FastQCFastQC Report
Mon 6 Jun 2016
ERR1211465_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211465_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15692762
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT343800.21908189265853903No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT270970.1726719617617345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171000.10896743352126285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA207700.046.2511141
GTATCAA364700.039.678511
TCAACGC432300.032.598964
ATCAACG433350.032.5103763
CAACGCA436300.032.2441865
TATCAAC460200.031.000462
AACGCAG456000.030.937516
GTACATG562500.029.911561
TACATGG579250.028.4606672
ACATGGG586800.027.1000043
ACGCAGA522200.026.7918117
CGCAGAG539550.025.7395428
CATGGGG369200.025.2899934
GCAGAGT616400.022.0827279
ATGGGGG232150.021.9809255
GAGTACT318700.019.424112-13
AGAGTAC559300.018.88445710-11
TACCTGG124550.018.8011672
CAGAGTA602900.018.48122810-11
CATGGGA237150.017.4964564
Produced by FastQC (version 0.11.5)