FastQCFastQC Report
Mon 6 Jun 2016
ERR1211468_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211468_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4672757
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135090.289101273616411No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119510.25575907328371666No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79590.17032771017196058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA73850.047.173031
TACCTGG52400.042.263642
GTACCTG57950.039.2379461
GTATCAA127050.036.980131
ACCTGGG64400.033.199273
TCAACGC153100.029.9852984
ATCAACG153750.029.859493
CAACGCA155900.029.4442335
AACGCAG162150.028.422186
TATCAAC162700.028.2058732
GTACATG155900.028.1739731
TAACGCA4950.026.5791974
TACATGG162000.026.4701942
ACATGGG159150.025.5099753
CATGGGG97200.025.1377324
ACGCAGA183950.024.9027527
CGCAGAG187650.024.1117948
ATGGGGG62300.023.3035585
TATAACG4850.022.2978732
CCTGGGG60900.021.2179974
Produced by FastQC (version 0.11.5)