FastQCFastQC Report
Mon 6 Jun 2016
ERR1211470_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211470_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6393572
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188340.2945771158907728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169710.2654384747680952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118480.18531112185801615No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104500.046.8922421
GTATCAA176200.036.2979931
TCAACGC216900.029.0743224
ATCAACG217600.028.8946363
CAACGCA219300.028.7775635
AACGCAG228950.027.687556
TATCAAC229250.027.6103382
TACCTGG55550.027.5128332
GTACCTG60350.026.026681
GTACATG219100.024.7048681
ACGCAGA262500.023.9698057
TACATGG222100.023.735212
CGCAGAG267200.023.5305968
ACATGGG222200.023.1574153
CATGGGG127450.022.8227214
ACCTGGG69750.020.8177743
GCAGAGT299400.020.670169
ATGGGGG80350.019.9427535
GAGTACT173950.019.82873712-13
AGAGTAC274300.019.36680410-11
Produced by FastQC (version 0.11.5)