##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211473_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8661819 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06280920901256 33.0 31.0 34.0 30.0 34.0 2 32.29120788601101 34.0 31.0 34.0 30.0 34.0 3 32.3062441041541 34.0 31.0 34.0 30.0 34.0 4 35.95486352231558 37.0 35.0 37.0 35.0 37.0 5 35.875181991219165 37.0 35.0 37.0 35.0 37.0 6 35.89012781264536 37.0 35.0 37.0 35.0 37.0 7 35.81293213353916 37.0 35.0 37.0 35.0 37.0 8 35.80519126525272 37.0 35.0 37.0 35.0 37.0 9 37.45271507058737 39.0 37.0 39.0 34.0 39.0 10-11 37.411251378030414 39.0 37.0 39.0 34.0 39.0 12-13 37.32470616160416 39.0 37.0 39.0 34.0 39.0 14-15 38.54958121383049 40.0 38.0 41.0 34.0 41.0 16-17 38.47823419076293 40.0 38.0 41.0 34.0 41.0 18-19 38.46035417041155 40.0 38.0 41.0 34.0 41.0 20-21 38.39992367653953 40.0 38.0 41.0 34.0 41.0 22-23 38.29420038677788 40.0 38.0 41.0 34.0 41.0 24-25 38.18651099728591 40.0 38.0 41.0 33.0 41.0 26-27 38.00881858648859 40.0 37.0 41.0 33.0 41.0 28-29 38.046787285672906 40.0 37.5 41.0 33.0 41.0 30-31 37.976854861548134 40.0 37.0 41.0 33.0 41.0 32-33 37.80677517043476 40.0 37.0 41.0 33.0 41.0 34-35 37.69782201636862 40.0 37.0 41.0 33.0 41.0 36-37 37.648139322698846 40.0 37.0 41.0 32.5 41.0 38-39 37.55310114422848 40.0 37.0 41.0 32.0 41.0 40-41 37.39659885527509 40.0 36.5 41.0 31.5 41.0 42-43 37.24713290591734 40.0 36.0 41.0 31.0 41.0 44-45 37.06489202787544 39.0 36.0 41.0 31.0 41.0 46-47 36.81461266969444 39.0 36.0 41.0 30.0 41.0 48-49 36.60352836973389 39.0 35.0 41.0 30.0 41.0 50-51 36.409463878199254 39.0 35.0 40.0 29.5 41.0 52-53 36.21261353995044 39.0 35.0 40.0 28.5 41.0 54-55 36.01619215317245 38.5 35.0 40.0 28.0 41.0 56-57 35.82201140430203 38.0 34.5 40.0 28.0 41.0 58-59 35.56648014695297 38.0 34.0 40.0 27.5 41.0 60-61 35.25334176343329 38.0 34.0 40.0 26.5 41.0 62-63 34.93344279071174 37.5 34.0 40.0 26.0 41.0 64-65 34.61941648746066 37.0 33.0 40.0 26.0 41.0 66-67 34.277475608760696 36.0 33.0 39.0 26.0 41.0 68-69 33.87739399772727 36.0 32.0 39.0 25.0 40.0 70-71 33.49104402897359 35.5 32.0 38.5 24.5 40.0 72-73 33.079524751094425 35.0 31.5 38.0 24.0 40.0 74-75 32.66751856625035 35.0 31.0 37.0 23.0 39.0 76-77 31.125226929816936 33.5 29.5 35.5 21.5 38.5 78-79 31.97308273239143 34.0 31.0 36.0 22.5 39.0 80-81 31.938177015705364 34.0 31.0 36.0 23.0 37.5 82-83 31.646797572195865 34.0 31.0 35.5 22.0 37.0 84-85 31.34122532461138 34.0 30.5 35.0 21.0 37.0 86-87 31.038812055527828 34.0 30.0 35.0 20.0 36.0 88-89 30.752659227813467 34.0 30.0 35.0 20.0 36.0 90-91 30.459943979434343 34.0 29.0 35.0 19.5 35.5 92-93 30.24406749898607 34.0 29.0 35.0 19.0 35.0 94-95 30.015064791817977 34.0 29.0 35.0 18.0 35.0 96-97 29.8074466806568 34.0 29.0 35.0 18.0 35.0 98-99 29.640935581775608 33.5 29.0 35.0 18.0 35.0 100 27.732094378790414 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2967049793643568 1101 2 0.23027162121201172 1101 3 0.2886031385367218 1101 4 0.15868458767845794 1101 5 0.146940449677345 1101 6 0.1888772446959237 1101 7 0.21789662342001037 1101 8 0.16869514412884 1101 9 0.2230816830007214 1101 10-11 0.17753105599791041 1101 12-13 0.20155740177579418 1101 14-15 0.24156648108846923 1101 16-17 0.2587648510094951 1101 18-19 0.3143453838659127 1101 20-21 0.2539384306828012 1101 22-23 0.2785707156173203 1101 24-25 0.26752721581870986 1101 26-27 0.18674749568739912 1101 28-29 0.22587026239414598 1101 30-31 0.334063191789717 1101 32-33 0.3380851852833686 1101 34-35 0.3195311560412293 1101 36-37 0.34784215537663954 1101 38-39 0.3028731546193413 1101 40-41 0.34508433354584866 1101 42-43 0.319289449501305 1101 44-45 0.3467995493014655 1101 46-47 0.31516228412861835 1101 48-49 0.29896800765523324 1101 50-51 0.3217593480708345 1101 52-53 0.26139085803985296 1101 54-55 0.23543955681871154 1101 56-57 0.22653963935211863 1101 58-59 0.25258216625562824 1101 60-61 0.14643130522272685 1101 62-63 0.19708462561957418 1101 64-65 0.2066981476336167 1101 66-67 0.09438052247904949 1101 68-69 0.1365283987325725 1101 70-71 0.11078597944474922 1101 72-73 0.006213269075693972 1101 74-75 0.017051691667397506 1101 76-77 0.10330311575309281 1101 78-79 0.11289631714950232 1101 80-81 0.10728906332643717 1101 82-83 0.08638065403498629 1101 84-85 0.08275250433556636 1101 86-87 0.0011557662155396997 1101 88-89 0.01643842066443213 1101 90-91 0.009676308016480561 1101 92-93 -0.05998150435262417 1101 94-95 -0.027114829923458927 1101 96-97 -0.09441307607173499 1101 98-99 -0.16675877233252123 1101 100 -0.25854991423049967 1102 1 0.304028206155607 1102 2 0.2319466563328021 1102 3 0.2854154054110225 1102 4 0.23128238041203986 1102 5 0.2190139134221738 1102 6 0.1857107299292693 1102 7 0.18749855485205558 1102 8 0.1960142599571313 1102 9 0.23192046845755243 1102 10-11 0.2145111176124601 1102 12-13 0.23960201093979805 1102 14-15 0.3707848787973731 1102 16-17 0.3402721445165966 1102 18-19 0.285127536780422 1102 20-21 0.34137381430003444 1102 22-23 0.3186092321548415 1102 24-25 0.2774162455216498 1102 26-27 0.2701301565971761 1102 28-29 0.34997008856814915 1102 30-31 0.3754249090714197 1102 32-33 0.4421132611407259 1102 34-35 0.3367978403687104 1102 36-37 0.40037868329793014 1102 38-39 0.43188593965393096 1102 40-41 0.4689689165130986 1102 42-43 0.37824193922946137 1102 44-45 0.4087816722586126 1102 46-47 0.4615964765172791 1102 48-49 0.40932614484161434 1102 50-51 0.49173470218863 1102 52-53 0.5049668976751818 1102 54-55 0.4450823159009758 1102 56-57 0.4086159645104246 1102 58-59 0.535356118365776 1102 60-61 0.4651259603783089 1102 62-63 0.5426583011662203 1102 64-65 0.5364905691692172 1102 66-67 0.42244252392222137 1102 68-69 0.4295495761282382 1102 70-71 0.3872097488119195 1102 72-73 0.4571657954117043 1102 74-75 0.4071454098146461 1102 76-77 0.4910725417280801 1102 78-79 0.5057377213798553 1102 80-81 0.4509533446645442 1102 82-83 0.3811036986881895 1102 84-85 0.28917300340836505 1102 86-87 0.26505688595233323 1102 88-89 0.27895779827973755 1102 90-91 0.29035629415582775 1102 92-93 0.1038815427867732 1102 94-95 0.19084198233825234 1102 96-97 0.29547149121563976 1102 98-99 0.26092431739781574 1102 100 0.36451079846353096 1103 1 0.2596216447543327 1103 2 0.1989817354361918 1103 3 0.28637873786385626 1103 4 0.1496595717964908 1103 5 0.1579697444125614 1103 6 0.15398780861973194 1103 7 0.17557548532487743 1103 8 0.18450300801649178 1103 9 0.18435330749591827 1103 10-11 0.206682392866135 1103 12-13 0.2221555696813553 1103 14-15 0.2825282047021105 1103 16-17 0.27419349681409955 1103 18-19 0.31688697167599145 1103 20-21 0.3032091784782196 1103 22-23 0.2621306371811869 1103 24-25 0.2528131414019228 1103 26-27 0.24288675175097296 1103 28-29 0.29502912830100314 1103 30-31 0.37349936693085084 1103 32-33 0.39829430122477305 1103 34-35 0.3933961126135088 1103 36-37 0.3937490874883025 1103 38-39 0.3579844024208896 1103 40-41 0.3958597826071397 1103 42-43 0.4089643977700206 1103 44-45 0.38441921939577384 1103 46-47 0.4428576061239724 1103 48-49 0.40939741670485574 1103 50-51 0.36048981946729697 1103 52-53 0.3514163096119418 1103 54-55 0.4783856951266259 1103 56-57 0.43153624932714507 1103 58-59 0.4543895738559556 1103 60-61 0.4472550980997738 1103 62-63 0.5130091902443681 1103 64-65 0.5413619306512487 1103 66-67 0.5568072545749843 1103 68-69 0.5380090323531732 1103 70-71 0.5594516241674086 1103 72-73 0.4740729140839832 1103 74-75 0.461951290371168 1103 76-77 0.48710114763209234 1103 78-79 0.4948456981103426 1103 80-81 0.39946536147705203 1103 82-83 0.44679668448682364 1103 84-85 0.3049398038959801 1103 86-87 0.345398002862602 1103 88-89 0.39085685328472763 1103 90-91 0.46183725104771867 1103 92-93 0.4188536975798378 1103 94-95 0.5267511443797623 1103 96-97 0.4613480949669828 1103 98-99 0.42657339517437975 1103 100 0.5320313358929134 1104 1 0.21061219308319323 1104 2 0.17452146009843972 1104 3 0.21532568100253968 1104 4 0.04815172091635134 1104 5 0.11851921390592679 1104 6 0.10773620867773559 1104 7 0.15672312554414702 1104 8 0.13447713259478888 1104 9 0.19614681997521188 1104 10-11 0.21212315608558896 1104 12-13 0.21475130640688178 1104 14-15 0.3187368575144447 1104 16-17 0.23577715539963862 1104 18-19 0.2672272119279313 1104 20-21 0.16061523420561485 1104 22-23 0.23002281739145047 1104 24-25 0.25278871140407233 1104 26-27 0.3281717681095202 1104 28-29 0.340802905289884 1104 30-31 0.2844095482077975 1104 32-33 0.3066657631837657 1104 34-35 0.41141128332379395 1104 36-37 0.24343098174831113 1104 38-39 0.34955809076559774 1104 40-41 0.4307050515756785 1104 42-43 0.43048344781963266 1104 44-45 0.44408316408099324 1104 46-47 0.4020377063179481 1104 48-49 0.47442942891655093 1104 50-51 0.42848899924763373 1104 52-53 0.4115194636329207 1104 54-55 0.36654050078524847 1104 56-57 0.45853419865063216 1104 58-59 0.4388989812941517 1104 60-61 0.37531083979875035 1104 62-63 0.4287192187351323 1104 64-65 0.5172476602808302 1104 66-67 0.4728091907637406 1104 68-69 0.38465540917436414 1104 70-71 0.29781092121852026 1104 72-73 0.3941298266399613 1104 74-75 0.4405117121021149 1104 76-77 0.3609006074468475 1104 78-79 0.4805133336567131 1104 80-81 0.5959169499235735 1104 82-83 0.4011657465752698 1104 84-85 0.4581106531226098 1104 86-87 0.5273215852350361 1104 88-89 0.5490173332401618 1104 90-91 0.5018762558421948 1104 92-93 0.513162610721281 1104 94-95 0.5783478676017637 1104 96-97 0.4322317560061286 1104 98-99 0.517920908646321 1104 100 0.5938847581132336 1105 1 0.2373957031023224 1105 2 0.15606769329483683 1105 3 0.20334857435584297 1105 4 0.1470448681688552 1105 5 0.10331554206328519 1105 6 0.09118779881512751 1105 7 0.12986868946973118 1105 8 0.14757972460806457 1105 9 0.15536968289408293 1105 10-11 0.17307938379192223 1105 12-13 0.20550217510169233 1105 14-15 0.29226405771639463 1105 16-17 0.299623315145368 1105 18-19 0.32640110012860646 1105 20-21 0.2378794760086933 1105 22-23 0.2578520331860972 1105 24-25 0.339802917624624 1105 26-27 0.3367882192304279 1105 28-29 0.3973569725075379 1105 30-31 0.39219207447417404 1105 32-33 0.4773915840852254 1105 34-35 0.5265104693221119 1105 36-37 0.5321568632231646 1105 38-39 0.4897166557445445 1105 40-41 0.47849027822478263 1105 42-43 0.4773529255624851 1105 44-45 0.4588662115001654 1105 46-47 0.5501739914121444 1105 48-49 0.5911079064534093 1105 50-51 0.6306638828029989 1105 52-53 0.5904264621606643 1105 54-55 0.4987297483605033 1105 56-57 0.5567609951248329 1105 58-59 0.6246722009844419 1105 60-61 0.6465935285465179 1105 62-63 0.616758253767955 1105 64-65 0.6317373808260953 1105 66-67 0.549608545896092 1105 68-69 0.6742010357243231 1105 70-71 0.6791119364594422 1105 72-73 0.5962047575785832 1105 74-75 0.6347862000423987 1105 76-77 0.6219372458981454 1105 78-79 0.7045900233963884 1105 80-81 0.6504924963187229 1105 82-83 0.6238905014024034 1105 84-85 0.6282419183597838 1105 86-87 0.6495951551885852 1105 88-89 0.5990999872429619 1105 90-91 0.7004778306330977 1105 92-93 0.672998267588131 1105 94-95 0.7381469968440264 1105 96-97 0.702733910522209 1105 98-99 0.7197742839259327 1105 100 0.765718753817783 1106 1 0.23451269616408155 1106 2 0.1757636988223652 1106 3 0.20282195988951202 1106 4 0.1538250267311696 1106 5 0.1541213698668784 1106 6 0.13960929375547693 1106 7 0.15948869131864285 1106 8 0.19138546690006564 1106 9 0.16476225189559557 1106 10-11 0.1951211601358409 1106 12-13 0.19970836080501186 1106 14-15 0.26565992771094926 1106 16-17 0.26966468006029487 1106 18-19 0.2714556314818992 1106 20-21 0.27461339220047876 1106 22-23 0.2814357261357756 1106 24-25 0.28337527312795174 1106 26-27 0.27141479358535037 1106 28-29 0.3181242298660223 1106 30-31 0.3511054921434891 1106 32-33 0.33516521036708724 1106 34-35 0.35444236871507684 1106 36-37 0.3500722184590259 1106 38-39 0.33348223493529616 1106 40-41 0.42351284770844444 1106 42-43 0.3636519955921358 1106 44-45 0.4647726702570196 1106 46-47 0.46252658808229086 1106 48-49 0.4669872777814703 1106 50-51 0.47121491189552245 1106 52-53 0.4763101463223123 1106 54-55 0.4038798451117813 1106 56-57 0.48911474515710296 1106 58-59 0.538377726314728 1106 60-61 0.6295391335897236 1106 62-63 0.5757889457117997 1106 64-65 0.5962592748629092 1106 66-67 0.6200572924836791 1106 68-69 0.5044082197017516 1106 70-71 0.5244378815278381 1106 72-73 0.5124416554461888 1106 74-75 0.5209318347939345 1106 76-77 0.5579486031127203 1106 78-79 0.6402104950745517 1106 80-81 0.687392046811123 1106 82-83 0.6239852134071704 1106 84-85 0.5857903267835773 1106 86-87 0.5460896094138974 1106 88-89 0.625670217316955 1106 90-91 0.6257344790088375 1106 92-93 0.7043146818714128 1106 94-95 0.7517176502983958 1106 96-97 0.751929467356117 1106 98-99 0.7977156959723715 1106 100 0.8218380149932969 1107 1 0.20491993159484423 1107 2 0.17861269864621931 1107 3 0.22598123264183556 1107 4 0.16363689812221338 1107 5 0.1132964920281978 1107 6 0.14562999228169815 1107 7 0.1700873872427806 1107 8 0.1699572510226588 1107 9 0.23071481523553672 1107 10-11 0.1830133667192797 1107 12-13 0.19311325141071478 1107 14-15 0.2734160859402124 1107 16-17 0.2565830572149608 1107 18-19 0.2220752917322244 1107 20-21 0.26855974486066003 1107 22-23 0.3346178462947549 1107 24-25 0.31399136542504635 1107 26-27 0.3485446037702573 1107 28-29 0.3882586969560222 1107 30-31 0.27062265216340364 1107 32-33 0.2838773144988096 1107 34-35 0.3085912147874552 1107 36-37 0.380284296307849 1107 38-39 0.3600557909575599 1107 40-41 0.36888641222962093 1107 42-43 0.3983015610952023 1107 44-45 0.4140035519572933 1107 46-47 0.41049711330922634 1107 48-49 0.44818292758994005 1107 50-51 0.37492952810614355 1107 52-53 0.3994325736777071 1107 54-55 0.43287932188848544 1107 56-57 0.46983138228192445 1107 58-59 0.5596463946411205 1107 60-61 0.5753512219366925 1107 62-63 0.48459928578741795 1107 64-65 0.555259672854973 1107 66-67 0.5972462775365557 1107 68-69 0.5371276250417338 1107 70-71 0.6686974136265817 1107 72-73 0.6560782597633406 1107 74-75 0.651566179789917 1107 76-77 0.560978853587951 1107 78-79 0.6682102563672565 1107 80-81 0.7195815093107534 1107 82-83 0.7122384926533876 1107 84-85 0.5549846447392213 1107 86-87 0.47261075730208546 1107 88-89 0.48509416844665765 1107 90-91 0.40857838892742393 1107 92-93 0.5643870231796804 1107 94-95 0.7565529047824526 1107 96-97 0.7601792946992951 1107 98-99 0.7461447052276071 1107 100 0.8214420822283124 1108 1 0.24291209236044864 1108 2 0.12753194924329847 1108 3 0.1919271993785756 1108 4 0.14867840808231847 1108 5 0.12054280660350969 1108 6 0.1282511214124895 1108 7 0.1502086526876596 1108 8 0.1424548276915445 1108 9 0.09386429447203426 1108 10-11 0.14438264413663404 1108 12-13 0.1469968860609967 1108 14-15 0.17218633623034663 1108 16-17 0.20413924347582935 1108 18-19 0.18018507270198825 1108 20-21 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2316 80-81 -0.07826771807150479 2316 82-83 -0.06127689880472431 2316 84-85 -0.08149721118596176 2316 86-87 -0.06842691752726537 2316 88-89 -0.04748549941453817 2316 90-91 -0.0301681985076101 2316 92-93 0.0802329293420776 2316 94-95 0.13097629535311484 2316 96-97 0.2060070551751103 2316 98-99 0.12758395726148208 2316 100 -0.0747630698460533 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 1.0 9 7.0 10 17.0 11 25.0 12 97.0 13 253.0 14 877.0 15 2139.0 16 4867.0 17 9829.0 18 17300.0 19 26787.0 20 38111.0 21 53457.0 22 68973.0 23 78631.0 24 85516.0 25 95609.0 26 112852.0 27 136494.0 28 164408.0 29 198399.0 30 239560.0 31 289736.0 32 355465.0 33 444973.0 34 580915.0 35 788497.0 36 1152496.0 37 1762271.0 38 1705568.0 39 247657.0 40 31.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.408265914653114 20.468089645890007 13.956677018167426 24.166967421289456 2 14.814867015199221 21.51409491246753 40.078987054450145 23.592051017883104 3 17.243259556043217 26.896292478743312 30.71739363342994 25.14305433178353 4 11.810153110932779 17.807999506797636 38.721915490837375 31.659931891432215 5 12.378923855345684 38.5191564055916 35.84667809937498 13.255241639687737 6 30.524770826074416 38.60611732347298 17.953956712271708 12.915155138180893 7 26.622733631353878 32.83573577328273 23.020072342772345 17.521458252591053 8 25.225384671244726 36.69415650214673 20.495923051088884 17.58453577551965 9 26.131720214726386 15.272528364150972 20.431517000942296 38.16423442018034 10-11 24.238748235214796 26.70163738124752 29.362152453197186 19.6974619303405 12-13 24.968802742241554 24.391250844655147 29.107742842467616 21.532203570635684 14-15 22.207004094636474 25.818468384065753 26.608296709963575 25.3662308113342 16-17 21.821440027781893 28.690456722028607 27.296091733818656 22.192011516370844 18-19 21.67958913036438 27.876968624784983 29.125859506861534 21.3175827379891 20-21 22.49813082687878 27.12976056111677 29.07322725265956 21.298881359344897 22-23 22.217678654892158 27.113723290322927 28.76630351928986 21.90229453549506 24-25 21.967859403187646 27.237359408844657 28.95396208971373 21.84081909825397 26-27 22.002752427192032 27.34966637167442 28.879173572219525 21.768407628914026 28-29 21.936488771776215 27.28784180183575 29.057479648440314 21.71818977794772 30-31 22.195214396456485 27.248952773287698 28.949556824207573 21.606276006048244 32-33 21.984633279744155 27.35110531283283 28.913228829629716 21.7510325777933 34-35 22.23271057095089 27.3870765250628 28.90101822290299 21.47919468108332 36-37 22.219264760356403 27.314132613475184 28.78093941419049 21.685663211977918 38-39 22.106353864239658 27.48550731539632 28.794378930919716 21.613759889444303 40-41 22.2065344285335 27.313463534837073 28.84398362175866 21.636018414870765 42-43 22.034101822395368 27.347672468993462 28.740723901132842 21.877501807478332 44-45 22.132146513779137 27.50610297048176 28.58811022200219 21.773640293736914 46-47 22.140056524340178 27.4833714332194 28.357878265365365 22.018693777075054 48-49 22.088525999140522 27.540631703108176 28.42246025733008 21.948382040421222 50-51 22.19218823262091 27.66515111856871 28.193732145048795 21.948928503761582 52-53 22.19616952183879 27.727668758662972 28.107094801728127 21.969066917770107 54-55 22.104330004360612 27.72694888526191 28.168465038512952 22.000256071864523 56-57 22.163214116164927 27.737664657495785 28.04506110638015 22.05406011995914 58-59 22.147326809489286 27.642381972762415 28.179409944628915 22.030881273119395 60-61 22.241100769667064 27.82200529082966 28.080254352378493 21.856639587124782 62-63 22.083024127896337 27.726366380125068 28.22212079270363 21.96848869927496 64-65 22.013193097407274 27.549215210900563 28.255773048060156 22.18181864363201 66-67 22.02365970008909 27.685939869104757 28.039335538782485 22.251064892023667 68-69 21.993810601877257 27.739178151153286 28.082293997276803 22.18471724969265 70-71 22.03446071059196 27.528372624011382 28.07976523730387 22.357401428092782 72-73 22.01452756156427 27.68819811407021 27.907538592840336 22.389735731525175 74-75 22.00266741541485 27.729419199849264 27.85229533497861 22.41561804975727 76-77 21.874534222730517 27.699903378095286 27.961933578761517 22.46362882041268 78-79 21.911979822159427 27.609508837965524 27.93733928656063 22.54117205331442 80-81 21.91200223013875 27.784860652985227 27.73280753351861 22.57032958335741 82-83 21.976678114494987 27.785516663166078 27.781504169125643 22.45630105321329 84-85 21.944479357798034 27.69789275314441 27.754611155916315 22.603016733141242 86-87 21.791674196545127 27.701781015930237 27.87779830010468 22.628746487419953 88-89 21.840964193771082 27.584872985915137 27.864415461654435 22.709747358659342 90-91 21.9300293292484 27.626457953259926 27.782350960078627 22.661161757413044 92-93 21.821675630133022 27.59201452417041 27.79203310610974 22.794276739586824 94-95 21.69275269992364 27.515652168540168 27.84825118585521 22.94334394568098 96-97 21.71231986525435 27.449414232070303 27.85662581713828 22.981640085537066 98-99 21.619080203401587 27.377837457475522 27.783989387903844 23.219092951219046 100 20.887265946881314 27.817340966508308 27.927302934193598 23.36809015241678 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1354.0 1 1020.0 2 1064.5 3 1594.0 4 1433.0 5 1385.0 6 2129.5 7 4326.5 8 7033.5 9 8310.0 10 7623.0 11 6518.5 12 6582.5 13 7148.5 14 7860.0 15 8442.5 16 8950.5 17 9706.5 18 10755.5 19 11904.5 20 13392.0 21 15680.0 22 18906.0 23 23588.0 24 29963.0 25 37663.0 26 47522.0 27 59677.0 28 72912.0 29 89041.0 30 108401.0 31 127448.5 32 147587.0 33 169708.5 34 192508.0 35 213186.5 36 236495.5 37 259225.0 38 275857.0 39 290092.0 40 295276.0 41 297482.5 42 303132.0 43 309389.5 44 316184.0 45 324024.5 46 328282.0 47 328009.0 48 328910.0 49 328483.5 50 323798.0 51 311129.0 52 293757.5 53 277726.0 54 260817.0 55 240650.5 56 216383.5 57 190885.5 58 166770.5 59 142630.0 60 118456.0 61 96005.0 62 77265.5 63 60612.0 64 45047.0 65 33353.0 66 24792.0 67 18634.0 68 14259.5 69 11071.5 70 8750.0 71 6736.5 72 5166.5 73 3976.5 74 3123.5 75 2309.0 76 1611.5 77 1139.5 78 789.5 79 514.5 80 338.0 81 235.0 82 167.0 83 109.0 84 76.0 85 63.5 86 49.0 87 33.5 88 24.0 89 14.0 90 10.0 91 8.0 92 5.5 93 6.5 94 5.5 95 4.0 96 3.5 97 4.0 98 3.0 99 3.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0031517629264707567 2 0.06600230274957258 3 8.658689358436144E-4 4 6.926951486748914E-4 5 0.0013969352164943645 6 6.580603912411469E-4 7 0.0 8 8.312341784098698E-4 9 5.772459572290763E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.215574465363453E-4 18-19 0.0010794499400183727 20-21 0.002713055998976658 22-23 5.772459572290762E-6 24-25 1.1544919144581525E-5 26-27 1.0967673187352448E-4 28-29 8.658689358436144E-4 30-31 0.0015239293270847614 32-33 0.002816960271277892 34-35 0.004109991215471023 36-37 0.0012988034037654216 38-39 0.0014027076760666553 40-41 0.005189441155489395 42-43 0.0067075980230018655 44-45 0.005345297563941246 46-47 0.006863454431453716 48-49 0.008370066379821605 50-51 0.008150712916074557 52-53 0.003642421990115471 54-55 0.0021704447991813267 56-57 0.0023436185863500492 58-59 0.002990134058446615 60-61 0.0070019934611886955 62-63 0.0136230045906062 64-65 0.003694374126266088 66-67 0.009778546515460552 68-69 0.012024033289081658 70-71 0.005506926431965388 72-73 0.007238664303652616 74-75 0.002897774705289963 76-77 0.0025629720500970985 78-79 0.007325251197236978 80-81 0.005478064134103934 82-83 0.015816539228076687 84-85 0.017207701984998762 86-87 0.021635178476945776 88-89 0.020498003941204497 90-91 0.02347659308050653 92-93 0.033174325161955016 94-95 0.03688024420736568 96-97 0.02248373003407252 98-99 0.01746746266575185 100 0.017917714512390527 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8661819.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.79279208326144 #Duplication Level Percentage of deduplicated Percentage of total 1 73.95894100847731 45.70129464435036 2 16.171228870939007 19.985307667055373 3 5.059494843185622 9.379209386739076 4 2.024454828725942 5.003868652536671 5 0.9461141727053176 2.9231518180503295 6 0.533063114398597 1.9763674917172989 7 0.3085992298248885 1.3348445631936765 8 0.2036476492209843 1.0067165477245792 9 0.14489426600863958 0.8058079128175777 >10 0.5986180012884301 6.246156671782154 >50 0.03016834728082173 1.28827647369564 >100 0.018775409173194958 2.162186042727238 >500 0.001368608646214115 0.6054214268033326 >1k 5.526938595830038E-4 0.562133832838476 >5k 0.0 0.0 >10k+ 7.895626542834847E-5 1.019256867968115 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25350 0.29266370031514166 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21647 0.24991286472275626 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15676 0.18097815251045998 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.61796765783261E-5 0.0 5 0.0 0.0 0.0 4.61796765783261E-5 0.0 6 0.0 0.0 0.0 4.61796765783261E-5 1.1544919144581525E-5 7 0.0 0.0 0.0 4.61796765783261E-5 1.1544919144581525E-5 8 0.0 0.0 0.0 4.61796765783261E-5 1.1544919144581525E-5 9 0.0 0.0 0.0 4.61796765783261E-5 4.61796765783261E-5 10-11 0.0 0.0 0.0 5.195213615061686E-5 4.61796765783261E-5 12-13 0.0 0.0 0.0 5.772459572290763E-5 4.61796765783261E-5 14-15 0.0 0.0 0.0 5.772459572290763E-5 5.772459572290763E-5 16-17 0.0 0.0 0.0 5.772459572290763E-5 6.926951486748916E-5 18-19 0.0 0.0 0.0 7.504197443977991E-5 6.926951486748916E-5 20-21 0.0 0.0 0.0 8.658689358436144E-5 8.081443401207068E-5 22-23 0.0 0.0 0.0 9.813181272894296E-5 1.2122165101810601E-4 24-25 0.0 0.0 0.0 1.5008394887955982E-4 1.847187063133044E-4 26-27 0.0 0.0 0.0 2.4244330203621202E-4 2.0780854460246745E-4 28-29 0.0 0.0 0.0 8.947312337050682E-4 2.0780854460246745E-4 30-31 0.0 0.0 0.0 0.0035846973943925633 2.0780854460246745E-4 32-33 0.0 0.0 0.0 0.010147983928087162 2.0780854460246745E-4 34-35 0.0 0.0 0.0 0.02098289054527692 2.0780854460246745E-4 36-37 0.0 0.0 0.0 0.03935085690430613 2.0780854460246745E-4 38-39 0.0 0.0 0.0 0.06805729835730809 2.0780854460246745E-4 40-41 0.0 0.0 0.0 0.09965574205602773 2.0780854460246745E-4 42-43 0.0 0.0 0.0 0.1318602940098379 2.0780854460246745E-4 44-45 0.0 0.0 0.0 0.1730929727347108 2.0780854460246745E-4 46-47 0.0 0.0 0.0 0.22462949179612274 2.1935346374704897E-4 48-49 0.0 0.0 0.0 0.2804895830771804 2.4244330203621202E-4 50-51 0.0 0.0 0.0 0.3423703496921374 2.4244330203621202E-4 52-53 0.0 0.0 0.0 0.40373736740516053 2.7707805946995657E-4 54-55 0.0 0.0 0.0 0.47383811645105955 3.174852764759919E-4 56-57 0.0 0.0 0.0 0.5600382552440775 3.2903019562057346E-4 58-59 0.0 0.0 0.0 0.6577082712072372 3.4057511476515503E-4 60-61 0.0 0.0 0.0 0.7644237313201765 3.694374126266088E-4 62-63 0.0 0.0 0.0 0.8726977555176343 3.925272509157719E-4 64-65 0.0 0.0 0.0 0.9874369344360578 3.925272509157719E-4 66-67 0.0 0.0 0.0 1.119141372037444 3.9829971048806266E-4 68-69 0.0 0.0 0.0 1.2723193592477515 4.040721700603534E-4 70-71 0.0 0.0 0.0 1.4433457914555823 4.040721700603534E-4 72-73 0.0 0.0 0.0 1.6207507914907944 4.8488660407242405E-4 74-75 0.0 0.0 0.0 1.8005975419250853 4.8488660407242405E-4 76-77 0.0 0.0 0.0 1.9982523301398933 4.906590636447148E-4 78-79 0.0 0.0 0.0 2.226628148198433 4.964315232170056E-4 80-81 0.0 0.0 0.0 2.4824693289019315 5.195213615061686E-4 82-83 0.0 0.0 0.0 2.7496476202054096 5.426111997953317E-4 84-85 0.0 0.0 0.0 3.0135471544718264 5.426111997953317E-4 86-87 0.0 0.0 0.0 3.2923280895156086 5.657010380844947E-4 88 0.0 0.0 0.0 3.5187874509961476 6.003357955182393E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13115 0.0 45.754414 1 GTATCAA 22760 0.0 38.101257 1 TCAACGC 27820 0.0 30.489985 4 ATCAACG 28235 0.0 30.042013 3 CAACGCA 28280 0.0 29.977072 5 AACGCAG 29550 0.0 28.7679 6 TATCAAC 29835 0.0 28.6396 2 GTACATG 27740 0.0 26.972073 1 ACGCAGA 33255 0.0 25.37882 7 TACATGG 28640 0.0 25.30275 2 CGCAGAG 34085 0.0 24.733253 8 TACCTGG 7475 0.0 24.72404 2 ACATGGG 28310 0.0 24.650528 3 GTACCTG 8145 0.0 23.383772 1 CATGGGG 15720 0.0 22.091696 4 GCAGAGT 38465 0.0 21.391438 9 AGAGTAC 35495 0.0 19.944714 10-11 GAGTACT 22855 0.0 19.420218 12-13 ACCTGGG 9350 0.0 18.84774 3 AGTACTT 24870 0.0 18.555346 12-13 >>END_MODULE