##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211472_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3918633 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12591712467077 33.0 31.0 34.0 30.0 34.0 2 32.3411952586527 34.0 31.0 34.0 30.0 34.0 3 32.34863969144342 34.0 31.0 34.0 30.0 34.0 4 35.98815734976968 37.0 35.0 37.0 35.0 37.0 5 35.914069523734426 37.0 35.0 37.0 35.0 37.0 6 35.930392307725675 37.0 35.0 37.0 35.0 37.0 7 35.85846314263163 37.0 35.0 37.0 35.0 37.0 8 35.85026589629598 37.0 35.0 37.0 35.0 37.0 9 37.50172317744479 39.0 37.0 39.0 35.0 39.0 10-11 37.45908458383319 39.0 37.0 39.0 34.5 39.0 12-13 37.367257535982574 39.0 37.0 39.0 34.0 39.0 14-15 38.60024044099052 40.0 38.0 41.0 34.0 41.0 16-17 38.52120816111129 40.0 38.0 41.0 34.0 41.0 18-19 38.49912865022063 40.0 38.0 41.0 34.0 41.0 20-21 38.43525242093352 40.0 38.0 41.0 34.0 41.0 22-23 38.31943218464194 40.0 38.0 41.0 34.0 41.0 24-25 38.20175109534371 40.0 38.0 41.0 33.5 41.0 26-27 38.01920223200284 40.0 37.0 41.0 33.0 41.0 28-29 38.05530334175208 40.0 37.5 41.0 33.0 41.0 30-31 37.97338753080475 40.0 37.0 41.0 33.0 41.0 32-33 37.778448760065054 40.0 37.0 41.0 33.0 41.0 34-35 37.649312783309895 40.0 37.0 41.0 33.0 41.0 36-37 37.59337631260698 40.0 37.0 41.0 32.5 41.0 38-39 37.49385117207965 40.0 36.5 41.0 32.0 41.0 40-41 37.32045320396169 40.0 36.0 41.0 31.0 41.0 42-43 37.164689446549346 40.0 36.0 41.0 31.0 41.0 44-45 36.974380351515435 39.0 36.0 41.0 30.5 41.0 46-47 36.707375122906384 39.0 35.0 41.0 30.0 41.0 48-49 36.479357980193605 39.0 35.0 41.0 29.5 41.0 50-51 36.269605497631446 39.0 35.0 40.0 29.0 41.0 52-53 36.056980584811086 39.0 35.0 40.0 28.0 41.0 54-55 35.84832861357519 38.0 35.0 40.0 28.0 41.0 56-57 35.63659074478268 38.0 34.0 40.0 27.5 41.0 58-59 35.364497134587495 38.0 34.0 40.0 27.0 41.0 60-61 35.03259401939401 37.5 34.0 40.0 26.0 41.0 62-63 34.685092097167555 37.0 33.0 40.0 26.0 41.0 64-65 34.35470685823347 36.5 33.0 39.5 26.0 41.0 66-67 34.0032261250288 36.0 32.5 39.0 25.0 40.5 68-69 33.60095433790304 35.5 32.0 39.0 24.5 40.0 70-71 33.208593787680556 35.0 32.0 38.0 24.0 40.0 72-73 32.79970808697829 35.0 31.0 37.5 23.0 39.5 74-75 32.392525148438246 35.0 31.0 37.0 22.5 39.0 76-77 30.852935194492566 33.5 29.5 35.0 20.5 37.5 78-79 31.70692037248704 34.0 30.5 36.0 22.0 38.0 80-81 31.699027313861748 34.0 31.0 35.5 22.0 37.0 82-83 31.429436362119137 34.0 30.5 35.0 20.5 37.0 84-85 31.1418780477784 34.0 30.0 35.0 20.0 36.5 86-87 30.85867380282869 34.0 30.0 35.0 20.0 36.0 88-89 30.591626212508288 34.0 29.5 35.0 19.5 36.0 90-91 30.307730910243443 34.0 29.0 35.0 18.5 35.0 92-93 30.101808079501193 34.0 29.0 35.0 18.0 35.0 94-95 29.885699043518493 34.0 29.0 35.0 18.0 35.0 96-97 29.681577733867908 34.0 29.0 35.0 17.5 35.0 98-99 29.518850961547052 33.0 29.0 35.0 17.0 35.0 100 27.63332442716631 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2609226301609979 1101 2 0.24881413523097962 1101 3 0.33049122692357 1101 4 0.1869200194599756 1101 5 0.19726062830230973 1101 6 0.22515154940056448 1101 7 0.22443287796487965 1101 8 0.17297729482647384 1101 9 0.2539478746251689 1101 10-11 0.2540329948501068 1101 12-13 0.1844392584477177 1101 14-15 0.2557126988814389 1101 16-17 0.27044847515922754 1101 18-19 0.3295753935145527 1101 20-21 0.29788843265273357 1101 22-23 0.3103755772128167 1101 24-25 0.2577487387262778 1101 26-27 0.19846881573391073 1101 28-29 0.28610188055801444 1101 30-31 0.26611529078846985 1101 32-33 0.3117483524084008 1101 34-35 0.30405816744899283 1101 36-37 0.29969119978042613 1101 38-39 0.23901567338253216 1101 40-41 0.22097654162563884 1101 42-43 0.2810044357483008 1101 44-45 0.25558957435801943 1101 46-47 0.25925806250009487 1101 48-49 0.264123877868947 1101 50-51 0.1653063625756488 1101 52-53 0.19488104639857085 1101 54-55 0.07432573916091911 1101 56-57 0.05271908354342969 1101 58-59 0.04895983350236577 1101 60-61 0.06845888138151679 1101 62-63 0.17532559058856378 1101 64-65 0.16834849228039417 1101 66-67 -0.032588269884954 1101 68-69 -0.001458437927169598 1101 70-71 -0.04592950153816133 1101 72-73 -0.017996910948802736 1101 74-75 -0.0714083795795446 1101 76-77 0.007450435188133042 1101 78-79 0.03706888637112016 1101 80-81 0.04701890702306244 1101 82-83 0.04613303169296401 1101 84-85 -0.032380723056878224 1101 86-87 -0.09192833895827945 1101 88-89 -0.023204124113206603 1101 90-91 -0.20895514547170535 1101 92-93 -0.17695269510282685 1101 94-95 -0.1317689025127997 1101 96-97 -0.21822953971199155 1101 98-99 -0.35156011173137003 1101 100 -0.2379798967045481 1102 1 0.3459434915056221 1102 2 0.24954624803337566 1102 3 0.31905475683993245 1102 4 0.19921137233281172 1102 5 0.21451637616430475 1102 6 0.16231580275843527 1102 7 0.1656354114553409 1102 8 0.13378951675992568 1102 9 0.24389097470451304 1102 10-11 0.2511301411337712 1102 12-13 0.24320049654334497 1102 14-15 0.37006588696961273 1102 16-17 0.34171216583764163 1102 18-19 0.26764574914334105 1102 20-21 0.2352012004619155 1102 22-23 0.2549755471816866 1102 24-25 0.2981666416865423 1102 26-27 0.22655002518974499 1102 28-29 0.306017367188403 1102 30-31 0.43684430004790187 1102 32-33 0.528353629999124 1102 34-35 0.3864504724260982 1102 36-37 0.45965684280047725 1102 38-39 0.47621554277407796 1102 40-41 0.5000052380077662 1102 42-43 0.3279617021417778 1102 44-45 0.2818092949117741 1102 46-47 0.39261779350351134 1102 48-49 0.32935954259910005 1102 50-51 0.3616340898538297 1102 52-53 0.3418457593355484 1102 54-55 0.3037171728434416 1102 56-57 0.3474033292783858 1102 58-59 0.31831069332093875 1102 60-61 0.3103027974207535 1102 62-63 0.36492832974079903 1102 64-65 0.4016674142658516 1102 66-67 0.4859385833551926 1102 68-69 0.3624849575643907 1102 70-71 0.387261947380388 1102 72-73 0.33743373809340227 1102 74-75 0.2932103488202671 1102 76-77 0.32069994265071955 1102 78-79 0.38613154046128884 1102 80-81 0.3414628302638967 1102 82-83 0.2627450699613405 1102 84-85 0.14411303764599737 1102 86-87 0.19762812874441948 1102 88-89 0.2489463421163478 1102 90-91 0.14864220277496187 1102 92-93 0.07380316580455926 1102 94-95 0.14431791928529236 1102 96-97 0.15189661796219767 1102 98-99 0.1256323503090151 1102 100 0.1837626385642679 1103 1 0.25666357674116824 1103 2 0.2113860466077071 1103 3 0.25204431063346533 1103 4 0.16286987195368852 1103 5 0.11016373809667357 1103 6 0.1365156686892135 1103 7 0.10988418550980583 1103 8 0.15532114828045707 1103 9 0.18985560416116698 1103 10-11 0.2161038256666501 1103 12-13 0.2110090653004093 1103 14-15 0.2920226874757219 1103 16-17 0.31824978868138487 1103 18-19 0.2970181241115526 1103 20-21 0.26356800826713567 1103 22-23 0.2690753439642677 1103 24-25 0.2559972134550108 1103 26-27 0.2951884204326163 1103 28-29 0.29114795737877586 1103 30-31 0.4324789083517899 1103 32-33 0.4082675057787881 1103 34-35 0.4356483988926598 1103 36-37 0.4413016573696851 1103 38-39 0.343199857252138 1103 40-41 0.36595694090539155 1103 42-43 0.4176729585519183 1103 44-45 0.40540907980537355 1103 46-47 0.4477395522264729 1103 48-49 0.41417631186723014 1103 50-51 0.33417526353831306 1103 52-53 0.3242333106412829 1103 54-55 0.40082219525115903 1103 56-57 0.43402410049582585 1103 58-59 0.43809217154574753 1103 60-61 0.3930837897587338 1103 62-63 0.2866781900153086 1103 64-65 0.33447839324253437 1103 66-67 0.4176080525376449 1103 68-69 0.35541694950102 1103 70-71 0.3197549743160053 1103 72-73 0.35590353805357466 1103 74-75 0.32292298867486124 1103 76-77 0.34146061612877077 1103 78-79 0.3656569949984494 1103 80-81 0.36980710906061987 1103 82-83 0.41776129300909304 1103 84-85 0.3015846584208184 1103 86-87 0.415415477481222 1103 88-89 0.3144233746568368 1103 90-91 0.3179717197871277 1103 92-93 0.24555022796660708 1103 94-95 0.2527931977815463 1103 96-97 0.3362487564526724 1103 98-99 0.2814806575470463 1103 100 0.3007687014615108 1104 1 0.17842276024947523 1104 2 0.1410011906997113 1104 3 0.19391547067089476 1104 4 0.042683796328233825 1104 5 0.0572071734015438 1104 6 0.10196927420990676 1104 7 0.1206361784441441 1104 8 0.06656776074657955 1104 9 0.18078103061949946 1104 10-11 0.18401989927664175 1104 12-13 0.215340480521796 1104 14-15 0.27350675915118217 1104 16-17 0.2589041195736854 1104 18-19 0.23194680678581392 1104 20-21 0.1676132950231093 1104 22-23 0.14869080083440878 1104 24-25 0.2161690469233264 1104 26-27 0.3181191389016007 1104 28-29 0.32585836489545983 1104 30-31 0.30051023581935254 1104 32-33 0.2486585556713976 1104 34-35 0.3743518005699755 1104 36-37 0.27444067728328747 1104 38-39 0.2979830609487095 1104 40-41 0.390817940344931 1104 42-43 0.38618194222431157 1104 44-45 0.40616409691336486 1104 46-47 0.38971647037262613 1104 48-49 0.464169279246228 1104 50-51 0.4160708192932674 1104 52-53 0.42355114640421476 1104 54-55 0.45750416752063927 1104 56-57 0.5674019607297467 1104 58-59 0.5465393903322919 1104 60-61 0.4818309950371713 1104 62-63 0.4346457820947549 1104 64-65 0.4509511570107847 1104 66-67 0.5135667732515898 1104 68-69 0.6137541485766818 1104 70-71 0.4912059491770506 1104 72-73 0.5502197923320367 1104 74-75 0.5453708510024313 1104 76-77 0.48450180588207914 1104 78-79 0.5271000467982212 1104 80-81 0.6785976458406857 1104 82-83 0.416136288390728 1104 84-85 0.44115093190581334 1104 86-87 0.5015766673049065 1104 88-89 0.6661798536116592 1104 90-91 0.5445021676639179 1104 92-93 0.4082305861054003 1104 94-95 0.5113711382821506 1104 96-97 0.4050123219827242 1104 98-99 0.3499630648518277 1104 100 0.665456821580829 1105 1 0.25856573754131773 1105 2 0.18364144238958602 1105 3 0.21777367706905437 1105 4 0.12334292475859598 1105 5 0.0641697625676656 1105 6 0.0990190826390247 1105 7 0.12529275259609562 1105 8 0.106815401154023 1105 9 0.17374056711141606 1105 10-11 0.2113318327408038 1105 12-13 0.1893791996446268 1105 14-15 0.2585346381655995 1105 16-17 0.27504856090315144 1105 18-19 0.2614653876038062 1105 20-21 0.23890538610493905 1105 22-23 0.2793016471727583 1105 24-25 0.31996324061238823 1105 26-27 0.36406172993670793 1105 28-29 0.356837401122597 1105 30-31 0.34857583716502205 1105 32-33 0.3860147217617893 1105 34-35 0.39171154888010307 1105 36-37 0.41063978640081444 1105 38-39 0.30542115084684696 1105 40-41 0.3774433336103584 1105 42-43 0.31430408034223944 1105 44-45 0.3661880822106909 1105 46-47 0.3498388888381996 1105 48-49 0.457908026403814 1105 50-51 0.5754797530377758 1105 52-53 0.5071291609199946 1105 54-55 0.4274152350221172 1105 56-57 0.4105750286673171 1105 58-59 0.4423067640979852 1105 60-61 0.5322364088614222 1105 62-63 0.5028545164809444 1105 64-65 0.6411461543630637 1105 66-67 0.5357485008159983 1105 68-69 0.4823016363504138 1105 70-71 0.5601666382092247 1105 72-73 0.48630395892083556 1105 74-75 0.5836308267056367 1105 76-77 0.5434160818064484 1105 78-79 0.7091240009458168 1105 80-81 0.6420856376171535 1105 82-83 0.6112792656825725 1105 84-85 0.6454609652360297 1105 86-87 0.6118408170728422 1105 88-89 0.6271880148426945 1105 90-91 0.623972933635951 1105 92-93 0.5271063921048373 1105 94-95 0.5428604071978249 1105 96-97 0.5390049394951042 1105 98-99 0.6403021297367282 1105 100 0.7372541613933059 1106 1 0.22502978653871253 1106 2 0.19144494939520484 1106 3 0.25084293623508813 1106 4 0.1619933987916582 1106 5 0.1166208910180373 1106 6 0.140797335848184 1106 7 0.14071288012357286 1106 8 0.1733412212731551 1106 9 0.13176378634121733 1106 10-11 0.19362213603805856 1106 12-13 0.2517052651702514 1106 14-15 0.29261861123382005 1106 16-17 0.3070923040863036 1106 18-19 0.2593420446220094 1106 20-21 0.304493050758019 1106 22-23 0.2700818615363829 1106 24-25 0.3311945729389265 1106 26-27 0.28415066357207763 1106 28-29 0.3402299361507133 1106 30-31 0.3534058354870311 1106 32-33 0.38110103353291436 1106 34-35 0.3867501951591592 1106 36-37 0.3944356165879128 1106 38-39 0.3422104683231524 1106 40-41 0.4274754252287849 1106 42-43 0.415301752126787 1106 44-45 0.4674054137821244 1106 46-47 0.5370775938470018 1106 48-49 0.4572373010912898 1106 50-51 0.5531423086213039 1106 52-53 0.6296848493166678 1106 54-55 0.4791328707298135 1106 56-57 0.5609458651468913 1106 58-59 0.5556367938823641 1106 60-61 0.5937782747897771 1106 62-63 0.5999647501414884 1106 64-65 0.5631315872852838 1106 66-67 0.5877280083250085 1106 68-69 0.5507774417871119 1106 70-71 0.6314422276043032 1106 72-73 0.6107941111987074 1106 74-75 0.49968303326659225 1106 76-77 0.49599520966952326 1106 78-79 0.6704867935458587 1106 80-81 0.6838983945598507 1106 82-83 0.6656021954585576 1106 84-85 0.6795380165826224 1106 86-87 0.5998610998146816 1106 88-89 0.6871505221839129 1106 90-91 0.6869160286942595 1106 92-93 0.7435695496683934 1106 94-95 0.9320501987071843 1106 96-97 0.7880169161709993 1106 98-99 0.7869071301562265 1106 100 0.7415494042828108 1107 1 0.14935377842392228 1107 2 0.1244119712525773 1107 3 0.19032024312120654 1107 4 0.12724014512348703 1107 5 0.10329702719020162 1107 6 0.12728475589658217 1107 7 0.14022381891889069 1107 8 0.10199704855746461 1107 9 0.12646225335970485 1107 10-11 0.12222414974019813 1107 12-13 0.12438222444801283 1107 14-15 0.1637500360068458 1107 16-17 0.14307829949645878 1107 18-19 0.1475834504560325 1107 20-21 0.15052986472435492 1107 22-23 0.18599840267312118 1107 24-25 0.2090764956192359 1107 26-27 0.2581735521419475 1107 28-29 0.2546466474689595 1107 30-31 0.13140848593759102 1107 32-33 0.08763840438032133 1107 34-35 0.13874803756097265 1107 36-37 0.2215863906610167 1107 38-39 0.26511835210771295 1107 40-41 0.2338665832043958 1107 42-43 0.2627321377816685 1107 44-45 0.3566607475400687 1107 46-47 0.3359044156315818 1107 48-49 0.3437366633318888 1107 50-51 0.37449048312979016 1107 52-53 0.45419128082956917 1107 54-55 0.3778786667411609 1107 56-57 0.4837373810091563 1107 58-59 0.5867306186527088 1107 60-61 0.47300964412745117 1107 62-63 0.4233493106351105 1107 64-65 0.6235499178552786 1107 66-67 0.5960805923879349 1107 68-69 0.5005341959708787 1107 70-71 0.5505969562911801 1107 72-73 0.5469961612961001 1107 74-75 0.5578343482135821 1107 76-77 0.5563097042473046 1107 78-79 0.7243862036394795 1107 80-81 0.5788793460261097 1107 82-83 0.6372981468237207 1107 84-85 0.5259665850023261 1107 86-87 0.48256581468483617 1107 88-89 0.3433172217074052 1107 90-91 0.2859551350560139 1107 92-93 0.4762974959988746 1107 94-95 0.7351113385188732 1107 96-97 0.6711336050792127 1107 98-99 0.7811833027236439 1107 100 0.9956666172152566 1108 1 0.195846248491776 1108 2 0.14094253210446794 1108 3 0.18304791842703594 1108 4 0.1372517171156673 1108 5 0.1427743517387583 1108 6 0.1952490103467852 1108 7 0.14647256621432803 1108 8 0.13955120393908516 1108 9 0.15071996702184975 1108 10-11 0.16652727768994424 1108 12-13 0.2383054253073027 1108 14-15 0.2767388477491224 1108 16-17 0.3096494608242466 1108 18-19 0.31789977013424675 1108 20-21 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0.05190420902371784 2316 94-95 0.1566741837627852 2316 96-97 0.21000138371903532 2316 98-99 0.004994100298208792 2316 100 -0.015368016726267086 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 3.0 10 13.0 11 12.0 12 44.0 13 145.0 14 438.0 15 1212.0 16 2594.0 17 5346.0 18 9300.0 19 14482.0 20 19589.0 21 26163.0 22 33216.0 23 38653.0 24 42579.0 25 46226.0 26 52716.0 27 62383.0 28 74834.0 29 90170.0 30 107918.0 31 129744.0 32 159071.0 33 200966.0 34 266144.0 35 361447.0 36 529053.0 37 802943.0 38 736373.0 39 104848.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.14384556924165 19.205516200866963 13.689264293884335 23.961373936007046 2 15.66300843372725 20.838699380079547 39.41031583382812 24.087976352365082 3 17.666330407204 26.587983823818757 30.67594787017101 25.06973789880623 4 12.439803348887299 17.107268767153702 37.027955030029744 33.424972853929255 5 12.774892518381034 37.61806900821395 34.69152194789078 14.915516525514235 6 30.91928424492096 36.77634606006273 18.230246850727163 14.07412284428915 7 27.04506903300207 32.68652614317289 22.404751861171995 17.86365296265305 8 26.36993616334426 35.4489810761255 20.18299926376673 17.99808349676351 9 27.050281833047862 15.185375916901615 20.072489583099408 37.69185266695112 10-11 25.218973044936842 25.84673022454514 28.694024166080368 20.24027256443765 12-13 25.490241112142936 23.751165266050688 28.577287028410165 22.181306593396215 14-15 22.899184486018466 25.133535087363374 26.239864769168225 25.72741565744993 16-17 22.594584842105427 27.751921822308745 26.93349417261701 22.719999162968822 18-19 22.38706730345953 26.907616122615373 28.750751225885807 21.954565348039285 20-21 23.20962877915367 26.282562498995155 28.630232004415973 21.877576717435204 22-23 22.97396821799847 26.105647045793773 28.557267291935734 22.36311744427202 24-25 22.660449189978696 26.166596680499303 28.569629473963044 22.60332465555896 26-27 22.592667968568076 26.198981658756797 28.557728439357916 22.650621933317215 28-29 22.64056618194411 26.58718077557821 28.451380199913284 22.320872842564395 30-31 23.147713135514824 26.28955679041488 28.588555788775256 21.974174285295035 32-33 22.891184235022074 26.39954717464566 28.469684319939585 22.239584270392676 34-35 23.07603379540669 26.4546429765866 28.3812026735493 22.088120554457404 36-37 22.93845865280452 26.51736532447299 28.375166035699113 22.169009987023376 38-39 23.043736950771258 26.547294175471208 28.18796733352907 22.221001540228468 40-41 23.012272696590085 26.376784890109224 28.279710653303948 22.331231759996744 42-43 22.816879158481882 26.60758478001055 28.123670517184923 22.451865544322644 44-45 22.71055563750473 26.768277401747337 28.071864716832152 22.449302243915785 46-47 22.68769374413097 26.468142349402918 27.92268520544837 22.92147870101774 48-49 22.750323164343584 26.634930843468506 27.914495755158185 22.70025023702972 50-51 22.74010825414573 26.900020787184786 27.827353816898466 22.532517141771024 52-53 22.860971608072838 26.96046393582398 27.484877399946356 22.693687056156826 54-55 22.67518701475881 26.813500228784132 27.78622196373599 22.725090792721065 56-57 22.815493223367177 26.85396266312521 27.47853217742633 22.85201193608128 58-59 22.82585700063876 26.902701299801635 27.6275921450976 22.643849554462005 60-61 23.049035372134185 26.865181788797738 27.68086206234323 22.40492077672485 62-63 22.958228137573084 26.968726104440634 27.575985964597056 22.497059793389234 64-65 22.809639416371716 26.71690843299639 27.620415505813135 22.853036644818758 66-67 22.664841685523047 26.754536633272203 27.641535148412004 22.939086532792746 68-69 22.520417613069725 26.813058135340007 27.637189680930252 23.02933457066002 70-71 22.509425066178032 26.63658641935531 27.688544389525592 23.165444124941065 72-73 22.455962508316727 27.064710822959597 27.336192730078206 23.143133938645473 74-75 22.625703741643207 27.01145509311043 27.202739096071753 23.160102069174606 76-77 22.455914746285934 26.813419520734318 27.429660543642104 23.301005189337644 78-79 22.399513566418218 26.840848681129515 27.531668587135034 23.227969165317234 80-81 22.493892647123563 26.939343729922616 27.203454442560897 23.363309180392925 82-83 22.57343360570904 26.984924687244366 27.26192861399772 23.179713093048868 84-85 22.52582597958016 27.134825983153455 27.063423919729534 23.275924117536857 86-87 22.329383510340893 27.126971832576718 27.135012119775777 23.408632537306616 88-89 22.10962746438582 26.888295214657564 27.346589713349616 23.655487607607 90-91 22.524828987627686 26.82014366085807 27.227066193701965 23.427961157812277 92-93 22.2829542922071 26.930725446653646 27.204613283087802 23.581706978051447 94-95 22.13203330586437 26.809939272143918 27.1741583417323 23.883869080259412 96-97 22.037680763227975 26.773498980340264 27.24026179966274 23.948558456769018 98-99 22.00193624777846 26.74473510849088 27.08660305925107 24.166725584479593 100 21.196592540599177 27.240532176243498 27.28849184825958 24.274383434897747 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1475.0 1 1106.0 2 1770.5 3 2646.5 4 1824.5 5 1322.5 6 1872.5 7 3441.5 8 5408.5 9 6356.0 10 5525.5 11 4377.5 12 4315.0 13 4608.5 14 4970.0 15 5222.0 16 5396.0 17 5591.5 18 5982.0 19 6407.5 20 6883.5 21 7536.0 22 8661.0 23 10486.0 24 12823.5 25 15771.0 26 19474.5 27 23904.0 28 29100.0 29 34892.5 30 41135.0 31 47445.5 32 53900.5 33 60159.5 34 66687.0 35 72879.0 36 80951.5 37 89225.5 38 95631.5 39 101809.0 40 106823.0 41 112630.0 42 118540.5 43 124531.0 44 130373.0 45 135739.5 46 141698.5 47 146848.5 48 152388.0 49 157389.5 50 159923.0 51 158796.0 52 153324.0 53 147689.0 54 141254.5 55 131415.5 56 120750.0 57 110649.5 58 99390.0 59 85938.5 60 70409.5 61 56537.5 62 45912.5 63 36048.5 64 27123.5 65 20450.5 66 15548.5 67 11973.5 68 9253.0 69 7006.5 70 5549.0 71 4419.5 72 3483.0 73 2754.5 74 2180.0 75 1650.0 76 1183.0 77 849.0 78 586.5 79 392.5 80 273.0 81 195.5 82 131.0 83 102.5 84 81.5 85 61.0 86 43.5 87 29.0 88 23.5 89 13.5 90 5.5 91 9.0 92 8.5 93 3.0 94 1.0 95 1.5 96 3.0 97 3.0 98 1.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003547155347285648 2 0.07165764183581366 3 8.166113029722354E-4 4 4.5934385792188243E-4 5 0.001429069780201412 6 7.655730965364707E-4 7 0.0 8 9.697259222795297E-4 9 2.5519103217882355E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.486607191844707E-4 18-19 9.697259222795297E-4 20-21 0.0033174834183247064 22-23 0.0 24-25 3.827865482682354E-5 26-27 1.4035506769835297E-4 28-29 8.676495094080002E-4 30-31 0.0014801079866371767 32-33 0.0031771283506263535 34-35 0.004376526201866824 36-37 0.0012249169544583532 38-39 0.001492867538246118 40-41 0.005601443156325178 42-43 0.007298463520314355 44-45 0.005486607191844707 46-47 0.00736226127835906 48-49 0.00888064791982306 50-51 0.009021002987521414 52-53 0.004083056514861178 54-55 0.002347757496045177 56-57 0.0023987957024809415 58-59 0.002858139560402824 60-61 0.007872643342716708 62-63 0.013805834840874357 64-65 0.004593438579218825 66-67 0.010692504248292708 68-69 0.01297646398629318 70-71 0.005754557775632472 72-73 0.008268189442593886 74-75 0.002909177766838589 76-77 0.002998494628101177 78-79 0.00811507482328659 80-81 0.005116580195185413 82-83 0.016281187853008944 84-85 0.017327471084942123 86-87 0.022010226525423535 88-89 0.021601920873937416 90-91 0.023834842405502123 92-93 0.03409352189909083 94-95 0.0378958682785553 96-97 0.02308202886057459 98-99 0.01903725100054024 100 0.019343480239154828 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3918633.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.92045303679247 #Duplication Level Percentage of deduplicated Percentage of total 1 77.0314122815292 50.77945595662345 2 14.82274159758276 19.542436827199296 3 4.120336930861298 8.148434314398116 4 1.576110136416519 4.155915769138309 5 0.7723230623768451 2.545594308132228 6 0.458447955611117 1.8132658156605697 7 0.2765658011711381 1.2761940035379427 8 0.19261218601787344 1.0157666050164131 9 0.14187623163931481 0.8417290918334888 >10 0.5799719188682871 6.304358303019125 >50 0.01862144510150651 0.8345939503909944 >100 0.007841128365679595 0.9291654864057839 >500 6.835946788646948E-4 0.28720069663000425 >1k 3.1901084568158216E-4 0.44894635579189707 >5k 4.557297795451174E-5 0.2623259889437129 >10k+ 9.114595590902348E-5 0.8146165272787127 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13962 0.3562977191280735 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13154 0.33567828372802455 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8732 0.22283280929854876 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4077 0.10404138381930637 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 2.551910321788236E-5 7 0.0 0.0 0.0 0.0 2.551910321788236E-5 8 0.0 0.0 0.0 0.0 2.551910321788236E-5 9 0.0 0.0 0.0 0.0 5.103820643576472E-5 10-11 0.0 0.0 0.0 0.0 5.103820643576472E-5 12-13 0.0 0.0 0.0 0.0 5.103820643576472E-5 14-15 0.0 0.0 0.0 0.0 1.2759551608941178E-4 16-17 0.0 0.0 0.0 2.551910321788236E-5 1.5311461930729415E-4 18-19 0.0 0.0 0.0 2.551910321788236E-5 1.5311461930729415E-4 20-21 0.0 0.0 0.0 2.551910321788236E-5 1.5311461930729415E-4 22-23 0.0 0.0 0.0 8.931686126258825E-5 1.5311461930729415E-4 24-25 0.0 0.0 0.0 2.6795058378776475E-4 1.5311461930729415E-4 26-27 0.0 0.0 0.0 4.5934385792188243E-4 1.5311461930729415E-4 28-29 0.0 0.0 0.0 0.001212157402849412 1.786337225251765E-4 30-31 0.0 0.0 0.0 0.003751308173028707 2.0415282574305887E-4 32-33 0.0 0.0 0.0 0.010896657074035768 2.2967192896094122E-4 34-35 0.0 0.0 0.0 0.02240577262530071 2.2967192896094122E-4 36-37 0.0 1.275955160894118E-5 0.0 0.04201720344824331 2.2967192896094122E-4 38-39 0.0 2.551910321788236E-5 0.0 0.07461785780908801 2.5519103217882356E-4 40-41 0.0 2.551910321788236E-5 0.0 0.1125392451908612 2.5519103217882356E-4 42-43 0.0 2.551910321788236E-5 0.0 0.15298702379120474 2.6795058378776475E-4 44-45 1.275955160894118E-5 2.551910321788236E-5 0.0 0.20052911308611956 3.189887902235295E-4 46-47 2.551910321788236E-5 2.551910321788236E-5 0.0 0.2578194998102655 3.317483418324707E-4 48-49 5.103820643576472E-5 2.551910321788236E-5 0.0 0.31847840815917183 3.317483418324707E-4 50-51 5.103820643576472E-5 5.103820643576472E-5 0.0 0.3886814611115662 3.317483418324707E-4 52-53 5.103820643576472E-5 7.655730965364708E-5 0.0 0.4609898400794359 3.4450789344141186E-4 54-55 5.103820643576472E-5 7.655730965364708E-5 0.0 0.5467595459947385 3.57267445050353E-4 56-57 5.103820643576472E-5 7.655730965364708E-5 0.0 0.6439362910484345 3.8278654826823537E-4 58-59 5.103820643576472E-5 7.655730965364708E-5 0.0 0.7589764083546482 4.0830565148611774E-4 60-61 5.103820643576472E-5 7.655730965364708E-5 0.0 0.879401056439835 4.0830565148611774E-4 62-63 5.103820643576472E-5 7.655730965364708E-5 0.0 1.0049040060653804 4.338247547040001E-4 64-65 5.103820643576472E-5 7.655730965364708E-5 0.0 1.1360977157085137 4.338247547040001E-4 66-67 5.103820643576472E-5 1.0207641287152944E-4 0.0 1.2885360787805338 4.338247547040001E-4 68-69 5.103820643576472E-5 1.0207641287152944E-4 0.0 1.4692496082179678 4.593438579218825E-4 70-71 5.103820643576472E-5 1.0207641287152944E-4 0.0 1.667277849188735 4.97622512748706E-4 72-73 5.103820643576472E-5 1.0207641287152944E-4 0.0 1.8621417213604845 6.890157868828237E-4 74-75 5.103820643576472E-5 1.0207641287152944E-4 0.0 2.061445917492146 7.14534890100706E-4 76-77 5.103820643576472E-5 1.5311461930729415E-4 0.0 2.2907605790080368 7.14534890100706E-4 78-79 5.103820643576472E-5 1.5311461930729415E-4 0.0 2.5571672570511197 7.272944417096472E-4 80-81 5.103820643576472E-5 1.5311461930729415E-4 0.0 2.8324801021172434 7.528135449275295E-4 82-83 5.103820643576472E-5 1.5311461930729415E-4 0.0 3.1220964045369906 7.655730965364707E-4 84-85 5.103820643576472E-5 1.5311461930729415E-4 0.0 3.4189728918222246 7.655730965364707E-4 86-87 5.103820643576472E-5 1.5311461930729415E-4 0.0 3.737566135945877 7.78332648145412E-4 88 5.103820643576472E-5 1.5311461930729415E-4 0.0 3.992693370366656 7.910921997543531E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7870 0.0 44.392876 1 TACCTGG 4670 0.0 42.69162 2 GTACCTG 5240 0.0 39.03527 1 ACCTGGG 5580 0.0 34.863102 3 GTATCAA 12475 0.0 34.413548 1 TCAACGC 15120 0.0 27.907724 4 ATCAACG 15300 0.0 27.395124 3 CAACGCA 15455 0.0 27.272396 5 ATAACGC 400 0.0 27.018902 3 TATCAAC 15805 0.0 26.567497 2 AACGCAG 16030 0.0 26.352417 6 TATAACG 425 0.0 25.446766 2 TAACGCA 425 0.0 25.429556 4 GTACATG 13830 0.0 24.853893 1 CCTGGGG 5345 0.0 24.439762 4 CATGGGG 9035 0.0 23.923765 4 TACATGG 14090 0.0 23.727516 2 ATGGGGG 5640 0.0 23.578014 5 ACGCAGA 18120 0.0 23.105122 7 ACATGGG 14095 0.0 22.869617 3 >>END_MODULE