FastQCFastQC Report
Mon 6 Jun 2016
ERR1211470_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211470_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6393572
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165220.25841579636547457No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143040.2237247034990769No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93560.14633447468801478No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95350.046.3596951
GTATCAA162600.036.218661
TCAACGC191050.029.7355294
TACCTGG58600.029.6890472
ATCAACG192200.029.5338553
CAACGCA194900.029.0492045
AACGCAG202200.028.1102526
TATCAAC205050.027.8846992
GTACCTG64850.027.7299461
GTACATG214550.027.6244011
TACATGG215200.026.8972532
ACATGGG216050.025.7733383
ACGCAGA230500.024.4155657
CATGGGG129400.024.2933984
CGCAGAG233700.024.0211328
ACCTGGG73650.023.1601053
ATGGGGG82050.020.90135
GCAGAGT264150.020.7564039
GAGTACT152800.019.58763112-13
AGAGTAC243000.018.70581210-11
Produced by FastQC (version 0.11.5)