261822039573 27.157037366559344 27.28144587325903 23.578254938142052 100 21.140749148694667 27.739224718906357 27.42099760971035 23.699028522688625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1487.0 1 1163.5 2 1404.0 3 2060.0 4 1689.0 5 1516.0 6 2366.5 7 4826.0 8 7807.5 9 9275.0 10 8201.0 11 6445.0 12 6347.5 13 6799.0 14 7247.5 15 7634.5 16 8097.0 17 8525.0 18 8962.0 19 9574.5 20 10400.5 21 11872.5 22 14170.0 23 17533.5 24 21957.0 25 27247.5 26 34026.0 27 42384.5 28 52128.0 29 63399.0 30 76346.0 31 88869.0 32 101972.5 33 116091.5 34 129529.5 35 142182.5 36 158581.0 37 174861.5 38 184900.0 39 194111.0 40 202080.0 41 208893.0 42 216356.5 43 224155.5 44 231145.5 45 239450.5 46 247008.5 47 250880.0 48 256471.5 49 260147.5 50 259604.0 51 253595.0 52 242647.0 53 234593.0 54 225239.5 55 209347.5 56 190545.5 57 171244.0 58 151947.5 59 131360.5 60 109924.5 61 90894.0 62 74047.5 63 58070.0 64 43782.0 65 32903.5 66 24787.5 67 18770.5 68 14347.0 69 10930.5 70 8494.5 71 6502.5 72 5013.5 73 3896.5 74 2957.0 75 2239.0 76 1695.0 77 1225.0 78 851.0 79 596.0 80 390.5 81 249.5 82 187.5 83 136.0 84 92.0 85 78.5 86 59.0 87 35.5 88 26.0 89 17.5 90 11.0 91 9.0 92 6.5 93 5.0 94 5.5 95 5.5 96 4.5 97 6.0 98 6.5 99 3.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002763989371937948 2 0.06667937603761655 3 6.125598067538155E-4 4 6.424407729369284E-4 5 0.00156875072461343 6 7.769051207609367E-4 7 0.0 8 0.0010010123671342838 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.499110144827067E-4 18-19 0.0011653576811414051 20-21 0.002136489082092576 22-23 0.0 24-25 1.4940483091556476E-5 26-27 1.56875072461343E-4 28-29 8.516075362187192E-4 30-31 0.0014641673429725346 32-33 0.0029358049274908473 34-35 0.003862114879167349 36-37 0.0010831850241378445 38-39 0.001352113719785861 40-41 0.005064823768037645 42-43 0.006543931594101736 44-45 0.004743603381569181 46-47 0.0063646457970030585 48-49 0.008172444251081393 50-51 0.007806402415338258 52-53 0.003824763671438458 54-55 0.0021813105313672454 56-57 0.002300834396099697 58-59 0.0029358049274908473 60-61 0.006693336425017301 62-63 0.013625720579499507 64-65 0.0036454778743397797 66-67 0.009636611594053927 68-69 0.012221315168893198 70-71 0.00538604415450611 72-73 0.007134080676218217 74-75 0.003017977584494408 76-77 0.002734108405754835 78-79 0.007223723574767556 80-81 0.005490627536147005 82-83 0.015709917970771637 84-85 0.017398192560117515 86-87 0.021379831304017317 88-89 0.020341467729154142 90-91 0.023284742898190765 92-93 0.033145461738618036 94-95 0.036723707439045815 96-97 0.022052153043137356 98-99 0.01729360917847662 100 0.017151674589106835 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6693224.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.51230833679645 #Duplication Level Percentage of deduplicated Percentage of total 1 72.42012979957912 42.37468964623793 2 16.33873243921588 19.12033900631636 3 5.3329348843620465 9.361269908815498 4 2.249503948172732 5.2649467448129545 5 1.1256659807534546 3.293265747504425 6 0.6616023861402993 2.322712968852088 7 0.4202288362722278 1.7211991467981618 8 0.2778732224595245 1.3007202936872744 9 0.20848255829501072 1.0978916160421626 >10 0.904929216520619 8.946908591839664 >50 0.04147548880403525 1.6350694157617414 >100 0.01733322937433086 1.7598891419537563 >500 6.925069222829929E-4 0.29379136719638055 >1k 3.1162734632625606E-4 0.3642755066989624 >5k 0.0 0.0 >10k+ 1.0387578210677704E-4 1.1430308974827141 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22214 0.3318878913958356 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20304 0.3033515686909627 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13900 0.20767271497263504 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 1.4940483091556476E-5 0.0 0.0 0.0 3 0.0 2.9880966183112952E-5 0.0 0.0 0.0 4 0.0 2.9880966183112952E-5 0.0 0.0 0.0 5 0.0 2.9880966183112952E-5 0.0 0.0 0.0 6 0.0 2.9880966183112952E-5 0.0 0.0 0.0 7 0.0 2.9880966183112952E-5 0.0 0.0 2.9880966183112952E-5 8 0.0 2.9880966183112952E-5 0.0 0.0 5.9761932366225905E-5 9 0.0 2.9880966183112952E-5 0.0 0.0 5.9761932366225905E-5 10-11 0.0 2.9880966183112952E-5 0.0 0.0 5.9761932366225905E-5 12-13 0.0 4.4821449274669425E-5 0.0 0.0 5.9761932366225905E-5 14-15 7.470241545778238E-6 8.217265700356062E-5 0.0 0.0 7.470241545778238E-5 16-17 1.4940483091556476E-5 8.964289854933885E-5 0.0 0.0 7.470241545778238E-5 18-19 1.4940483091556476E-5 8.964289854933885E-5 0.0 0.0 8.217265700356062E-5 20-21 1.4940483091556476E-5 8.964289854933885E-5 0.0 0.0 8.964289854933885E-5 22-23 1.4940483091556476E-5 8.964289854933885E-5 0.0 3.735120772889119E-5 1.3446434782400828E-4 24-25 1.4940483091556476E-5 9.711314009511708E-5 0.0 1.2699410627823004E-4 1.942262801902342E-4 26-27 1.4940483091556476E-5 1.0458338164089533E-4 0.0 2.0916676328179066E-4 1.942262801902342E-4 28-29 1.4940483091556476E-5 1.0458338164089533E-4 0.0 7.470241545778237E-4 2.0169652173601243E-4 30-31 1.4940483091556476E-5 1.0458338164089533E-4 0.0 0.0027415786473006134 2.0916676328179066E-4 32-33 1.4940483091556476E-5 1.0458338164089533E-4 0.0 0.007940866763162267 2.0916676328179066E-4 34-35 1.4940483091556476E-5 1.1952386473245181E-4 0.0 0.016688519613268584 2.0916676328179066E-4 36-37 1.4940483091556476E-5 1.3446434782400828E-4 0.0 0.03204733623138864 2.0916676328179066E-4 38-39 1.4940483091556476E-5 1.419345893697865E-4 0.0 0.05591475797015011 2.2410724637334713E-4 40-41 1.4940483091556476E-5 1.56875072461343E-4 0.0 0.0855417359407066 2.3157748791912536E-4 42-43 1.4940483091556476E-5 1.7181555555289947E-4 0.0 0.11463085651996706 2.3904772946490362E-4 44-45 1.4940483091556476E-5 1.792857970986777E-4 0.0 0.15130974250973822 2.465179710106818E-4 46-47 1.4940483091556476E-5 1.792857970986777E-4 0.0 0.19823032965877133 2.539882125564601E-4 48-49 1.4940483091556476E-5 1.792857970986777E-4 0.0 0.2494537759381727 2.6892869564801655E-4 50-51 1.4940483091556476E-5 1.792857970986777E-4 0.0 0.30561505187933347 2.83869178739573E-4 52-53 1.4940483091556476E-5 1.792857970986777E-4 0.0 0.3616941551634907 3.2869062801424247E-4 54-55 1.4940483091556476E-5 1.792857970986777E-4 0.0 0.4272679354523321 3.8098231883469013E-4 56-57 1.4940483091556476E-5 1.792857970986777E-4 0.0 0.5066168411515886 3.959228019262466E-4 58-59 1.4940483091556476E-5 1.792857970986777E-4 0.0 0.5961925075270154 4.0339304347202485E-4 60-61 1.4940483091556476E-5 2.0916676328179066E-4 0.0 0.693963028878161 4.183335265635813E-4 62-63 1.4940483091556476E-5 2.0916676328179066E-4 0.0 0.7946245337075227 4.631549758382507E-4 64-65 1.4940483091556476E-5 2.0916676328179066E-4 0.0 0.902547113319381 4.7809545892980724E-4 66-67 1.4940483091556476E-5 2.0916676328179066E-4 0.0 1.0288539573753994 4.7809545892980724E-4 68-69 2.9880966183112952E-5 2.0916676328179066E-4 0.0 1.1797304258754826 4.930359420213637E-4 70-71 2.9880966183112952E-5 2.0916676328179066E-4 0.0 1.3470115448100946 5.079764251129202E-4 72-73 2.9880966183112952E-5 2.0916676328179066E-4 0.0 1.517743915338856 5.229169082044767E-4 74-75 2.9880966183112952E-5 2.0916676328179066E-4 0.0 1.688610750215442 5.378573912960332E-4 76-77 2.9880966183112952E-5 2.0916676328179066E-4 0.0 1.8771969980386134 5.67738357479146E-4 78-79 2.9880966183112952E-5 2.0916676328179066E-4 0.0 2.096344004025564 5.67738357479146E-4 80-81 2.9880966183112952E-5 2.0916676328179066E-4 0.0 2.340150277355128 5.826788405707026E-4 82-83 2.9880966183112952E-5 2.0916676328179066E-4 0.0 2.5965812588970576 5.976193236622591E-4 84-85 2.9880966183112952E-5 2.0916676328179066E-4 0.0 2.8550217354148018 6.275002898453719E-4 86-87 2.9880966183112952E-5 2.0916676328179066E-4 0.0 3.1207083462319503 6.424407729369284E-4 88 2.9880966183112952E-5 2.0916676328179066E-4 0.0 3.334551480721398 6.424407729369284E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 11530 0.0 48.90516 1 GTATCAA 19000 0.0 36.31126 1 TCAACGC 23740 0.0 28.722383 4 CAACGCA 24080 0.0 28.375168 5 ATCAACG 24210 0.0 28.164991 3 TATCAAC 24925 0.0 27.565565 2 AACGCAG 25160 0.0 27.32403 6 TACCTGG 5430 0.0 26.241861 2 GTACATG 22295 0.0 25.375988 1 TACATGG 22460 0.0 24.811924 2 ACGCAGA 28355 0.0 24.129192 7 ACATGGG 23065 0.0 23.348993 3 GTACCTG 6180 0.0 23.286154 1 CGCAGAG 29185 0.0 23.281965 8 CATGGGG 14890 0.0 21.934303 4 GCAGAGT 32405 0.0 20.66382 9 GAGTACT 19455 0.0 20.108978 12-13 AGAGTAC 29740 0.0 19.45872 10-11 ACCTGGG 7135 0.0 19.100336 3 CAGAGTA 31760 0.0 18.701971 10-11 >>END_MODULE