FastQCFastQC Report
Mon 6 Jun 2016
ERR1211472_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211472_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3918633
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139620.3562977191280735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT131540.33567828372802455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87320.22283280929854876No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40770.10404138381930637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA78700.044.3928761
TACCTGG46700.042.691622
GTACCTG52400.039.035271
ACCTGGG55800.034.8631023
GTATCAA124750.034.4135481
TCAACGC151200.027.9077244
ATCAACG153000.027.3951243
CAACGCA154550.027.2723965
ATAACGC4000.027.0189023
TATCAAC158050.026.5674972
AACGCAG160300.026.3524176
TATAACG4250.025.4467662
TAACGCA4250.025.4295564
GTACATG138300.024.8538931
CCTGGGG53450.024.4397624
CATGGGG90350.023.9237654
TACATGG140900.023.7275162
ATGGGGG56400.023.5780145
ACGCAGA181200.023.1051227
ACATGGG140950.022.8696173
Produced by FastQC (version 0.11.5)