FastQCFastQC Report
Mon 6 Jun 2016
ERR1211465_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211465_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15692762
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT380500.24246847049614337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306530.19533209004253044No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216200.13777052121226332No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA213750.048.4011081
GTATCAA378000.039.9970441
TCAACGC476500.031.2221494
ATCAACG479600.030.9322533
CAACGCA482200.030.7845675
TATCAAC504850.029.6855142
AACGCAG504050.029.5331486
GTACATG586050.028.1329961
TACATGG597750.027.2667792
ACATGGG608350.025.915243
ACGCAGA587700.025.1295957
CATGGGG384850.024.4448494
CGCAGAG608150.024.1841268
GCAGAGT684200.021.0700869
ATGGGGG228350.021.0516055
TACCTGG116750.019.6556682
AGAGTAC623850.019.43333810-11
GAGTACT360250.018.80992912-13
CAGAGTA668900.017.8786410-11
AGTACTT394950.017.47260712-13
Produced by FastQC (version 0.11.5)