##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211470_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6393572 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.508834341741988 31.0 31.0 34.0 30.0 34.0 2 31.566065729767335 33.0 31.0 34.0 30.0 34.0 3 31.668941712082074 33.0 31.0 34.0 28.0 34.0 4 35.20826448814528 37.0 35.0 37.0 32.0 37.0 5 35.286511827817066 37.0 35.0 37.0 33.0 37.0 6 35.395394155254685 37.0 35.0 37.0 33.0 37.0 7 35.331987658854864 37.0 35.0 37.0 33.0 37.0 8 35.29841518950596 37.0 35.0 37.0 32.0 37.0 9 36.90083821688408 39.0 37.0 39.0 33.0 39.0 10-11 36.886809830248254 39.0 37.0 39.0 33.0 39.0 12-13 36.83316845419118 39.0 37.0 39.0 33.0 39.0 14-15 38.06150755790347 40.0 38.0 41.0 33.0 41.0 16-17 38.0470266855523 40.0 38.0 41.0 33.0 41.0 18-19 38.04684963272487 40.0 38.0 41.0 33.0 41.0 20-21 37.99725654141378 40.0 38.0 41.0 33.0 41.0 22-23 37.856739628489365 40.0 38.0 41.0 32.0 41.0 24-25 37.78085208080866 40.0 38.0 41.0 32.0 41.0 26-27 37.64574028414789 40.0 37.0 41.0 32.0 41.0 28-29 37.49087466286451 40.0 37.0 41.0 31.5 41.0 30-31 37.324322069103154 40.0 37.0 41.0 31.0 41.0 32-33 37.177310038895314 40.0 36.5 41.0 31.0 41.0 34-35 37.01856763324163 40.0 36.0 41.0 30.0 41.0 36-37 36.8587439853653 39.0 36.0 41.0 30.0 41.0 38-39 36.73808662825725 39.0 36.0 41.0 30.0 41.0 40-41 36.70169499928991 39.0 36.0 41.0 30.0 41.0 42-43 36.647375207474006 39.0 35.5 41.0 30.0 41.0 44-45 36.472188707658255 39.0 35.0 41.0 29.5 41.0 46-47 36.43505297195371 39.0 35.0 41.0 29.0 41.0 48-49 36.415260592983074 39.0 35.0 41.0 29.0 41.0 50-51 35.45480343382385 38.0 34.0 40.0 27.0 40.5 52-53 35.667384366673275 38.5 34.5 39.5 28.0 40.5 54-55 36.25772494624289 39.0 35.0 40.5 28.0 41.0 56-57 36.22424506989208 39.0 35.0 41.0 28.0 41.0 58-59 36.06434869271825 39.0 35.0 41.0 28.0 41.0 60-61 35.87447095301343 39.0 35.0 40.5 28.0 41.0 62-63 35.62289929635578 38.0 34.5 40.0 28.0 41.0 64-65 35.341164610330495 38.0 34.0 40.0 27.0 41.0 66-67 35.03616350922458 37.0 34.0 40.0 26.0 41.0 68-69 34.74820155305986 37.0 34.0 39.5 26.0 41.0 70-71 34.39544764647994 36.0 34.0 39.0 26.0 41.0 72-73 34.04290137031381 36.0 33.0 39.0 26.0 40.5 74-75 33.66922606017418 35.0 33.0 38.0 26.0 40.0 76-77 33.31743585588775 35.0 33.0 37.0 25.0 39.0 78-79 32.99028571196195 35.0 32.5 37.0 25.0 39.0 80-81 32.63536916452962 35.0 32.0 36.0 24.5 39.0 82-83 32.32572754322623 35.0 32.0 36.0 24.0 37.5 84-85 32.05717797500365 35.0 32.0 35.5 24.0 37.0 86-87 31.760499138822553 35.0 31.0 35.0 23.0 37.0 88-89 31.531022329927623 34.5 31.0 35.0 22.5 36.0 90-91 31.29711794909012 34.0 31.0 35.0 20.5 36.0 92-93 31.142556617803006 34.0 31.0 35.0 20.0 36.0 94-95 30.979161179384544 34.0 31.0 35.0 20.0 35.0 96-97 30.879064785694133 34.0 31.0 35.0 20.0 35.0 98-99 30.702452713444067 34.0 30.0 35.0 19.0 35.0 100 29.315654691931208 33.0 27.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3688688962202811 1101 2 0.3751853597172392 1101 3 0.3484196883823749 1101 4 0.32161464695909103 1101 5 0.27484222370821243 1101 6 0.34020834927359545 1101 7 0.38111435332257315 1101 8 0.37714363659503647 1101 9 0.3914658775005293 1101 10-11 0.3920859033185593 1101 12-13 0.3571937144057884 1101 14-15 0.43809395573378396 1101 16-17 0.4384007857060581 1101 18-19 0.4337820545787636 1101 20-21 0.3974848841455909 1101 22-23 0.4608195392659553 1101 24-25 0.42638905217815903 1101 26-27 0.4195035637757556 1101 28-29 0.45195773100353875 1101 30-31 0.5046516742563014 1101 32-33 0.40580994544537674 1101 34-35 0.36719031572575034 1101 36-37 0.42593281208630884 1101 38-39 0.33678839214991996 1101 40-41 0.26881603752050864 1101 42-43 0.2635862826574211 1101 44-45 0.22530515005824725 1101 46-47 0.15347092986447564 1101 48-49 0.24361371010478905 1101 50-51 0.23169300053795183 1101 52-53 0.3185642346328237 1101 54-55 0.3543689476604541 1101 56-57 0.31782186540500135 1101 58-59 0.3247662187449336 1101 60-61 0.2699042095330597 1101 62-63 0.23309513005391835 1101 64-65 0.2260585183013717 1101 66-67 0.1782972405165566 1101 68-69 0.1700859693721668 1101 70-71 0.15332621861740847 1101 72-73 0.1372005215408194 1101 74-75 0.035313037687480175 1101 76-77 0.025184442707121946 1101 78-79 0.024795251018296938 1101 80-81 0.08639728063985075 1101 82-83 -0.05911274448349957 1101 84-85 -0.16027839422138257 1101 86-87 -0.11919931929428174 1101 88-89 -0.08060182983989606 1101 90-91 -0.19736999713397552 1101 92-93 -0.22988099718435961 1101 94-95 -0.2926566632193115 1101 96-97 -0.2234386864323703 1101 98-99 -0.25542108552209797 1101 100 -0.18330084648711775 1102 1 0.4089053504758695 1102 2 0.3876244688437289 1102 3 0.3310415133509039 1102 4 0.29207468023553673 1102 5 0.3023330381084648 1102 6 0.3037554520740997 1102 7 0.34011792877045366 1102 8 0.3060432663719439 1102 9 0.3870803350541294 1102 10-11 0.36611815315040275 1102 12-13 0.38945612165448296 1102 14-15 0.3904457203650935 1102 16-17 0.4327723573142208 1102 18-19 0.4448406481261671 1102 20-21 0.435174410462551 1102 22-23 0.43887814022275506 1102 24-25 0.48640429908000726 1102 26-27 0.3798610632838546 1102 28-29 0.3495585050390204 1102 30-31 0.3316528301229411 1102 32-33 0.2924544168380905 1102 34-35 0.323490077060697 1102 36-37 0.31644877811173444 1102 38-39 0.2869414786184663 1102 40-41 0.3520729511095979 1102 42-43 0.3030219167745898 1102 44-45 0.22778283335243543 1102 46-47 0.14779036810011803 1102 48-49 0.23577596282630964 1102 50-51 0.1806381693199981 1102 52-53 0.2673963482963444 1102 54-55 0.3194695549844013 1102 56-57 0.3508887072628468 1102 58-59 0.3210746284980175 1102 60-61 0.2409274808167723 1102 62-63 0.26357928093238314 1102 64-65 0.30531437563910657 1102 66-67 0.23166625423464637 1102 68-69 0.1754228378133007 1102 70-71 0.24571585763960258 1102 72-73 0.24707542202843769 1102 74-75 0.1843857716660935 1102 76-77 0.1187596271394753 1102 78-79 0.11429898988464515 1102 80-81 0.22309573775547165 1102 82-83 0.1165844596200003 1102 84-85 0.051409257516986884 1102 86-87 -0.02108801691084139 1102 88-89 0.003987911596691873 1102 90-91 -0.0845661807982232 1102 92-93 -0.06736734124568144 1102 94-95 -0.133457623642407 1102 96-97 -0.33273804107349747 1102 98-99 -0.22275328110066184 1102 100 -0.15065852217431797 1103 1 0.33455861716316093 1103 2 0.34965614188989136 1103 3 0.2936218636874699 1103 4 0.35192933686485617 1103 5 0.3273700917710656 1103 6 0.3117888681083798 1103 7 0.3011630543687218 1103 8 0.3639258391194531 1103 9 0.3135349314270144 1103 10-11 0.31824669144660334 1103 12-13 0.2737096293675094 1103 14-15 0.37936040696086337 1103 16-17 0.36568136807747464 1103 18-19 0.39399832182342465 1103 20-21 0.3494035224080889 1103 22-23 0.41718900882531784 1103 24-25 0.3521672203442563 1103 26-27 0.3855256229973705 1103 28-29 0.4145543194411161 1103 30-31 0.3856004858848081 1103 32-33 0.3271749624621165 1103 34-35 0.40923619527249144 1103 36-37 0.37775514972408075 1103 38-39 0.3083957009161722 1103 40-41 0.2715341109852858 1103 42-43 0.2989503612486857 1103 44-45 0.2996585979317672 1103 46-47 0.20987335010609343 1103 48-49 0.2776546896766092 1103 50-51 0.27531593894226347 1103 52-53 0.3077062847440004 1103 54-55 0.3477725882285654 1103 56-57 0.3146997649250167 1103 58-59 0.37622488539667387 1103 60-61 0.28263403198520365 1103 62-63 0.3105344606603424 1103 64-65 0.29307220948098234 1103 66-67 0.2956605076311476 1103 68-69 0.22507231926422833 1103 70-71 0.26182080493543936 1103 72-73 0.21255854517196582 1103 74-75 0.31807998644186597 1103 76-77 0.22232678778757986 1103 78-79 0.1584700392969367 1103 80-81 0.11087394352917102 1103 82-83 0.14133832826501447 1103 84-85 0.12044751024217248 1103 86-87 0.11677930974575546 1103 88-89 0.15523696882614857 1103 90-91 0.06673074794349176 1103 92-93 -0.06802581465500523 1103 94-95 -0.07942393059029484 1103 96-97 0.048878539960284684 1103 98-99 -0.08808429718993693 1103 100 -0.15259249082884097 1104 1 0.48105345688294676 1104 2 0.43503590055675545 1104 3 0.44958024459399226 1104 4 0.42998197971716223 1104 5 0.35872090193414863 1104 6 0.3602141979779958 1104 7 0.35185161721384617 1104 8 0.37879290166831225 1104 9 0.40276521497035844 1104 10-11 0.46072225494611274 1104 12-13 0.4593212075485482 1104 14-15 0.5112742131844428 1104 16-17 0.5364166487638897 1104 18-19 0.4290958857708418 1104 20-21 0.458269172050926 1104 22-23 0.49698156427923124 1104 24-25 0.5444268101946861 1104 26-27 0.5783365940315832 1104 28-29 0.5907199821633995 1104 30-31 0.4706575706646703 1104 32-33 0.4708894325847268 1104 34-35 0.5020824381039688 1104 36-37 0.5288077708112056 1104 38-39 0.5596118761474358 1104 40-41 0.5069996793850109 1104 42-43 0.301498840137171 1104 44-45 0.4642476865789007 1104 46-47 0.532368813489839 1104 48-49 0.48285526598604633 1104 50-51 0.5043788014621953 1104 52-53 0.6345019375661849 1104 54-55 0.6629966811036425 1104 56-57 0.6380861768210977 1104 58-59 0.6281998314363122 1104 60-61 0.6434922737369604 1104 62-63 0.656779061827649 1104 64-65 0.5800241668114623 1104 66-67 0.5501906960382854 1104 68-69 0.3940615951326052 1104 70-71 0.43132231517453334 1104 72-73 0.6091438426094271 1104 74-75 0.5802293814206934 1104 76-77 0.5402381481744172 1104 78-79 0.4722553215191212 1104 80-81 0.3849373033936416 1104 82-83 0.477834325404217 1104 84-85 0.33080313905350067 1104 86-87 0.34806907569683077 1104 88-89 0.4548492943461895 1104 90-91 0.40469796958158 1104 92-93 0.39216080854639657 1104 94-95 0.3981228650564219 1104 96-97 0.49637259470967976 1104 98-99 0.44107469826764856 1104 100 0.3801842641210591 1105 1 0.353740878542002 1105 2 0.39981867748113586 1105 3 0.3551749175256198 1105 4 0.3487460405410161 1105 5 0.28374992138934374 1105 6 0.2818188058736055 1105 7 0.29541022492873736 1105 8 0.3058990422082033 1105 9 0.3847986388953686 1105 10-11 0.387231208693386 1105 12-13 0.3823901105088794 1105 14-15 0.44981900045619483 1105 16-17 0.48141001078072065 1105 18-19 0.4150362203808058 1105 20-21 0.4683460475418215 1105 22-23 0.501098735687485 1105 24-25 0.4924686630840043 1105 26-27 0.5036222126614902 1105 28-29 0.5020513553802388 1105 30-31 0.48813817223043543 1105 32-33 0.4823298170085195 1105 34-35 0.4748887178909058 1105 36-37 0.4994396187654786 1105 38-39 0.5030812823433521 1105 40-41 0.5686935351380455 1105 42-43 0.5723291325096298 1105 44-45 0.484144838385987 1105 46-47 0.4289460237760423 1105 48-49 0.5015348749821982 1105 50-51 0.5304123226979485 1105 52-53 0.5337343411184889 1105 54-55 0.5822165964432884 1105 56-57 0.5682454420786129 1105 58-59 0.5489940956627919 1105 60-61 0.5738710674080565 1105 62-63 0.6209316358127737 1105 64-65 0.5941539191092744 1105 66-67 0.4169462166855382 1105 68-69 0.47263770719752074 1105 70-71 0.42733315373831715 1105 72-73 0.4599102805958353 1105 74-75 0.44901973931572314 1105 76-77 0.49861637188256225 1105 78-79 0.49258747553886906 1105 80-81 0.533218610230648 1105 82-83 0.4943308141080607 1105 84-85 0.41907788899962384 1105 86-87 0.5196088097604594 1105 88-89 0.47777292626133416 1105 90-91 0.35806758592237387 1105 92-93 0.44465532323220813 1105 94-95 0.48465297230676896 1105 96-97 0.4690572360616514 1105 98-99 0.5114367405885574 1105 100 0.48637925169076723 1106 1 0.38475813804149084 1106 2 0.3745779366797919 1106 3 0.35766289669674833 1106 4 0.35880189068581103 1106 5 0.3588607847570273 1106 6 0.32568987699185215 1106 7 0.3579398671758298 1106 8 0.32472439169431055 1106 9 0.4454160872810391 1106 10-11 0.4515847771139079 1106 12-13 0.406004732025643 1106 14-15 0.5328530175444328 1106 16-17 0.4862037977051301 1106 18-19 0.4777083393422501 1106 20-21 0.42185618653874 1106 22-23 0.5063012265513365 1106 24-25 0.5079163872199999 1106 26-27 0.5377507490808071 1106 28-29 0.5662684770451776 1106 30-31 0.6462352372343645 1106 32-33 0.6184348648294318 1106 34-35 0.5478644001277075 1106 36-37 0.6156550748285312 1106 38-39 0.6056085804818494 1106 40-41 0.5586756222213154 1106 42-43 0.4379481680774191 1106 44-45 0.49425889732880535 1106 46-47 0.4738547629256047 1106 48-49 0.5532270450413321 1106 50-51 0.5904780753077077 1106 52-53 0.6466176237819852 1106 54-55 0.6413859759853793 1106 56-57 0.6559459503283804 1106 58-59 0.5617895166757236 1106 60-61 0.49493233166799655 1106 62-63 0.5767405829625787 1106 64-65 0.6202064317476399 1106 66-67 0.6340175208571495 1106 68-69 0.5800413936052351 1106 70-71 0.5823051850320482 1106 72-73 0.433422217065889 1106 74-75 0.511790519311198 1106 76-77 0.4841110484895168 1106 78-79 0.46527464091403203 1106 80-81 0.43856625644382063 1106 82-83 0.44747967070060923 1106 84-85 0.5023890114797922 1106 86-87 0.5380809369881128 1106 88-89 0.5392666579525276 1106 90-91 0.5567995734658702 1106 92-93 0.42961381832903456 1106 94-95 0.4630567507092387 1106 96-97 0.521836556175618 1106 98-99 0.4836718105621358 1106 100 0.5462135932695702 1107 1 0.2111966768323832 1107 2 0.20495469403902788 1107 3 0.23637266331486018 1107 4 0.2349572931713979 1107 5 0.20233253332208534 1107 6 0.1628167715049429 1107 7 0.17680671181124552 1107 8 0.21229878116319156 1107 9 0.08443444674114176 1107 10-11 0.21610770407225033 1107 12-13 0.23549723347678508 1107 14-15 0.2648334144806981 1107 16-17 0.23409742157352298 1107 18-19 0.22872771529707592 1107 20-21 0.30101480912264833 1107 22-23 0.33832878120050935 1107 24-25 0.2878301187016561 1107 26-27 0.25811703642114736 1107 28-29 0.3170523315015359 1107 30-31 0.343907478700757 1107 32-33 0.3365217634183608 1107 34-35 0.3817012352627671 1107 36-37 0.3542711094575566 1107 38-39 0.300361830394003 1107 40-41 0.3556941371960747 1107 42-43 0.3510787920660974 1107 44-45 0.3884503325801134 1107 46-47 0.3710524895355647 1107 48-49 0.4294226204337406 1107 50-51 0.35453773500179153 1107 52-53 0.39592404794440483 1107 54-55 0.438226306502294 1107 56-57 0.41464777070238057 1107 58-59 0.418514503391755 1107 60-61 0.3898277531995049 1107 62-63 0.41961879762971677 1107 64-65 0.4487692144204374 1107 66-67 0.47830679988853575 1107 68-69 0.36532307183226465 1107 70-71 0.4680943505089843 1107 72-73 0.4046453500862839 1107 74-75 0.36351169365843106 1107 76-77 0.3936302503891298 1107 78-79 0.4261925142829881 1107 80-81 0.5116005907717707 1107 82-83 0.4204412295121074 1107 84-85 0.33616940321853406 1107 86-87 0.3930161185011336 1107 88-89 0.3919033760304522 1107 90-91 0.4481884990763483 1107 92-93 0.39395562310226495 1107 94-95 0.3717656534711864 1107 96-97 0.32725052046702885 1107 98-99 0.3737763125059175 1107 100 0.6332211246367443 1108 1 0.3542339064330271 1108 2 0.3481112871248797 1108 3 0.32023776394502335 1108 4 0.29497519364383606 1108 5 0.2378870960945889 1108 6 0.23195743238050426 1108 7 0.28090120945632435 1108 8 0.26381830121424343 1108 9 0.237206523948295 1108 10-11 0.27927567455986235 1108 12-13 0.3121946621293077 1108 14-15 0.3507365544754961 1108 16-17 0.34589038809939865 1108 18-19 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100 -0.0019850292228902333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 11.0 1 48.0 2 85.0 3 83.0 4 93.0 5 95.0 6 201.0 7 1373.0 8 2569.0 9 3358.0 10 5310.0 11 8656.0 12 12434.0 13 14679.0 14 15599.0 15 16634.0 16 18005.0 17 20962.0 18 25231.0 19 29736.0 20 36009.0 21 43322.0 22 50268.0 23 53959.0 24 54818.0 25 56906.0 26 61968.0 27 70472.0 28 82885.0 29 99754.0 30 122550.0 31 152676.0 32 196467.0 33 261539.0 34 366105.0 35 526160.0 36 826333.0 37 1344043.0 38 1448143.0 39 363504.0 40 529.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.00519903037488 18.71950143949289 12.729650733813969 24.545648796318257 2 15.430450657557932 20.143165740419217 40.85999014939048 23.566393452632376 3 18.138384120946547 25.790413716754625 30.263469152884763 25.807733009414058 4 11.998130672676217 16.524912995235624 38.69998829714732 32.77696803494084 5 12.649104189771998 37.848484720482396 35.69683176721622 13.805579322529384 6 30.66888268878184 37.233332050768304 18.455985652789625 13.641799607660229 7 26.720752828824946 32.46499229906439 23.02233294788249 17.791921924228173 8 25.26169731142027 35.8685328808571 20.842061269486134 18.027708538236496 9 26.352563461864225 15.13463655177516 20.458111360905857 38.05468862545476 10-11 24.4438883064647 25.986597682964074 29.4235017219205 20.14601228865073 12-13 24.99417540238995 23.82256903116001 29.11543308376135 22.067822482688687 14-15 22.367357716722143 25.266069862560688 26.600165756901767 25.766406663815406 16-17 22.060361846980513 27.995100625889492 27.29756314717135 22.64697437995865 18-19 21.862919535108983 27.312495854550733 28.966592684736863 21.85799192560342 20-21 22.637592340151535 26.657069850782133 28.87375754763705 21.831580261429277 22-23 22.305743489054827 26.624978445760146 28.610253027216707 22.45902503796832 24-25 22.03748706513229 26.685873755691148 28.77742328831517 22.499215890861393 26-27 22.0204866253958 26.852875677943338 28.637446701878872 22.48919099478199 28-29 21.990108710318975 26.96522012910695 28.707158767128377 22.3375123934457 30-31 22.405424056305417 26.792908449652742 28.710805040087482 22.09086245395436 32-33 22.112492265180016 26.92724806857311 28.671291093687707 22.288968572559163 34-35 22.294451935184266 27.041948820908534 28.635708661793018 22.02789058211418 36-37 22.265815475777963 26.99675813036906 28.51888655396126 22.21853983989172 38-39 22.15351234489925 27.157315679083855 28.516480658530202 22.172691317486695 40-41 22.350850803283297 26.93192595956147 28.5546297958518 22.162593441303432 42-43 22.11040125842784 26.990978753391044 28.440395608707675 22.458224379473446 44-45 22.094364908803975 27.18576198731743 28.379152543128665 22.34072056074993 46-47 22.09533608513303 27.039932331101717 28.186475559702558 22.678256024062694 48-49 22.065242521501553 27.135404331814854 28.296797925914706 22.502555220768887 50-51 22.16364154979275 27.31016870529753 28.134005360796326 22.392184384113392 52-53 22.162123940542905 27.37421716447599 27.996402550753587 22.467256344227522 54-55 22.08616248279513 27.287453579316672 28.140657056241107 22.485726881647086 56-57 22.140851072283997 27.39996179828399 27.90030728759525 22.55887984183676 58-59 22.143592984772596 27.332846273404886 28.110056249164007 22.41350449265851 60-61 22.31926308980076 27.421440075593367 28.032898447222703 22.226398387383167 62-63 22.170518483895194 27.40431315794765 28.14943067163229 22.27573768652486 64-65 22.10032019880926 27.193422860499016 28.131896689026032 22.574360251665688 66-67 22.067587839998897 27.308949014352145 27.978627785833886 22.644835359815065 68-69 22.019783693053554 27.379373673093387 28.040287663535945 22.560554970317106 70-71 22.04094731243852 27.17734734179188 28.063627947090726 22.71807739867888 72-73 21.996960365288146 27.37796027790275 27.884897246280588 22.740182110528515 74-75 22.06665364343642 27.391303293377785 27.87036466948364 22.67167839370216 76-77 21.954404395705478 27.275286484125516 27.96048458138064 22.809824538788366 78-79 22.025413957231056 27.179373306350175 27.929643656780335 22.86556907963844 80-81 22.026754111060605 27.382285204421375 27.76498740296048 22.82597328155754 82-83 22.145023241691298 27.37170335328145 27.82281015430662 22.660463250720632 84-85 22.101884623055835 27.41218063284852 27.71192134563416 22.774013398461484 86-87 21.90155329854582 27.423481759082602 27.90967365274519 22.765291289626383 88-89 21.974839616024873 27.24949568243468 27.88717553145267 22.888489170087773 90-91 22.223225238589695 27.24109883380834 27.8254462610166 22.710229666585366 92-93 22.084577284885363 27.23813890924007 27.88087915244365 22.796404653430912 94-95 22.01085451668726 27.16274516368128 27.85829400775488 22.96810631187658 96-97 21.986262139981072 27.100366406025934 27.91948687670129 22.993884577291713 98-99 21.98230678511576 27.020322386819444 27.815112286932187 23.18225854113261 100 22.12229661445372 27.039902841391733 27.76172358269552 23.076076961459034 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 897.0 1 703.0 2 941.5 3 1459.5 4 1224.5 5 1118.0 6 1754.0 7 3343.5 8 5414.0 9 6400.0 10 5599.0 11 4713.0 12 4826.0 13 5091.5 14 5407.5 15 5686.5 16 5968.0 17 6487.0 18 7161.5 19 7864.0 20 8997.5 21 10793.0 22 13177.5 23 16363.5 24 20786.5 25 26377.0 26 33541.0 27 42178.0 28 51324.5 29 61897.5 30 74679.0 31 87694.5 32 101356.5 33 116976.0 34 133089.5 35 147471.0 36 163274.5 37 179123.5 38 190796.5 39 200277.5 40 206332.5 41 210232.0 42 214755.0 43 221651.5 44 228358.5 45 235490.5 46 240495.5 47 241751.5 48 245048.5 49 246515.5 50 244752.5 51 237802.0 52 226302.5 53 215724.0 54 205045.5 55 190781.0 56 173247.0 57 155025.0 58 136977.5 59 117526.5 60 96852.5 61 78729.0 62 63004.0 63 48785.0 64 36440.0 65 27084.0 66 20347.0 67 15290.0 68 11791.5 69 9045.0 70 6906.0 71 5323.5 72 4136.0 73 3257.5 74 2621.5 75 1994.0 76 1485.5 77 1100.0 78 778.5 79 586.5 80 472.5 81 374.0 82 301.5 83 253.5 84 220.5 85 172.0 86 126.0 87 94.5 88 80.5 89 68.5 90 52.0 91 44.0 92 33.0 93 22.0 94 17.0 95 16.5 96 17.0 97 12.0 98 9.5 99 10.5 100 11.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1575645038485529 2 0.09619036119402424 3 0.02902915615871691 4 0.030780915582087762 5 0.025791529367308292 6 0.002095855024390122 7 0.003018656863487265 8 0.004817338414269833 9 0.0050519490513284285 10-11 0.004293374658172301 12-13 0.006670762447032739 14-15 0.016860684449944412 16-17 0.007984582014560874 18-19 0.015953523319984512 20-21 0.018362192527119427 22-23 0.042511447435017544 24-25 0.015124565735710806 26-27 0.00818009087877637 28-29 0.02478270362795633 30-31 0.013537033758280975 32-33 0.018964359828903155 34-35 0.019316275784491046 36-37 0.019910622731706157 38-39 0.01805719869894325 40-41 0.023844261079721946 42-43 0.0329236927338896 44-45 0.03444866187477047 46-47 0.01987152095886306 48-49 0.0121684717087725 50-51 0.010612221149617147 52-53 0.01978549705860824 54-55 0.023242093777938216 56-57 0.01859680316417802 58-59 0.02310132739570306 60-61 0.02310132739570306 62-63 0.04854094080742346 64-65 0.029998880125225773 66-67 0.019605628903529983 68-69 0.02487654788277977 70-71 0.03880459936949173 72-73 0.036654001863121274 74-75 0.03798346213978665 76-77 0.02084906527994054 78-79 0.014749188716417052 80-81 0.021826609601018024 82-83 0.016774660549689596 84-85 0.01637582246668998 86-87 0.019394479330177246 88-89 0.015992625092827607 90-91 0.027230474607934346 92-93 0.025697685112484853 94-95 0.018925258056060053 96-97 0.012520387664360392 98-99 0.016657355231160298 100 0.037115402782669844 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6393572.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.97422109000795 #Duplication Level Percentage of deduplicated Percentage of total 1 75.52608130171446 48.31722223257796 2 15.490031747862712 19.819254314580228 3 4.5536925094275045 8.739567941220848 4 1.806067016176549 4.621669223849981 5 0.8838280164659561 2.827110446546813 6 0.4830021315584587 1.853981109075957 7 0.3058641539964527 1.3697194707894116 8 0.19262739026755457 0.9858549802374226 9 0.1327945017800915 0.7645882334775325 >10 0.5826224534816649 6.2842519639293215 >50 0.02750413636582125 1.20416028315038 >100 0.0146286491586473 1.715970700992351 >500 9.01738764576383E-4 0.3732618957215857 >1k 2.57638530580965E-4 0.2955262819964072 >5k 3.2204816322124506E-5 0.1927596133807368 >10k+ 6.440963264424901E-5 0.6351013084731287 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16522 0.25841579636547457 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14304 0.2237247034990769 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9356 0.14633447468801478 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 1.5640709137239716E-5 0.0 3 0.0 1.5640709137239716E-5 0.0 1.5640709137239716E-5 0.0 4 0.0 1.5640709137239716E-5 0.0 1.5640709137239716E-5 0.0 5 0.0 3.128141827447943E-5 0.0 3.128141827447943E-5 0.0 6 0.0 3.128141827447943E-5 0.0 3.128141827447943E-5 0.0 7 0.0 3.128141827447943E-5 0.0 3.128141827447943E-5 0.0 8 0.0 3.128141827447943E-5 0.0 4.6922127411719144E-5 0.0 9 0.0 3.128141827447943E-5 0.0 6.256283654895886E-5 4.6922127411719144E-5 10-11 0.0 3.128141827447943E-5 0.0 6.256283654895886E-5 4.6922127411719144E-5 12-13 0.0 3.128141827447943E-5 0.0 6.256283654895886E-5 4.6922127411719144E-5 14-15 0.0 3.910177284309929E-5 0.0 8.602390025481843E-5 4.6922127411719144E-5 16-17 6.256283654895886E-5 4.6922127411719144E-5 0.0 1.09484963960678E-4 4.6922127411719144E-5 18-19 6.256283654895886E-5 4.6922127411719144E-5 0.0 1.09484963960678E-4 5.474248198033901E-5 20-21 6.256283654895886E-5 4.6922127411719144E-5 0.0 1.09484963960678E-4 6.256283654895886E-5 22-23 6.256283654895886E-5 4.6922127411719144E-5 0.0 1.2512567309791773E-4 7.038319111757872E-5 24-25 6.256283654895886E-5 4.6922127411719144E-5 0.0 1.5640709137239715E-4 7.820354568619858E-5 26-27 6.256283654895886E-5 4.6922127411719144E-5 0.0 2.1114957335273616E-4 7.820354568619858E-5 28-29 6.256283654895886E-5 4.6922127411719144E-5 0.0 8.21137229705085E-4 7.820354568619858E-5 30-31 6.256283654895886E-5 4.6922127411719144E-5 0.0 0.003065578990898984 7.820354568619858E-5 32-33 6.256283654895886E-5 4.6922127411719144E-5 0.0 0.008649312152893562 7.820354568619858E-5 34-35 6.256283654895886E-5 5.474248198033901E-5 0.0 0.017986815507825674 7.820354568619858E-5 36-37 7.038319111757872E-5 6.256283654895886E-5 0.0 0.03332253081688921 7.820354568619858E-5 38-39 7.820354568619858E-5 6.256283654895886E-5 0.0 0.05974750890425572 7.820354568619858E-5 40-41 7.820354568619858E-5 7.038319111757872E-5 0.0 0.09188916618128333 7.820354568619858E-5 42-43 7.820354568619858E-5 1.09484963960678E-4 0.0 0.12375711104840925 7.820354568619858E-5 44-45 7.820354568619858E-5 1.2512567309791773E-4 0.0 0.16213159091662688 9.384425482343829E-5 46-47 7.820354568619858E-5 1.2512567309791773E-4 0.0 0.21006254406769798 1.09484963960678E-4 48-49 7.820354568619858E-5 1.2512567309791773E-4 0.0 0.2657669296599773 1.2512567309791773E-4 50-51 1.3294602766653757E-4 1.3294602766653757E-4 0.0 0.32602276161119326 1.2512567309791773E-4 52-53 1.4076638223515744E-4 1.4076638223515744E-4 0.0 0.3874516467477022 1.485867368037773E-4 54-55 1.4076638223515744E-4 1.64227445941017E-4 0.0 0.45736561659116376 1.7986815507825673E-4 56-57 1.4076638223515744E-4 1.7204780050963686E-4 0.0 0.5409495662205728 1.8768850964687658E-4 58-59 1.4076638223515744E-4 1.7204780050963686E-4 0.0 0.6407842126435739 1.8768850964687658E-4 60-61 1.485867368037773E-4 2.033292187841163E-4 0.0 0.7487285667542338 2.033292187841163E-4 62-63 1.5640709137239715E-4 2.033292187841163E-4 0.0 0.8605518167309292 2.033292187841163E-4 64-65 1.5640709137239715E-4 2.033292187841163E-4 0.0 0.9755423103079155 2.033292187841163E-4 66-67 1.5640709137239715E-4 2.5025134619583545E-4 0.0 1.1094502415863934 2.1114957335273616E-4 68-69 1.5640709137239715E-4 2.5025134619583545E-4 0.0 1.2653177284935557 2.18969927921356E-4 70-71 1.64227445941017E-4 2.5025134619583545E-4 0.0 1.4380928219780742 2.18969927921356E-4 72-73 1.7204780050963686E-4 2.5025134619583545E-4 0.0 1.6160606308961563 2.815327644703149E-4 74-75 1.7204780050963686E-4 2.5025134619583545E-4 0.0 1.7941692061964736 2.815327644703149E-4 76-77 1.7986815507825673E-4 2.5025134619583545E-4 0.0 1.9941356725160835 2.815327644703149E-4 78-79 1.8768850964687658E-4 2.5025134619583545E-4 0.0 2.2251176650548397 2.815327644703149E-4 80-81 1.8768850964687658E-4 2.5807170076445527E-4 0.0 2.4783407459867504 2.815327644703149E-4 82-83 1.8768850964687658E-4 2.6589205533307514E-4 0.0 2.7481507989587044 2.815327644703149E-4 84-85 1.8768850964687658E-4 2.6589205533307514E-4 0.0 3.019086982988539 2.815327644703149E-4 86-87 1.8768850964687658E-4 2.6589205533307514E-4 0.0 3.304944403535301 2.815327644703149E-4 88 1.8768850964687658E-4 2.6589205533307514E-4 0.0 3.5375530298243296 2.815327644703149E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9535 0.0 46.359695 1 GTATCAA 16260 0.0 36.21866 1 TCAACGC 19105 0.0 29.735529 4 TACCTGG 5860 0.0 29.689047 2 ATCAACG 19220 0.0 29.533855 3 CAACGCA 19490 0.0 29.049204 5 AACGCAG 20220 0.0 28.110252 6 TATCAAC 20505 0.0 27.884699 2 GTACCTG 6485 0.0 27.729946 1 GTACATG 21455 0.0 27.624401 1 TACATGG 21520 0.0 26.897253 2 ACATGGG 21605 0.0 25.773338 3 ACGCAGA 23050 0.0 24.415565 7 CATGGGG 12940 0.0 24.293398 4 CGCAGAG 23370 0.0 24.021132 8 ACCTGGG 7365 0.0 23.160105 3 ATGGGGG 8205 0.0 20.9013 5 GCAGAGT 26415 0.0 20.756403 9 GAGTACT 15280 0.0 19.587631 12-13 AGAGTAC 24300 0.0 18.705812 10-11 >>END_MODULE