FastQCFastQC Report
Mon 6 Jun 2016
ERR1211464_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211464_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13920457
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241110.17320552047967966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198980.14294070948963816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA183200.041.868711
GTATCAA306400.035.914011
TCAACGC347550.030.5273024
ATCAACG350350.030.3242153
CAACGCA351250.030.191275
AACGCAG369750.028.7526976
GTACATG433850.028.1746831
TATCAAC384050.027.7187232
TACATGG438850.027.0431862
ACATGGG437350.025.9250323
ACGCAGA420550.024.9675647
CGCAGAG430850.024.2078178
CATGGGG228100.022.5976434
GCAGAGT500900.020.3553229
TACCTGG111750.018.639782
GTACCTG118950.018.5653171
AGAGTAC441700.018.51480110-11
CATGGGA214050.018.3954564
GAGTACT269500.018.28149612-13
ATGGGGG135900.017.8400655
Produced by FastQC (version 0.11.5)