##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211467_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5022020 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.715870506290297 33.0 31.0 34.0 30.0 34.0 2 31.761137550228792 33.0 31.0 34.0 30.0 34.0 3 31.863080393945065 34.0 31.0 34.0 30.0 34.0 4 35.417713191106365 37.0 35.0 37.0 33.0 37.0 5 35.504491021541135 37.0 35.0 37.0 33.0 37.0 6 35.614571228310524 37.0 35.0 37.0 33.0 37.0 7 35.561266183726865 37.0 35.0 37.0 33.0 37.0 8 35.532409468699846 37.0 35.0 37.0 33.0 37.0 9 37.17321695254101 39.0 37.0 39.0 34.0 39.0 10-11 37.151780757543776 39.0 37.0 39.0 33.5 39.0 12-13 37.109642335155975 39.0 37.0 39.0 33.0 39.0 14-15 38.36738672884616 40.0 38.0 41.0 34.0 41.0 16-17 38.35489245363419 40.0 38.0 41.0 34.0 41.0 18-19 38.37099653127626 40.0 38.0 41.0 34.0 41.0 20-21 38.320875464454545 40.0 38.0 41.0 34.0 41.0 22-23 38.171401846269035 40.0 38.0 41.0 33.5 41.0 24-25 38.102461957539 40.0 38.0 41.0 33.0 41.0 26-27 37.970868694270436 40.0 38.0 41.0 33.0 41.0 28-29 37.81617904747492 40.0 38.0 41.0 33.0 41.0 30-31 37.639186124308544 40.0 37.0 41.0 32.0 41.0 32-33 37.463391722852556 40.0 37.0 41.0 32.0 41.0 34-35 37.294014659439824 40.0 36.5 41.0 31.0 41.0 36-37 37.12915241277415 40.0 36.0 41.0 31.0 41.0 38-39 37.00210721980398 39.0 36.0 41.0 31.0 41.0 40-41 36.96061664429851 39.0 36.0 41.0 30.5 41.0 42-43 36.90646184204762 39.0 36.0 41.0 30.0 41.0 44-45 36.73088050226801 39.0 36.0 41.0 30.0 41.0 46-47 36.66809769773916 39.0 36.0 41.0 30.0 41.0 48-49 36.62253525473814 40.0 36.0 41.0 29.5 41.0 50-51 35.66602134200979 38.5 34.5 40.0 28.0 40.5 52-53 35.876002883301936 38.5 35.0 40.0 28.5 40.5 54-55 36.465652466537364 39.0 36.0 41.0 29.0 41.0 56-57 36.44071250214057 39.0 35.0 41.0 29.0 41.0 58-59 36.29380986137052 39.0 35.0 41.0 28.5 41.0 60-61 36.112459528237636 39.0 35.0 41.0 28.0 41.0 62-63 35.86470145479309 39.0 35.0 40.5 28.0 41.0 64-65 35.59407001565107 38.0 35.0 40.0 28.0 41.0 66-67 35.301591690196375 37.5 34.0 40.0 27.5 41.0 68-69 35.016891708913946 37.0 34.0 40.0 27.0 41.0 70-71 34.67008424896755 36.5 34.0 39.0 26.0 41.0 72-73 34.311457341866415 36.0 34.0 39.0 26.0 41.0 74-75 33.93278581128709 35.5 33.5 39.0 26.0 40.0 76-77 33.574778276470425 35.0 33.0 37.5 26.0 39.5 78-79 33.23691064551714 35.0 33.0 37.0 25.5 39.0 80-81 32.87922041329983 35.0 33.0 36.5 25.0 39.0 82-83 32.55357396027893 35.0 32.5 36.0 24.5 38.0 84-85 32.26972383622527 35.0 32.0 36.0 24.0 37.0 86-87 31.965028912668608 35.0 32.0 35.0 23.5 37.0 88-89 31.731496589021948 35.0 32.0 35.0 23.0 36.0 90-91 31.491095117104273 35.0 31.5 35.0 21.5 36.0 92-93 31.323552674023603 34.0 31.0 35.0 20.5 36.0 94-95 31.15514245263858 34.0 31.0 35.0 20.0 35.5 96-97 31.045835639842135 34.0 31.0 35.0 20.0 35.0 98-99 30.86651745711885 34.0 31.0 35.0 19.5 35.0 100 29.511201667854767 33.0 29.0 35.0 17.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.46440171744161063 1101 2 0.4595864369236189 1101 3 0.4072921385095363 1101 4 0.4325433670945529 1101 5 0.3846978669652543 1101 6 0.39528564028006485 1101 7 0.4368514213248318 1101 8 0.4320979231382296 1101 9 0.4475550221718123 1101 10-11 0.4720133771432984 1101 12-13 0.426743899404471 1101 14-15 0.5746729962913761 1101 16-17 0.5834720168682068 1101 18-19 0.530264900365367 1101 20-21 0.5379905359839157 1101 22-23 0.5709656772050238 1101 24-25 0.5371257193805903 1101 26-27 0.44061397576776784 1101 28-29 0.558485883526032 1101 30-31 0.5692248779938822 1101 32-33 0.470912487181657 1101 34-35 0.5239303793952175 1101 36-37 0.4729473896610372 1101 38-39 0.5374004686372302 1101 40-41 0.4960872572180506 1101 42-43 0.447748918038144 1101 44-45 0.3809339454566114 1101 46-47 0.37035211840532867 1101 48-49 0.5053329830861983 1101 50-51 0.4404729094175366 1101 52-53 0.5639356377951472 1101 54-55 0.5948766557473277 1101 56-57 0.629235457394401 1101 58-59 0.5379462278906288 1101 60-61 0.526835494135625 1101 62-63 0.5785360651317788 1101 64-65 0.6033629915156311 1101 66-67 0.6460493809853887 1101 68-69 0.5460065780922108 1101 70-71 0.5098235781616012 1101 72-73 0.4602277325957189 1101 74-75 0.3582292661275517 1101 76-77 0.333572179990405 1101 78-79 0.31274741295262487 1101 80-81 0.27804280808695836 1101 82-83 0.2736899910442361 1101 84-85 0.18402996583846942 1101 86-87 0.15062092874247313 1101 88-89 0.21905459659729232 1101 90-91 0.11818436754867534 1101 92-93 0.15887668005288447 1101 94-95 -0.024803964236124187 1101 96-97 -0.060285826852499724 1101 98-99 -0.03882666021909742 1101 100 -0.125475173761739 1102 1 0.4128014625540217 1102 2 0.4483896372986784 1102 3 0.47255912383059595 1102 4 0.36460545081408213 1102 5 0.410438886623389 1102 6 0.4111229567933279 1102 7 0.4050566193072882 1102 8 0.4057117361057081 1102 9 0.4860284314145815 1102 10-11 0.40339169660958873 1102 12-13 0.40877095628805904 1102 14-15 0.5227405590163627 1102 16-17 0.47888171633794485 1102 18-19 0.4860197758314868 1102 20-21 0.4949913227405389 1102 22-23 0.49984233963255065 1102 24-25 0.5881564177079284 1102 26-27 0.49669035717229093 1102 28-29 0.5247472605117807 1102 30-31 0.44275435711788447 1102 32-33 0.47798619038042034 1102 34-35 0.5219763871442282 1102 36-37 0.5268052925235409 1102 38-39 0.5131866190262926 1102 40-41 0.5153869764644838 1102 42-43 0.42496392757621493 1102 44-45 0.4842036332327879 1102 46-47 0.4046281002344827 1102 48-49 0.5865817369391522 1102 50-51 0.6040076156232104 1102 52-53 0.6378510183482433 1102 54-55 0.6836301786387153 1102 56-57 0.6617756574245774 1102 58-59 0.6407588491373701 1102 60-61 0.5580362881670453 1102 62-63 0.6537148703933511 1102 64-65 0.6638665292260626 1102 66-67 0.5439913186160652 1102 68-69 0.5674991793467115 1102 70-71 0.6126581248604595 1102 72-73 0.5146658011082081 1102 74-75 0.43527362087934307 1102 76-77 0.413456436505399 1102 78-79 0.44069269834050573 1102 80-81 0.43916190875947336 1102 82-83 0.38905996873483417 1102 84-85 0.32493410277722745 1102 86-87 0.22750557459422183 1102 88-89 0.2456345089763161 1102 90-91 0.1848719605045126 1102 92-93 0.20685856174839756 1102 94-95 0.16226463312770179 1102 96-97 0.08726135160114623 1102 98-99 0.039873857639200594 1102 100 0.0979367633839523 1103 1 0.3729883470836697 1103 2 0.43449626083227244 1103 3 0.36535079529370407 1103 4 0.4242501524058042 1103 5 0.35958295116078887 1103 6 0.41674025196793707 1103 7 0.3796857019490645 1103 8 0.3934745046626915 1103 9 0.3832807585596356 1103 10-11 0.4174137266544875 1103 12-13 0.4076930001990178 1103 14-15 0.5159055842107421 1103 16-17 0.47028843235561624 1103 18-19 0.43345568123349665 1103 20-21 0.41850569112798297 1103 22-23 0.5278138644815868 1103 24-25 0.476974887966513 1103 26-27 0.45868560281102333 1103 28-29 0.4853594311272289 1103 30-31 0.44474070427194334 1103 32-33 0.3610971706068753 1103 34-35 0.4693744878851547 1103 36-37 0.5372609793125775 1103 38-39 0.4537795700921521 1103 40-41 0.330656560325437 1103 42-43 0.3228048268562276 1103 44-45 0.3670198777256033 1103 46-47 0.38187934164013626 1103 48-49 0.34920818306554935 1103 50-51 0.31944862857883294 1103 52-53 0.35483636479067826 1103 54-55 0.3942102676170194 1103 56-57 0.3519266929321816 1103 58-59 0.4409784333907467 1103 60-61 0.3768190075125659 1103 62-63 0.4595719602716386 1103 64-65 0.42911367883309737 1103 66-67 0.4706893725262944 1103 68-69 0.316802409356022 1103 70-71 0.3864523981737662 1103 72-73 0.3446658109191105 1103 74-75 0.3443698278552816 1103 76-77 0.2697839262497368 1103 78-79 0.2805897950995089 1103 80-81 0.31192340516999195 1103 82-83 0.3471904890002335 1103 84-85 0.269221249454084 1103 86-87 0.24866444443107483 1103 88-89 0.24453009616511423 1103 90-91 0.20799912544271493 1103 92-93 0.09275070269568886 1103 94-95 0.08157818747222834 1103 96-97 0.11425276771927528 1103 98-99 0.10358252759535347 1103 100 0.0483982680356263 1104 1 0.37292727205213083 1104 2 0.4213878159279254 1104 3 0.3625665442299528 1104 4 0.35288324358256773 1104 5 0.35040886773510493 1104 6 0.3430047026109193 1104 7 0.40254245309034076 1104 8 0.3929712477085161 1104 9 0.4070082614199819 1104 10-11 0.39723849909205455 1104 12-13 0.39515182514936953 1104 14-15 0.4752326326806795 1104 16-17 0.47953960219872016 1104 18-19 0.4233378074019214 1104 20-21 0.4201788564696258 1104 22-23 0.47427569095472677 1104 24-25 0.5132098783123524 1104 26-27 0.5154503072532393 1104 28-29 0.5025398603498772 1104 30-31 0.4365235826980225 1104 32-33 0.43220553063945744 1104 34-35 0.42642744623758944 1104 36-37 0.372467225874864 1104 38-39 0.4801243551299095 1104 40-41 0.4952200174790633 1104 42-43 0.26571423113800563 1104 44-45 0.36946908115506716 1104 46-47 0.3700635427601213 1104 48-49 0.3791142855090399 1104 50-51 0.45211440191571484 1104 52-53 0.5600090053962745 1104 54-55 0.5356264347099469 1104 56-57 0.5470657275810851 1104 58-59 0.5298361899360771 1104 60-61 0.5831133591330513 1104 62-63 0.4991862343976621 1104 64-65 0.41787995340306594 1104 66-67 0.42057979001379664 1104 68-69 0.38740676164568555 1104 70-71 0.4090932975533832 1104 72-73 0.5289040296565162 1104 74-75 0.46189868833752 1104 76-77 0.4929968776818683 1104 78-79 0.335399592566084 1104 80-81 0.2747084886299547 1104 82-83 0.31538113711044247 1104 84-85 0.18555033321435843 1104 86-87 0.2663092038440915 1104 88-89 0.2850358176649266 1104 90-91 0.1731409837101232 1104 92-93 0.24163072175919353 1104 94-95 0.32761423512446086 1104 96-97 0.34523153508738247 1104 98-99 0.24754486411518783 1104 100 0.2736572167028939 1105 1 0.4437868780770131 1105 2 0.46355276913432775 1105 3 0.3950885250383145 1105 4 0.40837867618463264 1105 5 0.36773257671249127 1105 6 0.3337311395136524 1105 7 0.34761420766926676 1105 8 0.3896401755719481 1105 9 0.43722889275489507 1105 10-11 0.4129823230611791 1105 12-13 0.4371341482985329 1105 14-15 0.5018826586954219 1105 16-17 0.4888293668805801 1105 18-19 0.4989880441689749 1105 20-21 0.5135468102621772 1105 22-23 0.5803763120154741 1105 24-25 0.5347009180457505 1105 26-27 0.5629485784256119 1105 28-29 0.5757881134107308 1105 30-31 0.619236426076121 1105 32-33 0.6129537672352754 1105 34-35 0.6189270675420175 1105 36-37 0.6506063487500526 1105 38-39 0.5428888328263852 1105 40-41 0.49594852897494235 1105 42-43 0.5124507973241208 1105 44-45 0.5477528657398594 1105 46-47 0.5152572702441987 1105 48-49 0.5472765582093686 1105 50-51 0.5536311270881029 1105 52-53 0.598429910709207 1105 54-55 0.6434490339446555 1105 56-57 0.5646054576863904 1105 58-59 0.5828306181716201 1105 60-61 0.6457686078537179 1105 62-63 0.6048293582739248 1105 64-65 0.6187885922723879 1105 66-67 0.6144462059730174 1105 68-69 0.6135780505937021 1105 70-71 0.5666865389899272 1105 72-73 0.5448341718484642 1105 74-75 0.6225685514143677 1105 76-77 0.6222574663394411 1105 78-79 0.5170771151725617 1105 80-81 0.5645784478625515 1105 82-83 0.5235619164988634 1105 84-85 0.43700007866507207 1105 86-87 0.5453148151246019 1105 88-89 0.5892508311333131 1105 90-91 0.46726132328479864 1105 92-93 0.4753454948382192 1105 94-95 0.5000877428992467 1105 96-97 0.5104262834030102 1105 98-99 0.5184546555362495 1105 100 0.5876225874623273 1106 1 0.3498905101148253 1106 2 0.3293053250798472 1106 3 0.3399462174313719 1106 4 0.30028383308725637 1106 5 0.2953803462573248 1106 6 0.25161747565493187 1106 7 0.2815183669967851 1106 8 0.3357669662523648 1106 9 0.316510201278561 1106 10-11 0.3559558204782718 1106 12-13 0.3478342705488586 1106 14-15 0.47796464026429675 1106 16-17 0.39958825209363624 1106 18-19 0.40512803396479313 1106 20-21 0.3900601593512931 1106 22-23 0.4516181593439086 1106 24-25 0.44824977852450587 1106 26-27 0.49401448016355687 1106 28-29 0.47889189782340935 1106 30-31 0.5021079536912083 1106 32-33 0.44698061817775425 1106 34-35 0.47316067131260553 1106 36-37 0.498519916381575 1106 38-39 0.5184798792068861 1106 40-41 0.4254972041118634 1106 42-43 0.3853619216364379 1106 44-45 0.4148743780720352 1106 46-47 0.4745107203861423 1106 48-49 0.4878657265112949 1106 50-51 0.5367243153477617 1106 52-53 0.6172083439036697 1106 54-55 0.5409352446038085 1106 56-57 0.6104939776942899 1106 58-59 0.5610789932183238 1106 60-61 0.5169672833865064 1106 62-63 0.5414119048887542 1106 64-65 0.5967169671321884 1106 66-67 0.6554187709701793 1106 68-69 0.5986629009373203 1106 70-71 0.5765620101806306 1106 72-73 0.5226290889138454 1106 74-75 0.5995423282718946 1106 76-77 0.5930728133173773 1106 78-79 0.5534550232578184 1106 80-81 0.5174899920493843 1106 82-83 0.5293778760912318 1106 84-85 0.48071279533362343 1106 86-87 0.5770112684374773 1106 88-89 0.6127756958162678 1106 90-91 0.5634476621646733 1106 92-93 0.4931585573613617 1106 94-95 0.6009852100630475 1106 96-97 0.504325399693716 1106 98-99 0.41988535034874275 1106 100 0.4200224409021125 1107 1 0.32075494755852674 1107 2 0.2978601558124261 1107 3 0.30949893804950435 1107 4 0.2625451792218527 1107 5 0.2823718091359382 1107 6 0.25137172862153534 1107 7 0.19340641765113276 1107 8 0.23548618691447842 1107 9 0.21099129128500493 1107 10-11 0.2976561611481401 1107 12-13 0.36728905171003134 1107 14-15 0.3974232589562874 1107 16-17 0.3878853820821462 1107 18-19 0.40306689418649455 1107 20-21 0.44672309860185777 1107 22-23 0.4764856227663756 1107 24-25 0.48301090080313713 1107 26-27 0.5127486363373492 1107 28-29 0.524102939599608 1107 30-31 0.46359426036583073 1107 32-33 0.49844202178299923 1107 34-35 0.6151084799704236 1107 36-37 0.6975334401830438 1107 38-39 0.6084030507849931 1107 40-41 0.6305212440665002 1107 42-43 0.6531718559882833 1107 44-45 0.6922389069702817 1107 46-47 0.6208283029632753 1107 48-49 0.6529257403501489 1107 50-51 0.574485503167729 1107 52-53 0.6722143533977984 1107 54-55 0.6557071699932422 1107 56-57 0.6399587140170127 1107 58-59 0.6517133772834214 1107 60-61 0.6622390893641139 1107 62-63 0.6897658019804211 1107 64-65 0.7100813198468998 1107 66-67 0.7408716147717271 1107 68-69 0.7184991471675417 1107 70-71 0.7077739967015333 1107 72-73 0.7265608996837472 1107 74-75 0.6506035110410977 1107 76-77 0.6919903131304537 1107 78-79 0.6221061417266114 1107 80-81 0.6649290468510358 1107 82-83 0.6397860113400569 1107 84-85 0.6029204641708006 1107 86-87 0.5912202043191961 1107 88-89 0.6458977078006995 1107 90-91 0.6082839182854336 1107 92-93 0.6202131199283443 1107 94-95 0.6400697855497199 1107 96-97 0.5683198855179938 1107 98-99 0.5697882878533029 1107 100 0.6714554487574453 1108 1 0.26856377931037656 1108 2 0.2938184042313132 1108 3 0.26781682470274504 1108 4 0.22102013814011912 1108 5 0.22612535442817716 1108 6 0.1946165316680748 1108 7 0.21995386369400194 1108 8 0.21757328277485044 1108 9 0.26965537982801635 1108 10-11 0.22371608425108747 1108 12-13 0.22314166513942268 1108 14-15 0.31852508536632484 1108 16-17 0.34813650618765024 1108 18-19 0.27762217191610716 1108 20-21 0.31545198334547564 1108 22-23 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scores pass #Quality Count 0 7.0 1 37.0 2 56.0 3 55.0 4 74.0 5 70.0 6 118.0 7 718.0 8 1667.0 9 2413.0 10 3855.0 11 5994.0 12 8627.0 13 10090.0 14 10299.0 15 10774.0 16 12272.0 17 15430.0 18 19922.0 19 24113.0 20 28897.0 21 34879.0 22 40779.0 23 42358.0 24 40904.0 25 39655.0 26 41784.0 27 47029.0 28 55687.0 29 66834.0 30 83077.0 31 105644.0 32 139399.0 33 188864.0 34 272214.0 35 401689.0 36 631052.0 37 1065221.0 38 1230557.0 39 338204.0 40 702.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.18515519821128 18.609526768548076 12.205966549560147 23.9993514836805 2 15.302400248724243 20.802095820615612 41.52794012282752 22.36756380783262 3 17.695340960106627 27.848219005428287 30.59508828788779 23.861351746577295 4 11.412937610469468 16.869257928008068 39.63476209502495 32.08304236649751 5 11.768909481892415 37.95304314539186 35.73143258777747 14.546614784938258 6 28.500283258478092 37.78678054088928 20.195403546256756 13.517532654375872 7 25.956119844041847 32.9069707239947 24.005667222901476 17.13124220906198 8 25.396712238857326 35.45058560955327 21.830563529715384 17.322138621874014 9 27.00667774114165 15.099692179511637 21.053543400538217 36.8400866788085 10-11 23.818332524362468 26.20608595004294 30.36362866992744 19.611952855667152 12-13 24.467739584395392 24.43424492442394 29.86277942750816 21.235236063672513 14-15 21.928688069227775 25.680095968680135 27.751484374867747 24.639731587224347 16-17 21.175647801908287 28.250698870536002 28.441581432805073 22.132071894750634 18-19 20.905448607840878 27.540432250526308 30.24372567985867 21.31039346177415 20-21 21.943953688872053 27.012434753739615 30.03999666213187 21.003614895256465 22-23 21.47839733102313 27.00093086703701 29.840085289970165 21.6805865119697 24-25 21.05758995474257 27.065466440296536 30.103838168592638 21.773105436368255 26-27 21.053530855474687 27.17705112961945 29.95245222096908 21.81696579393679 28-29 21.08947871799274 27.32060976566748 30.066837767740452 21.52307374859933 30-31 21.724095353792844 27.134626491147714 30.02583992193257 21.11543823312688 32-33 21.244749282502767 27.245765593770848 30.049354727983385 21.460130395743004 34-35 21.442787189307815 27.50184372427723 29.86340407963455 21.191965006780407 36-37 21.450276360004402 27.433045994609724 29.70701009892797 21.409667546457907 38-39 21.26797635671138 27.795661347600536 29.58925168188925 21.347110613798836 40-41 21.47755281615629 27.346504796272324 29.854127636301676 21.32181475126971 42-43 21.284923422824086 27.44800575872473 29.501612968762824 21.76545784968836 44-45 21.171843869545302 27.7644628062135 29.53533797879495 21.528355345446247 46-47 21.114912400721515 27.456327822791128 29.35343090950335 22.075328866984005 48-49 21.09369002238318 27.589721162674707 29.422216433669107 21.89437238127301 50-51 21.248020274302906 27.869718565799378 29.279368893418717 21.602892266478996 52-53 21.283393033203858 27.974452153153646 28.952716129181947 21.789438684460553 54-55 21.18535066948446 27.8817885767901 29.209723731729813 21.723137021995626 56-57 21.316379552937846 27.945955343971868 28.894895443920465 21.842769659169825 58-59 21.24955772763471 27.985262533772282 29.09638813972152 21.668791598871486 60-61 21.53802720404227 28.194184560874497 28.96626033545478 21.301527899628457 62-63 21.379807689913306 28.061933270680907 29.109523717032147 21.44873532237364 64-65 21.28308279269038 27.761904975309438 29.048881691934845 21.90613054006534 66-67 21.182373929140812 27.838027039972435 28.908761751693113 22.07083727919364 68-69 21.07839666448278 27.959115854511683 29.10618786124836 21.856299619757174 70-71 21.149868392169424 27.728367572882295 29.179051587002796 21.942712447945485 72-73 21.082384895480036 28.099190015465886 28.771028310567402 22.047396778486675 74-75 21.22827548428863 28.036870673771226 28.691160798765004 22.043693043175143 76-77 21.05887321449928 27.876580438437447 28.961077849379873 22.103468497683394 78-79 21.04460583123346 27.807363733060047 28.952287427715994 22.195743007990497 80-81 21.108320304016186 28.07672540904554 28.597762001303114 22.21719228563516 82-83 21.350367889006865 28.078767112034203 28.63950023933124 21.93136475962769 84-85 21.28876975953777 28.20804836090484 28.440092359545815 22.063089520011566 86-87 21.056722232805395 28.130309185874296 28.713780031302072 22.09918855001824 88-89 21.021476386430567 27.87659355185625 28.844527093804533 22.25740296790865 90-91 21.464026202017973 27.91229358879929 28.699965462859428 21.92371474632331 92-93 21.274644507734244 27.96218824436927 28.673227547813656 22.089939700082827 94-95 21.14389499914759 27.815488908327 28.698737956735865 22.341878135789546 96-97 21.180933141368026 27.798588180751022 28.69190594204577 22.32857273583518 98-99 21.175136611777 27.67660950067359 28.65790809846424 22.49034578908518 100 21.198471165843607 27.731532164637308 28.67890658240072 22.39109008711837 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2240.0 1 1592.5 2 2658.0 3 3949.0 4 2543.5 5 1835.0 6 2620.0 7 4768.0 8 7341.0 9 8246.5 10 6998.0 11 5618.5 12 5593.0 13 5903.0 14 6311.5 15 6703.5 16 6997.0 17 7524.0 18 8240.0 19 8947.0 20 10117.5 21 11917.0 22 14256.5 23 17590.5 24 22056.0 25 27378.5 26 34063.0 27 42034.0 28 50612.0 29 60541.0 30 71612.0 31 82323.5 32 94329.0 33 108038.5 34 121927.0 35 132615.5 36 145114.5 37 158083.0 38 165659.0 39 171927.5 40 174138.0 41 173897.5 42 173626.5 43 174492.0 44 176596.0 45 179333.5 46 181037.0 47 180445.5 48 182227.5 49 184057.5 50 184113.5 51 176523.5 52 162755.5 53 151995.5 54 142126.5 55 129913.0 56 116703.0 57 103663.0 58 89866.0 59 75364.5 60 60003.5 61 47083.0 62 37377.0 63 28614.5 64 21146.0 65 15742.5 66 11760.0 67 8985.0 68 6992.5 69 5343.0 70 4163.5 71 3223.0 72 2516.0 73 2011.5 74 1607.0 75 1246.0 76 928.5 77 632.0 78 442.5 79 336.0 80 250.5 81 194.5 82 160.5 83 138.0 84 112.0 85 82.0 86 69.0 87 58.0 88 43.0 89 33.0 90 32.0 91 28.0 92 16.0 93 13.0 94 15.0 95 14.5 96 8.5 97 4.0 98 5.0 99 5.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14928255960748862 2 0.08791283188836364 3 0.02560722577767512 4 0.027917053297278782 5 0.0221623968044731 6 0.002090792151365387 7 0.00318596899255678 8 0.0048586027136490895 9 0.0052170242253117265 10-11 0.004131803537222074 12-13 0.00645158720992748 14-15 0.01589997650347868 16-17 0.006889657946404036 18-19 0.01431694816030203 20-21 0.016945372579161372 22-23 0.039207330914651874 24-25 0.014834668121592507 26-27 0.008751458576429404 28-29 0.02401424128139673 30-31 0.01433686046650551 32-33 0.018329277860303226 34-35 0.018578181687846723 36-37 0.01876734859677978 38-39 0.01605927495310652 40-41 0.021694457608691323 42-43 0.029151616281894537 44-45 0.031152803055344264 46-47 0.018259584788591045 48-49 0.012256024468241863 50-51 0.009776942345908618 52-53 0.018548313228541502 54-55 0.021246430719113026 56-57 0.016706424904719613 58-59 0.021415685321842605 60-61 0.021405729168740867 62-63 0.04505159278537322 64-65 0.02742920179529353 66-67 0.018618006300253682 68-69 0.022889195980900116 70-71 0.035881975778670736 72-73 0.03216833067172174 74-75 0.03524478198015938 76-77 0.019603665457325937 78-79 0.013560280524569795 80-81 0.019344805476680698 82-83 0.014665413518862927 84-85 0.015412125001493422 86-87 0.018846997821593704 88-89 0.01607918725931 90-91 0.02664266570025607 92-93 0.02390472359727759 94-95 0.019036164730526762 96-97 0.012475059836480142 98-99 0.015242870398763845 100 0.0343288158947993 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5022020.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.30029354368149 #Duplication Level Percentage of deduplicated Percentage of total 1 79.73343661195527 53.6608368923111 2 13.778469775847253 18.545901209945264 3 3.478435454279969 7.02299181437373 4 1.2033056842105456 3.239313030806009 5 0.532373977243488 1.7914462471751977 6 0.3224562510457853 1.3020840210225841 7 0.20278129190749952 0.9553068329379176 8 0.12782646836448203 0.6882207074865405 9 0.10325412250100899 0.6254129478521837 >10 0.46236921907733464 5.393648217470674 >50 0.03253964629180708 1.5315628856475598 >100 0.020648735269145447 2.694463851903318 >500 0.0015125130682910166 0.6982225563369528 >1k 4.426867035603679E-4 0.5302151129184546 >5k 3.689055863002911E-5 0.16470533148065825 >10k+ 1.1067167589008733E-4 1.1556683403318782 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19210 0.3825154021688484 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16062 0.31983146224029374 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11276 0.22453116475043908 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6634 0.1320982393538855 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.9912306203479874E-5 2 0.0 0.0 0.0 0.0 1.9912306203479874E-5 3 0.0 0.0 0.0 0.0 1.9912306203479874E-5 4 0.0 1.9912306203479874E-5 0.0 0.0 1.9912306203479874E-5 5 0.0 3.982461240695975E-5 0.0 0.0 1.9912306203479874E-5 6 3.982461240695975E-5 3.982461240695975E-5 0.0 0.0 1.9912306203479874E-5 7 3.982461240695975E-5 3.982461240695975E-5 0.0 0.0 1.9912306203479874E-5 8 3.982461240695975E-5 3.982461240695975E-5 0.0 0.0 1.9912306203479874E-5 9 3.982461240695975E-5 3.982461240695975E-5 0.0 0.0 1.9912306203479874E-5 10-11 3.982461240695975E-5 3.982461240695975E-5 0.0 0.0 1.9912306203479874E-5 12-13 3.982461240695975E-5 3.982461240695975E-5 0.0 0.0 1.9912306203479874E-5 14-15 3.982461240695975E-5 4.9780765508699684E-5 0.0 0.0 3.982461240695975E-5 16-17 3.982461240695975E-5 5.9736918610439626E-5 0.0 0.0 5.9736918610439626E-5 18-19 3.982461240695975E-5 5.9736918610439626E-5 0.0 1.9912306203479874E-5 8.960537791565944E-5 20-21 3.982461240695975E-5 6.969307171217956E-5 0.0 5.9736918610439626E-5 1.1947383722087925E-4 22-23 3.982461240695975E-5 7.96492248139195E-5 0.0 1.891669089330588E-4 1.393861434243591E-4 24-25 3.982461240695975E-5 7.96492248139195E-5 0.0 3.0864074615393806E-4 1.59298449627839E-4 26-27 3.982461240695975E-5 7.96492248139195E-5 0.0 4.8785150198525694E-4 1.59298449627839E-4 28-29 3.982461240695975E-5 7.96492248139195E-5 0.0 0.0013341245156331515 1.59298449627839E-4 30-31 3.982461240695975E-5 7.96492248139195E-5 0.0 0.004689348110919511 1.6925460272957895E-4 32-33 3.982461240695975E-5 7.96492248139195E-5 0.0 0.012106682171715764 1.7921075583131888E-4 34-35 3.982461240695975E-5 7.96492248139195E-5 0.0 0.02343678440149581 1.9912306203479874E-4 36-37 3.982461240695975E-5 7.96492248139195E-5 0.0 0.04278158987817651 1.9912306203479874E-4 38-39 3.982461240695975E-5 8.960537791565944E-5 0.0 0.07619443968761574 1.9912306203479874E-4 40-41 3.982461240695975E-5 9.956153101739937E-5 0.0 0.11940414414916706 2.0907921513653867E-4 42-43 3.982461240695975E-5 9.956153101739937E-5 0.0 0.1599754680387573 2.1903536823827862E-4 44-45 3.982461240695975E-5 1.1947383722087925E-4 0.0 0.20615210612462714 2.389476744417585E-4 46-47 5.9736918610439626E-5 1.1947383722087925E-4 0.0 0.26171739658543774 2.5885998064523836E-4 48-49 5.9736918610439626E-5 1.1947383722087925E-4 0.0 0.32461041572912896 2.5885998064523836E-4 50-51 5.9736918610439626E-5 1.1947383722087925E-4 0.0 0.3969717364725748 2.5885998064523836E-4 52-53 5.9736918610439626E-5 1.1947383722087925E-4 0.0 0.47165284088872605 2.688161337469783E-4 54-55 5.9736918610439626E-5 1.1947383722087925E-4 0.0 0.561049139589249 2.787722868487182E-4 56-57 6.969307171217956E-5 1.1947383722087925E-4 0.0 0.6594258883875412 2.787722868487182E-4 58-59 7.96492248139195E-5 1.1947383722087925E-4 0.0 0.7725775683888156 2.787722868487182E-4 60-61 7.96492248139195E-5 1.2942999032261918E-4 0.0 0.8958247876352543 2.787722868487182E-4 62-63 7.96492248139195E-5 1.393861434243591E-4 0.0 1.0261010509715214 2.9868459305219813E-4 64-65 7.96492248139195E-5 1.393861434243591E-4 0.0 1.1618830669730507 3.18596899255678E-4 66-67 7.96492248139195E-5 1.393861434243591E-4 0.0 1.3146602363192499 3.18596899255678E-4 68-69 7.96492248139195E-5 1.393861434243591E-4 0.0 1.4990481917634737 3.285530523574179E-4 70-71 7.96492248139195E-5 1.393861434243591E-4 0.0 1.7084659161054714 3.4846535856089783E-4 72-73 7.96492248139195E-5 1.393861434243591E-4 0.0 1.9125272300787333 3.5842151166263776E-4 74-75 7.96492248139195E-5 1.393861434243591E-4 0.0 2.1186195992847496 3.683776647643777E-4 76-77 7.96492248139195E-5 1.393861434243591E-4 0.0 2.342264666409134 3.783338178661176E-4 78-79 7.96492248139195E-5 1.393861434243591E-4 0.0 2.6026578946320402 3.783338178661176E-4 80-81 7.96492248139195E-5 1.393861434243591E-4 0.0 2.8827643059963917 3.8828997096785754E-4 82-83 7.96492248139195E-5 1.393861434243591E-4 0.0 3.181837189019558 4.181584302730774E-4 84-85 7.96492248139195E-5 1.4934229652609904E-4 0.0 3.4843150764035187 4.181584302730774E-4 86-87 9.956153101739937E-5 1.59298449627839E-4 0.0 3.8034296956204874 4.181584302730774E-4 88 9.956153101739937E-5 1.59298449627839E-4 0.0 4.051457381691033 4.181584302730774E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 6985 0.0 57.076286 2 GTACCTG 7870 0.0 51.43167 1 ACCTGGG 8030 0.0 48.225147 3 GGTATCA 10340 0.0 41.012016 1 TATAACG 645 0.0 40.81827 2 TAACGCA 705 0.0 39.995888 4 GTATCAA 17005 0.0 35.09535 1 ATAACGC 885 0.0 31.861767 3 CCTGGGG 5955 0.0 31.803616 4 TCAACGC 20085 0.0 28.826513 4 ATCAACG 20195 0.0 28.693338 3 CAACGCA 20275 0.0 28.6242 5 CATGGGG 9430 0.0 27.80838 4 TATCAAC 20970 0.0 27.710623 2 AACGCAG 21075 0.0 27.644442 6 GTACATG 20095 0.0 27.216043 1 TACATGG 20210 0.0 26.635782 2 ACATGGG 19720 0.0 26.024212 3 ATGGGGG 6230 0.0 25.117907 5 ACGCAGA 23530 0.0 24.681274 7 >>END_MODULE