FastQCFastQC Report
Mon 6 Jun 2016
ERR1211473_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211473_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8661819
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT253500.29266370031514166No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216470.24991286472275626No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156760.18097815251045998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131150.045.7544141
GTATCAA227600.038.1012571
TCAACGC278200.030.4899854
ATCAACG282350.030.0420133
CAACGCA282800.029.9770725
AACGCAG295500.028.76796
TATCAAC298350.028.63962
GTACATG277400.026.9720731
ACGCAGA332550.025.378827
TACATGG286400.025.302752
CGCAGAG340850.024.7332538
TACCTGG74750.024.724042
ACATGGG283100.024.6505283
GTACCTG81450.023.3837721
CATGGGG157200.022.0916964
GCAGAGT384650.021.3914389
AGAGTAC354950.019.94471410-11
GAGTACT228550.019.42021812-13
ACCTGGG93500.018.847743
AGTACTT248700.018.55534612-13
Produced by FastQC (version 0.11.5)