##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211465_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15692762 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08034990908548 33.0 31.0 34.0 30.0 34.0 2 32.30665901897958 34.0 31.0 34.0 30.0 34.0 3 32.3237330050631 34.0 31.0 34.0 30.0 34.0 4 35.96681336274647 37.0 35.0 37.0 35.0 37.0 5 35.88517196654101 37.0 35.0 37.0 35.0 37.0 6 35.89785577580288 37.0 35.0 37.0 35.0 37.0 7 35.820370690640694 37.0 35.0 37.0 35.0 37.0 8 35.813582146979606 37.0 35.0 37.0 35.0 37.0 9 37.45865131963385 39.0 37.0 39.0 34.0 39.0 10-11 37.41633693928449 39.0 37.0 39.0 34.0 39.0 12-13 37.32808204827168 39.0 37.0 39.0 34.0 39.0 14-15 38.55574780271313 40.0 38.0 41.0 34.0 41.0 16-17 38.47819679543983 40.0 38.0 41.0 34.0 41.0 18-19 38.44976152062971 40.0 38.0 41.0 34.0 41.0 20-21 38.38138305417492 40.0 38.0 41.0 34.0 41.0 22-23 38.27720203110198 40.0 38.0 41.0 34.0 41.0 24-25 38.16019159023759 40.0 37.5 41.0 33.0 41.0 26-27 37.98096740395349 40.0 37.0 41.0 33.0 41.0 28-29 38.02571539031816 40.0 37.5 41.0 33.0 41.0 30-31 37.964882759325604 40.0 37.0 41.0 33.0 41.0 32-33 37.805276343323115 40.0 37.0 41.0 33.0 41.0 34-35 37.702140802237366 40.0 37.0 41.0 33.0 41.0 36-37 37.65920110175634 40.0 37.0 41.0 32.5 41.0 38-39 37.57038190600227 40.0 37.0 41.0 32.0 41.0 40-41 37.4095386777675 40.0 36.5 41.0 31.5 41.0 42-43 37.260770156330665 40.0 36.0 41.0 31.0 41.0 44-45 37.08206786670186 39.0 36.0 41.0 31.0 41.0 46-47 36.836419108376205 39.0 36.0 41.0 30.0 41.0 48-49 36.630441760347864 39.0 35.0 41.0 30.0 41.0 50-51 36.43233692704955 39.0 35.0 40.0 29.5 41.0 52-53 36.22284569790837 39.0 35.0 40.0 29.0 41.0 54-55 36.02070476822372 38.5 35.0 40.0 28.0 41.0 56-57 35.815191933708036 38.0 34.0 40.0 28.0 41.0 58-59 35.5441815468813 38.0 34.0 40.0 27.5 41.0 60-61 35.21850997294167 38.0 34.0 40.0 26.5 41.0 62-63 34.88479975672861 37.0 33.5 40.0 26.0 41.0 64-65 34.555638325490435 37.0 33.0 39.5 26.0 41.0 66-67 34.20615268363848 36.0 33.0 39.0 26.0 40.5 68-69 33.798917297031586 36.0 32.0 39.0 25.5 40.0 70-71 33.40599780969086 35.0 32.0 38.5 25.0 40.0 72-73 32.99501387964719 35.0 31.0 37.5 24.0 40.0 74-75 32.58641413793187 35.0 31.0 37.0 23.5 39.0 76-77 31.043086201141648 33.5 29.5 35.5 21.5 37.5 78-79 31.902105760604794 34.0 31.0 36.0 23.0 38.0 80-81 31.884769424273433 34.0 31.0 36.0 23.0 37.5 82-83 31.60878422804093 34.0 31.0 35.0 22.5 37.0 84-85 31.315728200045346 34.0 30.5 35.0 21.5 36.5 86-87 31.027695092807754 34.0 30.0 35.0 20.0 36.0 88-89 30.7509597736842 34.0 30.0 35.0 20.0 36.0 90-91 30.465648144029714 34.0 29.0 35.0 19.5 35.0 92-93 30.260521219910174 34.0 29.0 35.0 19.0 35.0 94-95 30.03919571328489 34.0 29.0 35.0 18.5 35.0 96-97 29.834281307522538 34.0 29.0 35.0 18.0 35.0 98-99 29.674714495765627 33.0 29.0 35.0 18.0 35.0 100 27.74230438210941 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30662267371384644 1101 2 0.251117659827905 1101 3 0.2997528636312907 1101 4 0.17732283566404305 1101 5 0.17784900441365892 1101 6 0.18565221037818702 1101 7 0.1993120365687986 1101 8 0.20429102838611612 1101 9 0.22774566458284085 1101 10-11 0.20128288392496074 1101 12-13 0.2041031016132493 1101 14-15 0.2686010373877892 1101 16-17 0.30153428391142256 1101 18-19 0.2814112203476995 1101 20-21 0.2461868328417367 1101 22-23 0.2430476764070093 1101 24-25 0.20301371940620072 1101 26-27 0.19173894352538667 1101 28-29 0.29843387050180326 1101 30-31 0.3118268914451434 1101 32-33 0.3296788772732455 1101 34-35 0.32815309813915405 1101 36-37 0.33883729414662866 1101 38-39 0.29853941354111413 1101 40-41 0.3060961700630003 1101 42-43 0.3404655718191947 1101 44-45 0.31470470011192475 1101 46-47 0.3631317330276147 1101 48-49 0.3147277746722068 1101 50-51 0.2903252663753406 1101 52-53 0.2844301360321069 1101 54-55 0.2540806861242899 1101 56-57 0.2431011103354308 1101 58-59 0.3021301012299844 1101 60-61 0.21902639337007912 1101 62-63 0.19473207190106478 1101 64-65 0.20435890816757052 1101 66-67 0.11450689224923138 1101 68-69 0.16773284237218178 1101 70-71 0.14295042321840157 1101 72-73 -0.00877631067395157 1101 74-75 0.030542591649812323 1101 76-77 0.10318981940644889 1101 78-79 0.16181342150875366 1101 80-81 0.16483439127390298 1101 82-83 0.17419270312684176 1101 84-85 0.1211085860502159 1101 86-87 0.104716908120146 1101 88-89 0.05739104017301955 1101 90-91 -0.03760581571514976 1101 92-93 -0.054050391976876 1101 94-95 -0.03140521514346517 1101 96-97 -0.13213073535003161 1101 98-99 -0.2201060989359256 1101 100 -0.08548427823712501 1102 1 0.33511445050558564 1102 2 0.2619734324359868 1102 3 0.33768010824984174 1102 4 0.22695241723808834 1102 5 0.2349189713437667 1102 6 0.17594677028790784 1102 7 0.2042243056827715 1102 8 0.2061860975799945 1102 9 0.25088558783286885 1102 10-11 0.2485393772220874 1102 12-13 0.2401807862259986 1102 14-15 0.32712471483102235 1102 16-17 0.3448191736830779 1102 18-19 0.3273830629493588 1102 20-21 0.34512341913194433 1102 22-23 0.3076544468999387 1102 24-25 0.31663359642968913 1102 26-27 0.29306465220153655 1102 28-29 0.3217791205853544 1102 30-31 0.3513653649188342 1102 32-33 0.3978275994694016 1102 34-35 0.38991634479876325 1102 36-37 0.4368655640566885 1102 38-39 0.40532710950342477 1102 40-41 0.39672184861441906 1102 42-43 0.33579302596209715 1102 44-45 0.3735011308197045 1102 46-47 0.3763868496457192 1102 48-49 0.40027822258422674 1102 50-51 0.38792349428805295 1102 52-53 0.3627906653081254 1102 54-55 0.377693149628314 1102 56-57 0.36089340581663265 1102 58-59 0.38286337677758553 1102 60-61 0.33338316182062044 1102 62-63 0.4187259208019398 1102 64-65 0.3692957584409342 1102 66-67 0.3494140862610138 1102 68-69 0.2821104217667383 1102 70-71 0.2843206431977734 1102 72-73 0.305510590101882 1102 74-75 0.20795341997399674 1102 76-77 0.3038787042036901 1102 78-79 0.3452985005195046 1102 80-81 0.3314540217709627 1102 82-83 0.22439435524958284 1102 84-85 0.20565601100762976 1102 86-87 0.2023527036323749 1102 88-89 0.16819577980555067 1102 90-91 0.1869120736619969 1102 92-93 0.1294325595359247 1102 94-95 0.12266527480752387 1102 96-97 0.13584159976499066 1102 98-99 0.12125945596229926 1102 100 0.1685395509472869 1103 1 0.26482149447895154 1103 2 0.24634934676420528 1103 3 0.28301056095062194 1103 4 0.14472739791753497 1103 5 0.14693430620978631 1103 6 0.19454899858957475 1103 7 0.18284022283120294 1103 8 0.21126843153578534 1103 9 0.23182681212796297 1103 10-11 0.23082310792815974 1103 12-13 0.26160950227659185 1103 14-15 0.3192611855401992 1103 16-17 0.3269544359820813 1103 18-19 0.33986975643916395 1103 20-21 0.31764504623944134 1103 22-23 0.31603549855255864 1103 24-25 0.3119492074474337 1103 26-27 0.3080966855477598 1103 28-29 0.3371995346481782 1103 30-31 0.41799448685367935 1103 32-33 0.4515695991997646 1103 34-35 0.4507068231235607 1103 36-37 0.40835548280928435 1103 38-39 0.4368609449255345 1103 40-41 0.478691962919342 1103 42-43 0.5106927293195938 1103 44-45 0.4340083030888593 1103 46-47 0.5023473269318615 1103 48-49 0.4763483637306436 1103 50-51 0.4823733650147233 1103 52-53 0.39486615293540694 1103 54-55 0.48991876888275954 1103 56-57 0.508202378021565 1103 58-59 0.5230476980283285 1103 60-61 0.5041434937816263 1103 62-63 0.5964698140037328 1103 64-65 0.5453033973133472 1103 66-67 0.5337228103142593 1103 68-69 0.4930363068569221 1103 70-71 0.5528968357352966 1103 72-73 0.5091078625099072 1103 74-75 0.4537877194708173 1103 76-77 0.4801579250998991 1103 78-79 0.5004606205897488 1103 80-81 0.4317583153586675 1103 82-83 0.4233655816097155 1103 84-85 0.38286846258188945 1103 86-87 0.3713862858853574 1103 88-89 0.4120744563134089 1103 90-91 0.4284853741996777 1103 92-93 0.37032162565386884 1103 94-95 0.44300808698138283 1103 96-97 0.4403875474981227 1103 98-99 0.4307459709934456 1103 100 0.5045862170167155 1104 1 0.2269803288540544 1104 2 0.19234998652043345 1104 3 0.24256576777589345 1104 4 0.05216521285540665 1104 5 0.12305209669718664 1104 6 0.1700239451464256 1104 7 0.19094032874353672 1104 8 0.16492350826869995 1104 9 0.20704161261626552 1104 10-11 0.2347748889183876 1104 12-13 0.23404108466227314 1104 14-15 0.32786367556674634 1104 16-17 0.27223273879605614 1104 18-19 0.2907695798542065 1104 20-21 0.19477953719776764 1104 22-23 0.291868135489608 1104 24-25 0.309217794466754 1104 26-27 0.3566855932308144 1104 28-29 0.37967587421223215 1104 30-31 0.3009379112495125 1104 32-33 0.3476254795077196 1104 34-35 0.410069795597785 1104 36-37 0.3062795688263904 1104 38-39 0.403526615628806 1104 40-41 0.5002806479594994 1104 42-43 0.545239003866449 1104 44-45 0.4602802757934512 1104 46-47 0.4420082078346539 1104 48-49 0.41974576552185994 1104 50-51 0.39930677708067464 1104 52-53 0.48601467474274784 1104 54-55 0.47040735171324144 1104 56-57 0.5095104106649089 1104 58-59 0.5445209661507491 1104 60-61 0.42557020007862434 1104 62-63 0.4803363226041455 1104 64-65 0.5798812137419418 1104 66-67 0.54707185368229 1104 68-69 0.5413501193613399 1104 70-71 0.41938023869752783 1104 72-73 0.5721373159272289 1104 74-75 0.5596037926136006 1104 76-77 0.5091262352725217 1104 78-79 0.5789898171547705 1104 80-81 0.6778958635919246 1104 82-83 0.48874238981855456 1104 84-85 0.541023295502324 1104 86-87 0.5790618699124437 1104 88-89 0.6075695502009388 1104 90-91 0.4388706912951541 1104 92-93 0.4701506494331902 1104 94-95 0.534931525192988 1104 96-97 0.43476069344866985 1104 98-99 0.4964759603796729 1104 100 0.6311658313309643 1105 1 0.21940021517207953 1105 2 0.13087326856437898 1105 3 0.21295377013966998 1105 4 0.11408459479066835 1105 5 0.08693999960294008 1105 6 0.11324207803717456 1105 7 0.1346448420101396 1105 8 0.15854444777790633 1105 9 0.13684682640911205 1105 10-11 0.1735716950422983 1105 12-13 0.20817765197730154 1105 14-15 0.2869142141069787 1105 16-17 0.3125224953286079 1105 18-19 0.2910226352466907 1105 20-21 0.24861538341792766 1105 22-23 0.31153886978000145 1105 24-25 0.28763618617405484 1105 26-27 0.2999294240122481 1105 28-29 0.35629907272433314 1105 30-31 0.3814746460795817 1105 32-33 0.4242389321004154 1105 34-35 0.4734028742411951 1105 36-37 0.4634005829694132 1105 38-39 0.4285779872307458 1105 40-41 0.45502983480550085 1105 42-43 0.4999649816903755 1105 44-45 0.4469040610595414 1105 46-47 0.4976009986268721 1105 48-49 0.5076835960344255 1105 50-51 0.5878635524612577 1105 52-53 0.5776922592248397 1105 54-55 0.48118122542766173 1105 56-57 0.518992335901487 1105 58-59 0.5241174889562998 1105 60-61 0.5770871525944301 1105 62-63 0.5293105573334671 1105 64-65 0.5468989527337769 1105 66-67 0.5082965865883224 1105 68-69 0.5808311064363139 1105 70-71 0.5868519737581082 1105 72-73 0.5410753202092451 1105 74-75 0.59428414870235 1105 76-77 0.5296395934365101 1105 78-79 0.6352551311922809 1105 80-81 0.5900329344837338 1105 82-83 0.623261852803239 1105 84-85 0.6168145638935947 1105 86-87 0.6469585792864194 1105 88-89 0.6262247197131998 1105 90-91 0.7221160598878917 1105 92-93 0.6409265800105217 1105 94-95 0.6983372291510968 1105 96-97 0.7180701890949273 1105 98-99 0.7168852406356301 1105 100 0.8136877902768838 1106 1 0.23252517214474722 1106 2 0.19316937941594148 1106 3 0.20847374617722636 1106 4 0.1388313622816426 1106 5 0.145509997366986 1106 6 0.11464030306904505 1106 7 0.14244563094287344 1106 8 0.16740921707720702 1106 9 0.17179263408529977 1106 10-11 0.16508554675446874 1106 12-13 0.19637150819490756 1106 14-15 0.2519238060600415 1106 16-17 0.3044003382735099 1106 18-19 0.25729258945645483 1106 20-21 0.26697737261121546 1106 22-23 0.3188370839301129 1106 24-25 0.28961674847729313 1106 26-27 0.286840729800069 1106 28-29 0.3270246516983164 1106 30-31 0.3684884674607716 1106 32-33 0.4216464807623481 1106 34-35 0.41107276035528173 1106 36-37 0.393475091205282 1106 38-39 0.36324461937815755 1106 40-41 0.3850447232839258 1106 42-43 0.3729790963684394 1106 44-45 0.4799059100999372 1106 46-47 0.5044256053458867 1106 48-49 0.5087714009274862 1106 50-51 0.47444056293473835 1106 52-53 0.5266232081107347 1106 54-55 0.44237994508240774 1106 56-57 0.511396919062463 1106 58-59 0.6043277736167241 1106 60-61 0.5952460099397427 1106 62-63 0.6374468688456005 1106 64-65 0.6577248448473583 1106 66-67 0.6895442920409636 1106 68-69 0.6414885749266617 1106 70-71 0.6799407786189491 1106 72-73 0.6657819795092053 1106 74-75 0.6446928630323256 1106 76-77 0.6255864551191017 1106 78-79 0.7347358831371906 1106 80-81 0.7158599212008703 1106 82-83 0.6558186624158964 1106 84-85 0.664392269934158 1106 86-87 0.6110300732034482 1106 88-89 0.6722725911544174 1106 90-91 0.7086724815289465 1106 92-93 0.7214035318363159 1106 94-95 0.7861776349792109 1106 96-97 0.7886704136571581 1106 98-99 0.8806785388878815 1106 100 1.028659083767895 1107 1 0.2160789337683866 1107 2 0.16251473636553726 1107 3 0.23245105266941835 1107 4 0.14492960519744713 1107 5 0.12908259510457043 1107 6 0.128273347087962 1107 7 0.16317628860918632 1107 8 0.1458972227882711 1107 9 0.16661051694308782 1107 10-11 0.18401119314673053 1107 12-13 0.19946367586812386 1107 14-15 0.2556806857761913 1107 16-17 0.20126303503132448 1107 18-19 0.2152407445045199 1107 20-21 0.2539336895956623 1107 22-23 0.2707279272474281 1107 24-25 0.3022902320659355 1107 26-27 0.3280152025875438 1107 28-29 0.36616788726958305 1107 30-31 0.25508930727493606 1107 32-33 0.24216102216660573 1107 34-35 0.276534526042866 1107 36-37 0.3742379131985558 1107 38-39 0.39946161263544866 1107 40-41 0.3811567380259504 1107 42-43 0.36011473829302787 1107 44-45 0.3592660631100202 1107 46-47 0.37150427299651057 1107 48-49 0.4196405967964054 1107 50-51 0.4106153520385689 1107 52-53 0.4265418503852061 1107 54-55 0.40706438101871356 1107 56-57 0.43439844539339845 1107 58-59 0.5190338253269999 1107 60-61 0.42971203991679374 1107 62-63 0.41988835201504315 1107 64-65 0.5131057968906632 1107 66-67 0.5109755994886882 1107 68-69 0.4838646420038799 1107 70-71 0.5444386929160032 1107 72-73 0.4588949507847744 1107 74-75 0.5305607041297122 1107 76-77 0.5417769568733455 1107 78-79 0.6247560323146075 1107 80-81 0.5302153759468986 1107 82-83 0.5270969572168056 1107 84-85 0.4438876772372744 1107 86-87 0.38025621190431025 1107 88-89 0.43156945442659733 1107 90-91 0.349625087476344 1107 92-93 0.43017710995512104 1107 94-95 0.5128917392895005 1107 96-97 0.5778599544008998 1107 98-99 0.6329462035696842 1107 100 0.7086550497714796 1108 1 0.21437711515572033 1108 2 0.180406937584209 1108 3 0.22117939482944138 1108 4 0.1216259234669792 1108 5 0.10753529280361818 1108 6 0.12264495605218428 1108 7 0.14948949064198302 1108 8 0.1257621711941468 1108 9 0.12375787386823589 1108 10-11 0.1457248164688707 1108 12-13 0.1967176364565404 1108 14-15 0.21994521102819675 1108 16-17 0.27999350814354074 1108 18-19 0.29604453355676696 1108 20-21 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84-85 -0.08416885397121021 2316 86-87 -0.0874656600671635 2316 88-89 -0.09591668529136754 2316 90-91 -0.02701651633539015 2316 92-93 0.09454797088132949 2316 94-95 0.17458975334955085 2316 96-97 0.08899215077772382 2316 98-99 -0.011112052650190662 2316 100 -0.2411868810122222 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 4.0 10 25.0 11 71.0 12 189.0 13 500.0 14 1445.0 15 3883.0 16 8495.0 17 16728.0 18 29112.0 19 45527.0 20 64885.0 21 89932.0 22 117893.0 23 139009.0 24 154670.0 25 175771.0 26 206847.0 27 249940.0 28 302434.0 29 363887.0 30 438074.0 31 531220.0 32 649581.0 33 815394.0 34 1074191.0 35 1453044.0 36 2143824.0 37 3243137.0 38 2968550.0 39 404475.0 40 25.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.698264550198466 20.145826189410908 13.771167066428935 24.384742193961692 2 14.969886603449014 21.543648499323087 39.425003712802706 24.06146118442519 3 17.32957556060237 27.00523283893349 30.489116896856476 25.17607470360766 4 12.230982441723057 17.56735125016441 38.109750191427125 32.09191611668541 5 12.854410433515874 38.54627416081753 35.20422647627004 13.39508892939655 6 30.950973408119104 37.92299030240086 17.94869915827457 13.177337131205464 7 27.05987002160614 32.768909641272835 22.751711903870078 17.419508433250947 8 26.008949178256724 35.935842173904234 20.35755802060766 17.697650627231383 9 26.849884915081862 15.099287824448982 19.932879875815342 38.11794738465382 10-11 24.718883138608742 26.34113421206541 28.88119376308645 20.0587888862394 12-13 25.36574504857717 23.926049474273555 28.844877657610557 21.863327819538714 14-15 22.709523664476656 25.270210559492334 26.110435498862465 25.909830277168545 16-17 22.491719213025256 28.275745124262308 26.687842608619793 22.544693054092647 18-19 22.471932809651626 27.570701905501892 28.319070038759005 21.638295246087473 20-21 23.252377818910418 26.773303530223252 28.138726059788983 21.835592591077344 22-23 23.018268645706495 26.583998616177272 27.959616922744924 22.438115815371308 24-25 22.77152967355647 26.725085325971975 28.036925945467843 22.46645905500371 26-27 22.70364571039966 26.907021487128063 28.027596703291664 22.361736099180614 28-29 22.668820457589657 26.928399971948636 28.07117451011511 22.3316050603466 30-31 23.02012630537888 26.77644719005501 28.02529994779361 22.1781265567725 32-33 22.831239338917708 26.86809245108946 27.958152717917816 22.342515492075012 34-35 22.947991161790565 26.961856744089417 28.02060655709237 22.069545537027647 36-37 22.97071884802421 26.93412454519826 27.925795625848494 22.169360980929042 38-39 22.887339672869725 27.004194672304173 27.857674274853792 22.250791379972313 40-41 22.913803266257577 26.87435382807646 27.89021965392476 22.321623251741205 42-43 22.77923332452613 26.906565497245744 27.796979013998357 22.51722216422977 44-45 22.78815446316358 27.014022585040575 27.746156616562693 22.451666335233153 46-47 22.740969306846647 26.961392603023203 27.62445299135059 22.67318509877956 48-49 22.78674652526269 27.035627536659884 27.646223071717873 22.53140286635955 50-51 22.859208127469195 27.135854478967964 27.423293950490002 22.581643443072842 52-53 22.856291113435905 27.169153091140274 27.356858067858457 22.61769772756536 54-55 22.706060284249606 27.111206825987328 27.491918800010744 22.690814089752323 56-57 22.78862661080955 27.155719130637596 27.268821507453918 22.786832751098938 58-59 22.790494479079946 27.107605303794625 27.455823590558232 22.6460766265672 60-61 22.927727634616858 27.18683027578976 27.406307198099984 22.479134891493405 62-63 22.77980302971061 27.174473852740427 27.457518384925077 22.588204732623886 64-65 22.696265645469015 26.99729157796793 27.47679526397986 22.829647512583193 66-67 22.693970222130325 27.090194058567736 27.36256937121478 22.85326634808716 68-69 22.600752439194444 27.132445333059373 27.412902027654866 22.853900200091314 70-71 22.624864388668193 26.954119143221885 27.406046455548033 23.014970012561893 72-73 22.57888502706078 27.21831721820155 27.182301175624428 23.02049657911324 74-75 22.58144625215201 27.203709689177728 27.16493911158534 23.04990494708492 76-77 22.46523996407546 27.087960790547566 27.295339059885816 23.151460185491153 78-79 22.424182751310056 27.076271233696886 27.302930756412785 23.19661525858027 80-81 22.47903218919813 27.25980776081433 27.064691296343145 23.196468753644396 82-83 22.512633692994307 27.204654752776808 27.114739244228538 23.16797231000035 84-85 22.44472134943189 27.198082051617394 27.083347275246638 23.273849323704077 86-87 22.306387818175672 27.18412988054891 27.183674156193693 23.325808145081727 88-89 22.25562888066385 27.045772752162005 27.232719591150428 23.465878776023715 90-91 22.393599788081403 27.060038015065675 27.18615150713782 23.360210689715107 92-93 22.380829064673954 26.989269639724245 27.113406711299877 23.516494584301924 94-95 22.19496909711717 26.96198914175828 27.128586897361274 23.714454863763272 96-97 22.114989287418023 26.907047218251794 27.221077295448225 23.756886198881954 98-99 22.003293119678155 26.838915816716337 27.16505697216865 23.99273409143686 100 21.248582768156137 27.311106872012136 27.32033572899079 24.11997463084094 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1074.0 1 866.0 2 1044.5 3 1546.5 4 1523.0 5 1620.0 6 2442.0 7 4516.0 8 7556.5 9 9714.0 10 9878.5 11 9690.5 12 10444.0 13 11398.0 14 12411.5 15 13419.0 16 14322.0 17 15209.5 18 16286.0 19 17679.5 20 19861.5 21 22763.5 22 26543.5 23 32829.5 24 41276.5 25 52146.0 26 66911.0 27 86096.0 28 108762.5 29 133965.0 30 163893.5 31 194136.0 32 225834.5 33 261583.0 34 296959.5 35 329623.0 36 372229.5 37 416981.0 38 447683.5 39 471660.0 40 489511.0 41 507340.0 42 526056.5 43 545879.0 44 565765.5 45 584554.5 46 600795.5 47 610029.0 48 620841.0 49 628172.5 50 625983.5 51 610628.0 52 585702.5 53 563019.0 54 534264.5 55 494592.5 56 449833.0 57 400977.0 58 349355.5 59 298214.5 60 249633.0 61 206826.5 62 169199.0 63 132232.5 64 98474.5 65 73842.5 66 55489.0 67 41753.5 68 32345.0 69 25185.5 70 19434.5 71 15182.0 72 11913.0 73 9408.5 74 7488.5 75 5651.5 76 4118.0 77 2774.5 78 1892.0 79 1373.5 80 951.0 81 612.5 82 431.0 83 331.0 84 250.0 85 171.0 86 114.5 87 88.0 88 62.0 89 38.0 90 30.0 91 19.5 92 12.0 93 11.0 94 7.0 95 8.5 96 8.0 97 6.0 98 8.5 99 12.0 100 11.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003307257192838329 2 0.06715834981757832 3 8.475244829431555E-4 4 6.754706405411616E-4 5 0.0016376976850856464 6 7.328219213418263E-4 7 0.0 8 9.431099509442634E-4 9 1.274472906681437E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.073324632081975E-4 18-19 9.622270445444849E-4 20-21 0.0028038403946991615 22-23 3.1861822667035925E-6 24-25 1.274472906681437E-5 26-27 1.1470256160132932E-4 28-29 7.742422908089729E-4 30-31 0.001526181305751021 32-33 0.0029599633257676376 34-35 0.0042662980551161096 36-37 0.0013381965520155089 38-39 0.0015102503944175027 40-41 0.005273131651394446 42-43 0.006780195863545244 44-45 0.005266759286861038 46-47 0.0067164722182111725 48-49 0.008666415765433772 50-51 0.00818530224316153 52-53 0.003718274705243092 54-55 0.00204871519749041 56-57 0.002179348670425257 58-59 0.002867564040033233 60-61 0.0072326337454171555 62-63 0.013518971357623342 64-65 0.0038170463555109034 66-67 0.009848489386380804 68-69 0.012273174091342239 70-71 0.005467488769663365 72-73 0.007286798843951115 74-75 0.002867564040033233 76-77 0.0026986963798979425 78-79 0.007420618499152667 80-81 0.005429254582462921 82-83 0.015781160766982893 84-85 0.016969606752463333 86-87 0.022006960916121712 88-89 0.020869493846908532 90-91 0.023698823699741318 92-93 0.033547313086122126 94-95 0.037444013998300614 96-97 0.02230646204919185 98-99 0.01769287012700505 100 0.01812937709754344 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.5692762E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.64619655950564 #Duplication Level Percentage of deduplicated Percentage of total 1 69.58553011502542 36.634134961330275 2 16.14594852268584 17.00045559129957 3 6.149201069170839 9.711961445144707 4 2.8948015236455733 6.096011600584052 5 1.5767438349891947 4.150478293041495 6 0.9679304855926928 3.057471516027039 7 0.6262999811216404 2.308061833794119 8 0.4378413686727523 1.8440546205622959 9 0.31306488950969 1.483350813810635 >10 1.2224524577463927 10.678286164626188 >50 0.050490763593975266 1.8179923819304267 >100 0.026762748090473742 2.6903066830715736 >500 0.001900367680681415 0.6775021201057911 >1k 9.699601887626593E-4 0.8685887219738437 >5k 0.0 0.0 >10k+ 6.191228612425499E-5 0.9813432526980078 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 38050 0.24246847049614337 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30653 0.19533209004253044 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21620 0.13777052121226332 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 6.372364533407185E-6 0.0 4 0.0 0.0 0.0 3.1861822667035926E-5 6.372364533407185E-6 5 0.0 0.0 0.0 3.1861822667035926E-5 6.372364533407185E-6 6 0.0 0.0 0.0 3.823418720044311E-5 5.097891626725748E-5 7 0.0 0.0 0.0 3.823418720044311E-5 5.097891626725748E-5 8 0.0 0.0 0.0 3.823418720044311E-5 5.735128080066466E-5 9 0.0 0.0 0.0 7.009600986747903E-5 6.372364533407185E-5 10-11 0.0 0.0 0.0 7.646837440088622E-5 6.372364533407185E-5 12-13 0.0 0.0 0.0 8.6026921200997E-5 6.372364533407185E-5 14-15 0.0 0.0 0.0 1.0195783253451496E-4 9.558546800110777E-5 16-17 0.0 0.0 0.0 1.0195783253451496E-4 1.338196552015509E-4 18-19 0.0 0.0 0.0 1.0514401480121854E-4 1.5293674880177243E-4 20-21 0.0 0.0 0.0 1.4019201973495805E-4 1.5930911333517962E-4 22-23 0.0 0.0 0.0 2.2940512320265865E-4 1.752400246686976E-4 24-25 0.0 3.1861822667035925E-6 0.0 3.2817677347047003E-4 1.8479857146880835E-4 26-27 0.0 6.372364533407185E-6 0.0 4.715549754721317E-4 1.9435711826891914E-4 28-29 0.0 6.372364533407185E-6 0.0 0.0011948183500138472 1.9754330053562273E-4 30-31 6.372364533407185E-6 6.372364533407185E-6 0.0 0.004502075542852176 2.0072948280232633E-4 32-33 6.372364533407185E-6 6.372364533407185E-6 0.0 0.01255037194854545 2.1347421186914068E-4 34-35 6.372364533407185E-6 6.372364533407185E-6 0.0 0.02504020643402353 2.1666039413584427E-4 36-37 6.372364533407185E-6 1.274472906681437E-5 0.0 0.04499526597038813 2.2303275866925146E-4 38-39 6.372364533407185E-6 1.9117093600221554E-5 0.0 0.07757716582969906 2.4214985226947303E-4 40-41 6.372364533407185E-6 1.9117093600221554E-5 0.0 0.1158368424882758 2.612669458696946E-4 42-43 6.372364533407185E-6 1.9117093600221554E-5 0.0 0.15365682599404745 2.612669458696946E-4 44-45 6.372364533407185E-6 2.230327586692515E-5 0.0 0.20113731413246438 2.803840394699161E-4 46-47 6.372364533407185E-6 2.548945813362874E-5 0.0 0.2583802647360611 2.995011330701377E-4 48-49 6.372364533407185E-6 2.548945813362874E-5 0.0 0.3217884780257293 3.409215025372844E-4 50-51 6.372364533407185E-6 2.548945813362874E-5 0.0 0.39356041976549444 3.4729386707069154E-4 52-53 6.372364533407185E-6 2.548945813362874E-5 0.0 0.4668904046336776 3.8871423653783827E-4 54-55 6.372364533407185E-6 2.548945813362874E-5 0.0 0.5483005477302212 4.365069705383922E-4 56-57 6.372364533407185E-6 3.1861822667035926E-5 0.0 0.6432901996474553 4.588102464053173E-4 58-59 6.372364533407185E-6 3.1861822667035926E-5 0.0 0.7540673846962058 4.8429970453894606E-4 60-61 6.372364533407185E-6 3.5048004933739514E-5 0.0 0.8752570133925437 4.906720690723532E-4 62-63 6.372364533407185E-6 4.460655173385029E-5 0.0 1.0037334409328327 5.034167981391676E-4 64-65 6.372364533407185E-6 4.460655173385029E-5 0.0 1.1344752440647479 5.034167981391676E-4 66-67 6.372364533407185E-6 4.460655173385029E-5 0.0 1.2848630470531575 5.066029804058712E-4 68-69 6.372364533407185E-6 4.460655173385029E-5 0.0 1.4591599617709106 5.16161527205982E-4 70-71 6.372364533407185E-6 4.460655173385029E-5 0.0 1.6571110936366715 5.320924385394999E-4 72-73 9.558546800110777E-6 4.460655173385029E-5 0.0 1.8595069497644836 6.40422635607422E-4 74-75 1.274472906681437E-5 4.460655173385029E-5 0.0 2.0622182379367 6.499811824075329E-4 76-77 1.274472906681437E-5 4.460655173385029E-5 0.0 2.2820775590683144 6.563535469409401E-4 78-79 1.274472906681437E-5 4.460655173385029E-5 0.0 2.537797361611678 6.627259114743472E-4 80-81 1.274472906681437E-5 4.460655173385029E-5 0.0 2.8183343378303958 7.041462809414939E-4 82-83 1.274472906681437E-5 4.460655173385029E-5 0.0 3.1144804209736945 7.58311379475455E-4 84-85 1.274472906681437E-5 4.460655173385029E-5 0.0 3.411642896260072 7.58311379475455E-4 86-87 1.274472906681437E-5 4.460655173385029E-5 0.0 3.717245568370947 7.58311379475455E-4 88 1.274472906681437E-5 4.460655173385029E-5 0.0 3.9659812593856962 7.774284730756765E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 21375 0.0 48.401108 1 GTATCAA 37800 0.0 39.997044 1 TCAACGC 47650 0.0 31.222149 4 ATCAACG 47960 0.0 30.932253 3 CAACGCA 48220 0.0 30.784567 5 TATCAAC 50485 0.0 29.685514 2 AACGCAG 50405 0.0 29.533148 6 GTACATG 58605 0.0 28.132996 1 TACATGG 59775 0.0 27.266779 2 ACATGGG 60835 0.0 25.91524 3 ACGCAGA 58770 0.0 25.129595 7 CATGGGG 38485 0.0 24.444849 4 CGCAGAG 60815 0.0 24.184126 8 GCAGAGT 68420 0.0 21.070086 9 ATGGGGG 22835 0.0 21.051605 5 TACCTGG 11675 0.0 19.655668 2 AGAGTAC 62385 0.0 19.433338 10-11 GAGTACT 36025 0.0 18.809929 12-13 CAGAGTA 66890 0.0 17.87864 10-11 AGTACTT 39495 0.0 17.472607 12-13 >>END_MODULE