##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211469_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5454051
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.444155729383535	31.0	31.0	34.0	29.0	34.0
2	31.48251473996118	33.0	31.0	34.0	28.0	34.0
3	31.58419640740433	33.0	31.0	34.0	28.0	34.0
4	35.12860055764055	37.0	35.0	37.0	32.0	37.0
5	35.20884879881028	37.0	35.0	37.0	32.0	37.0
6	35.32431123214653	37.0	35.0	37.0	32.0	37.0
7	35.258030040423165	37.0	35.0	37.0	32.0	37.0
8	35.222598945261055	37.0	35.0	37.0	32.0	37.0
9	36.81233343802616	39.0	37.0	39.0	32.0	39.0
10-11	36.80031897391498	39.0	37.0	39.0	32.5	39.0
12-13	36.74281749473923	39.0	37.0	39.0	32.0	39.0
14-15	37.95397641129502	40.0	38.0	41.0	32.5	41.0
16-17	37.937538171168555	40.0	38.0	41.0	32.0	41.0
18-19	37.942253931985604	40.0	38.0	41.0	32.0	41.0
20-21	37.895698628413996	40.0	38.0	41.0	32.0	41.0
22-23	37.74996896802028	40.0	38.0	41.0	32.0	41.0
24-25	37.66904031517124	40.0	37.0	41.0	32.0	41.0
26-27	37.53104353076273	40.0	37.0	41.0	31.5	41.0
28-29	37.37501446172762	40.0	37.0	41.0	31.0	41.0
30-31	37.20488678965415	40.0	36.5	41.0	31.0	41.0
32-33	37.04302315838264	40.0	36.0	41.0	30.0	41.0
34-35	36.87836536548705	39.0	36.0	41.0	30.0	41.0
36-37	36.70776822585634	39.0	35.5	41.0	30.0	41.0
38-39	36.58244697381818	39.0	35.0	41.0	30.0	41.0
40-41	36.5418863886678	39.0	35.0	41.0	30.0	41.0
42-43	36.48333843962955	39.0	35.0	41.0	29.5	41.0
44-45	36.29738436622613	39.0	35.0	40.5	28.5	41.0
46-47	36.24575008557859	39.0	35.0	40.5	28.0	41.0
48-49	36.203601048101675	39.0	35.0	41.0	28.0	41.0
50-51	35.23324836896465	38.0	34.0	40.0	26.5	40.5
52-53	35.44417406437894	38.5	34.5	39.5	27.5	40.5
54-55	36.02597977173298	39.0	35.0	40.5	28.0	41.0
56-57	35.9830293116071	39.0	35.0	41.0	28.0	41.0
58-59	35.81783687024562	39.0	35.0	41.0	27.5	41.0
60-61	35.625351229755644	38.0	34.5	40.0	27.0	41.0
62-63	35.36721823833331	38.0	34.0	40.0	26.5	41.0
64-65	35.07848386456232	37.5	34.0	40.0	26.0	41.0
66-67	34.772431262560616	37.0	34.0	40.0	26.0	41.0
68-69	34.484590994840346	36.5	34.0	39.0	26.0	41.0
70-71	34.131513346684876	36.0	33.0	39.0	26.0	41.0
72-73	33.77877892964331	35.5	33.0	39.0	25.5	40.0
74-75	33.413057652009485	35.0	33.0	37.5	25.0	40.0
76-77	33.069571865022894	35.0	32.5	37.0	24.5	39.0
78-79	32.75057787321754	35.0	32.0	36.5	24.0	39.0
80-81	32.41065750943656	35.0	32.0	36.0	24.0	38.5
82-83	32.11305431504032	35.0	32.0	36.0	23.0	37.0
84-85	31.851747719264083	35.0	31.5	35.0	23.0	37.0
86-87	31.563749953933325	35.0	31.0	35.0	21.5	36.5
88-89	31.339789176888885	34.0	31.0	35.0	20.5	36.0
90-91	31.1083591810931	34.0	31.0	35.0	20.0	36.0
92-93	30.961942691771675	34.0	31.0	35.0	20.0	36.0
94-95	30.805628605233068	34.0	30.0	35.0	20.0	35.0
96-97	30.707799578698477	34.0	30.0	35.0	19.0	35.0
98-99	30.533226128615226	34.0	30.0	35.0	19.0	35.0
100	29.143583365832114	33.0	27.0	35.0	15.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.39095675433720345
1101	2	0.42148317483458086
1101	3	0.4077135953409119
1101	4	0.40926303533699837
1101	5	0.3859554135533969
1101	6	0.36311469038433586
1101	7	0.35580332598891573
1101	8	0.45384609252825214
1101	9	0.43220703372127645
1101	10-11	0.3756203206614117
1101	12-13	0.4109002886959061
1101	14-15	0.4665336174120682
1101	16-17	0.43669216214810547
1101	18-19	0.42048852261144987
1101	20-21	0.46750867338046476
1101	22-23	0.504512409442647
1101	24-25	0.36876929830186356
1101	26-27	0.3887547337074153
1101	28-29	0.4533683965358719
1101	30-31	0.3956989835707745
1101	32-33	0.33643061816763975
1101	34-35	0.3443030196095904
1101	36-37	0.3624691309324177
1101	38-39	0.38321560420076395
1101	40-41	0.26381786579155886
1101	42-43	0.1785045562641372
1101	44-45	0.14190042567997097
1101	46-47	0.1520817901644449
1101	48-49	0.308649580128872
1101	50-51	0.29722097796879865
1101	52-53	0.47474806585782403
1101	54-55	0.41504452535287584
1101	56-57	0.35533688971376165
1101	58-59	0.4062560957969126
1101	60-61	0.3905766101338273
1101	62-63	0.39426713829166005
1101	64-65	0.30976804756576115
1101	66-67	0.3154940899515495
1101	68-69	0.16490786238074406
1101	70-71	0.1538238090415618
1101	72-73	0.16459852377442985
1101	74-75	0.04693553331713218
1101	76-77	0.10021984164090014
1101	78-79	-0.01567812255622414
1101	80-81	0.019221881085186965
1101	82-83	0.05276419330013482
1101	84-85	-0.06173155010322873
1101	86-87	-0.14342110040164258
1101	88-89	-0.11465847474962132
1101	90-91	-0.244625981311561
1101	92-93	-0.1760663128329547
1101	94-95	-0.16943826007868878
1101	96-97	-0.2721376664105932
1101	98-99	-0.3632909571820626
1101	100	-0.2608749543898803
1102	1	0.41242041272143837
1102	2	0.4371785792663623
1102	3	0.4153525612028588
1102	4	0.35590494327558275
1102	5	0.33736753979994205
1102	6	0.4053271989482852
1102	7	0.3741604777866314
1102	8	0.3515315400623322
1102	9	0.44132048289342407
1102	10-11	0.4071380746937834
1102	12-13	0.4155215658435978
1102	14-15	0.47034228690038304
1102	16-17	0.42449737395951814
1102	18-19	0.4493718431317859
1102	20-21	0.4609857477610362
1102	22-23	0.5467609126899333
1102	24-25	0.5079567196241612
1102	26-27	0.4414956939837751
1102	28-29	0.4438147677933202
1102	30-31	0.47185845666875537
1102	32-33	0.3780345390700006
1102	34-35	0.45635639640303083
1102	36-37	0.44931064490625516
1102	38-39	0.4007764847031794
1102	40-41	0.5128885385703299
1102	42-43	0.39815191991947785
1102	44-45	0.36175693580717194
1102	46-47	0.3536141903282015
1102	48-49	0.4124516986668425
1102	50-51	0.48880148387167566
1102	52-53	0.57394466944708
1102	54-55	0.6259816404401235
1102	56-57	0.6219112315442104
1102	58-59	0.5900489407483391
1102	60-61	0.5803643812156238
1102	62-63	0.5538864569895452
1102	64-65	0.5579401246265903
1102	66-67	0.4023893977267363
1102	68-69	0.39996811389895726
1102	70-71	0.49022139770126216
1102	72-73	0.3933493433951014
1102	74-75	0.43255014397058034
1102	76-77	0.34798903252142566
1102	78-79	0.32060645127356224
1102	80-81	0.4110680695831519
1102	82-83	0.3639118875378671
1102	84-85	0.2597465088884654
1102	86-87	0.2087389879825956
1102	88-89	0.14959044375573782
1102	90-91	0.10216075567155869
1102	92-93	0.17078849005534735
1102	94-95	0.13098202056992747
1102	96-97	-0.07732368141117263
1102	98-99	-0.0811279919572705
1102	100	0.031070471709792713
1103	1	0.33402629452161037
1103	2	0.4241014502127669
1103	3	0.38866891689921346
1103	4	0.33519179076512984
1103	5	0.32494223909071707
1103	6	0.3115929174623915
1103	7	0.31734408266157743
1103	8	0.3609732950855218
1103	9	0.42875778897833783
1103	10-11	0.36752333053256336
1103	12-13	0.32437863464711825
1103	14-15	0.4621818564351372
1103	16-17	0.4946653472065208
1103	18-19	0.4191295501727694
1103	20-21	0.4410472435674322
1103	22-23	0.48143457238560927
1103	24-25	0.4570556404114612
1103	26-27	0.48655841205264494
1103	28-29	0.47592825042529086
1103	30-31	0.45223082113323443
1103	32-33	0.45012759596600915
1103	34-35	0.4894302416213705
1103	36-37	0.5891221708981291
1103	38-39	0.5135144379421988
1103	40-41	0.3867595380355979
1103	42-43	0.2942088320661398
1103	44-45	0.3073167873480287
1103	46-47	0.33152236945829117
1103	48-49	0.45014686462277353
1103	50-51	0.46888817248059667
1103	52-53	0.5059580393631862
1103	54-55	0.5737885631253832
1103	56-57	0.50256634102508
1103	58-59	0.5203440589796244
1103	60-61	0.4974225640942649
1103	62-63	0.4326828180207798
1103	64-65	0.3997342499619023
1103	66-67	0.4412402210901334
1103	68-69	0.43416845656425807
1103	70-71	0.49144195997222795
1103	72-73	0.31022151514206087
1103	74-75	0.3980708723170423
1103	76-77	0.3269764810273159
1103	78-79	0.40771248887239153
1103	80-81	0.37155471734383383
1103	82-83	0.4218677745763131
1103	84-85	0.2630522714633905
1103	86-87	0.2952669674842099
1103	88-89	0.3493868961665463
1103	90-91	0.19266718740619027
1103	92-93	0.21592370996031107
1103	94-95	0.20356774721050996
1103	96-97	0.16032720350524343
1103	98-99	0.20560428808392217
1103	100	0.20323495364187139
1104	1	0.4476308409969114
1104	2	0.519652241416054
1104	3	0.45625274185499975
1104	4	0.4392063377387103
1104	5	0.33560360353840935
1104	6	0.3844052436198453
1104	7	0.4032909771980684
1104	8	0.43153242390528135
1104	9	0.45708275056659886
1104	10-11	0.46118900815246633
1104	12-13	0.4824224107170991
1104	14-15	0.5334063427485489
1104	16-17	0.5535530279430461
1104	18-19	0.5042218509658483
1104	20-21	0.4738346012426362
1104	22-23	0.5314388657632279
1104	24-25	0.5791344269623337
1104	26-27	0.5798798549824653
1104	28-29	0.6050528041085528
1104	30-31	0.5156335106032586
1104	32-33	0.5319300500852222
1104	34-35	0.5041754067935429
1104	36-37	0.5453221562910855
1104	38-39	0.5177305074607901
1104	40-41	0.4248850862262046
1104	42-43	0.2808514039426697
1104	44-45	0.38583755835401234
1104	46-47	0.41859452482258064
1104	48-49	0.3923275455156059
1104	50-51	0.45891499073284336
1104	52-53	0.6102370420163439
1104	54-55	0.5882563174022124
1104	56-57	0.4924396624953147
1104	58-59	0.5171555469387172
1104	60-61	0.5969448865863072
1104	62-63	0.5932254763791889
1104	64-65	0.5269958053932271
1104	66-67	0.5130846875133912
1104	68-69	0.340349759962713
1104	70-71	0.37530946729452097
1104	72-73	0.46894202035452537
1104	74-75	0.48450939613231725
1104	76-77	0.5028564493832803
1104	78-79	0.3891907759171076
1104	80-81	0.341704985560483
1104	82-83	0.4915050626633075
1104	84-85	0.3128576360694417
1104	86-87	0.2951954339429683
1104	88-89	0.4324755615813771
1104	90-91	0.3133135011422006
1104	92-93	0.4039361628031237
1104	94-95	0.370259532525246
1104	96-97	0.3859798958762042
1104	98-99	0.39608608150078695
1104	100	0.42374826140606103
1105	1	0.425256096399945
1105	2	0.423396234048937
1105	3	0.3767165578743743
1105	4	0.4008853994177741
1105	5	0.32163156734415566
1105	6	0.3426173196097011
1105	7	0.4232275838013422
1105	8	0.3836467293529182
1105	9	0.5458844838127774
1105	10-11	0.41551542247888307
1105	12-13	0.48221114050583225
1105	14-15	0.5660622465155569
1105	16-17	0.5157906668473444
1105	18-19	0.4981808549248541
1105	20-21	0.4949364212333549
1105	22-23	0.6169843490864935
1105	24-25	0.6198404698905975
1105	26-27	0.5878381518296436
1105	28-29	0.5831600466602396
1105	30-31	0.6009012058709615
1105	32-33	0.6357794517124091
1105	34-35	0.6004378275004072
1105	36-37	0.5854662508796267
1105	38-39	0.6315536801728499
1105	40-41	0.6551900487533899
1105	42-43	0.6660575147043417
1105	44-45	0.6839806498304384
1105	46-47	0.5506396402010338
1105	48-49	0.6172091659527794
1105	50-51	0.6438870968160657
1105	52-53	0.6571822367393167
1105	54-55	0.6684945565293461
1105	56-57	0.7107764286098543
1105	58-59	0.7851101437822052
1105	60-61	0.7101599842458484
1105	62-63	0.7138352781000918
1105	64-65	0.6655422794952628
1105	66-67	0.6835433235275872
1105	68-69	0.6556926475278217
1105	70-71	0.6022898531637892
1105	72-73	0.5458003472128539
1105	74-75	0.5823354384028008
1105	76-77	0.5957934300980341
1105	78-79	0.47348167020800247
1105	80-81	0.5198928059705281
1105	82-83	0.5540234085149933
1105	84-85	0.43366769021282536
1105	86-87	0.4529788028374462
1105	88-89	0.47578019933045823
1105	90-91	0.37565700126347323
1105	92-93	0.4236180447072293
1105	94-95	0.47137056141405154
1105	96-97	0.40176575610651
1105	98-99	0.4296571606274249
1105	100	0.5218647950781481
1106	1	0.40826518477617313
1106	2	0.35534508861879743
1106	3	0.3513850131157632
1106	4	0.33393188317219114
1106	5	0.33717381238919586
1106	6	0.3054944709952494
1106	7	0.3482446148440559
1106	8	0.4365774447795161
1106	9	0.38320488484736615
1106	10-11	0.4730850068841548
1106	12-13	0.4128999775166946
1106	14-15	0.5353787109752517
1106	16-17	0.5075353122798489
1106	18-19	0.5228167118787894
1106	20-21	0.4546323887495376
1106	22-23	0.5690207332927741
1106	24-25	0.6064210334385649
1106	26-27	0.5701837340270544
1106	28-29	0.5965704919936314
1106	30-31	0.6174135323108416
1106	32-33	0.5995232592165038
1106	34-35	0.6500219279570771
1106	36-37	0.6602778129318878
1106	38-39	0.6579327999006708
1106	40-41	0.5507427728375589
1106	42-43	0.4448720762814986
1106	44-45	0.5290176784367802
1106	46-47	0.6000487701995638
1106	48-49	0.705974066950148
1106	50-51	0.659719661792515
1106	52-53	0.6750296072373274
1106	54-55	0.6698524073274328
1106	56-57	0.6423357045879712
1106	58-59	0.581912041259109
1106	60-61	0.5724498182182671
1106	62-63	0.6095158240934495
1106	64-65	0.7206248875907093
1106	66-67	0.6791662386434254
1106	68-69	0.6514821450459962
1106	70-71	0.5419218379060808
1106	72-73	0.49153680809584444
1106	74-75	0.6004789675769544
1106	76-77	0.5521761429838747
1106	78-79	0.5106320130053632
1106	80-81	0.5442484854134619
1106	82-83	0.48650032683319466
1106	84-85	0.47694520624306236
1106	86-87	0.5126562837365292
1106	88-89	0.5704014983475432
1106	90-91	0.6234723513178793
1106	92-93	0.4916638722196325
1106	94-95	0.5224102362601144
1106	96-97	0.47473145246700454
1106	98-99	0.4971586691068701
1106	100	0.5952258539512272
1107	1	0.30111043074484556
1107	2	0.32524344344331624
1107	3	0.34084739559011723
1107	4	0.3211833404704265
1107	5	0.3036438946394995
1107	6	0.25636269373243437
1107	7	0.23585646435475383
1107	8	0.2865318945022324
1107	9	0.27871768834409494
1107	10-11	0.30635068784244623
1107	12-13	0.3571362154309057
1107	14-15	0.41816454673106307
1107	16-17	0.402464998804696
1107	18-19	0.37970561946848846
1107	20-21	0.40884515401000954
1107	22-23	0.41586502815584936
1107	24-25	0.4131854896507079
1107	26-27	0.4988183665834498
1107	28-29	0.43039017502243837
1107	30-31	0.4018948662985835
1107	32-33	0.47909682616185023
1107	34-35	0.4017026004632953
1107	36-37	0.5006328452914985
1107	38-39	0.5857815277969323
1107	40-41	0.6372520119474316
1107	42-43	0.5258913915193517
1107	44-45	0.6154485529964475
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2316	70-71	-0.2334561081919233
2316	72-73	-0.3759799050552033
2316	74-75	-0.15316930076047441
2316	76-77	-0.2006767663721618
2316	78-79	-0.40629444798274506
2316	80-81	-0.09078838275944179
2316	82-83	-0.03446818119611095
2316	84-85	-0.044851960867795526
2316	86-87	0.05646068680713512
2316	88-89	-0.05466435611409892
2316	90-91	0.03659342256575471
2316	92-93	0.10898247702288089
2316	94-95	0.11847041278219805
2316	96-97	0.06860704489901792
2316	98-99	0.16002171477246563
2316	100	-0.12574816107139242
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	16.0
1	40.0
2	87.0
3	88.0
4	96.0
5	85.0
6	121.0
7	552.0
8	1352.0
9	2285.0
10	4046.0
11	7188.0
12	10769.0
13	13168.0
14	14367.0
15	15216.0
16	17176.0
17	20261.0
18	23872.0
19	28536.0
20	33434.0
21	40545.0
22	47916.0
23	50639.0
24	51844.0
25	52770.0
26	56711.0
27	64046.0
28	74938.0
29	90266.0
30	109695.0
31	136672.0
32	173408.0
33	228416.0
34	317145.0
35	455715.0
36	707503.0
37	1132510.0
38	1181117.0
39	289111.0
40	329.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.05833097350268	18.422307212854523	12.713426940561284	23.80593487308151
2	15.82608721186963	20.093501854216687	40.36513918462918	23.715271749284504
3	18.128981236331835	26.28501818998131	30.212113742587736	25.373886831099107
4	12.349278320489638	16.5153527349945	38.28357712113545	32.85179182338041
5	12.967855642914653	37.5801224739069	35.284002648282744	14.16801923489571
6	30.714988878635303	36.840054588289476	18.560861853966607	13.88409467910861
7	27.011115104220146	32.48848517741628	22.966790371554723	17.533609346808852
8	25.783811446290244	35.3280585694255	20.81713428534809	18.070995698936166
9	26.69364509492568	15.137336946755806	20.573184640265215	37.5958333180533
10-11	24.61138262304857	25.90655690633101	29.229162594598794	20.25289787602162
12-13	25.17808705467693	23.855349799483026	28.94436077982959	22.02220236601045
14-15	22.65157190261328	25.169027554026556	26.53934776608923	25.640052777270935
16-17	22.12600339702277	27.943061086816247	27.280718659366045	22.650216856794934
18-19	21.99847592689767	27.149544102991474	29.016611589942364	21.83536838016849
20-21	22.750930220190316	26.479412287892373	28.977766407910494	21.791891084006814
22-23	22.54687643448635	26.327798399987966	28.764530128650122	22.360795036875558
24-25	22.200855464935564	26.513994141027936	28.873784538365264	22.411365855671235
26-27	22.122837898931525	26.615000347023543	28.82580160833722	22.436360145707713
28-29	22.09105320918932	26.71060845924287	28.923858597892526	22.274479733675285
30-31	22.512562878574567	26.561079836798797	28.865633518687005	22.06072376593964
32-33	22.249893223149773	26.683869552707236	28.785204955619527	22.281032268523468
34-35	22.43649503171638	26.787441892579224	28.72734971660698	22.04871335909742
36-37	22.41201138551591	26.746270282582106	28.654495879526703	22.18722245237528
38-39	22.294476410257165	26.95378804661173	28.522635414175706	22.2291001289554
40-41	22.387718093628408	26.714562169521766	28.718384493271593	22.179335243578233
42-43	22.21721303996114	26.82684882156491	28.472200123637947	22.483738014836003
44-45	22.206441391357554	26.988256298382606	28.48399721649254	22.3213050937673
46-47	22.217968655440917	26.837513341426828	28.251906311090895	22.692611692041357
48-49	22.232069368653963	26.94115083068565	28.26699483153174	22.559784969128646
50-51	22.18968509348615	27.141368695066664	28.209980951430918	22.45896526001627
52-53	22.275120005714317	27.24142322820621	27.971089880477052	22.51236688560242
54-55	22.185509560345775	27.142883473167583	28.135271563813596	22.536335402673043
56-57	22.276610889476146	27.197292223753173	27.91776250127108	22.6083343854996
58-59	22.239922798467525	27.13237918944076	28.102019873856115	22.5256781382356
60-61	22.437708335778588	27.248623443350272	28.04865300649069	22.26501521438045
62-63	22.26378762887903	27.250670311736574	28.126466658038886	22.359075401345514
64-65	22.220231231100424	27.042227301593126	28.08937451139076	22.6481669559157
66-67	22.160407850760365	27.103370178663937	28.011489141248585	22.72473282932711
68-69	22.088373410491872	27.1423382620201	28.075165751381043	22.694122576106988
70-71	22.146250236039982	26.95577984079602	28.11306588035279	22.78490404281121
72-73	22.075765147247246	27.281722185778378	27.8261849647423	22.81632770223208
74-75	22.12103431464439	27.31742430884464	27.823068336183077	22.738473040327897
76-77	22.08175350343994	27.19921032193227	27.874021957304933	22.845014217322852
78-79	22.03690634540589	27.14832616519366	27.87250891979297	22.942258569607485
80-81	22.070051689260715	27.3222916321092	27.672813242412463	22.93484343621762
82-83	22.252169474408763	27.250806489842073	27.719869399213092	22.777154636536075
84-85	22.152902637030472	27.338967514624063	27.62061545167694	22.88751439666853
86-87	22.026946250265798	27.301570622155847	27.800069925241612	22.871413202336747
88-89	21.979248108636096	27.148277740454745	27.863379301637416	23.009094849271747
90-91	22.28569584094404	27.176828401058106	27.742845738015543	22.794630019982314
92-93	22.162087167413034	27.13826015096557	27.73159724272709	22.968055438894304
94-95	22.054008187960164	27.035031042049923	27.788386094900126	23.122574675089787
96-97	22.084332186207387	27.004693821528424	27.819203977599777	23.09177001466441
98-99	22.069840939173694	26.929899393633093	27.717253968085142	23.283005699108074
100	22.137649187145687	26.992012598769506	27.683470222916124	23.186867991168683
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1523.0
1	1160.5
2	1583.0
3	2371.0
4	1803.5
5	1483.5
6	2243.0
7	4214.0
8	6627.0
9	7756.0
10	6626.0
11	5060.5
12	4926.5
13	5220.0
14	5589.0
15	5924.5
16	6252.5
17	6587.0
18	7078.0
19	7737.0
20	8656.5
21	9906.5
22	11575.0
23	14234.0
24	17747.0
25	21957.5
26	27413.0
27	34109.5
28	41468.5
29	50371.0
30	60844.0
31	71051.0
32	82326.0
33	95198.5
34	108333.0
35	120220.0
36	134781.5
37	148599.5
38	158366.0
39	167818.5
40	172362.0
41	174687.0
42	178966.5
43	184081.0
44	189421.0
45	195671.5
46	199563.5
47	201608.5
48	205674.5
49	209135.0
50	209579.5
51	203544.0
52	194241.0
53	187048.0
54	178799.5
55	166723.5
56	152321.5
57	137188.0
58	122124.5
59	105516.5
60	87761.0
61	72210.0
62	58859.0
63	46081.5
64	34683.0
65	26232.5
66	19681.5
67	15053.5
68	11612.5
69	8765.5
70	6879.0
71	5395.5
72	4211.5
73	3219.0
74	2445.0
75	1820.0
76	1336.0
77	948.5
78	658.5
79	454.5
80	319.0
81	234.0
82	171.0
83	142.0
84	128.5
85	102.5
86	77.0
87	64.5
88	54.0
89	43.5
90	31.0
91	22.5
92	20.5
93	22.0
94	19.5
95	13.0
96	12.0
97	9.0
98	7.0
99	6.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1603395347788277
2	0.09880729021419125
3	0.02931765764566558
4	0.031169492181132886
5	0.026787428280373615
6	0.002456889383689298
7	0.0038686840295406115
8	0.005207138693789259
9	0.0057021835696072515
10-11	0.004198713946752607
12-13	0.007297328169465229
14-15	0.01734490564903042
16-17	0.008287417921101214
18-19	0.016510663358300096
20-21	0.019086730212093728
22-23	0.044086496440902366
24-25	0.01606145597098377
26-27	0.009726715060053527
28-29	0.026750758289572286
30-31	0.014347133901021462
32-33	0.01998514498672638
34-35	0.020379347387840707
36-37	0.021231924673971697
38-39	0.018390000386868402
40-41	0.02469723880469765
42-43	0.03376389403032719
44-45	0.035514886091090825
46-47	0.020370179890140377
48-49	0.012733654305762817
50-51	0.01142270213461517
52-53	0.019829297525820716
54-55	0.02466973631159665
56-57	0.018619187829376733
58-59	0.024009676477172656
60-61	0.024110518951876322
62-63	0.048945270222078965
64-65	0.03141701461904188
66-67	0.01972845505111705
68-69	0.025329796146020635
70-71	0.03876934777470911
72-73	0.036294123395619146
74-75	0.038375145373594786
76-77	0.02192865449919702
78-79	0.015942278500879436
80-81	0.02158945708428469
82-83	0.01731740315592942
84-85	0.01772077305474408
86-87	0.019884302512022715
88-89	0.016675678316906094
90-91	0.02712662569528594
92-93	0.025980688482744294
94-95	0.019150902695996057
96-97	0.013054516725274481
98-99	0.01681319078241109
100	0.03832014038739279
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5454051.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.208327127876494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.22532622153744	48.18075355240041
2	15.03449264351852	19.006102584263427
3	4.344780452527005	8.238789124263905
4	1.7038089204697782	4.307796464337914
5	0.8407046165702033	2.656976621104269
6	0.4880115579278057	1.8507836517411236
7	0.2967077943522212	1.3128082328764132
8	0.2184482440618739	1.1046198456938505
9	0.14067605383919005	0.8002708227113661
>10	0.6534202793206488	7.050217119554536
>50	0.03287061319992284	1.4148148336211483
>100	0.01912971464289012	2.1865671677397076
>500	0.001283213908771788	0.5409546370896278
>1k	2.264494157750544E-4	0.3239619620467595
>5k	3.7741569295836584E-5	0.23223635497238354
>10k+	7.548313859167317E-5	0.7923470255832303
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17735	0.32517114343081865	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15479	0.2838073938069153	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9735	0.17849118022548743	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	1.8334995400666404E-5	1.8334995400666404E-5
4	0.0	0.0	0.0	1.8334995400666404E-5	1.8334995400666404E-5
5	0.0	0.0	0.0	1.8334995400666404E-5	1.8334995400666404E-5
6	0.0	0.0	0.0	1.8334995400666404E-5	1.8334995400666404E-5
7	0.0	0.0	0.0	1.8334995400666404E-5	1.8334995400666404E-5
8	0.0	0.0	0.0	1.8334995400666404E-5	1.8334995400666404E-5
9	0.0	0.0	0.0	1.8334995400666404E-5	1.8334995400666404E-5
10-11	0.0	0.0	0.0	1.8334995400666404E-5	1.8334995400666404E-5
12-13	0.0	0.0	0.0	1.8334995400666404E-5	1.4667996320533123E-4
14-15	0.0	0.0	0.0	1.8334995400666404E-5	1.8334995400666405E-4
16-17	0.0	9.167497700333202E-6	0.0	3.666999080133281E-5	2.0168494940733044E-4
18-19	0.0	1.8334995400666404E-5	0.0	6.417248390233241E-5	3.025274241109957E-4
20-21	0.0	1.8334995400666404E-5	0.0	9.167497700333201E-5	3.8503490341399446E-4
22-23	0.0	1.8334995400666404E-5	0.0	1.4667996320533123E-4	4.308723919156605E-4
24-25	0.0	1.8334995400666404E-5	0.0	2.0168494940733044E-4	4.858773781176597E-4
26-27	0.0	1.8334995400666404E-5	0.0	3.025274241109957E-4	5.04212373518326E-4
28-29	0.0	1.8334995400666404E-5	0.0	9.259172677336533E-4	5.133798712186593E-4
30-31	0.0	1.8334995400666404E-5	0.0	0.003630329089331948	5.225473689189925E-4
32-33	0.0	5.500498620199921E-5	0.0	0.00979088754395586	5.317148666193258E-4
34-35	0.0	5.500498620199921E-5	0.0	0.01886671026728573	5.317148666193258E-4
36-37	0.0	6.417248390233241E-5	0.0	0.03567990104969682	5.40882364319659E-4
38-39	0.0	7.333998160266562E-5	0.0	0.06386078898052108	5.500498620199921E-4
40-41	0.0	7.333998160266562E-5	0.0	0.09693712068332327	5.958873505216582E-4
42-43	0.0	7.333998160266562E-5	0.0	0.13129690206417213	6.233898436226578E-4
44-45	0.0	9.167497700333201E-5	0.0	0.17420995879943185	6.233898436226578E-4
46-47	0.0	9.167497700333201E-5	0.0	0.22568545838680276	6.600598344239906E-4
48-49	0.0	9.167497700333201E-5	0.0	0.2827256290782759	7.700698068279889E-4
50-51	0.0	9.167497700333201E-5	0.0	0.3452204609014474	7.700698068279889E-4
52-53	0.0	9.167497700333201E-5	0.0	0.40901707739806614	8.434097884306546E-4
54-55	0.0	9.167497700333201E-5	0.0	0.48318213379376174	9.167497700333202E-4
56-57	0.0	9.167497700333201E-5	0.0	0.5735003211374444	9.350847654339867E-4
58-59	0.0	9.167497700333201E-5	0.0	0.6768455227133006	9.625872585349862E-4
60-61	0.0	9.167497700333201E-5	0.0	0.7883222947493523	9.717547562353194E-4
62-63	0.0	9.167497700333201E-5	0.0	0.9017517437955751	9.900897516359859E-4
64-65	0.0	9.167497700333201E-5	0.0	1.0224143485273607	9.900897516359859E-4
66-67	0.0	9.167497700333201E-5	0.0	1.1626128908585565	9.900897516359859E-4
68-69	0.0	9.167497700333201E-5	0.0	1.3271236370910358	9.900897516359859E-4
70-71	0.0	9.167497700333201E-5	0.0	1.5107761185218105	9.992572493363189E-4
72-73	0.0	9.167497700333201E-5	0.0	1.6946211174042927	0.0010634297332386515
74-75	0.0	9.167497700333201E-5	0.0	1.8831598751093452	0.0010725972309389847
76-77	0.0	9.167497700333201E-5	0.0	2.0909595454827983	0.0010817647286393178
78-79	0.0	9.167497700333201E-5	0.0	2.333045657255497	0.0010817647286393178
80-81	0.0	9.167497700333201E-5	0.0	2.59698708354579	0.0011000997240399842
82-83	0.0	9.167497700333201E-5	0.0	2.8735888241602434	0.0011734397056426499
84-85	9.167497700333202E-6	9.167497700333201E-5	0.0	3.1556543934040953	0.0011734397056426499
86-87	1.8334995400666404E-5	9.167497700333201E-5	0.0	3.445328985739224	0.0011917747010433163
88	1.8334995400666404E-5	9.167497700333201E-5	0.0	3.685114055589139	0.001228444691844649
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9265	0.0	48.87603	1
GTATCAA	15970	0.0	38.200264	1
TACCTGG	5620	0.0	34.637917	2
GTACCTG	6135	0.0	32.762688	1
TCAACGC	19280	0.0	30.51393	4
CAACGCA	19475	0.0	30.206182	5
ATCAACG	19490	0.0	30.11365	3
ACCTGGG	6625	0.0	29.222954	3
TATCAAC	20380	0.0	29.047537	2
AACGCAG	20300	0.0	28.973545	6
GTACATG	20090	0.0	28.14034	1
TACATGG	20665	0.0	27.122429	2
ACATGGG	20800	0.0	25.98049	3
CATGGGG	12395	0.0	25.551373	4
ACGCAGA	22955	0.0	25.459644	7
CGCAGAG	23480	0.0	24.710985	8
ATGGGGG	7850	0.0	22.08644	5
GCAGAGT	25865	0.0	22.017254	9
GAGTACT	14930	0.0	20.17442	12-13
AGAGTAC	23505	0.0	19.729856	10-11
>>END_MODULE