##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211468_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4672757 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06020064813985 33.0 31.0 34.0 30.0 34.0 2 32.291012564958976 34.0 31.0 34.0 30.0 34.0 3 32.30138823824992 34.0 31.0 34.0 30.0 34.0 4 35.9579877575487 37.0 35.0 37.0 35.0 37.0 5 35.878716997267354 37.0 35.0 37.0 35.0 37.0 6 35.89512272947213 37.0 35.0 37.0 35.0 37.0 7 35.8180316245848 37.0 35.0 37.0 35.0 37.0 8 35.809216486113016 37.0 35.0 37.0 35.0 37.0 9 37.4498044730338 39.0 37.0 39.0 34.0 39.0 10-11 37.405207354031035 39.0 37.0 39.0 34.0 39.0 12-13 37.31366888969403 39.0 37.0 39.0 34.0 39.0 14-15 38.52840389517366 40.0 38.0 41.0 34.0 41.0 16-17 38.45114254817874 40.0 38.0 41.0 34.0 41.0 18-19 38.43039483970598 40.0 38.0 41.0 34.0 41.0 20-21 38.366527940571274 40.0 38.0 41.0 34.0 41.0 22-23 38.25182574227592 40.0 38.0 41.0 33.5 41.0 24-25 38.13718464281365 40.0 37.5 41.0 33.0 41.0 26-27 37.956484790456685 40.0 37.0 41.0 33.0 41.0 28-29 37.99151528744166 40.0 37.0 41.0 33.0 41.0 30-31 37.91036672782257 40.0 37.0 41.0 33.0 41.0 32-33 37.7239827579307 40.0 37.0 41.0 33.0 41.0 34-35 37.603611208543484 40.0 37.0 41.0 32.0 41.0 36-37 37.54744800553506 40.0 37.0 41.0 32.0 41.0 38-39 37.44904903036901 40.0 36.5 41.0 32.0 41.0 40-41 37.282933608574126 40.0 36.0 41.0 31.0 41.0 42-43 37.12481956583662 39.0 36.0 41.0 31.0 41.0 44-45 36.94057769321195 39.0 36.0 41.0 30.5 41.0 46-47 36.67757707066727 39.0 35.0 41.0 30.0 41.0 48-49 36.45375738562908 39.0 35.0 40.5 29.5 41.0 50-51 36.253327746339046 39.0 35.0 40.0 29.0 41.0 52-53 36.04645715580759 39.0 35.0 40.0 28.0 41.0 54-55 35.84496861274832 38.0 35.0 40.0 28.0 41.0 56-57 35.63943064019807 38.0 34.0 40.0 27.5 41.0 58-59 35.36980181079393 38.0 34.0 40.0 27.0 41.0 60-61 35.04370139512926 38.0 34.0 40.0 26.0 41.0 62-63 34.708940460631695 37.0 33.0 40.0 26.0 41.0 64-65 34.38935407940109 36.5 33.0 39.5 26.0 41.0 66-67 34.0396905937972 36.0 32.5 39.0 25.0 40.5 68-69 33.63754074949757 35.5 32.0 39.0 24.5 40.0 70-71 33.24736884456007 35.0 31.5 38.0 24.0 40.0 72-73 32.831613007053434 35.0 31.0 37.5 23.0 40.0 74-75 32.42059655573786 35.0 31.0 37.0 22.0 39.0 76-77 30.89285608046813 33.5 29.5 35.5 20.5 38.0 78-79 31.745305287649238 34.0 30.5 36.0 22.0 38.5 80-81 31.727213505859602 34.0 31.0 36.0 22.0 37.5 82-83 31.44663279087699 34.0 30.5 35.0 21.0 37.0 84-85 31.143622811971603 34.0 30.0 35.0 20.0 36.5 86-87 30.84511274607261 34.0 30.0 35.0 20.0 36.0 88-89 30.563889797821716 34.0 29.5 35.0 19.5 36.0 90-91 30.268586404129294 34.0 29.0 35.0 19.0 35.5 92-93 30.054770449223017 34.0 29.0 35.0 18.0 35.0 94-95 29.828842265925662 34.0 29.0 35.0 18.0 35.0 96-97 29.615272739412728 33.5 29.0 35.0 17.5 35.0 98-99 29.445026351680603 33.0 28.5 35.0 17.0 35.0 100 27.52622338375396 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22363426273557252 1101 2 0.19003662820094291 1101 3 0.24556159084895768 1101 4 0.18638822591552895 1101 5 0.2265853258603201 1101 6 0.17829525493728227 1101 7 0.16235802501594776 1101 8 0.15304162704973123 1101 9 0.2108724203175072 1101 10-11 0.1872439999236164 1101 12-13 0.15500888598757 1101 14-15 0.23070058998084875 1101 16-17 0.29386880400002013 1101 18-19 0.262296103248147 1101 20-21 0.2588457059403879 1101 22-23 0.229754302289507 1101 24-25 0.18919009725967584 1101 26-27 0.15189434252558698 1101 28-29 0.31561997898173644 1101 30-31 0.34368515096586805 1101 32-33 0.37117915848708805 1101 34-35 0.36210090961855457 1101 36-37 0.3257622459148237 1101 38-39 0.2557553189804267 1101 40-41 0.2512336865961231 1101 42-43 0.3167100632407909 1101 44-45 0.2639727993748622 1101 46-47 0.29436432026259496 1101 48-49 0.259581280783479 1101 50-51 0.32789970214225406 1101 52-53 0.31253212389503915 1101 54-55 0.2636029437340639 1101 56-57 0.16269896513801996 1101 58-59 0.21388508140815077 1101 60-61 0.26229693273233323 1101 62-63 0.21926415099549246 1101 64-65 0.16083312377470094 1101 66-67 0.10831364020549472 1101 68-69 0.09306608098898295 1101 70-71 0.058256867138943846 1101 72-73 -0.01702924337965328 1101 74-75 0.027050096953828984 1101 76-77 0.11261116342608801 1101 78-79 0.2544454885102816 1101 80-81 0.1657076468593246 1101 82-83 0.1416730849647827 1101 84-85 0.12236309826702296 1101 86-87 -0.0012483555710289806 1101 88-89 0.020556585817047335 1101 90-91 -0.11238500361536907 1101 92-93 -0.06523209504553762 1101 94-95 -0.03505662964714773 1101 96-97 -0.12716521921573687 1101 98-99 -0.30359129803878204 1101 100 -0.2760097434510129 1102 1 0.28073853283434147 1102 2 0.26189447578926917 1102 3 0.35219498607214206 1102 4 0.1901509709338427 1102 5 0.19431639260950817 1102 6 0.1944367989912621 1102 7 0.15638117423073084 1102 8 0.18919795088274327 1102 9 0.25205838652956203 1102 10-11 0.22146694344122153 1102 12-13 0.23308259815909338 1102 14-15 0.30934446808042537 1102 16-17 0.27684190374808537 1102 18-19 0.27347096768527734 1102 20-21 0.3381472955007254 1102 22-23 0.2524808854123961 1102 24-25 0.24757301023892353 1102 26-27 0.20870529462043663 1102 28-29 0.2857793001810407 1102 30-31 0.30454904147352835 1102 32-33 0.38899340384497094 1102 34-35 0.28473625791085766 1102 36-37 0.3616135815159396 1102 38-39 0.3375367190463905 1102 40-41 0.37466238451490597 1102 42-43 0.301029530837468 1102 44-45 0.3691967510495786 1102 46-47 0.3616541206339292 1102 48-49 0.30546272557275245 1102 50-51 0.3168261992819126 1102 52-53 0.29801097217152517 1102 54-55 0.28506321300762494 1102 56-57 0.2656685864052406 1102 58-59 0.3167111776943088 1102 60-61 0.28915477655608157 1102 62-63 0.38546602259889795 1102 64-65 0.30210149685457566 1102 66-67 0.2595321437660445 1102 68-69 0.20601022727792895 1102 70-71 0.31142868451718897 1102 72-73 0.23450247404121427 1102 74-75 0.26373378333038033 1102 76-77 0.27590496480332405 1102 78-79 0.2755243483817189 1102 80-81 0.3056331531589862 1102 82-83 0.17912469116460983 1102 84-85 0.2129136205661908 1102 86-87 0.11569316378724537 1102 88-89 0.08488186594016867 1102 90-91 0.11871216345561209 1102 92-93 0.003386925468465307 1102 94-95 0.07406557345095521 1102 96-97 0.04842183089697372 1102 98-99 0.02826400092234138 1102 100 0.307430767750148 1103 1 0.24902505867238034 1103 2 0.19741169353549992 1103 3 0.25677128178179487 1103 4 0.12997833656536528 1103 5 0.1333491223190677 1103 6 0.17701001819959572 1103 7 0.12999217957653286 1103 8 0.1189014188003199 1103 9 0.155456245162803 1103 10-11 0.2018264524194251 1103 12-13 0.2326428260029516 1103 14-15 0.2501878787324259 1103 16-17 0.3094889894484467 1103 18-19 0.3426627388057639 1103 20-21 0.27104472768824195 1103 22-23 0.2907393495985744 1103 24-25 0.2430663927150576 1103 26-27 0.22614483070839952 1103 28-29 0.21447586120086015 1103 30-31 0.3491647834129452 1103 32-33 0.40323916604055654 1103 34-35 0.3687003755489613 1103 36-37 0.34861136552118666 1103 38-39 0.3405060377993365 1103 40-41 0.39544856767334124 1103 42-43 0.3666675241057149 1103 44-45 0.3150946858338415 1103 46-47 0.4766389285049044 1103 48-49 0.5306087451588013 1103 50-51 0.37706051597879053 1103 52-53 0.3436703638771661 1103 54-55 0.4543667357539434 1103 56-57 0.4847227145521913 1103 58-59 0.46084812007844533 1103 60-61 0.44403700899945875 1103 62-63 0.47670579932410106 1103 64-65 0.48685568135326207 1103 66-67 0.45788747205309477 1103 68-69 0.5349950199186964 1103 70-71 0.51745832249523 1103 72-73 0.551642736609935 1103 74-75 0.48369962959669976 1103 76-77 0.4501196573816486 1103 78-79 0.559696167934252 1103 80-81 0.5064434000406095 1103 82-83 0.46007817811993945 1103 84-85 0.4565494892209081 1103 86-87 0.45720803608197613 1103 88-89 0.31824857880511814 1103 90-91 0.3057207989815893 1103 92-93 0.2698846637830883 1103 94-95 0.2908934264490206 1103 96-97 0.3437069403259905 1103 98-99 0.45257121699325253 1103 100 0.4931992838713164 1104 1 0.22020576394984914 1104 2 0.21549207726163644 1104 3 0.26138798684571896 1104 4 0.07034924992719738 1104 5 0.10650348749132377 1104 6 0.11181066048092703 1104 7 0.149410101853924 1104 8 0.1524552796749532 1104 9 0.18410552423897286 1104 10-11 0.2523818779308087 1104 12-13 0.2333267899136473 1104 14-15 0.32439026305814167 1104 16-17 0.274683008511559 1104 18-19 0.2815868765102252 1104 20-21 0.186707316443389 1104 22-23 0.22898568337953407 1104 24-25 0.26870959804384853 1104 26-27 0.35469041666691936 1104 28-29 0.37918036661548626 1104 30-31 0.31042092800016263 1104 32-33 0.2889295158046252 1104 34-35 0.3907755109660158 1104 36-37 0.28447624825555806 1104 38-39 0.38478197237206047 1104 40-41 0.5204470276765463 1104 42-43 0.514754232085366 1104 44-45 0.45268025832250913 1104 46-47 0.3609252118560988 1104 48-49 0.39527485331996104 1104 50-51 0.452002920098586 1104 52-53 0.5081826776915506 1104 54-55 0.496024675772091 1104 56-57 0.43981127463464986 1104 58-59 0.4560193061223785 1104 60-61 0.41647866365415354 1104 62-63 0.4003895127286441 1104 64-65 0.4163160254090883 1104 66-67 0.42521058802102374 1104 68-69 0.44703081355091 1104 70-71 0.356343857613318 1104 72-73 0.41469533879385523 1104 74-75 0.43122947473461437 1104 76-77 0.38379959266071495 1104 78-79 0.45882658307631985 1104 80-81 0.580682128290718 1104 82-83 0.3839596271131924 1104 84-85 0.4386923023638545 1104 86-87 0.5215174809850005 1104 88-89 0.5736948288102539 1104 90-91 0.3796233972641829 1104 92-93 0.43851554503085666 1104 94-95 0.46869981394506866 1104 96-97 0.4297167880921755 1104 98-99 0.4735909412722279 1104 100 0.647988673909385 1105 1 0.21347731958435645 1105 2 0.1504308984905265 1105 3 0.2131391610830562 1105 4 0.1010797790131619 1105 5 0.10297535122329293 1105 6 0.12926149303565637 1105 7 0.14706343525957521 1105 8 0.15822159751708398 1105 9 0.11105080755890384 1105 10-11 0.14913018635552788 1105 12-13 0.21027914736093578 1105 14-15 0.29005627971871917 1105 16-17 0.30457752491019363 1105 18-19 0.31568569596718987 1105 20-21 0.23114865701086273 1105 22-23 0.2660250684141161 1105 24-25 0.3035315379639414 1105 26-27 0.34741451846745264 1105 28-29 0.344951591144671 1105 30-31 0.4067222329393516 1105 32-33 0.44855109745294186 1105 34-35 0.46002500255548995 1105 36-37 0.39416466119655524 1105 38-39 0.43623014568773044 1105 40-41 0.45826556116700345 1105 42-43 0.42170420060210745 1105 44-45 0.45495844374782024 1105 46-47 0.5285175608596404 1105 48-49 0.5848824856027477 1105 50-51 0.5901979192103681 1105 52-53 0.5098302939366377 1105 54-55 0.5147213909812862 1105 56-57 0.6019085748493112 1105 58-59 0.6133094768823142 1105 60-61 0.6904419875809324 1105 62-63 0.5932989318049167 1105 64-65 0.5530315035545499 1105 66-67 0.5683791167878596 1105 68-69 0.6455116164485446 1105 70-71 0.7354390541226294 1105 72-73 0.5882995660030801 1105 74-75 0.6019045498943925 1105 76-77 0.4782368198378393 1105 78-79 0.6210712421101654 1105 80-81 0.7444229765622055 1105 82-83 0.7636057806185903 1105 84-85 0.6329268207604315 1105 86-87 0.5970115850501578 1105 88-89 0.6038389737607055 1105 90-91 0.6342831678997314 1105 92-93 0.6722847169330564 1105 94-95 0.7547642463627433 1105 96-97 0.6587771450892568 1105 98-99 0.7373354848147251 1105 100 0.8359298180737724 1106 1 0.2504266137027784 1106 2 0.17347192003744993 1106 3 0.23574837245331537 1106 4 0.12721324542021506 1106 5 0.17079329031198398 1106 6 0.10349826959807018 1106 7 0.19196535751527222 1106 8 0.15207842692728946 1106 9 0.10422108436170419 1106 10-11 0.12986413540673425 1106 12-13 0.2511964918775931 1106 14-15 0.25612238557034317 1106 16-17 0.29905743125170403 1106 18-19 0.27405403062041955 1106 20-21 0.28434898380182005 1106 22-23 0.24747898736342222 1106 24-25 0.22535975423408416 1106 26-27 0.26093677266543125 1106 28-29 0.37166803755112454 1106 30-31 0.34557712181837275 1106 32-33 0.4387206122422711 1106 34-35 0.4856720384055393 1106 36-37 0.41661432456553626 1106 38-39 0.44134638823916106 1106 40-41 0.46297394482665766 1106 42-43 0.4066187653192799 1106 44-45 0.498320002573017 1106 46-47 0.609797324386598 1106 48-49 0.5682116279383536 1106 50-51 0.6120183603781513 1106 52-53 0.676071143019044 1106 54-55 0.6272451762293727 1106 56-57 0.6470826829416296 1106 58-59 0.7622817358362042 1106 60-61 0.8616702766477076 1106 62-63 0.7706267638620901 1106 64-65 0.7307537588823436 1106 66-67 0.7162403223936238 1106 68-69 0.6740581127820704 1106 70-71 0.70466100417174 1106 72-73 0.622120745977341 1106 74-75 0.6798721083656858 1106 76-77 0.6300876987946502 1106 78-79 0.6668917867932969 1106 80-81 0.7055978101780767 1106 82-83 0.7227704620958946 1106 84-85 0.7852753902408338 1106 86-87 0.660936991855575 1106 88-89 0.8001375212021102 1106 90-91 0.7898131550610756 1106 92-93 0.8256682109365698 1106 94-95 0.8044195730597963 1106 96-97 0.7240712485462844 1106 98-99 0.9139889593808661 1106 100 0.9387669403286125 1107 1 0.24266107420680783 1107 2 0.1950799402822625 1107 3 0.2487631967359505 1107 4 0.16850482106877962 1107 5 0.14967540583144512 1107 6 0.1657046510731348 1107 7 0.1514737770919936 1107 8 0.17495725737788348 1107 9 0.2109397587524029 1107 10-11 0.18601153821898464 1107 12-13 0.24279665678908202 1107 14-15 0.2212600781010039 1107 16-17 0.18232167652296738 1107 18-19 0.21217964142500279 1107 20-21 0.2673586308795777 1107 22-23 0.2943289994685614 1107 24-25 0.33419365272903434 1107 26-27 0.3220121039805335 1107 28-29 0.38533276708923125 1107 30-31 0.24507727209962837 1107 32-33 0.22679730511787 1107 34-35 0.31745670570700213 1107 36-37 0.36222415660250107 1107 38-39 0.3548603238422672 1107 40-41 0.27532502175566975 1107 42-43 0.287784333494173 1107 44-45 0.4042131706520493 1107 46-47 0.3522211668306241 1107 48-49 0.3738663890232772 1107 50-51 0.3095626045904041 1107 52-53 0.34815853295178556 1107 54-55 0.312683572082463 1107 56-57 0.42902129671786327 1107 58-59 0.5129901199391966 1107 60-61 0.5708638630963137 1107 62-63 0.4490239009274788 1107 64-65 0.4918266148415853 1107 66-67 0.5874727312982273 1107 68-69 0.5258377444368705 1107 70-71 0.5437525049551297 1107 72-73 0.6121317668584751 1107 74-75 0.6397882729980466 1107 76-77 0.3929021799401653 1107 78-79 0.5933177448057556 1107 80-81 0.5482062866229427 1107 82-83 0.578337751679932 1107 84-85 0.4820518532871034 1107 86-87 0.38638787008276054 1107 88-89 0.48956407115747425 1107 90-91 0.36004781956198073 1107 92-93 0.4193415112508774 1107 94-95 0.5986547753288924 1107 96-97 0.6226088603351947 1107 98-99 0.628940808926977 1107 100 0.8930800967952841 1108 1 0.24452163265459603 1108 2 0.1537690856607412 1108 3 0.1941290082878524 1108 4 0.11497461403862985 1108 5 0.12312649120391939 1108 6 0.11593748712157748 1108 7 0.1587544717662439 1108 8 0.16913613196254573 1108 9 0.1123213049541576 1108 10-11 0.16690065355410155 1108 12-13 0.21187934777742612 1108 14-15 0.22180836944089322 1108 16-17 0.3159414307055002 1108 18-19 0.19732605796947666 1108 20-21 0.2763779169237637 1108 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82-83 -0.08503473907230585 2316 84-85 -0.09533288483523705 2316 86-87 -0.05011840227051678 2316 88-89 -0.04386687088046415 2316 90-91 0.04123052008100103 2316 92-93 0.12511792005517464 2316 94-95 0.1088918437863633 2316 96-97 0.21632920723191162 2316 98-99 0.025605057770555106 2316 100 -0.09374025289878318 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 3.0 10 4.0 11 24.0 12 62.0 13 174.0 14 512.0 15 1312.0 16 2991.0 17 6092.0 18 10931.0 19 16263.0 20 22559.0 21 30600.0 22 39015.0 23 45312.0 24 49228.0 25 54671.0 26 63593.0 27 76030.0 28 91055.0 29 109640.0 30 132203.0 31 159499.0 32 194981.0 33 246012.0 34 323086.0 35 436315.0 36 632906.0 37 946956.0 38 861362.0 39 119357.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.1981428322867 19.963814570863025 13.787701448380227 24.050341148470046 2 15.084302415844808 21.278257396737974 40.005781838389055 23.63165834902817 3 17.171136756389473 27.218271993322958 30.925081189241823 24.685510061045747 4 11.985778735773012 17.54005856544827 37.91512482769676 32.55903787108197 5 12.374515374889464 38.210052295425925 35.28278394173456 14.132648387950052 6 30.385646408541824 37.79113091587224 18.346143129299687 13.477079546286259 7 26.760240260728303 32.90121870236351 22.859074417950687 17.479466618957503 8 25.885829446760134 36.277270304112534 20.3575183656768 17.47938188345053 9 26.76550632999433 15.213301452056207 20.362578548448848 37.65861366950061 10-11 24.738853315077158 26.321602000703226 29.10674576058631 19.83279892363331 12-13 25.150612368672288 24.14774618068091 28.962633836940377 21.739007613706427 14-15 22.538653304676448 25.529435834133896 26.501988012644357 25.4299228485453 16-17 22.15385859922651 28.24742219503371 27.22110468504944 22.377614520690333 18-19 22.018235238601317 27.438225903549352 29.00709031652075 21.53644854132858 20-21 22.83940035585894 26.769953075757208 28.907343132508874 21.48330343587498 22-23 22.552424617843382 26.682834138389822 28.74546012129456 22.019281122472236 24-25 22.24176933717161 26.7349639843129 28.869443308129433 22.15382337038606 26-27 22.215843630360162 26.816348023010438 28.809168736558366 22.15863961007103 28-29 22.19182116100761 27.01598449039392 28.854165574332878 21.938028774265593 30-31 22.64690873359095 26.780300034979867 28.898523836973084 21.674267394456102 32-33 22.404701257666467 26.884295369922928 28.792174928765906 21.918828443644703 34-35 22.560017241032458 27.092743742528206 28.707763643764654 21.639475372674678 36-37 22.57158877264619 27.01124297648359 28.588812237905536 21.82835601296468 38-39 22.506439537260718 27.109082208643574 28.54786615692525 21.836612097170455 40-41 22.570768602875066 26.946686336293695 28.64894873576704 21.8335963250642 42-43 22.38525347831363 27.02577298295905 28.48780008607902 22.101173452648297 44-45 22.26795415193024 27.201748435720813 28.440448456127363 22.089848956221584 46-47 22.32136350547084 27.02415742126224 28.238989490859893 22.415489582407027 48-49 22.314211961738565 27.17886234020727 28.244999712138203 22.26192598591596 50-51 22.328859593307644 27.346680287142927 28.140762185928065 22.183697933621367 52-53 22.378347930525866 27.418841549907675 27.896050325857964 22.3067601937085 54-55 22.260462478465108 27.335034723336864 28.078905974126034 22.325596824071994 56-57 22.42478607721027 27.387285452565465 27.815874720488164 22.372053749736097 58-59 22.370596795147748 27.37849009557458 28.018557415213497 22.23235569406417 60-61 22.604501218901323 27.46398005070364 27.951529878732668 21.97998885166237 62-63 22.4317877523222 27.421528505274846 28.02251822590848 22.12416551649447 64-65 22.307172829084205 27.214424907671546 28.0033673013297 22.47503496191455 66-67 22.277510302752717 27.29259389657545 27.898484577707528 22.531411222964305 68-69 22.20129226157333 27.33118138331725 27.994456140703843 22.47307021440558 70-71 22.255740973425624 27.187502474580572 27.959421801006695 22.597334750987102 72-73 22.127567408381445 27.483854177367768 27.709101672453823 22.679476741796964 74-75 22.211038908230815 27.486583590076354 27.624514299851306 22.677863201841532 76-77 22.056708583139333 27.37323723319607 27.78357394749762 22.786480236166987 78-79 22.008084645326985 27.280782246953546 27.85431586832355 22.856817239395927 80-81 22.063490670683905 27.472606376546832 27.55428591240418 22.90961704036508 82-83 22.194982146977402 27.498748939970096 27.56875051101896 22.737518402033547 84-85 22.125094245827366 27.519460525914457 27.46486884780095 22.89057638045723 86-87 21.90916668343406 27.484970351682854 27.63209886796556 22.973764096917527 88-89 21.842089716322988 27.33987300804488 27.706070818221356 23.111966457410777 90-91 22.153433847319633 27.32678247858628 27.595509514294186 22.9242741597999 92-93 21.981376892292452 27.354969380367212 27.582192188450644 23.081461538889695 94-95 21.801663687118666 27.2231137138313 27.60253910797246 23.372683491077574 96-97 21.74231938008988 27.1942529816976 27.666970730949775 23.396456907262746 98-99 21.64246288845571 27.107111430845805 27.590113205915586 23.6603124747829 100 20.808798316057683 27.599192104957858 27.79979597139832 23.79221360758613 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1167.0 1 891.5 2 1350.0 3 1955.0 4 1435.0 5 1161.5 6 1673.5 7 3346.0 8 5276.5 9 6203.0 10 5557.0 11 4480.0 12 4461.0 13 4833.5 14 5237.5 15 5530.5 16 5996.0 17 6389.0 18 6691.5 19 7196.0 20 7955.0 21 9163.0 22 10924.5 23 13246.0 24 16281.5 25 20442.0 26 25386.0 27 31293.5 28 38249.0 29 46265.0 30 55267.0 31 64312.5 32 74125.0 33 84330.5 34 94640.0 35 104042.0 36 115088.5 37 126128.5 38 134211.0 39 140584.5 40 144421.5 41 148329.5 42 152078.0 43 156123.5 44 160966.5 45 166031.0 46 170246.0 47 173498.5 48 177546.0 49 180914.0 50 182435.0 51 177899.0 52 169292.0 53 161656.5 54 152947.5 55 142324.0 56 129854.5 57 116450.5 58 102628.5 59 87533.0 60 72032.0 61 58623.5 62 47513.5 63 36891.5 64 27450.5 65 20589.0 66 15391.0 67 11632.5 68 8982.5 69 6830.0 70 5273.0 71 4040.5 72 3081.5 73 2421.5 74 1871.0 75 1463.0 76 1062.5 77 696.5 78 467.0 79 312.5 80 222.5 81 165.5 82 113.5 83 74.0 84 47.0 85 37.0 86 28.5 87 18.0 88 16.5 89 12.5 90 4.5 91 2.0 92 3.0 93 4.0 94 4.5 95 4.5 96 3.5 97 2.0 98 3.0 99 3.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0033813014458059774 2 0.06338870178783103 3 6.848205459860207E-4 4 7.704231142342733E-4 5 0.0015622468705306098 6 6.634199039239575E-4 7 0.0 8 9.844295348549047E-4 9 8.560256824825258E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.741202249549892E-4 18-19 9.844295348549047E-4 20-21 0.0025466764053855145 22-23 0.0 24-25 3.2100963093094717E-5 26-27 1.8190545752753675E-4 28-29 8.56025682482526E-4 30-31 0.0014659439812513256 32-33 0.0028890866783785247 34-35 0.003916317497357556 36-37 0.001326839807847915 38-39 0.0013054391657858519 40-41 0.0051361540948951555 42-43 0.0065485964709913235 44-45 0.005082652489739997 46-47 0.006430892939649975 48-49 0.008046641415335743 50-51 0.008132243983583996 52-53 0.0036167085084886715 54-55 0.002289868700640757 56-57 0.0021721651692994093 58-59 0.002642979294664799 60-61 0.00666630000233267 62-63 0.013493104820130813 64-65 0.0036916107557058925 66-67 0.009887096632673174 68-69 0.011995059875786394 70-71 0.00530735923139166 72-73 0.007297618943163532 74-75 0.002589477689509641 76-77 0.0028034841101302723 78-79 0.006837505138829175 80-81 0.004879346390150398 82-83 0.015387061642623403 84-85 0.017109813328619487 86-87 0.021208036283504577 88-89 0.020651619589890934 90-91 0.023583507552393586 92-93 0.0336204086795012 94-95 0.037376221361393284 96-97 0.022417172560011148 98-99 0.017719731627388286 100 0.018468754099560496 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4672757.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.1412847231293 #Duplication Level Percentage of deduplicated Percentage of total 1 78.6782781129117 52.82560672304557 2 14.071755910691325 18.895915403082082 3 3.7042949766590048 7.461333711789594 4 1.3797395451696781 3.705499425839931 5 0.654669037803483 2.1977660133290375 6 0.3987897580799608 1.60651540151487 7 0.2376584973680844 1.1169687787063143 8 0.17051339414182035 0.9158790676146502 9 0.11595299767415612 0.7006709908206781 >10 0.5495942172971694 6.245571230750537 >50 0.025861517971311594 1.1829932935084604 >100 0.01177006091002079 1.4276874500445231 >500 6.96397264341552E-4 0.3122069190533051 >1k 3.0950986022152077E-4 0.4009412896798463 >5k 3.8688732527690097E-5 0.25799474500163483 >10k+ 7.737746505538019E-5 0.746449556218987 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14883 0.3185057558096858 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13853 0.29646309448576075 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9932 0.21255117696041118 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.1400642062063145E-5 14-15 0.0 0.0 0.0 1.0700321031031573E-5 4.280128412412629E-5 16-17 0.0 0.0 0.0 4.280128412412629E-5 6.420192618618943E-5 18-19 0.0 0.0 0.0 6.420192618618943E-5 1.0700321031031573E-4 20-21 0.0 0.0 0.0 8.560256824825258E-5 1.0700321031031573E-4 22-23 0.0 0.0 0.0 1.6050481546547358E-4 1.177035313413473E-4 24-25 0.0 0.0 0.0 2.461073837137262E-4 1.2840385237237887E-4 26-27 0.0 0.0 0.0 3.7451123608610504E-4 1.6050481546547358E-4 28-29 0.0 0.0 0.0 9.951298558859363E-4 1.8190545752753675E-4 30-31 0.0 4.280128412412629E-5 0.0 0.0033920017668370083 1.9260577855856833E-4 32-33 0.0 4.280128412412629E-5 0.0 0.010176005300511026 2.5680770474475773E-4 34-35 0.0 4.280128412412629E-5 0.0 0.02091912761566673 2.782083468068209E-4 36-37 0.0 4.280128412412629E-5 0.0 0.039045471442234206 2.782083468068209E-4 38-39 0.0 4.280128412412629E-5 0.0 0.07030110917387744 2.782083468068209E-4 40-41 0.0 4.280128412412629E-5 0.0 0.10654309650598137 2.9960898886888406E-4 42-43 0.0 4.280128412412629E-5 0.0 0.14238917195993714 2.9960898886888406E-4 44-45 0.0 4.280128412412629E-5 0.0 0.18803674147831784 2.9960898886888406E-4 46-47 0.0 4.280128412412629E-5 0.0 0.24144204374419642 2.9960898886888406E-4 48-49 0.0 4.280128412412629E-5 0.0 0.3021449649532385 3.2100963093094717E-4 50-51 0.0 4.280128412412629E-5 0.0 0.3685511572718205 3.2100963093094717E-4 52-53 0.0 4.280128412412629E-5 0.0 0.4398045950174597 3.638109150550735E-4 54-55 0.0 6.420192618618943E-5 0.0 0.5230744932809475 4.0661219917919976E-4 56-57 0.0 8.560256824825258E-5 0.0 0.6178579369738251 4.280128412412629E-4 58-59 0.0 1.0700321031031573E-4 0.0 0.7302434087627497 4.494134833033261E-4 60-61 0.0 1.0700321031031573E-4 0.0 0.847615230152135 4.494134833033261E-4 62-63 0.0 1.0700321031031573E-4 0.0 0.9704549155883775 4.708141253653892E-4 64-65 0.0 1.0700321031031573E-4 0.0 1.0950494536737092 4.708141253653892E-4 66-67 0.0 1.0700321031031573E-4 0.0 1.2461058856687819 4.708141253653892E-4 68-69 0.0 1.0700321031031573E-4 0.0 1.4220726650240962 4.708141253653892E-4 70-71 0.0 1.2840385237237887E-4 0.0 1.6145179387671988 4.708141253653892E-4 72-73 0.0 1.4980449443444203E-4 0.0 1.8027579863451062 5.029150884584839E-4 74-75 0.0 1.4980449443444203E-4 0.0 1.9903239136980586 5.136154094895155E-4 76-77 0.0 1.4980449443444203E-4 0.0 2.2020511659390807 5.136154094895155E-4 78-79 0.0 1.4980449443444203E-4 0.0 2.449517490423748 5.136154094895155E-4 80-81 0.0 1.4980449443444203E-4 0.0 2.714008025668786 5.136154094895155E-4 82-83 0.0 1.4980449443444203E-4 0.0 2.993928423840572 5.350160515515787E-4 84-85 0.0 1.4980449443444203E-4 0.0 3.280622125225001 5.350160515515787E-4 86-87 0.0 1.4980449443444203E-4 0.0 3.5810657391343055 5.457163725826103E-4 88 0.0 1.4980449443444203E-4 0.0 3.8214912523805538 5.564166936136418E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8535 0.0 45.83797 1 TACCTGG 5440 0.0 42.007263 2 GTACCTG 6165 0.0 37.679012 1 ACCTGGG 6275 0.0 36.099167 3 GTATCAA 13715 0.0 34.319733 1 TCAACGC 16910 0.0 27.013855 4 ATCAACG 17050 0.0 26.764475 3 GTACATG 16285 0.0 26.680248 1 CAACGCA 17245 0.0 26.570272 5 AACGCAG 17925 0.0 25.692572 6 TATCAAC 17985 0.0 25.621387 2 TACATGG 16655 0.0 25.324148 2 ACATGGG 16475 0.0 24.389597 3 CCTGGGG 5980 0.0 24.126875 4 CATGGGG 9755 0.0 23.60656 4 TATAACG 440 0.0 23.510239 2 ACGCAGA 20520 0.0 22.237087 7 CGCAGAG 20965 0.0 21.65301 8 ATGGGGG 6480 0.0 21.10438 5 TATCACG 485 0.0 20.35938 2 >>END_MODULE