FastQCFastQC Report
Mon 6 Jun 2016
ERR1211466_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211466_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794572
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28840.160706842634344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21780.12136598587295466No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18140.10108259796764911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26250.043.6032451
GTATCAA47200.035.42651
GTACATG86450.032.3095971
TACATGG91150.030.3360652
CAACGCA52950.030.2604775
ATCAACG52950.030.0871893
TCAACGC53450.029.979914
ACATGGG90350.029.3358613
AACGCAG56950.028.4571676
TATCAAC60550.026.173052
CATGGGG34350.025.8558444
CATGGGA48150.025.1794994
ACGCAGA64300.024.8390087
CGCAGAG64500.024.4720348
TACCTGG13000.023.5130272
CTACACT8050.021.0150434
TACGAGT1150.003963408520.4312924
GCAGAGT75000.020.4218589
GTACCTG15550.019.9919591
TATACCG1200.00507267719.5783525
Produced by FastQC (version 0.11.5)