FastQCFastQC Report
Mon 6 Jun 2016
ERR1211468_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211468_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4672757
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148830.3185057558096858No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138530.29646309448576075No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99320.21255117696041118No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA85350.045.837971
TACCTGG54400.042.0072632
GTACCTG61650.037.6790121
ACCTGGG62750.036.0991673
GTATCAA137150.034.3197331
TCAACGC169100.027.0138554
ATCAACG170500.026.7644753
GTACATG162850.026.6802481
CAACGCA172450.026.5702725
AACGCAG179250.025.6925726
TATCAAC179850.025.6213872
TACATGG166550.025.3241482
ACATGGG164750.024.3895973
CCTGGGG59800.024.1268754
CATGGGG97550.023.606564
TATAACG4400.023.5102392
ACGCAGA205200.022.2370877
CGCAGAG209650.021.653018
ATGGGGG64800.021.104385
TATCACG4850.020.359382
Produced by FastQC (version 0.11.5)