FastQCFastQC Report
Mon 6 Jun 2016
ERR1211466_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211466_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1794572
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37410.20846196196084638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32420.1806558889807709No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23880.13306794043370787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31000.042.016321
GTATCAA54250.034.930531
GTACATG93250.029.196421
ATCAACG64000.027.827923
CAACGCA64800.027.62945
TACATGG98500.027.4493162
TCAACGC65600.027.3640924
ACATGGG94300.027.058873
AACGCAG67200.026.9915266
CATGGGG36150.025.4782314
TATCAAC73450.024.5192342
ACGCAGA76500.023.5259277
CGCAGAG77200.023.1300058
CATGGGA53700.022.402024
ATGGGGG19100.020.9125545
GACATAT6550.020.8188441
TGCGTAT4650.020.21882494
GCAGAGT88600.019.7826529
TATAGGG7150.019.7294582
AGAGTAC83250.019.19128610-11
Produced by FastQC (version 0.11.5)