FastQCFastQC Report
Mon 6 Jun 2016
ERR1211473_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211473_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8661819
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226170.2611114362930004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192000.2216624475759653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124740.14401132140950995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120000.048.5728721
GTATCAA208300.037.4982951
TCAACGC248500.030.4629884
ATCAACG250150.030.2827633
CAACGCA252250.029.8952435
AACGCAG262000.028.9396156
TATCAAC268200.028.438162
GTACATG275200.027.715391
TACATGG285050.026.0642662
ACATGGG283550.025.2738363
ACGCAGA297650.025.2374617
CGCAGAG306100.024.4033478
CATGGGG155850.023.065244
TACCTGG70000.022.9068722
GTACCTG79750.021.312331
GCAGAGT346950.020.8954739
AGAGTAC317150.019.03675310-11
GAGTACT206250.018.92023312-13
CAGAGTA336650.018.70168110-11
GTATAGG30250.018.365851
Produced by FastQC (version 0.11.5)