FastQCFastQC Report
Mon 6 Jun 2016
ERR1211467_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211467_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5022020
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT192100.3825154021688484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160620.31983146224029374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112760.22453116475043908No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66340.1320982393538855No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG69850.057.0762862
GTACCTG78700.051.431671
ACCTGGG80300.048.2251473
GGTATCA103400.041.0120161
TATAACG6450.040.818272
TAACGCA7050.039.9958884
GTATCAA170050.035.095351
ATAACGC8850.031.8617673
CCTGGGG59550.031.8036164
TCAACGC200850.028.8265134
ATCAACG201950.028.6933383
CAACGCA202750.028.62425
CATGGGG94300.027.808384
TATCAAC209700.027.7106232
AACGCAG210750.027.6444426
GTACATG200950.027.2160431
TACATGG202100.026.6357822
ACATGGG197200.026.0242123
ATGGGGG62300.025.1179075
ACGCAGA235300.024.6812747
Produced by FastQC (version 0.11.5)