FastQCFastQC Report
Mon 6 Jun 2016
ERR1211472_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211472_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3918633
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131680.33603555117307493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119270.3043663440796829No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74730.19070425834723487No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39900.1018212218393506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69550.044.3445631
TACCTGG46350.041.7931752
GTACCTG51350.037.8709221
GTATCAA115200.037.0724261
ACCTGGG54950.034.6343273
TATAACG4250.034.2949872
TCAACGC142450.029.32494
CAACGCA142350.029.312495
ATCAACG142450.029.1944433
TATCAAC145750.028.6136282
AACGCAG149050.028.1456586
GTACATG132850.027.9647981
TACATGG134050.027.5334572
ATAACGC5000.026.3152473
ACATGGG137050.025.5444553
CATGGGG94000.025.5440834
ACGCAGA167450.024.856537
CGCAGAG169900.024.3890488
ATGGGGG61150.024.2053495
CCTGGGG55300.023.6220114
Produced by FastQC (version 0.11.5)