FastQCFastQC Report
Mon 6 Jun 2016
ERR1211467_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211467_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5022020
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216270.43064344626265927No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185420.36921398162492386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147060.292830375028375No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63810.12706042588440508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG68950.054.900092
GTACCTG75750.050.530451
ACCTGGG77550.047.9333653
TAACGCA6650.040.2806474
GGTATCA121350.039.4862481
TATAACG6700.035.7937162
GTATCAA190900.033.6970251
CCTGGGG59600.032.328154
ATAACGC9300.027.2868923
TCAACGC237050.026.7036674
ATCAACG238050.026.5520083
CAACGCA241100.026.2158555
TATCAAC248800.025.6094552
AACGCAG247550.025.5897396
GTACATG207500.024.9052581
CATGGGG95200.024.23754
TACATGG209950.023.9875012
ACATGGG204000.023.4279373
ACGCAGA276300.022.8075287
ATGGGGG59450.022.7656255
Produced by FastQC (version 0.11.5)