##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211469_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5454051 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.050533080823776 33.0 31.0 34.0 30.0 34.0 2 32.28798428910914 34.0 31.0 34.0 30.0 34.0 3 32.29943596053649 34.0 31.0 34.0 30.0 34.0 4 35.9555132506095 37.0 35.0 37.0 35.0 37.0 5 35.87535246736783 37.0 35.0 37.0 35.0 37.0 6 35.89269407271769 37.0 35.0 37.0 35.0 37.0 7 35.81644157709563 37.0 35.0 37.0 35.0 37.0 8 35.80947794584246 37.0 35.0 37.0 35.0 37.0 9 37.44969454814412 39.0 37.0 39.0 34.0 39.0 10-11 37.40722611504733 39.0 37.0 39.0 34.0 39.0 12-13 37.319988298605935 39.0 37.0 39.0 34.0 39.0 14-15 38.531776747228804 40.0 38.0 41.0 34.0 41.0 16-17 38.45361878720973 40.0 38.0 41.0 34.0 41.0 18-19 38.43747234853507 40.0 38.0 41.0 34.0 41.0 20-21 38.373842580496586 40.0 38.0 41.0 34.0 41.0 22-23 38.263714439047234 40.0 38.0 41.0 34.0 41.0 24-25 38.14662523324406 40.0 37.5 41.0 33.0 41.0 26-27 37.960233870200334 40.0 37.0 41.0 33.0 41.0 28-29 37.99447869116002 40.0 37.0 41.0 33.0 41.0 30-31 37.91080785639885 40.0 37.0 41.0 33.0 41.0 32-33 37.720859779272324 40.0 37.0 41.0 33.0 41.0 34-35 37.60084660007763 40.0 37.0 41.0 32.0 41.0 36-37 37.53751926778829 40.0 37.0 41.0 32.0 41.0 38-39 37.43738186533276 40.0 36.5 41.0 32.0 41.0 40-41 37.26764115333722 40.0 36.0 41.0 31.0 41.0 42-43 37.10846451564168 39.0 36.0 41.0 31.0 41.0 44-45 36.916440550335885 39.0 36.0 41.0 30.5 41.0 46-47 36.64620554519934 39.0 35.0 41.0 30.0 41.0 48-49 36.40808327608231 39.0 35.0 40.0 29.5 41.0 50-51 36.20216605968665 39.0 35.0 40.0 28.5 41.0 52-53 35.993545531569104 39.0 35.0 40.0 28.0 41.0 54-55 35.78545396806887 38.0 34.5 40.0 28.0 41.0 56-57 35.58081277567811 38.0 34.0 40.0 27.0 41.0 58-59 35.310575845367055 38.0 34.0 40.0 26.5 41.0 60-61 34.98164593620412 37.5 34.0 40.0 26.0 41.0 62-63 34.65093606568769 37.0 33.0 40.0 26.0 41.0 64-65 34.32834328098509 36.5 33.0 39.0 26.0 41.0 66-67 33.975926517738834 36.0 32.5 39.0 25.0 40.0 68-69 33.57066087207472 35.5 32.0 39.0 24.5 40.0 70-71 33.17439651737763 35.0 31.5 38.0 23.5 40.0 72-73 32.765829930816565 35.0 31.0 37.5 23.0 39.5 74-75 32.357145175210135 35.0 31.0 37.0 22.0 39.0 76-77 30.843310962805447 33.5 29.5 35.5 20.5 37.5 78-79 31.685836271057973 34.0 30.5 36.0 22.0 38.0 80-81 31.664483885464218 34.0 31.0 36.0 22.0 37.5 82-83 31.387721346940097 34.0 30.0 35.0 20.5 37.0 84-85 31.087460403285558 34.0 30.0 35.0 20.0 36.5 86-87 30.791062459811982 34.0 30.0 35.0 20.0 36.0 88-89 30.511137776306086 34.0 29.0 35.0 19.5 36.0 90-91 30.22041753918326 34.0 29.0 35.0 18.5 35.0 92-93 30.003725304365506 34.0 29.0 35.0 18.0 35.0 94-95 29.779454665898797 34.0 29.0 35.0 18.0 35.0 96-97 29.565396344845325 33.0 29.0 35.0 17.5 35.0 98-99 29.3936966302662 33.0 28.0 35.0 17.0 35.0 100 27.445954209082387 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2614537217276691 1101 2 0.2108472070941616 1101 3 0.2500726548523815 1101 4 0.16038376280629763 1101 5 0.1517736944556134 1101 6 0.18949588884412094 1101 7 0.17480470303958384 1101 8 0.17923817902447325 1101 9 0.22125493434062804 1101 10-11 0.1903309299907079 1101 12-13 0.19376643573953345 1101 14-15 0.2595405746202104 1101 16-17 0.3004344795444496 1101 18-19 0.30560892308829324 1101 20-21 0.2882164347685148 1101 22-23 0.2292038693254952 1101 24-25 0.2160709781789123 1101 26-27 0.2016959000330374 1101 28-29 0.2656724780005746 1101 30-31 0.2638723114070629 1101 32-33 0.2803460576709398 1101 34-35 0.2552922062454215 1101 36-37 0.3069114707589051 1101 38-39 0.3086335606539947 1101 40-41 0.39002345847931963 1101 42-43 0.37317018400306523 1101 44-45 0.34092021860536903 1101 46-47 0.3640699573512478 1101 48-49 0.34016391110969835 1101 50-51 0.3234124667740019 1101 52-53 0.31128820911788324 1101 54-55 0.22600144077177475 1101 56-57 0.22777557334369192 1101 58-59 0.249035682287591 1101 60-61 0.23670316605636543 1101 62-63 0.3326518703929935 1101 64-65 0.20399247312352742 1101 66-67 0.12459822824614264 1101 68-69 0.06895594982950826 1101 70-71 0.0015867949817689464 1101 72-73 -0.035115433928289974 1101 74-75 0.03970092795444913 1101 76-77 0.2346853105901019 1101 78-79 0.36972102124026307 1101 80-81 0.2973091575886251 1101 82-83 0.23566969832747375 1101 84-85 0.08299503504170858 1101 86-87 0.14588747881613173 1101 88-89 0.1504557266290938 1101 90-91 0.06173354184636182 1101 92-93 -0.04133313668227956 1101 94-95 0.006275704703192275 1101 96-97 -0.0604705842436033 1101 98-99 -0.1282270466355797 1101 100 -0.14695013393721723 1102 1 0.25197977889821743 1102 2 0.19009657973676752 1102 3 0.27170413349210065 1102 4 0.17389848819981069 1102 5 0.15271833274609037 1102 6 0.14786287691018174 1102 7 0.19861844650688454 1102 8 0.13338371390929638 1102 9 0.18853651906691482 1102 10-11 0.1944084317797774 1102 12-13 0.21260199009839198 1102 14-15 0.2919727002401018 1102 16-17 0.324407813567511 1102 18-19 0.2963924112812464 1102 20-21 0.30682146184093995 1102 22-23 0.31002524858707403 1102 24-25 0.26784658920950477 1102 26-27 0.28096662341489065 1102 28-29 0.2895431321654627 1102 30-31 0.3690205190030653 1102 32-33 0.40600143295262825 1102 34-35 0.43663488366913583 1102 36-37 0.47679502953042174 1102 38-39 0.48260569439859324 1102 40-41 0.49531009454608466 1102 42-43 0.3802488604245582 1102 44-45 0.4646333163994072 1102 46-47 0.51073492211966 1102 48-49 0.4997778025803541 1102 50-51 0.4725368950764448 1102 52-53 0.442692166362157 1102 54-55 0.4870337496979644 1102 56-57 0.5604501251381109 1102 58-59 0.5435173733383252 1102 60-61 0.4478430024346167 1102 62-63 0.4856859392172339 1102 64-65 0.5228570009468356 1102 66-67 0.44406289530174803 1102 68-69 0.5470638139063624 1102 70-71 0.4449173971119862 1102 72-73 0.49218107198181116 1102 74-75 0.3256733555622091 1102 76-77 0.4419667575167665 1102 78-79 0.39461374396013227 1102 80-81 0.44002572569119636 1102 82-83 0.381942043534341 1102 84-85 0.36321922245182137 1102 86-87 0.33781990691166186 1102 88-89 0.24082549879436854 1102 90-91 0.2968016129463713 1102 92-93 0.23894843989632975 1102 94-95 0.35628078743135916 1102 96-97 0.35536029854398166 1102 98-99 0.4016787798988659 1102 100 0.5576053411562363 1103 1 0.25132090682438246 1103 2 0.16214703421384513 1103 3 0.24243935365614533 1103 4 0.15186238296012533 1103 5 0.11666377552599272 1103 6 0.12896336329454527 1103 7 0.09484844278747318 1103 8 0.18890336113773287 1103 9 0.17663145580208095 1103 10-11 0.20814883186469046 1103 12-13 0.20584229024871092 1103 14-15 0.2787363181305693 1103 16-17 0.25043216479279806 1103 18-19 0.23542184851101666 1103 20-21 0.18908533484395917 1103 22-23 0.23084448614033448 1103 24-25 0.20434186259715403 1103 26-27 0.3490800676167112 1103 28-29 0.3720721489574501 1103 30-31 0.3971773957583693 1103 32-33 0.4066540979085431 1103 34-35 0.3755630369268701 1103 36-37 0.38621844386951665 1103 38-39 0.3953134567557939 1103 40-41 0.4353604407494345 1103 42-43 0.5140396844367316 1103 44-45 0.4825085880890043 1103 46-47 0.5443919260669006 1103 48-49 0.42699336720382064 1103 50-51 0.4071589412372205 1103 52-53 0.3887046112557755 1103 54-55 0.45425186841551835 1103 56-57 0.4887055979135084 1103 58-59 0.5985055428414299 1103 60-61 0.40667599301809787 1103 62-63 0.5543667409847188 1103 64-65 0.628274535687936 1103 66-67 0.5464711652986338 1103 68-69 0.465336837712492 1103 70-71 0.5092139225778709 1103 72-73 0.5069986364199224 1103 74-75 0.3928332245261785 1103 76-77 0.426057131642434 1103 78-79 0.5742651031003625 1103 80-81 0.610172718879614 1103 82-83 0.6254849136959528 1103 84-85 0.4515973144129184 1103 86-87 0.5365404363928548 1103 88-89 0.4950344375083624 1103 90-91 0.4642034947394258 1103 92-93 0.4027445259252609 1103 94-95 0.47379146111037684 1103 96-97 0.47437410679056313 1103 98-99 0.4305091424425882 1103 100 0.44500196093253663 1104 1 0.25696020713351686 1104 2 0.24716238184433337 1104 3 0.290598928058877 1104 4 0.06551713942868531 1104 5 0.14952396362917142 1104 6 0.16538725959275524 1104 7 0.21340556757596119 1104 8 0.17216347497708995 1104 9 0.28302905260272837 1104 10-11 0.25526200980370106 1104 12-13 0.2845311514251776 1104 14-15 0.3472110633093948 1104 16-17 0.2906496731730783 1104 18-19 0.35076756779509566 1104 20-21 0.2198002402360686 1104 22-23 0.33799677545255946 1104 24-25 0.32231633031047835 1104 26-27 0.3513335789375063 1104 28-29 0.42731428112112013 1104 30-31 0.3649999843852143 1104 32-33 0.3101917591576395 1104 34-35 0.4346274218919959 1104 36-37 0.30725667677262436 1104 38-39 0.41068176917170973 1104 40-41 0.4718610581357723 1104 42-43 0.522777602059854 1104 44-45 0.5648579598156473 1104 46-47 0.5130685487281568 1104 48-49 0.5923098519309278 1104 50-51 0.48735787000878616 1104 52-53 0.4669644623049223 1104 54-55 0.5748251333005356 1104 56-57 0.6295509086782829 1104 58-59 0.5204723602882453 1104 60-61 0.46276205715119545 1104 62-63 0.5996375206934914 1104 64-65 0.648193323276324 1104 66-67 0.6507278478487564 1104 68-69 0.6815754839811845 1104 70-71 0.5793519318021438 1104 72-73 0.6506022204687625 1104 74-75 0.7220503895727504 1104 76-77 0.5925639809107501 1104 78-79 0.6712623052378994 1104 80-81 0.7667056901767815 1104 82-83 0.5829806527405061 1104 84-85 0.6203161063697671 1104 86-87 0.7170399289006006 1104 88-89 0.6892668819282335 1104 90-91 0.6269692217092739 1104 92-93 0.6405634218378147 1104 94-95 0.7036375479594703 1104 96-97 0.5501672792962928 1104 98-99 0.614786438665849 1104 100 0.7220611952096618 1105 1 0.22249483711259188 1105 2 0.11274075408937279 1105 3 0.19901865647860717 1105 4 0.139087437443429 1105 5 0.07648515059035788 1105 6 0.11886719529491074 1105 7 0.18923451229379395 1105 8 0.12927089430672822 1105 9 0.14288475245842847 1105 10-11 0.19643449986507733 1105 12-13 0.19834688301868653 1105 14-15 0.2969694119566313 1105 16-17 0.29157257742932075 1105 18-19 0.32814368961566487 1105 20-21 0.20256410188882512 1105 22-23 0.34111337079136916 1105 24-25 0.37348980703951185 1105 26-27 0.38328002755061874 1105 28-29 0.4168110806179186 1105 30-31 0.4730163712904911 1105 32-33 0.46538775657585774 1105 34-35 0.4018437446638714 1105 36-37 0.4877163405656262 1105 38-39 0.5085450170349546 1105 40-41 0.4469537432284554 1105 42-43 0.4359025840737232 1105 44-45 0.4487279992412354 1105 46-47 0.5427926517855894 1105 48-49 0.5582123445000846 1105 50-51 0.5851302622256256 1105 52-53 0.4559349979421228 1105 54-55 0.4580828221137807 1105 56-57 0.5507572743290936 1105 58-59 0.5456460343508098 1105 60-61 0.6658565758012642 1105 62-63 0.5803807281033642 1105 64-65 0.5779076829906842 1105 66-67 0.5722904005224194 1105 68-69 0.5804592339760362 1105 70-71 0.6593064628677894 1105 72-73 0.562910077221062 1105 74-75 0.6528653359432539 1105 76-77 0.597841255076915 1105 78-79 0.7439489190356205 1105 80-81 0.7365515233559456 1105 82-83 0.6990541265917578 1105 84-85 0.6379970262729096 1105 86-87 0.7558599154983661 1105 88-89 0.760730233391584 1105 90-91 0.8121220935288136 1105 92-93 0.767192595133654 1105 94-95 0.8632091286421613 1105 96-97 0.8080035171325299 1105 98-99 0.8629946090962441 1105 100 1.0339850738002063 1106 1 0.2566810438374887 1106 2 0.1334862801032557 1106 3 0.1784124379797305 1106 4 0.12017840223541754 1106 5 0.14952713439954834 1106 6 0.15173104969317563 1106 7 0.1613849060773731 1106 8 0.18549515517372583 1106 9 0.2126847801116014 1106 10-11 0.14876909864354104 1106 12-13 0.2127661976520301 1106 14-15 0.29516515760051476 1106 16-17 0.36172964569307453 1106 18-19 0.2933563663034846 1106 20-21 0.33028656052995586 1106 22-23 0.32023210081560194 1106 24-25 0.36511540238588935 1106 26-27 0.3159866031613774 1106 28-29 0.3396310166910652 1106 30-31 0.392045043200298 1106 32-33 0.49368258763657735 1106 34-35 0.43446779934917146 1106 36-37 0.342050597023686 1106 38-39 0.46762026842169746 1106 40-41 0.48589427805308816 1106 42-43 0.42994363796002233 1106 44-45 0.5017164716403144 1106 46-47 0.5833121695853194 1106 48-49 0.4977256903354572 1106 50-51 0.5332393915991602 1106 52-53 0.5657396099693202 1106 54-55 0.4455627168212075 1106 56-57 0.605960851346957 1106 58-59 0.6746271399535786 1106 60-61 0.6836137884053741 1106 62-63 0.6289841387416786 1106 64-65 0.6556803658012313 1106 66-67 0.6461392824055494 1106 68-69 0.5667839892339543 1106 70-71 0.6872320550014805 1106 72-73 0.6467197205466775 1106 74-75 0.6136505445881966 1106 76-77 0.6110752771189709 1106 78-79 0.6946181049698801 1106 80-81 0.6891522647285022 1106 82-83 0.7069472322787398 1106 84-85 0.7419349608002399 1106 86-87 0.5453271708859369 1106 88-89 0.6946514597889433 1106 90-91 0.7427645769371871 1106 92-93 0.7178017214918739 1106 94-95 0.7818370652977578 1106 96-97 0.8257039321823036 1106 98-99 0.8112814501226318 1106 100 0.8612059815281938 1107 1 0.21640595743880908 1107 2 0.22318000037767405 1107 3 0.21116814221299762 1107 4 0.1253910709168764 1107 5 0.11585647109375685 1107 6 0.1205818328586048 1107 7 0.13355874152367875 1107 8 0.11492046486196017 1107 9 0.15070963198142096 1107 10-11 0.11112892493686388 1107 12-13 0.23031491498672096 1107 14-15 0.24719842822338478 1107 16-17 0.2497816639402899 1107 18-19 0.22009324948975717 1107 20-21 0.1985322846494597 1107 22-23 0.2694469868608351 1107 24-25 0.2663271583674245 1107 26-27 0.26687473473023005 1107 28-29 0.2922205767821353 1107 30-31 0.24648598343934935 1107 32-33 0.2707760789453104 1107 34-35 0.3087202015403321 1107 36-37 0.3826214354707176 1107 38-39 0.3706970159794096 1107 40-41 0.35681055308526766 1107 42-43 0.43306799727687206 1107 44-45 0.39088835460914595 1107 46-47 0.44413878992418176 1107 48-49 0.5174336263628732 1107 50-51 0.4620866391819973 1107 52-53 0.5203539123610383 1107 54-55 0.5067668424959137 1107 56-57 0.5063824667467358 1107 58-59 0.6083543951723769 1107 60-61 0.5669793523957409 1107 62-63 0.5463927035493015 1107 64-65 0.6242983991927176 1107 66-67 0.627248963971553 1107 68-69 0.6204833607926261 1107 70-71 0.7225162090964048 1107 72-73 0.6856511726036629 1107 74-75 0.660890643968564 1107 76-77 0.6252767127010515 1107 78-79 0.7315481122951972 1107 80-81 0.732558346721266 1107 82-83 0.6947964345982136 1107 84-85 0.5852673659960779 1107 86-87 0.5643115267801662 1107 88-89 0.5600133402218646 1107 90-91 0.4057071248496946 1107 92-93 0.5397282355938238 1107 94-95 0.7093061134183891 1107 96-97 0.7456834472652893 1107 98-99 0.8416511726584694 1107 100 0.9506256659124972 1108 1 0.22435679529318975 1108 2 0.14917593558273978 1108 3 0.22535376484944436 1108 4 0.11725861336907428 1108 5 0.13485411594178487 1108 6 0.12189329010924155 1108 7 0.14592662092820774 1108 8 0.12391035854133747 1108 9 0.1732190032483416 1108 10-11 0.14360597014751164 1108 12-13 0.1751898237200109 1108 14-15 0.1905642206883016 1108 16-17 0.24250451987904142 1108 18-19 0.26145541968202934 1108 20-21 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74-75 -0.3603399547010575 2316 76-77 -0.3330282818901864 2316 78-79 -0.24761439569842025 2316 80-81 -0.2640320394227409 2316 82-83 -0.1599711976774678 2316 84-85 -0.08537573917302765 2316 86-87 -0.1574205032698579 2316 88-89 -0.2034186258792232 2316 90-91 -0.14102191693227084 2316 92-93 0.022657455476565502 2316 94-95 0.03449048955766543 2316 96-97 -0.009462350333336644 2316 98-99 -0.07100025782545316 2316 100 -0.17769146104706124 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 4.0 11 27.0 12 67.0 13 176.0 14 592.0 15 1493.0 16 3389.0 17 7017.0 18 12446.0 19 18741.0 20 26646.0 21 35817.0 22 47267.0 23 54044.0 24 58379.0 25 64661.0 26 73709.0 27 88764.0 28 105771.0 29 128210.0 30 154950.0 31 187033.0 32 229780.0 33 289843.0 34 381257.0 35 517228.0 36 752963.0 37 1109444.0 38 977190.0 39 127130.0 40 10.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.98273046928216 20.290057560824096 14.111303817848677 23.615908152045066 2 15.206578181866885 21.415307908279292 39.75302829848915 23.62508561136468 3 17.2882014130479 27.213236607232894 30.8538133114877 24.644748668231507 4 12.142992037051638 17.649014211561767 37.911279957315806 32.29671379407078 5 12.520762356891233 38.31465492820749 35.26244407525439 13.902138639646894 6 30.537363561573056 37.83002509893167 18.08657728789624 13.546034051599031 7 26.77100012449462 33.01538617809038 22.80273873493299 17.410874962482016 8 25.91098142870344 36.09958034531669 20.336713844204116 17.65272438177575 9 26.66853561454985 15.273735164462218 20.41178947970581 37.64593974128212 10-11 24.7262355999238 26.467720965572195 28.905111081652883 19.900932352851118 12-13 25.203999742576666 24.31551336795347 28.826197261448417 21.65428962802145 14-15 22.626548596630283 25.629802508264042 26.43922838271956 25.304420512386116 16-17 22.099675284790457 28.438103340477923 27.162051408046555 22.30016996668506 18-19 22.00829942468751 27.476357915783712 29.09249508502258 21.4228475745062 20-21 22.809996243075407 26.698138167915275 29.077496241700253 21.414369347309066 22-23 22.62179273917614 26.578723464331695 28.839731131935796 21.95975266455637 24-25 22.272757567154013 26.79937664654064 28.940263365828155 21.98760242047719 26-27 22.252981276264933 26.80913597993414 28.945946491041504 21.991936252759423 28-29 22.20539767093897 26.907450778561536 29.047962133483484 21.839189417016005 30-31 22.57210003543292 26.76335163346683 29.007653602629645 21.656894728470604 32-33 22.352580073327665 26.8542134071255 28.945542582926343 21.84766393662049 34-35 22.52968315083246 26.973494398731752 28.873689115837287 21.623133334598503 36-37 22.572305597016054 26.892594985337787 28.76769130249956 21.7674081151466 38-39 22.48587107578395 27.059739861126886 28.686095994451016 21.768293068638148 40-41 22.493464368373548 26.8956127953984 28.767643225794327 21.843279610433726 42-43 22.345543686297578 27.028025857045467 28.605438384884472 22.02099207177248 44-45 22.336301593222945 27.129621573680453 28.541914183370675 21.992162649725927 46-47 22.374301079281107 27.056298627173796 28.332190220670405 22.237210072874696 48-49 22.377308988704296 27.13999873110827 28.32762270934788 22.155069570839547 50-51 22.368377744904652 27.35337167784195 28.158551349537532 22.119699227715863 52-53 22.42760035699601 27.411490155793828 27.956142847909526 22.20476663930064 54-55 22.332530976722488 27.31096489249778 28.121929968349452 22.234574162430278 56-57 22.448358618246612 27.379132149387843 27.866626353088638 22.305882879276908 58-59 22.357199448539692 27.32566870246391 28.066819548567505 22.250312300428888 60-61 22.585348425217965 27.441408837497093 28.000794695745125 21.972448041539813 62-63 22.38249386473649 27.450121586683764 28.074036122275075 22.09334842630468 64-65 22.32860077090552 27.22252333490898 28.063892893198673 22.38498300098683 66-67 22.31853347731429 27.267888010981572 27.95293443713843 22.460644074565707 68-69 22.201477723843915 27.317903534078912 28.009923007481408 22.470695734595765 70-71 22.262824314021458 27.173507657677977 28.036639797042106 22.527028231258463 72-73 22.17122689291968 27.49886154404424 27.715248397016424 22.614663166019653 74-75 22.189122467584568 27.520506486753295 27.680860098968 22.609510946694137 76-77 22.13163455948772 27.392774099315638 27.816654685931308 22.658936655265332 78-79 22.04814517392613 27.39037322058441 27.818169166108248 22.74331243938121 80-81 22.071335614036457 27.547076747050525 27.57670785714908 22.80487978176394 82-83 22.175445101646943 27.52358745091955 27.626582693406736 22.674384754026768 84-85 22.06620061906174 27.605022545928538 27.548403281735073 22.780373553274647 86-87 21.95267567313052 27.54349253139842 27.67306670085055 22.830765094620503 88-89 21.891033467300584 27.385124325835037 27.741005120256478 22.982837086607898 90-91 22.150099050283654 27.389741684342646 27.634845746743043 22.82531351863066 92-93 22.026153290987118 27.38296977730866 27.590882892516017 22.99999403918821 94-95 21.84535753458326 27.320003815071736 27.640011298481674 23.194627351863332 96-97 21.84276450436837 27.279445206534575 27.634379515995334 23.243410773101722 98-99 21.730246380867257 27.216169805736257 27.563082443717356 23.49050136967913 100 20.85725178646845 27.78027891408832 27.787229118532526 23.575240180910704 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1625.0 1 1241.5 2 1644.5 3 2445.0 4 1897.0 5 1520.5 6 2298.5 7 4517.5 8 7020.0 9 8153.0 10 7213.5 11 5804.5 12 5778.0 13 6155.5 14 6589.0 15 7008.5 16 7372.0 17 7776.0 18 8280.5 19 8924.0 20 9706.5 21 10917.5 22 12745.0 23 15449.0 24 18849.0 25 23164.0 26 28748.5 27 35192.0 28 42976.0 29 52071.0 30 62444.0 31 72827.0 32 84276.0 33 97519.5 34 110462.5 35 122098.5 36 136769.5 37 151128.0 38 160730.5 39 169530.0 40 174002.0 41 176395.0 42 180001.5 43 184119.0 44 189017.5 45 195013.0 46 199430.5 47 201513.5 48 205785.0 49 208876.0 50 208428.5 51 202212.5 52 192329.5 53 184282.0 54 175002.5 55 162892.5 56 148593.5 57 133582.0 58 118881.5 59 102351.5 60 84859.5 61 69483.0 62 56464.0 63 44189.0 64 33306.0 65 25125.5 66 18749.0 67 14255.0 68 10889.5 69 8151.0 70 6248.5 71 4871.0 72 3847.0 73 2981.5 74 2283.0 75 1698.0 76 1194.5 77 833.5 78 573.0 79 395.0 80 260.0 81 165.5 82 123.0 83 97.5 84 68.0 85 47.0 86 35.5 87 21.5 88 15.0 89 14.0 90 13.0 91 7.5 92 3.5 93 4.0 94 3.5 95 2.0 96 1.0 97 1.5 98 3.0 99 2.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003080279227311956 2 0.06505256368156441 3 4.583748850166601E-4 4 7.517348114273226E-4 5 0.0012834496780466482 6 6.233898436226578E-4 7 0.0 8 7.884048022286554E-4 9 3.666999080133281E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.517348114273226E-4 18-19 0.0010725972309389847 20-21 0.0022368694388813014 22-23 9.167497700333202E-6 24-25 4.5837488501666014E-5 26-27 2.0168494940733044E-4 28-29 9.259172677336533E-4 30-31 0.0015309721159556448 32-33 0.0032086241951166205 34-35 0.0044279013892609366 36-37 0.001182607203342983 38-39 0.001457632134352979 40-41 0.005381321150095589 42-43 0.006838953284448568 44-45 0.005399656145496257 46-47 0.006866455777549568 48-49 0.00867245282451521 50-51 0.007764870552182223 52-53 0.0038228465410389454 54-55 0.0021451944618779695 56-57 0.0025668993560932964 58-59 0.003080279227311956 60-61 0.007178150699360897 62-63 0.013567896596493139 64-65 0.0037586740571366128 66-67 0.010120917461167856 68-69 0.012174436946042491 70-71 0.005665513578805919 72-73 0.007196485694761563 74-75 0.0030619442319112897 76-77 0.0028694267802042922 78-79 0.007324830662566228 80-81 0.0059313710121155815 82-83 0.016253973422690766 84-85 0.017601595584639747 86-87 0.021809477029092687 88-89 0.020195997433834042 90-91 0.023193769181843002 92-93 0.03251711434308187 94-95 0.03650497584272681 96-97 0.022203679430207016 98-99 0.017399910635232416 100 0.017858285520249078 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5454051.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.14321713133694 #Duplication Level Percentage of deduplicated Percentage of total 1 75.5529716883298 46.95104724545631 2 15.407047070683907 19.148869429324776 3 4.473785997739255 8.340463639699362 4 1.7705734461263691 4.401165204384418 5 0.8742269872129218 2.7163638744223557 6 0.5091443992147776 1.898392257096481 7 0.30472858048201906 1.3255770040112733 8 0.21002759905634316 1.0441432553385368 9 0.15408635135505155 0.8617879430309197 >10 0.6858875536634206 7.22892672886765 >50 0.036234068503251914 1.5471357222845559 >100 0.019342155528860476 2.2111221977985185 >500 0.0013723075429859998 0.5721076565145776 >1k 4.5743564858606004E-4 0.5578705106112116 >5k 0.0 0.0 >10k+ 1.1435891214647138E-4 1.1950273311591575 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19946 0.3657098182616921 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18104 0.3319367567336646 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12397 0.2272989379820614 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 1.8334995400666404E-5 5 0.0 0.0 0.0 0.0 1.8334995400666404E-5 6 0.0 0.0 0.0 0.0 9.167497700333201E-5 7 0.0 0.0 0.0 0.0 9.167497700333201E-5 8 0.0 0.0 0.0 0.0 1.1000997240399842E-4 9 0.0 0.0 0.0 0.0 1.1000997240399842E-4 10-11 0.0 0.0 0.0 0.0 1.1000997240399842E-4 12-13 0.0 0.0 0.0 0.0 1.2834496780466482E-4 14-15 0.0 0.0 0.0 0.0 1.6501495860599764E-4 16-17 0.0 0.0 0.0 0.0 1.8334995400666403E-4 18-19 0.0 0.0 0.0 2.7502493100999606E-5 1.9251745170699723E-4 20-21 0.0 0.0 0.0 3.666999080133281E-5 2.1085244710766364E-4 22-23 0.0 0.0 0.0 9.167497700333203E-5 2.2001994480799685E-4 24-25 0.0 0.0 0.0 1.4667996320533123E-4 2.2001994480799685E-4 26-27 0.0 0.0 0.0 2.3835494020866323E-4 2.3835494020866326E-4 28-29 0.0 0.0 0.0 8.434097884306546E-4 2.3835494020866326E-4 30-31 0.0 0.0 0.0 0.0035111516192276163 2.4752243790899647E-4 32-33 0.0 0.0 0.0 0.009662542576151196 2.9335992641066246E-4 34-35 0.0 0.0 0.0 0.018747532797181398 3.116949218113289E-4 36-37 0.0 0.0 0.0 0.03547821610028949 3.116949218113289E-4 38-39 0.0 0.0 0.0 0.06364076903571309 3.2086241951166205E-4 40-41 0.0 0.0 0.0 0.09664376075691261 3.483649126126617E-4 42-43 0.0 9.167497700333202E-6 0.0 0.13067351222054946 3.575324103129949E-4 44-45 0.0 1.8334995400666404E-5 0.0 0.17321986904779585 3.8503490341399446E-4 46-47 0.0 3.666999080133281E-5 0.0 0.22476870861676945 4.033698988146609E-4 48-49 0.0 6.417248390233241E-5 0.0 0.2814971843864313 4.767098804173265E-4 50-51 0.0 8.250747930299882E-5 0.0 0.34350613883148506 4.767098804173265E-4 52-53 0.0 9.167497700333201E-5 0.0 0.40706440038789515 5.408823643196589E-4 54-55 0.0 9.167497700333201E-5 0.0 0.4806519044284698 6.050548482219913E-4 56-57 0.0 9.167497700333201E-5 0.0 0.5701908544676242 6.050548482219913E-4 58-59 0.0 9.167497700333201E-5 0.0 0.6724084538263393 6.233898436226578E-4 60-61 0.0 9.167497700333201E-5 0.0 0.7825101012073411 6.233898436226578E-4 62-63 0.0 9.167497700333201E-5 0.0 0.8946010955893152 6.783948298246569E-4 64-65 0.0 9.167497700333201E-5 0.0 1.0137877331913472 6.783948298246569E-4 66-67 0.0 9.167497700333201E-5 0.0 1.1519694260284694 6.783948298246569E-4 68-69 0.0 9.167497700333201E-5 0.0 1.313812430430152 6.783948298246569E-4 70-71 0.0 9.167497700333201E-5 0.0 1.4946138200761232 6.875623275249902E-4 72-73 0.0 9.167497700333201E-5 0.0 1.6750210073209804 7.884048022286554E-4 74-75 0.0 9.167497700333201E-5 0.0 1.8614054030664546 7.884048022286554E-4 76-77 0.0 9.167497700333201E-5 0.0 2.0667573515539184 7.975722999289886E-4 78-79 0.0 9.167497700333201E-5 0.0 2.30589152906711 8.067397976293218E-4 80-81 0.0 9.167497700333201E-5 0.0 2.5649558465808258 8.800797792319874E-4 82-83 0.0 9.167497700333201E-5 0.0 2.8366804784187023 9.53419760834653E-4 84-85 0.0 9.167497700333201E-5 0.0 3.1138597713882765 9.53419760834653E-4 86-87 0.0 9.167497700333201E-5 0.0 3.398116372582508 9.717547562353194E-4 88 0.0 9.167497700333201E-5 0.0 3.6322175938582166 9.900897516359859E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10465 0.0 45.880005 1 GTATCAA 17455 0.0 36.10122 1 TACCTGG 5100 0.0 35.4061 2 GTACCTG 5845 0.0 31.216496 1 TCAACGC 21400 0.0 28.985344 4 ATCAACG 21605 0.0 28.666815 3 ACCTGGG 6255 0.0 28.397676 3 CAACGCA 21870 0.0 28.383917 5 TATCAAC 22310 0.0 27.885426 2 AACGCAG 22605 0.0 27.585747 6 GTACATG 20990 0.0 25.495691 1 TACATGG 21610 0.0 24.32787 2 ACGCAGA 25820 0.0 24.005283 7 CATGGGG 12520 0.0 23.570763 4 CGCAGAG 26240 0.0 23.56733 8 ACATGGG 21425 0.0 23.534079 3 ATGGGGG 7370 0.0 22.953733 5 GCAGAGT 29295 0.0 20.564058 9 GAGTACT 16870 0.0 19.97222 12-13 TGGGGGG 6725 0.0 19.844692 6 >>END_MODULE