FastQCFastQC Report
Mon 6 Jun 2016
ERR1211464_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211464_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13920457
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT281680.2023496786060975No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237330.1704900923870531No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT176650.1268995694609739No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA199500.041.7414781
GTATCAA335200.035.0693861
TCAACGC399600.028.4116744
ATCAACG402750.028.1196523
CAACGCA405950.027.9670455
GTACATG460750.026.8910311
AACGCAG424850.026.8439856
TATCAAC434000.026.3805752
TACATGG469700.025.62682
ACATGGG461050.025.0021843
ACGCAGA479500.023.5787017
CGCAGAG486250.023.1257558
CATGGGG244700.022.9103554
GCAGAGT568300.019.4891649
AGAGTAC501600.019.21448710-11
TACCTGG103200.018.7256582
ATGGGGG139600.018.4803055
GAGTACT303700.018.33595512-13
GTACCTG117250.017.4850061
CAGAGTA547150.017.17687810-11
Produced by FastQC (version 0.11.5)