FastQCFastQC Report
Mon 6 Jun 2016
ERR1211471_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211471_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6758679
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260880.38599258819659876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224880.3327277416193313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145280.21495324752070633No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA132800.051.4795341
GTATCAA225400.038.0383871
TACCTGG50950.035.8085822
GTACATG336450.031.234871
TCAACGC281900.029.856334
ATCAACG281900.029.7740823
GTACCTG63250.029.7015041
TACATGG351750.029.6768972
ATGGGGG131450.029.5978855
CAACGCA285700.029.4397225
ACCTGGG60300.029.1478353
ACATGGG351950.028.7484913
TATCAAC294050.028.720022
AACGCAG296750.028.4150876
CATGGGG213100.027.7196524
TGGGGGG129600.025.735266
CGCAGAG336850.024.6439658
ACGCAGA347350.024.208837
GCAGAGT364250.022.2628489
GAGTACT185900.021.86252612-13
Produced by FastQC (version 0.11.5)