FastQCFastQC Report
Mon 6 Jun 2016
ERR1211469_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211469_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5454051
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT199460.3657098182616921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181040.3319367567336646No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123970.2272989379820614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104650.045.8800051
GTATCAA174550.036.101221
TACCTGG51000.035.40612
GTACCTG58450.031.2164961
TCAACGC214000.028.9853444
ATCAACG216050.028.6668153
ACCTGGG62550.028.3976763
CAACGCA218700.028.3839175
TATCAAC223100.027.8854262
AACGCAG226050.027.5857476
GTACATG209900.025.4956911
TACATGG216100.024.327872
ACGCAGA258200.024.0052837
CATGGGG125200.023.5707634
CGCAGAG262400.023.567338
ACATGGG214250.023.5340793
ATGGGGG73700.022.9537335
GCAGAGT292950.020.5640589
GAGTACT168700.019.9722212-13
TGGGGGG67250.019.8446926
Produced by FastQC (version 0.11.5)