FastQCFastQC Report
Mon 6 Jun 2016
ERR1211469_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211469_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5454051
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177350.32517114343081865No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154790.2838073938069153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97350.17849118022548743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA92650.048.876031
GTATCAA159700.038.2002641
TACCTGG56200.034.6379172
GTACCTG61350.032.7626881
TCAACGC192800.030.513934
CAACGCA194750.030.2061825
ATCAACG194900.030.113653
ACCTGGG66250.029.2229543
TATCAAC203800.029.0475372
AACGCAG203000.028.9735456
GTACATG200900.028.140341
TACATGG206650.027.1224292
ACATGGG208000.025.980493
CATGGGG123950.025.5513734
ACGCAGA229550.025.4596447
CGCAGAG234800.024.7109858
ATGGGGG78500.022.086445
GCAGAGT258650.022.0172549
GAGTACT149300.020.1744212-13
AGAGTAC235050.019.72985610-11
Produced by FastQC (version 0.11.5)