##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211466_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1794572 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.161067931517934 33.0 31.0 34.0 30.0 34.0 2 32.400353399027736 34.0 31.0 34.0 31.0 34.0 3 32.420467944445804 34.0 31.0 34.0 31.0 34.0 4 36.03212576592079 37.0 35.0 37.0 35.0 37.0 5 35.96534271124257 37.0 35.0 37.0 35.0 37.0 6 35.985501278299225 37.0 35.0 37.0 35.0 37.0 7 35.91456347251601 37.0 35.0 37.0 35.0 37.0 8 35.91587297695495 37.0 35.0 37.0 35.0 37.0 9 37.58679785486456 39.0 37.0 39.0 35.0 39.0 10-11 37.550391681136226 39.0 37.0 39.0 35.0 39.0 12-13 37.479676212489665 39.0 37.0 39.0 35.0 39.0 14-15 38.728846766805674 40.0 38.0 41.0 35.0 41.0 16-17 38.66354261628957 40.0 38.0 41.0 34.0 41.0 18-19 38.657107934370984 40.0 38.0 41.0 34.0 41.0 20-21 38.6046360915026 40.0 38.0 41.0 34.0 41.0 22-23 38.497210755545055 40.0 38.0 41.0 34.0 41.0 24-25 38.396889063241815 40.0 38.0 41.0 34.0 41.0 26-27 38.23015181335717 40.0 38.0 41.0 34.0 41.0 28-29 38.283144671821475 40.0 38.0 41.0 34.0 41.0 30-31 38.23924618237663 40.0 38.0 41.0 34.0 41.0 32-33 38.09743827497587 40.0 38.0 41.0 33.5 41.0 34-35 38.01540005081992 40.0 37.0 41.0 33.0 41.0 36-37 37.96230103891067 40.0 37.0 41.0 33.0 41.0 38-39 37.893355908818364 40.0 37.0 41.0 33.0 41.0 40-41 37.758183566889485 40.0 37.0 41.0 33.0 41.0 42-43 37.632329045588584 40.0 37.0 41.0 32.5 41.0 44-45 37.47470093147558 40.0 37.0 41.0 32.0 41.0 46-47 37.254618650017946 40.0 36.5 41.0 31.0 41.0 48-49 37.05998700525808 39.5 36.0 41.0 31.0 41.0 50-51 36.893686628343694 39.0 36.0 41.0 30.0 41.0 52-53 36.72198607801749 39.0 36.0 41.0 30.0 41.0 54-55 36.54204372964696 39.0 35.0 40.0 30.0 41.0 56-57 36.35220542836955 39.0 35.0 40.0 29.0 41.0 58-59 36.11164639813839 38.5 35.0 40.0 28.5 41.0 60-61 35.807812949271465 38.0 34.5 40.0 28.0 41.0 62-63 35.499967401698015 38.0 34.0 40.0 28.0 41.0 64-65 35.20010230851702 37.5 34.0 40.0 27.5 41.0 66-67 34.865892814554115 37.0 34.0 39.5 26.5 41.0 68-69 34.46586651301814 36.0 33.0 39.0 26.0 40.5 70-71 34.070536595912564 36.0 33.0 39.0 26.0 40.0 72-73 33.65994872314958 35.5 32.0 38.0 26.0 40.0 74-75 33.22313064062072 35.0 32.0 37.5 25.0 39.5 76-77 31.668773947214156 34.0 30.0 36.0 23.0 38.5 78-79 32.43781274866653 34.5 31.0 36.0 25.0 39.0 80-81 32.37268969982814 35.0 31.0 36.0 25.0 37.5 82-83 32.06410971529702 34.5 31.0 36.0 24.0 37.0 84-85 31.745951402339944 34.0 31.0 35.0 24.0 37.0 86-87 31.4586444567284 34.0 31.0 35.0 23.0 36.0 88-89 31.17110597958733 34.0 30.5 35.0 21.5 36.0 90-91 30.88627594769115 34.0 30.0 35.0 20.0 36.0 92-93 30.67043534614382 34.0 30.0 35.0 20.0 35.0 94-95 30.450342755821445 34.0 29.0 35.0 20.0 35.0 96-97 30.253482167335722 34.0 29.0 35.0 19.0 35.0 98-99 30.095173946768366 34.0 29.0 35.0 19.0 35.0 100 28.037802885590548 32.0 25.0 34.0 11.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1806351078670403 1101 2 0.09381421859514205 1101 3 0.17136947243867695 1101 4 0.12023487708503922 1101 5 0.12622555273951974 1101 6 0.19220706532286158 1101 7 0.16647859643445884 1101 8 0.19107848693656138 1101 9 0.22098690513378472 1101 10-11 0.22453176597553437 1101 12-13 0.13523926665422437 1101 14-15 0.17799377697333796 1101 16-17 0.1925245989793538 1101 18-19 0.13550851253562968 1101 20-21 0.2716119517867668 1101 22-23 0.21725273608434748 1101 24-25 0.1837107049106308 1101 26-27 0.13870979142568274 1101 28-29 0.1219595230994841 1101 30-31 0.2880708269576431 1101 32-33 0.24500580296505348 1101 34-35 0.33945601863201347 1101 36-37 0.29534110624921794 1101 38-39 0.3292464441363947 1101 40-41 0.2770223498870479 1101 42-43 0.23388952171027455 1101 44-45 0.2366286890956033 1101 46-47 0.34692679707735863 1101 48-49 0.31657076986648036 1101 50-51 0.3440429010209911 1101 52-53 0.3460399308289013 1101 54-55 0.2566890555511705 1101 56-57 0.40062826698363097 1101 58-59 0.45636407247831556 1101 60-61 0.3906886544763424 1101 62-63 0.31651286400144585 1101 64-65 0.2289338049046492 1101 66-67 -0.03123035299034882 1101 68-69 0.16477415706597753 1101 70-71 0.1652273495514862 1101 72-73 0.22331235868324484 1101 74-75 0.05023973711232799 1101 76-77 0.05828369819596446 1101 78-79 0.2862348755692494 1101 80-81 0.15871622118658024 1101 82-83 0.19480614398852225 1101 84-85 0.06066473710426834 1101 86-87 0.17390563239546353 1101 88-89 0.12453886220940191 1101 90-91 0.02630930464385628 1101 92-93 0.06499872024404496 1101 94-95 0.09406044354304655 1101 96-97 0.07256561244690474 1101 98-99 0.028036147364808528 1101 100 0.19120773012743086 1102 1 0.31283973317812297 1102 2 0.1261002794497017 1102 3 0.24287728284762267 1102 4 0.16385129859085623 1102 5 0.17224005168313283 1102 6 0.13551539851382444 1102 7 0.14962428633018732 1102 8 0.12185542757972456 1102 9 0.18793432510770458 1102 10-11 0.20448894113028615 1102 12-13 0.2725270644207214 1102 14-15 0.24907960320241784 1102 16-17 0.26986558611481826 1102 18-19 0.18604780945645416 1102 20-21 0.21446156938139893 1102 22-23 0.27203664715445797 1102 24-25 0.16697513124562846 1102 26-27 0.2703819535678349 1102 28-29 0.2738302626822744 1102 30-31 0.2870232697392012 1102 32-33 0.2956999982622932 1102 34-35 0.36948893453700293 1102 36-37 0.30273693424368275 1102 38-39 0.2547129900734362 1102 40-41 0.34406287973060756 1102 42-43 0.343483824639101 1102 44-45 0.2678492541329902 1102 46-47 0.16413219118553002 1102 48-49 0.27988576596553827 1102 50-51 0.21001508765076693 1102 52-53 0.30974416604664157 1102 54-55 0.31219905276072524 1102 56-57 0.23385453820385038 1102 58-59 0.2260156650272407 1102 60-61 0.2904987331214315 1102 62-63 0.46024261189295856 1102 64-65 0.38668669643592324 1102 66-67 0.4381202697352933 1102 68-69 0.3950411289735598 1102 70-71 0.3964026409825294 1102 72-73 0.4309482222132175 1102 74-75 0.27954030260529095 1102 76-77 0.4186049414832276 1102 78-79 0.37786011261501073 1102 80-81 0.29841035384109915 1102 82-83 0.33090629262262183 1102 84-85 0.11020915364422379 1102 86-87 0.1277479094015952 1102 88-89 0.23106082417957197 1102 90-91 0.21654785953311873 1102 92-93 0.2647441629857461 1102 94-95 0.1598304650124156 1102 96-97 0.1697327375444253 1102 98-99 0.10339733962672071 1102 100 0.23022218920749182 1103 1 0.2779240509518601 1103 2 0.2625491532291804 1103 3 0.3112753227633789 1103 4 0.14071526729573947 1103 5 0.18384118385428394 1103 6 0.2141961190051873 1103 7 0.23415576513819758 1103 8 0.19788165172684558 1103 9 0.2878360104282862 1103 10-11 0.22857991842995062 1103 12-13 0.3128999875231244 1103 14-15 0.41387699734596595 1103 16-17 0.4937397536740775 1103 18-19 0.4344238061861958 1103 20-21 0.30096141156046485 1103 22-23 0.36841400994079976 1103 24-25 0.3204645261963961 1103 26-27 0.37468943014047795 1103 28-29 0.3948985810355623 1103 30-31 0.4760298775205527 1103 32-33 0.4832101531337898 1103 34-35 0.4415644609086158 1103 36-37 0.3090169137528349 1103 38-39 0.33281675863033655 1103 40-41 0.348194683494512 1103 42-43 0.4044606585959585 1103 44-45 0.3661171047153715 1103 46-47 0.4756608809613212 1103 48-49 0.5241977443156145 1103 50-51 0.5570423622126199 1103 52-53 0.41490956755861674 1103 54-55 0.6627506498568181 1103 56-57 0.6118907800993654 1103 58-59 0.7022918154383504 1103 60-61 0.6055597658395229 1103 62-63 0.6733585662956401 1103 64-65 0.5820149381037609 1103 66-67 0.6513391958285268 1103 68-69 0.7128826560592572 1103 70-71 0.806680147101325 1103 72-73 0.7759822960113212 1103 74-75 0.8163174956694519 1103 76-77 0.7968949904853311 1103 78-79 0.8785214081955104 1103 80-81 0.8317488616312403 1103 82-83 0.8019396833924262 1103 84-85 0.6682349948815443 1103 86-87 0.7438883338105491 1103 88-89 0.743625440209005 1103 90-91 0.7636444718162458 1103 92-93 0.7084649596451165 1103 94-95 0.8942532802273888 1103 96-97 0.7480158010298403 1103 98-99 0.721686715949442 1103 100 0.8864690521930498 1104 1 0.12801581302843346 1104 2 0.10511696880235633 1104 3 0.11401459067873532 1104 4 0.03355592210991887 1104 5 0.07829576366554392 1104 6 0.06795401394236933 1104 7 0.08942970552411822 1104 8 0.08849255605230866 1104 9 0.12984645635119563 1104 10-11 0.13578738772704213 1104 12-13 0.10104255996078138 1104 14-15 0.2052705302266986 1104 16-17 0.16257109905565414 1104 18-19 0.2484763185638812 1104 20-21 0.017257132864770597 1104 22-23 0.26446946414268524 1104 24-25 0.15970810760907028 1104 26-27 0.12773035738607064 1104 28-29 0.1768206362885678 1104 30-31 0.15009995383940833 1104 32-33 0.08245512117338905 1104 34-35 0.15404070472657594 1104 36-37 0.04792987980222563 1104 38-39 0.25443712736434776 1104 40-41 0.34926728021174114 1104 42-43 0.32582083086396807 1104 44-45 0.15762374285196046 1104 46-47 0.07310418559238485 1104 48-49 0.07722195047169578 1104 50-51 0.16939815980858697 1104 52-53 0.07372348494283898 1104 54-55 0.14567311566015206 1104 56-57 0.24570626083748692 1104 58-59 0.2774295944970291 1104 60-61 0.19052737042789403 1104 62-63 0.28829892774306387 1104 64-65 0.4074967909636058 1104 66-67 0.26441767075640144 1104 68-69 0.184473253097309 1104 70-71 -0.005809147323070363 1104 72-73 0.1983808071546349 1104 74-75 0.18236892593435527 1104 76-77 0.18112618741626463 1104 78-79 0.3058353758789423 1104 80-81 0.40859368720172995 1104 82-83 0.1875681108759224 1104 84-85 0.19131947852613962 1104 86-87 0.28808665952649193 1104 88-89 0.34196950857242925 1104 90-91 0.2039855276646847 1104 92-93 0.22806452353735196 1104 94-95 0.21883355369196877 1104 96-97 0.21605201102497062 1104 98-99 0.31735309894667907 1104 100 0.7664032010080284 1105 1 0.11452403759359697 1105 2 0.08299509676822936 1105 3 0.044227945436816185 1105 4 0.04609920620392671 1105 5 -0.020871502048287027 1105 6 -0.007449285750354306 1105 7 0.020325504206681444 1105 8 0.03245786065016887 1105 9 -0.011166015575810206 1105 10-11 -0.08103553040788114 1105 12-13 0.031245343298344608 1105 14-15 -4.2831701863121907E-4 1105 16-17 0.041423207231829906 1105 18-19 0.07999665327854899 1105 20-21 2.450743977675529E-4 1105 22-23 0.08062012802789553 1105 24-25 0.16796012909959046 1105 26-27 0.12742481396806937 1105 28-29 0.10335102559874798 1105 30-31 0.07469210256285663 1105 32-33 0.1383870388760542 1105 34-35 0.2825609877587425 1105 36-37 0.3061446076703689 1105 38-39 0.2509238919458596 1105 40-41 0.2274735039593807 1105 42-43 0.1750927830059723 1105 44-45 0.2437197407470748 1105 46-47 0.39397117128102366 1105 48-49 0.34412271537269845 1105 50-51 0.4642753081561537 1105 52-53 0.4103975339848347 1105 54-55 0.24517896048109122 1105 56-57 0.3653010347970209 1105 58-59 0.5029870020088438 1105 60-61 0.4200565487231316 1105 62-63 0.5263950677650513 1105 64-65 0.5084701545077124 1105 66-67 0.5485439780887447 1105 68-69 0.5143943668082045 1105 70-71 0.5692341367709233 1105 72-73 0.4865976669259169 1105 74-75 0.6887201472857996 1105 76-77 0.42422906965533613 1105 78-79 0.517951956329803 1105 80-81 0.5074696515502026 1105 82-83 0.4865300854763177 1105 84-85 0.5089958039473501 1105 86-87 0.44251382361373004 1105 88-89 0.41966988984833264 1105 90-91 0.6526303009194621 1105 92-93 0.699849989478583 1105 94-95 0.5536636239801709 1105 96-97 0.47188229626538813 1105 98-99 0.5887900310264733 1105 100 0.7794771116818744 1106 1 0.15895650684034734 1106 2 0.22116586386740522 1106 3 0.2518497881973971 1106 4 0.0695918525743906 1106 5 0.08504873143296265 1106 6 0.07360973520116687 1106 7 0.0846311455779869 1106 8 0.11092251548362952 1106 9 0.10102422857779914 1106 10-11 0.06465357395655502 1106 12-13 0.1704026014383544 1106 14-15 0.24952809248163987 1106 16-17 0.22351915548024692 1106 18-19 0.2213691279551 1106 20-21 0.2195567774815146 1106 22-23 0.17812125143406377 1106 24-25 0.14823703701772928 1106 26-27 0.13145825168456327 1106 28-29 0.20043087169209883 1106 30-31 0.15160534518113877 1106 32-33 0.29071330879310864 1106 34-35 0.2604298862589971 1106 36-37 0.23545456713413415 1106 38-39 0.34782469518126646 1106 40-41 0.33977462596129016 1106 42-43 0.32807934660011995 1106 44-45 0.39273488800690615 1106 46-47 0.37100161090730666 1106 48-49 0.45104874843828924 1106 50-51 0.38019596291471913 1106 52-53 0.48107060640708 1106 54-55 0.40453997641473904 1106 56-57 0.5673415382702203 1106 58-59 0.5995993473064516 1106 60-61 0.6182081360024299 1106 62-63 0.6017993184531392 1106 64-65 0.6488195689322467 1106 66-67 0.7158928799592132 1106 68-69 0.7468949753198828 1106 70-71 0.6019983946675396 1106 72-73 0.6081689376855337 1106 74-75 0.5479705194141005 1106 76-77 0.46767358509280754 1106 78-79 0.519359432129761 1106 80-81 0.4275749942827005 1106 82-83 0.30620320938802337 1106 84-85 0.3839978518231746 1106 86-87 0.312239373146582 1106 88-89 0.3859299653973949 1106 90-91 0.4915832980734969 1106 92-93 0.5801498921167116 1106 94-95 0.6756029272811119 1106 96-97 0.6591169500690555 1106 98-99 0.6491119688084979 1106 100 0.9015831705858055 1107 1 0.20334752474360585 1107 2 0.1596177669381902 1107 3 0.22136003072022703 1107 4 0.12624997802426918 1107 5 0.09974167774205966 1107 6 0.06784510760432738 1107 7 0.12742241966224555 1107 8 0.1203542054513349 1107 9 0.16307430930034172 1107 10-11 0.12099896544562938 1107 12-13 0.18962588904648925 1107 14-15 0.13819272124395354 1107 16-17 0.1975337937605559 1107 18-19 0.06314558449895458 1107 20-21 0.09987609911243567 1107 22-23 0.19216972159058798 1107 24-25 0.2590454501440078 1107 26-27 0.21425588589515598 1107 28-29 0.3268282541615761 1107 30-31 0.2746807271078282 1107 32-33 0.24168899990186787 1107 34-35 0.3742625505580932 1107 36-37 0.3473943168692628 1107 38-39 0.2700722894607068 1107 40-41 0.2906017198774862 1107 42-43 0.25606506794999717 1107 44-45 0.42454328947849973 1107 46-47 0.29831385814640043 1107 48-49 0.3569470441353175 1107 50-51 0.20100536946386427 1107 52-53 0.28742501639992213 1107 54-55 0.3877074247775667 1107 56-57 0.3520276749152558 1107 58-59 0.32759544687295516 1107 60-61 0.3890661314748627 1107 62-63 0.40556250419934514 1107 64-65 0.4698530506130254 1107 66-67 0.31928836049561937 1107 68-69 0.2608260661814441 1107 70-71 0.460779864389508 1107 72-73 0.37131042385725266 1107 74-75 0.4101895652730505 1107 76-77 0.26870735536571644 1107 78-79 0.6129870406279139 1107 80-81 0.6530386979962657 1107 82-83 0.5773639490940212 1107 84-85 0.3902972073192714 1107 86-87 0.33675929738005195 1107 88-89 0.3087168662010704 1107 90-91 0.21265207679049425 1107 92-93 0.5439583590765231 1107 94-95 0.5575680564732615 1107 96-97 0.6559529814612155 1107 98-99 0.6107639034024253 1107 100 0.8249669575914993 1108 1 0.2533292483617089 1108 2 0.10954764774751169 1108 3 0.22959037524794468 1108 4 0.12382666516526086 1108 5 0.15204079880338384 1108 6 0.1745019313045333 1108 7 0.1859578085510023 1108 8 0.18468627337416876 1108 9 0.20647573907881878 1108 10-11 0.19620169790409392 1108 12-13 0.1815301287689124 1108 14-15 0.2541553689382283 1108 16-17 0.30677240526519967 1108 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2316 80-81 -0.3942941634090431 2316 82-83 -0.4204284233904936 2316 84-85 -0.5309571874752308 2316 86-87 -0.4701779882238668 2316 88-89 -0.415302694905872 2316 90-91 -0.2309019011717588 2316 92-93 -0.25548207716721194 2316 94-95 -0.18997773502849213 2316 96-97 -0.17322918528461884 2316 98-99 -0.4053843033695479 2316 100 -0.763295534509048 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 2.0 11 3.0 12 16.0 13 39.0 14 100.0 15 286.0 16 723.0 17 1426.0 18 2551.0 19 3997.0 20 5699.0 21 8550.0 22 11351.0 23 13446.0 24 14553.0 25 16445.0 26 19920.0 27 24207.0 28 29807.0 29 36619.0 30 44775.0 31 54218.0 32 67772.0 33 86281.0 34 114575.0 35 158640.0 36 239825.0 37 386875.0 38 395619.0 39 56246.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.31366902145051 22.821973549222264 14.173450810614415 22.690906618712813 2 14.680804199996098 22.63230238633607 41.00260129869768 21.68429211497015 3 16.977402767035414 29.2329690463825 30.56354886668346 23.226079319898627 4 11.101687548340648 18.224842245101293 40.791649856566195 29.881820349991866 5 11.597577081464227 40.47561199868489 36.06526499974924 11.861545920101642 6 28.788855646429013 40.29987311645925 18.738587028902366 12.17268420820937 7 26.30510227508286 33.225805373091745 24.005835374674295 16.463256977151097 8 25.408374448470205 37.00473766740928 20.924307655272564 16.662580228847947 9 27.03686450028196 14.756666213448108 20.97073842676694 37.23573085950299 10-11 23.857805649480767 27.52015522364107 29.747148623738696 18.874890503139465 12-13 24.76487429871858 25.252539324139683 29.36190356252076 20.62068281462098 14-15 22.38642417244892 26.321763629433647 26.757048477297097 24.53476372082034 16-17 21.367722831146025 29.31159429375287 27.63182273978656 21.688860135314545 18-19 21.334657719167975 28.599016183790578 29.307386144379414 20.75893995266203 20-21 22.30290314806069 27.900535937933178 29.05722568879936 20.739335225206776 22-23 22.1415189805703 27.927438965948426 28.542933914047474 21.388108139433804 24-25 21.67990103478828 28.350273324528995 28.83830109719877 21.131524543483955 26-27 21.577270085463418 28.25633898724429 28.968181217885743 21.198209709406548 28-29 21.478637686707618 27.787376281692826 29.460747970034834 21.27323806156472 30-31 21.924708155919884 28.03073996245271 28.933312603752388 21.11123927787502 32-33 21.854419961237504 28.094201339858394 28.880066959484385 21.17131173941972 34-35 21.950378866507197 28.267579085510008 29.02060326080142 20.76143878718137 36-37 21.943581179163722 27.910002713782838 28.870191463787716 21.276224643265724 38-39 21.812748670968602 28.303353505633755 28.9180514226487 20.965846400748937 40-41 21.77872674312011 27.961112730284 29.29925231363707 20.96090821295882 42-43 21.79930535528444 27.742599721083288 28.988306953273792 21.46978797035848 44-45 21.682589641967105 28.065748587885466 28.879469838481736 21.372191931665693 46-47 21.842534292703327 28.107296894326172 28.53985487963804 21.51031393333246 48-49 21.64259577735895 28.197352685154332 28.673244086396622 21.4868074510901 50-51 21.797463148444436 28.0538823213401 28.391204110053952 21.75745042016151 52-53 21.929240417520433 28.135958838650787 28.34718771088755 21.587613032941228 54-55 21.82987593140605 28.20630342794206 28.49150321407008 21.472317426581814 56-57 21.767733238749003 28.2771116597572 28.30216016162553 21.652994939868268 58-59 21.634103405559298 28.133214303427067 28.690721184146955 21.541961106866676 60-61 21.92659213515424 28.552258389531964 28.23639620642162 21.284753268892175 62-63 21.777779635465865 28.11165076950085 28.63744151765686 21.473128077376423 64-65 21.587851769295067 27.998523265533574 28.603928670938984 21.809696294232378 66-67 21.659775757038734 28.119035239214597 28.303275815275697 21.917913188470976 68-69 21.59054462953917 28.276330392926972 28.37809332708786 21.755031650445993 70-71 21.68976763431138 27.928575309526032 28.486704347087105 21.894952709075483 72-73 21.680771361284144 28.293872510501895 28.157757470584105 21.867598657629856 74-75 21.617128198183966 28.40252892913376 28.06809306918389 21.912249803498383 76-77 21.476286044735552 28.225124942637976 28.410243722079986 21.88834529054649 78-79 21.583050140936745 28.05867293200819 28.25923890812752 22.099038018927544 80-81 21.41815633546352 28.59670900572183 27.94842966548106 22.036704993333583 82-83 21.82661365381807 28.260134137220348 28.07504617445864 21.83820603450294 84-85 21.469981396167988 28.319513290760394 28.01910989191051 22.191395421161104 86-87 21.271370203957307 28.34607224659586 28.33835281908198 22.044204730364854 88-89 21.602224478853742 28.064259749930052 28.226057257256365 22.107458513959845 90-91 21.654842145569525 28.24781387094635 28.163010623954595 21.93433335952954 92-93 21.65849952368397 28.164129194521447 27.984361003513424 22.193010278281157 94-95 21.332511658968347 27.85080311143666 28.158011213569555 22.658674016025437 96-97 21.523235282314698 27.857647667331044 28.112779851162212 22.50633719919205 98-99 21.198769746600902 27.804178257999308 28.218247644090376 22.778804351309407 100 20.334924896321755 28.321646947225393 28.289544451089117 23.053883705363738 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 94.0 1 62.0 2 68.5 3 132.0 4 133.0 5 140.0 6 216.0 7 424.5 8 713.0 9 891.5 10 898.5 11 868.5 12 929.0 13 1031.5 14 1175.5 15 1281.0 16 1351.0 17 1433.0 18 1559.0 19 1736.0 20 1949.5 21 2269.0 22 2703.5 23 3322.0 24 4019.0 25 4887.5 26 6204.0 27 7793.5 28 9560.0 29 12409.0 30 16480.0 31 20668.5 32 26793.0 33 35427.0 34 44722.5 35 52860.0 36 63538.5 37 73078.0 38 78901.5 39 85061.0 40 83359.0 41 79000.5 42 76907.5 43 75049.0 44 75206.5 45 77180.0 46 76567.0 47 72141.0 48 69267.5 49 66631.5 50 63333.0 51 58123.5 52 52106.5 53 47493.0 54 43176.5 55 38315.0 56 33310.0 57 28639.0 58 24244.5 59 20214.0 60 16131.5 61 12612.5 62 9876.0 63 7634.5 64 5696.5 65 3954.5 66 2829.0 67 2064.5 68 1516.5 69 1184.0 70 870.0 71 616.0 72 456.0 73 326.0 74 226.0 75 164.0 76 126.5 77 86.0 78 62.5 79 42.0 80 27.0 81 17.0 82 11.0 83 11.5 84 9.0 85 4.0 86 2.0 87 2.5 88 2.0 89 2.5 90 2.5 91 0.5 92 0.5 93 1.0 94 0.5 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003844927927104624 2 0.06893008472215102 3 8.915774903431013E-4 4 8.915774903431013E-4 5 0.002340390912150641 6 7.244067109037698E-4 7 0.0 8 0.001003024676635989 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.965449143305479E-4 18-19 0.0016438459978200932 20-21 0.0030369358264811888 22-23 0.0 24-25 1.1144718629288766E-4 26-27 1.671707794393315E-4 28-29 0.0011980572526485425 30-31 0.0019503257601255343 32-33 0.0030090740299079668 34-35 0.004151407689410066 36-37 0.001615984201246871 38-39 0.0017831549806862025 40-41 0.005739530094083714 42-43 0.007216205312464476 44-45 0.005572359314644383 46-47 0.00727192890561092 48-49 0.008358538971966575 50-51 0.007968473819941467 52-53 0.0040678222996904 54-55 0.0022568055224309754 56-57 0.002981212233334745 58-59 0.0036220335545188494 60-61 0.006603245787853595 62-63 0.012649255644242751 64-65 0.004012098706543956 66-67 0.009974523173213446 68-69 0.011451198391594208 70-71 0.005265879552338942 72-73 0.007466961481623473 74-75 0.0032041066059205203 76-77 0.002702594267602526 78-79 0.008358538971966575 80-81 0.007216205312464476 82-83 0.01615984201246871 84-85 0.01772010262056914 86-87 0.022150128275711424 88-89 0.019726151973841116 90-91 0.023348185528359964 92-93 0.033155537922134076 94-95 0.03755770178070315 96-97 0.02251233163116331 98-99 0.017135004892531478 100 0.01777582621371558 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1794572.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.85330622654043 #Duplication Level Percentage of deduplicated Percentage of total 1 79.79719021328675 52.54908803133067 2 13.42542529815307 17.68217286761634 3 3.7432254920632415 7.395113238114994 4 1.3600271578447092 3.5824913960783626 5 0.6105804343372785 2.010437015917343 6 0.30712726960265746 1.2135207681399025 7 0.1903469727360573 0.8774484239417766 8 0.11206997049987293 0.5904142468901987 9 0.06990511770488511 0.4143134810719936 >10 0.2947606713879509 3.6650189501313575 >50 0.04331180688311424 2.039964082784923 >100 0.04184531351060269 5.411840027333856 >500 0.0031382114923253646 1.3860104801479811 >1k 0.0010460704974417881 1.182166990500336 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3741 0.20846196196084638 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3242 0.1806558889807709 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2388 0.13306794043370787 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.5723593146443835E-5 0.0 3 0.0 0.0 0.0 5.5723593146443835E-5 0.0 4 0.0 0.0 0.0 5.5723593146443835E-5 0.0 5 5.5723593146443835E-5 0.0 0.0 5.5723593146443835E-5 0.0 6 5.5723593146443835E-5 0.0 0.0 5.5723593146443835E-5 0.0 7 5.5723593146443835E-5 0.0 0.0 5.5723593146443835E-5 0.0 8 5.5723593146443835E-5 0.0 0.0 5.5723593146443835E-5 0.0 9 5.5723593146443835E-5 0.0 0.0 1.1144718629288767E-4 0.0 10-11 5.5723593146443835E-5 0.0 0.0 1.1144718629288767E-4 0.0 12-13 5.5723593146443835E-5 0.0 0.0 1.1144718629288767E-4 0.0 14-15 5.5723593146443835E-5 0.0 0.0 1.1144718629288767E-4 8.358538971966575E-5 16-17 1.1144718629288767E-4 0.0 0.0 1.393089828661096E-4 1.671707794393315E-4 18-19 1.1144718629288767E-4 0.0 0.0 1.671707794393315E-4 1.671707794393315E-4 20-21 1.1144718629288767E-4 0.0 0.0 2.2289437258577534E-4 1.671707794393315E-4 22-23 1.1144718629288767E-4 0.0 0.0 2.786179657322192E-4 1.671707794393315E-4 24-25 1.1144718629288767E-4 0.0 0.0 3.9006515202510685E-4 1.671707794393315E-4 26-27 1.1144718629288767E-4 0.0 0.0 4.457887451715507E-4 1.671707794393315E-4 28-29 1.1144718629288767E-4 0.0 0.0 8.079921006234356E-4 1.671707794393315E-4 30-31 1.1144718629288767E-4 0.0 0.0 0.0034827245716527394 1.671707794393315E-4 32-33 1.1144718629288767E-4 0.0 0.0 0.009612319817761561 1.671707794393315E-4 34-35 1.1144718629288767E-4 0.0 0.0 0.018806712686924795 1.671707794393315E-4 36-37 1.1144718629288767E-4 0.0 0.0 0.035607376020577605 1.671707794393315E-4 38-39 1.1144718629288767E-4 0.0 0.0 0.06327414001778697 1.671707794393315E-4 40-41 1.393089828661096E-4 0.0 0.0 0.0977670441754357 1.671707794393315E-4 42-43 1.671707794393315E-4 0.0 0.0 0.13131264724959488 1.671707794393315E-4 44-45 1.671707794393315E-4 0.0 0.0 0.1709599837732897 1.671707794393315E-4 46-47 1.9503257601255342E-4 0.0 0.0 0.21893799747237783 1.671707794393315E-4 48-49 2.5075616915899723E-4 0.0 0.0 0.2751352411605664 1.671707794393315E-4 50-51 2.786179657322192E-4 0.0 0.0 0.3390780642961107 1.671707794393315E-4 52-53 2.786179657322192E-4 0.0 0.0 0.40146062682355454 1.671707794393315E-4 54-55 2.786179657322192E-4 0.0 0.0 0.4703071261559859 1.671707794393315E-4 56-57 2.786179657322192E-4 0.0 0.0 0.5532516945544675 1.671707794393315E-4 58-59 2.786179657322192E-4 0.0 0.0 0.6529412026934556 1.671707794393315E-4 60-61 2.786179657322192E-4 0.0 0.0 0.7627445429885231 1.671707794393315E-4 62-63 2.786179657322192E-4 0.0 0.0 0.8781759661913815 1.671707794393315E-4 64-65 2.786179657322192E-4 0.0 0.0 0.9965886016275747 1.671707794393315E-4 66-67 2.786179657322192E-4 0.0 0.0 1.130603843144772 1.671707794393315E-4 68-69 2.786179657322192E-4 0.0 0.0 1.2860448062267773 1.671707794393315E-4 70-71 2.786179657322192E-4 0.0 0.0 1.4715486478112887 1.671707794393315E-4 72-73 2.786179657322192E-4 0.0 0.0 1.6551857490253943 1.671707794393315E-4 74-75 2.786179657322192E-4 0.0 0.0 1.8490481295818726 1.671707794393315E-4 76-77 2.786179657322192E-4 0.0 0.0 2.0538880579881997 1.671707794393315E-4 78-79 2.786179657322192E-4 0.0 0.0 2.2919949715029544 1.671707794393315E-4 80-81 2.786179657322192E-4 0.0 0.0 2.5662664969697513 1.671707794393315E-4 82-83 2.786179657322192E-4 0.0 0.0 2.8632175248471503 1.671707794393315E-4 84-85 2.786179657322192E-4 0.0 0.0 3.1479093622323315 1.671707794393315E-4 86-87 2.786179657322192E-4 0.0 0.0 3.4298150199601913 1.671707794393315E-4 88 2.786179657322192E-4 0.0 0.0 3.661485858466531 1.671707794393315E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3100 0.0 42.01632 1 GTATCAA 5425 0.0 34.93053 1 GTACATG 9325 0.0 29.19642 1 ATCAACG 6400 0.0 27.82792 3 CAACGCA 6480 0.0 27.6294 5 TACATGG 9850 0.0 27.449316 2 TCAACGC 6560 0.0 27.364092 4 ACATGGG 9430 0.0 27.05887 3 AACGCAG 6720 0.0 26.991526 6 CATGGGG 3615 0.0 25.478231 4 TATCAAC 7345 0.0 24.519234 2 ACGCAGA 7650 0.0 23.525927 7 CGCAGAG 7720 0.0 23.130005 8 CATGGGA 5370 0.0 22.40202 4 ATGGGGG 1910 0.0 20.912554 5 GACATAT 655 0.0 20.818844 1 TGCGTAT 465 0.0 20.218824 94 GCAGAGT 8860 0.0 19.782652 9 TATAGGG 715 0.0 19.729458 2 AGAGTAC 8325 0.0 19.191286 10-11 >>END_MODULE