##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211471_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6758679 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09650717248149 33.0 31.0 34.0 30.0 34.0 2 32.315261606595016 34.0 31.0 34.0 30.0 34.0 3 32.31802279705842 34.0 31.0 34.0 30.0 34.0 4 35.96474917657726 37.0 35.0 37.0 35.0 37.0 5 35.8915138594391 37.0 35.0 37.0 35.0 37.0 6 35.91248215812587 37.0 35.0 37.0 35.0 37.0 7 35.8416811924342 37.0 35.0 37.0 35.0 37.0 8 35.83189688399168 37.0 35.0 37.0 35.0 37.0 9 37.47211829412227 39.0 37.0 39.0 34.0 39.0 10-11 37.435235421004606 39.0 37.0 39.0 34.0 39.0 12-13 37.34836563476384 39.0 37.0 39.0 34.0 39.0 14-15 38.554315717612866 40.0 38.0 41.0 34.0 41.0 16-17 38.47933272167535 40.0 38.0 41.0 34.0 41.0 18-19 38.4810812142432 40.0 38.0 41.0 34.0 41.0 20-21 38.432582757666104 40.0 38.0 41.0 34.0 41.0 22-23 38.34000556913563 40.0 38.0 41.0 34.0 41.0 24-25 38.2332807934805 40.0 38.0 41.0 33.5 41.0 26-27 38.05620306867659 40.0 37.0 41.0 33.0 41.0 28-29 38.09241243740085 40.0 37.5 41.0 33.0 41.0 30-31 38.00373423563984 40.0 37.0 41.0 33.0 41.0 32-33 37.815623807551745 40.0 37.0 41.0 33.0 41.0 34-35 37.712301102034886 40.0 37.0 41.0 33.0 41.0 36-37 37.67625855585093 40.0 37.0 41.0 33.0 41.0 38-39 37.59869635767581 40.0 37.0 41.0 32.5 41.0 40-41 37.44496905386393 40.0 36.0 41.0 32.0 41.0 42-43 37.30698839225831 40.0 36.0 41.0 31.0 41.0 44-45 37.130579215257896 40.0 36.0 41.0 31.0 41.0 46-47 36.87758525001705 39.0 36.0 41.0 30.0 41.0 48-49 36.667291566887556 39.0 35.0 41.0 30.0 41.0 50-51 36.48440309119578 39.0 35.0 41.0 29.5 41.0 52-53 36.307205150592296 39.0 35.0 40.0 29.0 41.0 54-55 36.12667837901459 39.0 35.0 40.0 28.0 41.0 56-57 35.951499324054296 38.5 35.0 40.0 28.0 41.0 58-59 35.72039358578799 38.0 34.0 40.0 28.0 41.0 60-61 35.42173433595529 38.0 34.0 40.0 27.0 41.0 62-63 35.10607760776921 38.0 34.0 40.0 26.0 41.0 64-65 34.80994984374905 37.0 33.5 40.0 26.0 41.0 66-67 34.47149701887011 37.0 33.0 39.5 26.0 41.0 68-69 34.08209289122919 36.0 33.0 39.0 25.5 40.0 70-71 33.69036708208809 36.0 32.0 39.0 25.0 40.0 72-73 33.26196213786747 35.0 32.0 38.0 24.5 40.0 74-75 32.83722551403906 35.0 31.0 37.0 23.5 39.0 76-77 31.256780947874578 33.5 29.5 35.5 21.5 38.5 78-79 32.09636076813236 34.5 31.0 36.0 23.0 39.0 80-81 32.05513118761817 35.0 31.0 36.0 23.0 37.5 82-83 31.754196863026046 34.0 31.0 35.5 22.5 37.0 84-85 31.443342774527387 34.0 31.0 35.0 21.5 37.0 86-87 31.145574675169513 34.0 30.0 35.0 20.0 36.0 88-89 30.864060269765734 34.0 30.0 35.0 20.0 36.0 90-91 30.565468488738702 34.0 29.5 35.0 19.5 35.5 92-93 30.347294049621237 34.0 29.0 35.0 19.0 35.0 94-95 30.12149741095856 34.0 29.0 35.0 18.0 35.0 96-97 29.919737643998182 34.0 29.0 35.0 18.0 35.0 98-99 29.760454150877706 34.0 29.0 35.0 18.0 35.0 100 27.897168958608628 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24799412103298835 1101 2 0.14970350386791864 1101 3 0.2387014353463499 1101 4 0.1737278517550962 1101 5 0.17818143691783916 1101 6 0.17892553605008743 1101 7 0.19467422618002672 1101 8 0.20194628724393482 1101 9 0.22555762214928876 1101 10-11 0.18673896896436304 1101 12-13 0.23138959032419848 1101 14-15 0.3233703390888323 1101 16-17 0.30214075342554736 1101 18-19 0.29956373461718044 1101 20-21 0.30055647240513395 1101 22-23 0.2615293473156086 1101 24-25 0.20914691518447626 1101 26-27 0.17003031298221316 1101 28-29 0.2765049207466106 1101 30-31 0.33410765552776667 1101 32-33 0.3950019842860115 1101 34-35 0.33797600699165287 1101 36-37 0.3256822316176837 1101 38-39 0.32938402305006775 1101 40-41 0.3203448398844415 1101 42-43 0.2992981081689621 1101 44-45 0.2693468059750259 1101 46-47 0.38503108594149893 1101 48-49 0.41354730895433534 1101 50-51 0.4231300670228535 1101 52-53 0.34399849290664264 1101 54-55 0.29681821743539416 1101 56-57 0.29696582548199757 1101 58-59 0.26955331884158795 1101 60-61 0.24081844139537623 1101 62-63 0.24563551465112 1101 64-65 0.3364534189878441 1101 66-67 0.20592343041127492 1101 68-69 0.21378594185348732 1101 70-71 0.1980754547586443 1101 72-73 0.06298172518237521 1101 74-75 0.12670953766912163 1101 76-77 0.1808752019892843 1101 78-79 0.26890820197170484 1101 80-81 0.23039525917325676 1101 82-83 0.26602670543251605 1101 84-85 0.21955143844190417 1101 86-87 0.21957090648058042 1101 88-89 0.21518286983335955 1101 90-91 0.10010441092159184 1101 92-93 0.14765672005129815 1101 94-95 0.1731126778731955 1101 96-97 0.07400611695128134 1101 98-99 -0.138033495608628 1101 100 -0.12123071092941373 1102 1 0.28532414886632296 1102 2 0.20299983098654195 1102 3 0.2747861312077191 1102 4 0.1848826554531513 1102 5 0.18031191092374144 1102 6 0.15558752886097693 1102 7 0.1741664076701568 1102 8 0.1929815754378481 1102 9 0.2069931718926341 1102 10-11 0.20051267625277092 1102 12-13 0.23663370895854996 1102 14-15 0.2883437295807454 1102 16-17 0.26953531193849045 1102 18-19 0.25956962647278203 1102 20-21 0.2745624621669407 1102 22-23 0.25623071590450053 1102 24-25 0.24051588870784002 1102 26-27 0.19305255517419795 1102 28-29 0.29075060588025536 1102 30-31 0.2938534140910676 1102 32-33 0.3719672765360329 1102 34-35 0.35611124343515144 1102 36-37 0.3767252124420466 1102 38-39 0.31135178043849265 1102 40-41 0.4028783902431954 1102 42-43 0.32199052882997137 1102 44-45 0.3513893916779196 1102 46-47 0.3989428060136717 1102 48-49 0.3823610179211485 1102 50-51 0.36479690269428744 1102 52-53 0.3116063645655984 1102 54-55 0.314617810425311 1102 56-57 0.27882915541191977 1102 58-59 0.37443653072460137 1102 60-61 0.38609672230226977 1102 62-63 0.49090699457335063 1102 64-65 0.42786139764429976 1102 66-67 0.4136108683457351 1102 68-69 0.2953895056393776 1102 70-71 0.34304582064812905 1102 72-73 0.3559399611922842 1102 74-75 0.3165105782722222 1102 76-77 0.3110076725824591 1102 78-79 0.26549889808482874 1102 80-81 0.3413251408466209 1102 82-83 0.35936803443932774 1102 84-85 0.27177205609019595 1102 86-87 0.2116078975431428 1102 88-89 0.28457714511763754 1102 90-91 0.25201643170568033 1102 92-93 0.18450050370655546 1102 94-95 0.2761176116466437 1102 96-97 0.2076972858115127 1102 98-99 0.12642922660349143 1102 100 0.11503462188175462 1103 1 0.2907018391732237 1103 2 0.2623523082220416 1103 3 0.3147243929052692 1103 4 0.15875361748956607 1103 5 0.15732233785004013 1103 6 0.2112663096011289 1103 7 0.22874767299839505 1103 8 0.2472187092229774 1103 9 0.3006831215413328 1103 10-11 0.2116136866992946 1103 12-13 0.26435619619531536 1103 14-15 0.3748559218576375 1103 16-17 0.38879403059537054 1103 18-19 0.3759757113411766 1103 20-21 0.3076757040651401 1103 22-23 0.3301583352143993 1103 24-25 0.33231870604777214 1103 26-27 0.3730824668818187 1103 28-29 0.3933783646699496 1103 30-31 0.4198888783868924 1103 32-33 0.5168543668926944 1103 34-35 0.49879153591297154 1103 36-37 0.5906638482003856 1103 38-39 0.5383620063747188 1103 40-41 0.5641388442848836 1103 42-43 0.5597879466264928 1103 44-45 0.5780418004744732 1103 46-47 0.5905982939780898 1103 48-49 0.5741573633808272 1103 50-51 0.5877896843003683 1103 52-53 0.5476863222158883 1103 54-55 0.553067276551829 1103 56-57 0.5855795421168111 1103 58-59 0.6613860152691231 1103 60-61 0.6475962046980257 1103 62-63 0.7002067612494614 1103 64-65 0.6714427797660321 1103 66-67 0.6802980323451564 1103 68-69 0.6736647850009589 1103 70-71 0.6994940900432312 1103 72-73 0.6225356016226513 1103 74-75 0.5934241170217902 1103 76-77 0.6498075886490042 1103 78-79 0.6556712730156491 1103 80-81 0.5699882908448473 1103 82-83 0.5669972630145992 1103 84-85 0.4935904344467694 1103 86-87 0.49109940801494645 1103 88-89 0.5999606765613983 1103 90-91 0.6429456621519769 1103 92-93 0.5964196752941895 1103 94-95 0.6823698864887966 1103 96-97 0.6607557261227228 1103 98-99 0.5981549850815462 1103 100 0.5788250183277235 1104 1 0.25163842746852794 1104 2 0.1677918539644594 1104 3 0.26636216693297143 1104 4 0.09514080507602074 1104 5 0.1338605906026089 1104 6 0.18054255784574025 1104 7 0.21927495874246006 1104 8 0.19819530271069397 1104 9 0.22144668180677485 1104 10-11 0.2698716579076148 1104 12-13 0.3143746515569461 1104 14-15 0.3512655115651171 1104 16-17 0.32211387479672027 1104 18-19 0.3299234377586302 1104 20-21 0.24283829809569824 1104 22-23 0.27612559284600735 1104 24-25 0.31154978618796747 1104 26-27 0.35388282641716984 1104 28-29 0.4092937374944796 1104 30-31 0.3594359320195508 1104 32-33 0.38878104108574263 1104 34-35 0.5199794711546488 1104 36-37 0.32015553247013173 1104 38-39 0.38344555376401956 1104 40-41 0.5012054874807816 1104 42-43 0.6426728441746477 1104 44-45 0.5475445612392278 1104 46-47 0.552609177724392 1104 48-49 0.5646536716528985 1104 50-51 0.5436246599314316 1104 52-53 0.5453529318446684 1104 54-55 0.5116018815021945 1104 56-57 0.5887710412428859 1104 58-59 0.6260278534100365 1104 60-61 0.569696221612709 1104 62-63 0.5519297315202678 1104 64-65 0.6355634096478511 1104 66-67 0.6486216003992169 1104 68-69 0.6545889724733414 1104 70-71 0.516585803684201 1104 72-73 0.6557641032570913 1104 74-75 0.6377644345458009 1104 76-77 0.6046305515770065 1104 78-79 0.5900724604526388 1104 80-81 0.6307593709325943 1104 82-83 0.5847026911855409 1104 84-85 0.5402098907904964 1104 86-87 0.6208770684389151 1104 88-89 0.6460731841172809 1104 90-91 0.47362788114860876 1104 92-93 0.44170588746725414 1104 94-95 0.4691948890128863 1104 96-97 0.4687593214762984 1104 98-99 0.5023273227563116 1104 100 0.666237052141355 1105 1 0.2087699241772114 1105 2 0.15358081077154395 1105 3 0.20278380034977062 1105 4 0.10719675127662498 1105 5 0.10386667273465378 1105 6 0.1360666843754501 1105 7 0.16285433554434547 1105 8 0.14996405894591902 1105 9 0.12132146357102158 1105 10-11 0.18166121776818756 1105 12-13 0.21854697241579402 1105 14-15 0.3019742351927306 1105 16-17 0.27445192909198823 1105 18-19 0.30995932586804287 1105 20-21 0.22379211833814594 1105 22-23 0.30048098778041066 1105 24-25 0.2992443177818842 1105 26-27 0.30812241824537523 1105 28-29 0.3690382426878287 1105 30-31 0.397817498316563 1105 32-33 0.4517774456667354 1105 34-35 0.4756280917490088 1105 36-37 0.45837532799498604 1105 38-39 0.42569383166251384 1105 40-41 0.4554430968905976 1105 42-43 0.3928797896453844 1105 44-45 0.44152811151159455 1105 46-47 0.525225036727555 1105 48-49 0.5157183615859182 1105 50-51 0.46448088725525594 1105 52-53 0.4606355400700082 1105 54-55 0.5260270349336835 1105 56-57 0.5222341098292276 1105 58-59 0.5435895457725479 1105 60-61 0.5958688515115753 1105 62-63 0.5968829514248668 1105 64-65 0.6485202337520306 1105 66-67 0.5826638610332893 1105 68-69 0.677757233719511 1105 70-71 0.6803746687025978 1105 72-73 0.6267308707736348 1105 74-75 0.643144944367684 1105 76-77 0.6343056531171065 1105 78-79 0.7836074500920063 1105 80-81 0.735997617341404 1105 82-83 0.7000492618234944 1105 84-85 0.6788012675601571 1105 86-87 0.676974358291929 1105 88-89 0.6812577160397844 1105 90-91 0.7103547526850953 1105 92-93 0.6828446899761076 1105 94-95 0.791127886348935 1105 96-97 0.6967556043060306 1105 98-99 0.7650825330995055 1105 100 0.8932856937185711 1106 1 0.22559996291538909 1106 2 0.20647129155869948 1106 3 0.24825925028391538 1106 4 0.15672445848843353 1106 5 0.18992564428094028 1106 6 0.16526237168513802 1106 7 0.19631634797816844 1106 8 0.18071213027659638 1106 9 0.14884055373039473 1106 10-11 0.20280972612292913 1106 12-13 0.25230380430129173 1106 14-15 0.29718084455615923 1106 16-17 0.32991367954291917 1106 18-19 0.35979072223032205 1106 20-21 0.3647008497910278 1106 22-23 0.39257070095582236 1106 24-25 0.37749293133124695 1106 26-27 0.34354826602285726 1106 28-29 0.36846166558301974 1106 30-31 0.33486537125541105 1106 32-33 0.4461677906541368 1106 34-35 0.4058140575887137 1106 36-37 0.40943833338692315 1106 38-39 0.3788266232844393 1106 40-41 0.43353856341948216 1106 42-43 0.4795275131251415 1106 44-45 0.5265054933128184 1106 46-47 0.46343602594825484 1106 48-49 0.5043070645959986 1106 50-51 0.47784254698317596 1106 52-53 0.5331516859561987 1106 54-55 0.43247068438339653 1106 56-57 0.5126983433289922 1106 58-59 0.5980150259710371 1106 60-61 0.6939302808047145 1106 62-63 0.6046458238553569 1106 64-65 0.6165330654986079 1106 66-67 0.6414975628636981 1106 68-69 0.6091192656013149 1106 70-71 0.6455204309985945 1106 72-73 0.5986976181794574 1106 74-75 0.5933770550488262 1106 76-77 0.614463464665512 1106 78-79 0.7478632268101322 1106 80-81 0.7406221569223419 1106 82-83 0.6621932846315062 1106 84-85 0.7524731165272946 1106 86-87 0.6496750499323696 1106 88-89 0.7161323913971351 1106 90-91 0.6543409708537276 1106 92-93 0.7361745382958595 1106 94-95 0.8237890396730663 1106 96-97 0.8216583107125572 1106 98-99 0.7503967860612626 1106 100 0.776684353161631 1107 1 0.19755998982419243 1107 2 0.20974695224103357 1107 3 0.23147023359783958 1107 4 0.11654820639252961 1107 5 0.12314909299708887 1107 6 0.12153763814054486 1107 7 0.15004587281167403 1107 8 0.144560945121043 1107 9 0.1909372005326233 1107 10-11 0.1425729045232771 1107 12-13 0.1544269665007434 1107 14-15 0.2038750628876116 1107 16-17 0.2083282688483976 1107 18-19 0.11972373567474648 1107 20-21 0.20199983088505746 1107 22-23 0.2402960921247157 1107 24-25 0.2781394945901212 1107 26-27 0.2543803940623448 1107 28-29 0.24806998754959153 1107 30-31 0.16877355252075432 1107 32-33 0.17010201248779566 1107 34-35 0.2371533962115251 1107 36-37 0.3653822351429028 1107 38-39 0.29362808304937005 1107 40-41 0.2691634936017877 1107 42-43 0.21460779900690596 1107 44-45 0.3056252721505146 1107 46-47 0.304336396689898 1107 48-49 0.31879235601606126 1107 50-51 0.3223533282066242 1107 52-53 0.47098618987848084 1107 54-55 0.4048413605241947 1107 56-57 0.4239375708198878 1107 58-59 0.48367307066588694 1107 60-61 0.4405028898742458 1107 62-63 0.40985009497331504 1107 64-65 0.5195917046719174 1107 66-67 0.605575887491689 1107 68-69 0.4831873589931206 1107 70-71 0.5532217074466317 1107 72-73 0.5209323368085634 1107 74-75 0.5181257959974559 1107 76-77 0.47554925106038937 1107 78-79 0.5572730272969082 1107 80-81 0.6150875512732341 1107 82-83 0.5538884814398557 1107 84-85 0.4498381271999712 1107 86-87 0.3860894403403492 1107 88-89 0.40241477662646474 1107 90-91 0.3505526870875606 1107 92-93 0.4185038311310727 1107 94-95 0.5711422985592058 1107 96-97 0.6280774850903157 1107 98-99 0.686668755246238 1107 100 0.8760103614434129 1108 1 0.2353939408071426 1108 2 0.17540631426469844 1108 3 0.2154253377991111 1108 4 0.16432104822942506 1108 5 0.13209198576186054 1108 6 0.07160266426388517 1108 7 0.1491223549288634 1108 8 0.12386203166116161 1108 9 0.14529214103075816 1108 10-11 0.16520511325767728 1108 12-13 0.19790394503120012 1108 14-15 0.23988937544376654 1108 16-17 0.2901958983102375 1108 18-19 0.33019786911071947 1108 20-21 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74-75 -0.48089302596256545 2316 76-77 -0.4664457018928907 2316 78-79 -0.31358913523033394 2316 80-81 -0.2769690758618175 2316 82-83 -0.1289681526128028 2316 84-85 -0.23435855898711822 2316 86-87 -0.2841622903460994 2316 88-89 -0.24364632545073306 2316 90-91 -0.15525272537693624 2316 92-93 -0.014181896028329533 2316 94-95 -5.410022972220929E-4 2316 96-97 0.09105876689434922 2316 98-99 -0.09202020916502462 2316 100 -0.342309511384844 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 20.0 11 29.0 12 85.0 13 210.0 14 617.0 15 1620.0 16 3702.0 17 7617.0 18 13778.0 19 21239.0 20 30430.0 21 42779.0 22 56071.0 23 63583.0 24 67298.0 25 73501.0 26 84965.0 27 101705.0 28 122789.0 29 148441.0 30 178126.0 31 216179.0 32 264335.0 33 335110.0 34 446223.0 35 588809.0 36 854576.0 37 1382356.0 38 1450321.0 39 202144.0 40 19.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.53931767316292 20.698318641134623 14.43779664663351 23.324567039068945 2 14.982288667767863 22.014244281943324 40.37591495717713 22.62755209311168 3 16.88158002786074 28.31378914171025 30.949195952129244 23.855434878299768 4 11.923813018457128 18.00262922912689 38.884686034976 31.188871717439987 5 11.643435921079186 39.617377753762185 35.48175561346391 13.257430711694717 6 29.01984527353666 38.79890279652539 18.675437074072775 13.50581485586517 7 26.278152875732076 33.638614883174654 23.351116394194783 16.732115846898484 8 25.75041184430791 36.32576285520924 20.88200819634535 17.0418171041375 9 27.504429492467086 15.341305803282449 20.713882528750087 36.440382175500375 10-11 24.20585442806205 27.008517492841428 29.24305918360674 19.542568895489783 12-13 24.764795013936894 24.814138975974448 29.26893702156886 21.1521289885198 14-15 22.343108764301427 25.902709686315923 27.211671097266198 24.542510452116456 16-17 21.431077749303057 28.933992727664414 27.72659504116702 21.90833448186551 18-19 21.513416668047622 28.087262939350826 29.62281582242971 20.77650457017185 20-21 22.356681225653745 27.245130305129123 29.67188790738987 20.726300561827262 22-23 21.942423955923932 27.14324796310048 29.44522147005354 21.469106610922044 24-25 21.671365070008502 27.21582131656201 29.557906212145895 21.554907401283593 26-27 21.628624040371268 27.29257989203051 29.549133827173208 21.52966224042502 28-29 21.410196998912205 27.835188446865455 29.55284780197602 21.201766752246318 30-31 22.277494454261202 27.269573027124462 29.48639634064467 20.966536177969665 32-33 22.019419926446375 27.07156892968609 29.542934307399875 21.36607683646766 34-35 21.914532127670984 27.576851726835727 29.634161995002508 20.874454150490777 36-37 21.89662713273287 27.535036195462304 29.609798327518998 20.95853834428583 38-39 21.880965109054827 27.794055046827193 29.241379800144202 21.083600043973778 40-41 21.934161198151696 27.650605524946737 29.296941786995696 21.11829148990587 42-43 21.878417142064034 27.467304192858283 28.958461757454568 21.695816907623115 44-45 21.50438298157569 27.706416954917206 29.215258158894713 21.573941904612386 46-47 21.490311993229533 27.621463810757803 29.08116251198271 21.807061684029957 48-49 21.560898174326574 27.88140143936714 29.03550386654018 21.52219651976611 50-51 21.800683414610834 27.74768970615066 28.77986979520793 21.67175708403058 52-53 21.735141210436044 27.906742755411617 28.68691724579577 21.671198788356573 54-55 21.556579052888157 27.8926351692662 28.884804171797224 21.66598160604842 56-57 21.816815999251908 27.8818456596093 28.37111726893892 21.930221072199874 58-59 21.416812631742175 28.249886882615076 28.68860330140005 21.6446971842427 60-61 22.209283167054554 27.994125932009638 28.63628860942338 21.160302291512426 62-63 21.7530240639175 27.940045083312974 28.809414903951946 21.49751594881758 64-65 21.78127021787307 27.688845338195428 28.831910475951624 21.697973967979884 66-67 21.726775610203987 27.820347706717353 28.658861901558986 21.794014781519675 68-69 21.257810290179624 27.747961753707536 28.89317379398998 22.101054162122864 70-71 21.591953178134933 27.59322228511612 28.9231286202268 21.891695916522146 72-73 21.470720406453168 28.311476581459686 28.227962861549376 21.98984015053777 74-75 21.434611467593783 28.25232215984947 28.304678676582324 22.00838769597443 76-77 21.274244239923853 27.7177156160256 28.57447131824834 22.433568825802205 78-79 21.3142024076793 27.78253868374822 28.50006307183123 22.403195836741247 80-81 21.32016454134223 28.14488965903637 28.28419980967111 22.250745989950286 82-83 21.525809611753356 28.106631683625448 28.232697896874022 22.134860807747174 84-85 21.486214945612502 28.235260429495813 27.902688301847366 22.375836323044318 86-87 21.22849465070808 28.407506057282088 27.908583137639564 22.45541615437027 88-89 21.00147471561963 27.891778219937592 28.420308010599012 22.686439053843767 90-91 21.63577109339354 27.756178806332805 28.373922544723563 22.234127555550096 92-93 21.66619229817989 27.892245385119868 27.992240190007887 22.449322126692355 94-95 21.342956327158404 27.850244286301162 27.958464853182146 22.84833453335829 96-97 21.201900991904225 27.727840210835886 28.177470703425612 22.892788093834284 98-99 21.00200141606346 27.674503110670713 28.106595586762523 23.216899886503306 100 20.247630452386478 28.24236748765207 28.289545340461903 23.220456719499545 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3054.0 1 2235.0 2 2084.0 3 2860.5 4 2383.5 5 2119.0 6 3207.0 7 6468.0 8 10338.0 9 12230.0 10 10625.5 11 8051.0 12 7948.5 13 8432.5 14 8837.5 15 9411.5 16 9855.0 17 10416.5 18 11129.0 19 11922.5 20 12926.0 21 14278.0 22 16453.0 23 19437.5 24 22543.5 25 27109.0 26 33919.0 27 41563.0 28 49697.5 29 60441.5 30 74428.5 31 89646.5 32 107848.0 33 128045.0 34 148623.5 35 168360.5 36 191050.0 37 213290.5 38 222815.0 39 228977.5 40 232097.0 41 232792.5 42 238315.5 43 250365.0 44 269745.5 45 289713.5 46 294860.5 47 285001.5 48 279523.5 49 279451.0 50 276744.5 51 263139.0 52 241151.5 53 216076.0 54 188737.0 55 166379.5 56 147768.5 57 127335.0 58 107585.5 59 85679.0 60 64604.0 61 50002.5 62 39380.0 63 30531.5 64 22333.5 65 16244.5 66 11948.0 67 8925.0 68 6749.0 69 5132.0 70 4060.5 71 3054.5 72 2319.5 73 1881.5 74 1491.5 75 1159.5 76 849.0 77 566.5 78 392.0 79 292.0 80 204.0 81 144.0 82 102.0 83 77.5 84 68.0 85 46.0 86 25.0 87 18.5 88 15.0 89 12.5 90 11.0 91 6.0 92 3.5 93 4.0 94 3.5 95 3.5 96 5.0 97 4.5 98 2.5 99 4.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00321068658535196 2 0.0711825491342317 3 9.469306058180896E-4 4 6.510147914999367E-4 5 0.0015831496066021184 6 6.214232100681213E-4 7 0.0 8 8.433600708067359E-4 9 5.91831628636306E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.767790125851517E-4 18-19 0.001050501140829443 20-21 0.0028999749803178994 22-23 0.0 24-25 0.0 26-27 1.5535580251703032E-4 28-29 9.32134815102182E-4 30-31 0.0016497306648237031 32-33 0.003084922364266745 34-35 0.00431297299368708 36-37 0.001435191699443042 38-39 0.001568353815886211 40-41 0.005467044669527877 42-43 0.006998409008624318 44-45 0.005622400472044907 46-47 0.007449680625459501 48-49 0.00843360070806736 50-51 0.008226459638044654 52-53 0.0037729266325564506 54-55 0.0022637559795338704 56-57 0.002411713886692947 58-59 0.0031515034224883294 60-61 0.007405293253311778 62-63 0.014322325412998606 64-65 0.004017057179368927 66-67 0.010556796675800109 68-69 0.012539432631731733 70-71 0.005674185739550583 72-73 0.007516261683681086 74-75 0.003077524468908791 76-77 0.0032254823760678674 78-79 0.007568046951186762 80-81 0.005585410995255138 82-83 0.016556489811100663 84-85 0.01771795938229941 86-87 0.02253398926032735 88-89 0.021365121793770647 90-91 0.024708970495565773 92-93 0.03444460078663301 94-95 0.038017784244524705 96-97 0.02330337037755455 98-99 0.01795469203375393 100 0.018805449999918622 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6758679.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.20953353615791 #Duplication Level Percentage of deduplicated Percentage of total 1 71.96103557775857 36.131300291381216 2 16.426670358268826 16.49550912481819 3 5.456769248631797 8.219455157647602 4 2.3482077197507096 4.716096570187527 5 1.1557843242630939 2.901569589482671 6 0.6839361535950471 2.0604069144312818 7 0.423979812855601 1.4901480042559079 8 0.28516244862672013 1.145429882206097 9 0.19701118870339493 0.8902655897581299 >10 0.8885364510085586 8.08643459791629 >50 0.08256183391867512 2.904493768654127 >100 0.07952636425994951 8.263437869301061 >500 0.00721234717838898 2.476395875570425 >1k 0.0034859654748508047 2.75098845097616 >5k 0.0 0.0 >10k+ 1.2020570602917251E-4 1.4680683134133004 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28767 0.42563051152451536 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25525 0.37766255802354276 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18680 0.2763853705731549 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 7.397895357953825E-6 0.0 14-15 0.0 0.0 0.0 1.479579071590765E-5 0.0 16-17 0.0 0.0 0.0 1.479579071590765E-5 0.0 18-19 0.0 0.0 0.0 1.479579071590765E-5 0.0 20-21 0.0 0.0 0.0 2.95915814318153E-5 0.0 22-23 0.0 0.0 0.0 6.658105822158442E-5 0.0 24-25 0.0 0.0 0.0 1.1096843036930737E-4 0.0 26-27 0.0 0.0 0.0 1.8494738394884562E-4 0.0 28-29 0.0 0.0 0.0 8.803495475965051E-4 0.0 30-31 0.0 0.0 0.0 0.003957874016505296 0.0 32-33 0.0 0.0 0.0 0.011659083084135227 0.0 34-35 0.0 0.0 0.0 0.024035762017991978 0.0 36-37 0.0 0.0 0.0 0.04368457208871734 0.0 38-39 0.0 0.0 0.0 0.07980849512160587 0.0 40-41 0.0 0.0 0.0 0.12611932006239682 0.0 42-43 0.0 1.479579071590765E-5 0.0 0.1690715005106767 0.0 44-45 0.0 1.479579071590765E-5 0.0 0.22196645232004658 0.0 46-47 0.0 1.479579071590765E-5 0.0 0.28433810808295523 0.0 48-49 0.0 1.479579071590765E-5 0.0 0.35367118337769854 0.0 50-51 0.0 1.479579071590765E-5 0.0 0.43423426382581565 0.0 52-53 0.0 1.479579071590765E-5 0.0 0.5188587888254494 7.397895357953825E-6 54-55 0.0 1.479579071590765E-5 0.0 0.6135666451979743 1.479579071590765E-5 56-57 0.0 1.479579071590765E-5 0.0 0.7218274458662706 1.479579071590765E-5 58-59 0.0 1.479579071590765E-5 0.0 0.8447878646108211 1.479579071590765E-5 60-61 0.0 1.479579071590765E-5 0.0 0.9795109369745183 1.479579071590765E-5 62-63 0.0 1.479579071590765E-5 0.0 1.1300729032995944 1.479579071590765E-5 64-65 0.0 1.479579071590765E-5 0.0 1.2797249284956425 1.479579071590765E-5 66-67 0.0 1.479579071590765E-5 0.0 1.4514596713351824 1.479579071590765E-5 68-69 0.0 1.479579071590765E-5 0.0 1.659015023497935 1.479579071590765E-5 70-71 0.0 1.479579071590765E-5 0.0 1.8873880531979697 1.479579071590765E-5 72-73 0.0 1.479579071590765E-5 0.0 2.109199149715499 2.95915814318153E-5 74-75 0.0 1.479579071590765E-5 0.0 2.32850975760204 3.6989476789769125E-5 76-77 1.479579071590765E-5 1.479579071590765E-5 0.0 2.57082042215646 4.438737214772295E-5 78-79 1.479579071590765E-5 1.479579071590765E-5 0.0 2.8538565006564154 4.438737214772295E-5 80-81 2.95915814318153E-5 1.479579071590765E-5 0.0 3.1580801514615504 4.438737214772295E-5 82-83 2.95915814318153E-5 1.479579071590765E-5 0.0 3.4823816902681726 4.438737214772295E-5 84-85 2.95915814318153E-5 1.479579071590765E-5 0.0 3.809390858775805 4.438737214772295E-5 86-87 2.95915814318153E-5 1.479579071590765E-5 0.0 4.135386515619398 4.438737214772295E-5 88 2.95915814318153E-5 1.479579071590765E-5 0.0 4.400564074725254 4.438737214772295E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15460 0.0 48.553867 1 TACCTGG 4985 0.0 36.700504 2 GTATCAA 25065 0.0 34.95792 1 ACCTGGG 5775 0.0 30.758884 3 GTACCTG 6375 0.0 29.584528 1 GTACATG 33555 0.0 28.663984 1 TACATGG 35090 0.0 27.074196 2 TCAACGC 31915 0.0 26.930426 4 ATCAACG 31970 0.0 26.855095 3 CAACGCA 32200 0.0 26.750246 5 ATGGGGG 12055 0.0 26.702045 5 ACATGGG 34935 0.0 26.176577 3 TATCAAC 33070 0.0 26.054268 2 AACGCAG 33450 0.0 25.792181 6 CATGGGG 19920 0.0 24.769903 4 TGGGGGG 11325 0.0 24.27332 6 CGCAGAG 38075 0.0 22.276594 8 TATAACG 425 0.0 22.132406 2 ACGCAGA 39085 0.0 21.953419 7 GAGTACT 21865 0.0 21.050852 12-13 >>END_MODULE