FastQCFastQC Report
Mon 6 Jun 2016
ERR1211471_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211471_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6758679
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT287670.42563051152451536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT255250.37766255802354276No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186800.2763853705731549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA154600.048.5538671
TACCTGG49850.036.7005042
GTATCAA250650.034.957921
ACCTGGG57750.030.7588843
GTACCTG63750.029.5845281
GTACATG335550.028.6639841
TACATGG350900.027.0741962
TCAACGC319150.026.9304264
ATCAACG319700.026.8550953
CAACGCA322000.026.7502465
ATGGGGG120550.026.7020455
ACATGGG349350.026.1765773
TATCAAC330700.026.0542682
AACGCAG334500.025.7921816
CATGGGG199200.024.7699034
TGGGGGG113250.024.273326
CGCAGAG380750.022.2765948
TATAACG4250.022.1324062
ACGCAGA390850.021.9534197
GAGTACT218650.021.05085212-13
Produced by FastQC (version 0.11.5)