##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211464_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 13920457 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02243884665568 33.0 31.0 34.0 30.0 34.0 2 32.25603239893633 34.0 31.0 34.0 30.0 34.0 3 32.27591745012394 34.0 31.0 34.0 30.0 34.0 4 35.93494444902204 37.0 35.0 37.0 35.0 37.0 5 35.854030223289364 37.0 35.0 37.0 35.0 37.0 6 35.866362146012875 37.0 35.0 37.0 35.0 37.0 7 35.78726323424583 37.0 35.0 37.0 33.0 37.0 8 35.778139467691325 37.0 35.0 37.0 33.0 37.0 9 37.420154525099285 39.0 37.0 39.0 34.0 39.0 10-11 37.372203944166486 39.0 37.0 39.0 34.0 39.0 12-13 37.290111416600766 39.0 37.0 39.0 34.0 39.0 14-15 38.507219123625035 40.0 38.0 41.0 34.0 41.0 16-17 38.431409040665834 40.0 38.0 41.0 34.0 41.0 18-19 38.406090080232275 40.0 38.0 41.0 33.5 41.0 20-21 38.33971952213925 40.0 38.0 41.0 34.0 41.0 22-23 38.235355168296564 40.0 38.0 41.0 33.5 41.0 24-25 38.12384097734722 40.0 37.5 41.0 33.0 41.0 26-27 37.94511803743225 40.0 37.0 41.0 33.0 41.0 28-29 37.9868352023213 40.0 37.0 41.0 33.0 41.0 30-31 37.92743496136657 40.0 37.0 41.0 33.0 41.0 32-33 37.769609000624044 40.0 37.0 41.0 33.0 41.0 34-35 37.66555645407331 40.0 37.0 41.0 32.5 41.0 36-37 37.619121161036595 40.0 37.0 41.0 32.0 41.0 38-39 37.52935234094686 40.0 37.0 41.0 32.0 41.0 40-41 37.37538559258507 40.0 36.5 41.0 31.0 41.0 42-43 37.229629278693935 39.5 36.0 41.0 31.0 41.0 44-45 37.05472266463666 39.0 36.0 41.0 31.0 41.0 46-47 36.8187928744006 39.0 36.0 41.0 30.0 41.0 48-49 36.62325076683905 39.0 35.0 40.0 30.0 41.0 50-51 36.43458364908566 39.0 35.0 40.0 29.5 41.0 52-53 36.237657930339495 39.0 35.0 40.0 29.0 41.0 54-55 36.042880776112455 38.5 35.0 40.0 28.0 41.0 56-57 35.850306207619475 38.0 34.5 40.0 28.0 41.0 58-59 35.59213070375492 38.0 34.0 40.0 27.5 41.0 60-61 35.27900179570254 38.0 34.0 40.0 26.5 41.0 62-63 34.95989298339846 37.5 33.5 40.0 26.0 41.0 64-65 34.64825317875699 37.0 33.0 40.0 26.0 41.0 66-67 34.30924918628749 36.5 33.0 39.0 26.0 40.5 68-69 33.90622100265817 36.0 32.0 39.0 25.5 40.0 70-71 33.51992064628338 35.5 32.0 38.5 25.0 40.0 72-73 33.103872667398775 35.0 31.0 38.0 24.0 40.0 74-75 32.68660906750404 35.0 31.0 37.0 23.0 39.0 76-77 31.140787870685568 33.5 29.5 35.5 21.5 38.5 78-79 31.998212019907108 34.0 31.0 36.0 23.0 39.0 80-81 31.964936100876574 34.0 31.0 36.0 23.0 37.5 82-83 31.669015571830727 34.0 31.0 35.5 22.5 37.0 84-85 31.35083359691424 34.0 30.0 35.0 21.5 37.0 86-87 31.04578969641586 34.0 30.0 35.0 20.0 36.0 88-89 30.745584106901088 34.0 30.0 35.0 20.0 36.0 90-91 30.442385260771253 34.0 29.0 35.0 19.5 35.5 92-93 30.22229654529302 34.0 29.0 35.0 19.0 35.0 94-95 29.98476379044165 34.0 29.0 35.0 18.5 35.0 96-97 29.76557457847828 33.5 29.0 35.0 18.0 35.0 98-99 29.589338446288078 33.0 29.0 35.0 18.0 35.0 100 27.64830493711521 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22194519150172454 1101 2 0.16848217601146587 1101 3 0.2368360158861904 1101 4 0.13165961755689182 1101 5 0.11733063552325262 1101 6 0.13603396007558644 1101 7 0.16022071136492855 1101 8 0.16837636754172536 1101 9 0.18075297924970357 1101 10-11 0.16682498274181512 1101 12-13 0.1743420333078589 1101 14-15 0.21650796627501023 1101 16-17 0.22275068652685093 1101 18-19 0.2632296939757097 1101 20-21 0.2497254140418761 1101 22-23 0.2159580771046663 1101 24-25 0.20717255199530626 1101 26-27 0.1578339152368642 1101 28-29 0.26133761852637605 1101 30-31 0.24151938293653785 1101 32-33 0.3183333039729206 1101 34-35 0.2595531653560954 1101 36-37 0.25922931859678755 1101 38-39 0.2510663216554647 1101 40-41 0.2585044199196531 1101 42-43 0.21758921089939065 1101 44-45 0.2241451224825468 1101 46-47 0.2634926061416323 1101 48-49 0.23194170944041304 1101 50-51 0.20089105987747047 1101 52-53 0.17180808865229835 1101 54-55 0.15996400048970827 1101 56-57 0.16759411283415204 1101 58-59 0.2310085603866483 1101 60-61 0.1825386141294203 1101 62-63 0.1579443930352724 1101 64-65 0.18823164397561953 1101 66-67 0.12873384906406926 1101 68-69 0.1240327280194009 1101 70-71 0.06135813247770017 1101 72-73 -0.049721348337527616 1101 74-75 -0.03704535840997636 1101 76-77 0.11751837509886798 1101 78-79 0.23079125915790044 1101 80-81 0.1977669551626775 1101 82-83 0.13855334758804716 1101 84-85 0.19190195328095427 1101 86-87 0.1252706768796621 1101 88-89 0.017558453441886 1101 90-91 -0.021553711733986347 1101 92-93 -0.13159504352455542 1101 94-95 -0.10000675149575144 1101 96-97 -0.16658983202886546 1101 98-99 -0.18126766699534969 1101 100 -0.06269269235962938 1102 1 0.22327651282810024 1102 2 0.18291939327132667 1102 3 0.2644411111288605 1102 4 0.17088434525395968 1102 5 0.14199950412066897 1102 6 0.13754533026229154 1102 7 0.1458063569033925 1102 8 0.1302077688273684 1102 9 0.17070508203309487 1102 10-11 0.15877193385535548 1102 12-13 0.20061609906206712 1102 14-15 0.25281340689638654 1102 16-17 0.2524947937054378 1102 18-19 0.20332357421161618 1102 20-21 0.23873074058965926 1102 22-23 0.2026482340283806 1102 24-25 0.22365431650111844 1102 26-27 0.20807873438754854 1102 28-29 0.2591731885890667 1102 30-31 0.2579780663060305 1102 32-33 0.3167219847052394 1102 34-35 0.25004834174664836 1102 36-37 0.25173921423675694 1102 38-39 0.29159162609516187 1102 40-41 0.27633891584587644 1102 42-43 0.23942223860853318 1102 44-45 0.23297459857184322 1102 46-47 0.2627680828521548 1102 48-49 0.24685109234149394 1102 50-51 0.25087016817403907 1102 52-53 0.22023775164237236 1102 54-55 0.25037480459386074 1102 56-57 0.2770316269472133 1102 58-59 0.2504334583683274 1102 60-61 0.2834813664606557 1102 62-63 0.3263366850347751 1102 64-65 0.24868860672065551 1102 66-67 0.20657653452420988 1102 68-69 0.30864855985353046 1102 70-71 0.302876812533988 1102 72-73 0.24412954208285953 1102 74-75 0.18303097410939984 1102 76-77 0.2164059162106149 1102 78-79 0.2800727840470607 1102 80-81 0.25184639479168425 1102 82-83 0.17680197690535593 1102 84-85 0.11035306467150008 1102 86-87 0.06245392357446278 1102 88-89 0.04270269357404288 1102 90-91 0.07217407021330757 1102 92-93 0.004762142336833364 1102 94-95 0.057793305049031574 1102 96-97 0.1140671657609289 1102 98-99 0.016949631814142663 1102 100 0.10721704005518262 1103 1 0.22965216242879904 1103 2 0.21129643916711416 1103 3 0.26930787988509053 1103 4 0.09369309352469202 1103 5 0.11625233933528278 1103 6 0.1285546269207316 1103 7 0.16698256747631035 1103 8 0.17584879827902 1103 9 0.21567361358290782 1103 10-11 0.17439565243829236 1103 12-13 0.21046667905762462 1103 14-15 0.24214002719212857 1103 16-17 0.23619379667221096 1103 18-19 0.25335890766280045 1103 20-21 0.2370252863196214 1103 22-23 0.24144788108926463 1103 24-25 0.2547434587149482 1103 26-27 0.23379771864933474 1103 28-29 0.3111041330638713 1103 30-31 0.3822565528699471 1103 32-33 0.3899894372520265 1103 34-35 0.3397536718076424 1103 36-37 0.3844942182353748 1103 38-39 0.381505527820984 1103 40-41 0.40817384747874996 1103 42-43 0.4499311248385638 1103 44-45 0.3825116284445258 1103 46-47 0.450498950960899 1103 48-49 0.393330241412734 1103 50-51 0.4145862025050846 1103 52-53 0.3910689208046563 1103 54-55 0.42591437938853716 1103 56-57 0.46018306197412784 1103 58-59 0.4803215546765571 1103 60-61 0.47094667126808787 1103 62-63 0.47911934088457997 1103 64-65 0.4196848028016049 1103 66-67 0.43621196052544775 1103 68-69 0.46310809746183423 1103 70-71 0.4684686028478495 1103 72-73 0.40697348848372883 1103 74-75 0.3978637939839942 1103 76-77 0.4168129411387973 1103 78-79 0.4450470058981857 1103 80-81 0.41609250758372696 1103 82-83 0.39730675987390995 1103 84-85 0.3214627235336067 1103 86-87 0.337023940083629 1103 88-89 0.3721334575639723 1103 90-91 0.4270650297377223 1103 92-93 0.3471379753878807 1103 94-95 0.39512942597328404 1103 96-97 0.3252489930095983 1103 98-99 0.4051150151350491 1103 100 0.4271073461527628 1104 1 0.22125684635661003 1104 2 0.17258293234100108 1104 3 0.23221236670958945 1104 4 0.025648037002234503 1104 5 0.08110410360232123 1104 6 0.11751596312708301 1104 7 0.15470448209936905 1104 8 0.12153568962526151 1104 9 0.17844673796874844 1104 10-11 0.1866971973862448 1104 12-13 0.21619112340013658 1104 14-15 0.290832738091396 1104 16-17 0.2277311512878839 1104 18-19 0.2614403071208855 1104 20-21 0.1532169753584256 1104 22-23 0.22848944025577822 1104 24-25 0.24641959004095781 1104 26-27 0.2858338840888024 1104 28-29 0.3407841406526515 1104 30-31 0.26711695262824975 1104 32-33 0.23230252511578442 1104 34-35 0.35401301366547955 1104 36-37 0.24353061966112932 1104 38-39 0.32921448311264356 1104 40-41 0.4567088801033492 1104 42-43 0.4829160134413115 1104 44-45 0.4124813613060212 1104 46-47 0.35993376422950263 1104 48-49 0.3612872314095128 1104 50-51 0.35350212774601886 1104 52-53 0.37260959898831914 1104 54-55 0.44950958542649033 1104 56-57 0.43340446811763655 1104 58-59 0.4425195118414891 1104 60-61 0.3706316851104319 1104 62-63 0.3579983891048073 1104 64-65 0.48011600319326675 1104 66-67 0.4722529024640423 1104 68-69 0.46094599498503896 1104 70-71 0.44307481038596563 1104 72-73 0.49140026772909806 1104 74-75 0.5306633009020985 1104 76-77 0.5032389964422315 1104 78-79 0.542565551963758 1104 80-81 0.6368970264035454 1104 82-83 0.4196603339957754 1104 84-85 0.48374083380361554 1104 86-87 0.5583745956563213 1104 88-89 0.5326674993757798 1104 90-91 0.4112272506025185 1104 92-93 0.3699259700343944 1104 94-95 0.41869221951943203 1104 96-97 0.42836009522024554 1104 98-99 0.4776893718891486 1104 100 0.6747308392487206 1105 1 0.23048618891387918 1105 2 0.15911906418437383 1105 3 0.21338804654075716 1105 4 0.1249525938977385 1105 5 0.12054475665186004 1105 6 0.15353549865285743 1105 7 0.17978748906911335 1105 8 0.1852620654415844 1105 9 0.1712774309390639 1105 10-11 0.18912173224176598 1105 12-13 0.20005361558986579 1105 14-15 0.3100784062848092 1105 16-17 0.316404124304583 1105 18-19 0.2753974182219494 1105 20-21 0.2617010749313451 1105 22-23 0.3167085271180312 1105 24-25 0.3123751020048857 1105 26-27 0.313543509920315 1105 28-29 0.37757891911632413 1105 30-31 0.4146660702118865 1105 32-33 0.40912064662784076 1105 34-35 0.42823094776547066 1105 36-37 0.4636545484861969 1105 38-39 0.44758764028907905 1105 40-41 0.4392781198326432 1105 42-43 0.445119838378389 1105 44-45 0.43867925362160776 1105 46-47 0.4851014774565314 1105 48-49 0.5440715170522665 1105 50-51 0.5973624469986945 1105 52-53 0.5907430856389766 1105 54-55 0.5617842598989213 1105 56-57 0.5769818043712789 1105 58-59 0.6760751557217617 1105 60-61 0.6266673070815045 1105 62-63 0.6165335017198146 1105 64-65 0.6408171180463427 1105 66-67 0.5473820698269165 1105 68-69 0.6143235161039584 1105 70-71 0.665371183684762 1105 72-73 0.638389713131545 1105 74-75 0.6476188586392411 1105 76-77 0.6484539016804938 1105 78-79 0.7185957654682618 1105 80-81 0.6785488526257097 1105 82-83 0.6738669719205355 1105 84-85 0.660134393701739 1105 86-87 0.6989681916395689 1105 88-89 0.6573808532290464 1105 90-91 0.7453694218600724 1105 92-93 0.6948085944364379 1105 94-95 0.7670013165670753 1105 96-97 0.7332221311405185 1105 98-99 0.7452024916795423 1105 100 0.8958017509048695 1106 1 0.25961053686762625 1106 2 0.19823015505108543 1106 3 0.23145328209486848 1106 4 0.14873419503967966 1106 5 0.15341266630984052 1106 6 0.13985789455932007 1106 7 0.1570320965250076 1106 8 0.17854118750572212 1106 9 0.1679885508296195 1106 10-11 0.17923631574540622 1106 12-13 0.2292585902462534 1106 14-15 0.28638766266596605 1106 16-17 0.289267625186838 1106 18-19 0.332623485054647 1106 20-21 0.3261416083295785 1106 22-23 0.3263536899878048 1106 24-25 0.3370613912559648 1106 26-27 0.3696978730281515 1106 28-29 0.38363163556770274 1106 30-31 0.37787970364816914 1106 32-33 0.42536720636698533 1106 34-35 0.44511197638598077 1106 36-37 0.4397191247675778 1106 38-39 0.4162084287768977 1106 40-41 0.44841879959734143 1106 42-43 0.45363704486550915 1106 44-45 0.5183918126531921 1106 46-47 0.5571389259674433 1106 48-49 0.5199304424655367 1106 50-51 0.5595739214284734 1106 52-53 0.6056512092475117 1106 54-55 0.549665290541995 1106 56-57 0.5534170203553614 1106 58-59 0.6093855673260364 1106 60-61 0.6193941228540965 1106 62-63 0.6594938460821567 1106 64-65 0.7169266522102831 1106 66-67 0.7372675554276711 1106 68-69 0.612052594202531 1106 70-71 0.6516521759496214 1106 72-73 0.6437811764304584 1106 74-75 0.6649670497813034 1106 76-77 0.6638903936518297 1106 78-79 0.7170677768425087 1106 80-81 0.7147825842306759 1106 82-83 0.7318149141519648 1106 84-85 0.6745830868956091 1106 86-87 0.5731241110287932 1106 88-89 0.6786364024378635 1106 90-91 0.7499323509288018 1106 92-93 0.8132358990635851 1106 94-95 0.8507865743424574 1106 96-97 0.8136418155761689 1106 98-99 0.8388732923617539 1106 100 0.975490694354388 1107 1 0.20462745877437527 1107 2 0.16488972645753108 1107 3 0.22209291560780997 1107 4 0.13375443390643227 1107 5 0.10274949719190118 1107 6 0.11944775641100591 1107 7 0.15904574579938213 1107 8 0.1747751271800908 1107 9 0.19836793936871544 1107 10-11 0.162158283890264 1107 12-13 0.19684295845780042 1107 14-15 0.22342306838841353 1107 16-17 0.20714987476840463 1107 18-19 0.21113397990015415 1107 20-21 0.270633949376915 1107 22-23 0.3287040053041679 1107 24-25 0.35399620071550686 1107 26-27 0.3996333758854931 1107 28-29 0.3622014748643778 1107 30-31 0.27309022460736543 1107 32-33 0.2457488143239459 1107 34-35 0.28595225472907515 1107 36-37 0.37107820071376807 1107 38-39 0.3786682641644674 1107 40-41 0.33485019593700116 1107 42-43 0.32616832836939835 1107 44-45 0.375624642947578 1107 46-47 0.3212170040479734 1107 48-49 0.4304038039750324 1107 50-51 0.38734461952646626 1107 52-53 0.4228279962781656 1107 54-55 0.3972016660720783 1107 56-57 0.5031553766426029 1107 58-59 0.5848703327095066 1107 60-61 0.5871850061120156 1107 62-63 0.5158247012785111 1107 64-65 0.5767409543225526 1107 66-67 0.6240176079968833 1107 68-69 0.5160811208176455 1107 70-71 0.6385139513497151 1107 72-73 0.5700298452678894 1107 74-75 0.5783851094594823 1107 76-77 0.48572701142516195 1107 78-79 0.6145093848280645 1107 80-81 0.6295990897141195 1107 82-83 0.6393913906876314 1107 84-85 0.481820637780924 1107 86-87 0.4275613317599358 1107 88-89 0.45036112932246297 1107 90-91 0.3270839772751728 1107 92-93 0.4727765363719101 1107 94-95 0.6340711063064397 1107 96-97 0.679354005480505 1107 98-99 0.6708764439882628 1107 100 0.8870449124066049 1108 1 0.2294762224883229 1108 2 0.16673137116975312 1108 3 0.20094543099675377 1108 4 0.14667092899477296 1108 5 0.09993268710467618 1108 6 0.11320768874690401 1108 7 0.1219314449431863 1108 8 0.11987707392700742 1108 9 0.13959027882231823 1108 10-11 0.15180242655204523 1108 12-13 0.20840220518136476 1108 14-15 0.1918545870487307 1108 16-17 0.2479689167468706 1108 18-19 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-0.2807041817702718 2316 80-81 -0.2122088643584661 2316 82-83 -0.11977870216209041 2316 84-85 -0.1545631487266803 2316 86-87 -0.16675002068581435 2316 88-89 -0.06644918611526762 2316 90-91 0.0058944214570075815 2316 92-93 0.075799571836356 2316 94-95 0.08475114200954792 2316 96-97 0.1046570338941244 2316 98-99 -0.0039022598463844815 2316 100 -0.09048530458909809 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 8.0 10 13.0 11 57.0 12 158.0 13 418.0 14 1300.0 15 3312.0 16 7263.0 17 15053.0 18 26241.0 19 40641.0 20 57407.0 21 80156.0 22 103235.0 23 118475.0 24 131785.0 25 152342.0 26 181675.0 27 221748.0 28 268799.0 29 326235.0 30 394034.0 31 478102.0 32 585111.0 33 733599.0 34 955590.0 35 1291267.0 36 1891162.0 37 2851068.0 38 2657315.0 39 346850.0 40 36.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.90616249114585 20.789776164114706 13.711021966922463 24.593039377816986 2 14.619439171397076 21.900365857796217 39.973393075154966 23.506801895651744 3 16.994404885276655 27.26448558291389 30.933335191523092 24.80777434028636 4 11.611252778033798 17.98995673030747 39.28120573257875 31.117584759079985 5 12.166613686201194 38.930031662562925 36.19598821139745 12.707366439838436 6 30.25984771684459 39.18978815526928 18.04410711658529 12.506257011300836 7 26.356720903631253 32.955419495207664 23.41130036176255 17.276559239398534 8 25.06165981254325 36.98298553389284 20.70976616863493 17.245588484928987 9 26.214371487362627 15.015561276314878 20.432246021896418 38.337821214426086 10-11 24.07322906137349 26.77038907558854 29.73070136993347 19.4256804931045 12-13 24.8266094999611 24.410028348925614 29.354399068938612 21.408963082174672 14-15 22.04194876648087 25.837463525802352 26.68770500853528 25.432882699181498 16-17 21.830822359361058 28.70895886184569 27.330255491918958 22.129963286874293 18-19 21.707943784496102 28.103258519818162 28.87138003653435 21.31741765915138 20-21 22.579283826607625 27.36114610731017 28.696320894242433 21.36324917183977 22-23 22.219611755562337 27.385031252925103 28.41747221373551 21.977884777777053 24-25 21.994352124468914 27.45576105609475 28.68327117600426 21.86661564343207 26-27 22.07494756720487 27.614923912059236 28.56299749465507 21.747131026080822 28-29 22.01989447676085 27.52103288237232 28.700913756023017 21.758158884843812 30-31 22.262385484416356 27.466818703980316 28.620478328468845 21.650317483134483 32-33 22.048718033635055 27.569383496236117 28.565244793108306 21.816653677020525 34-35 22.290373570474596 27.592280183854545 28.61450251463425 21.502843731036613 36-37 22.257861609918862 27.515961576701198 28.499169271015923 21.727007542364017 38-39 22.160890322898585 27.70244908623428 28.541334701316135 21.595325889551003 40-41 22.279203654608754 27.508816796062053 28.5847134059051 21.627266143424098 42-43 22.12570443235161 27.48792506879358 28.4695450805454 21.91682541830941 44-45 22.189342119687932 27.682027844433595 28.325160063753945 21.80346997212453 46-47 22.170740990642788 27.620789296878456 28.185363038171985 22.02310667430677 48-49 22.138138362511633 27.67228679880209 28.248709256676246 21.940865582010023 50-51 22.226529167701646 27.779916882058842 28.0096433910209 21.983910559218614 52-53 22.223903662273415 27.797545773613425 27.951073662531677 22.027476901581483 54-55 22.150805428844865 27.75728112934318 28.086296696834417 22.005616744977537 56-57 22.23368881816165 27.801210246100904 27.888669829852336 22.07643110588511 58-59 22.183914949113305 27.723653583541374 28.07548439325942 22.01694707408591 60-61 22.328178287425676 27.846928564465063 27.938763753097184 21.886129395012077 62-63 22.100077974757102 27.808770662179054 28.066185071537692 22.02496629152616 64-65 22.06347872189104 27.69267683625073 28.042822023658147 22.20102241820008 66-67 22.071948966771387 27.786507995351418 27.877965136796316 22.263577901080875 68-69 22.085654442753764 27.806286758258302 27.91723373497923 22.190825064008703 70-71 22.063644559338254 27.588348983919424 27.97365146824507 22.37435498849725 72-73 22.0379361167923 27.730745966470945 27.809136110297807 22.422181806438953 74-75 22.04767727632394 27.79151354093455 27.717185607442552 22.44362357529896 76-77 21.920597284545824 27.703942916073203 27.88605412812174 22.489405671259235 78-79 21.920497370172956 27.625000166134424 27.863364443634865 22.59113802005776 80-81 21.868440620675088 27.87033455319791 27.68375385860105 22.57747096752595 82-83 22.003437913685733 27.828470739115517 27.69888258824248 22.46920875895627 84-85 21.942129521524052 27.737885422990843 27.667930005065326 22.652055050419776 86-87 21.76948702579809 27.726383006424555 27.868803988520384 22.63532597925697 88-89 21.836095996233855 27.611113957213103 27.833205713800822 22.719584332752227 90-91 21.913858521838335 27.718383734102716 27.694244737166958 22.67351300689199 92-93 21.864071796927725 27.62404694490787 27.709405699774535 22.80247555838987 94-95 21.686465553795433 27.543600747273533 27.78739562857273 22.982538070358306 96-97 21.724197766824265 27.525468119045225 27.793470763217265 22.956863350913245 98-99 21.619272064812613 27.46097732422907 27.728786992270173 23.190963618688148 100 20.892593725416315 27.878564362194258 27.831804847570663 23.39703706481876 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 402.0 1 351.0 2 472.0 3 738.5 4 783.5 5 892.0 6 1358.0 7 2558.5 8 4323.0 9 5701.0 10 6179.0 11 6453.0 12 7218.0 13 8125.0 14 9104.5 15 10036.5 16 10931.0 17 12189.5 18 13628.0 19 15165.0 20 17428.0 21 20938.0 22 25728.5 23 32519.5 24 41750.0 25 53411.5 26 69001.5 27 87211.0 28 107819.5 29 133120.0 30 163796.5 31 196194.0 32 232379.5 33 274262.0 34 316558.0 35 355948.0 36 400527.5 37 440285.0 38 466244.5 39 490326.0 40 501786.0 41 505886.0 42 514202.0 43 524143.5 44 535231.5 45 548815.5 46 554978.5 47 552239.5 48 551891.0 49 544365.0 50 527019.5 51 500619.0 52 467752.0 53 436455.0 54 403762.5 55 365285.0 56 325469.0 57 286227.5 58 247372.0 59 209323.0 60 172151.5 61 139774.0 62 111741.0 63 85979.0 64 64316.0 65 48291.5 66 35958.5 67 27089.0 68 20906.0 69 16252.0 70 12754.0 71 9835.0 72 7682.0 73 6193.0 74 4934.5 75 3694.5 76 2570.5 77 1711.5 78 1186.5 79 822.0 80 561.0 81 399.5 82 275.5 83 183.0 84 133.5 85 104.0 86 71.5 87 44.0 88 31.5 89 24.5 90 18.0 91 9.5 92 8.0 93 10.5 94 12.0 95 8.5 96 4.5 97 6.5 98 8.5 99 9.5 100 8.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0031392647525867864 2 0.06494758038475318 3 7.1118354806886E-4 4 6.465304982444182E-4 5 0.001666611951030056 6 5.244080707982504E-4 7 0.0 8 9.051426975421855E-4 9 3.59183610135788E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.824488592579971E-4 18-19 9.590202390625538E-4 20-21 0.002115591463699791 22-23 0.0 24-25 1.0775508304073638E-5 26-27 1.3289793575024155E-4 28-29 7.973876145014492E-4 30-31 0.0013648977185159942 32-33 0.00284473419227544 34-35 0.0038791829894665097 36-37 0.001099101847015511 38-39 0.0014654691293540148 40-41 0.005157876641549914 42-43 0.006580243737687635 44-45 0.0049639174920765895 46-47 0.0066736314763229405 48-49 0.008275590377528555 50-51 0.007754774142831662 52-53 0.0036672646594863947 54-55 0.002119183299801149 56-57 0.0022484893994500323 58-59 0.002974040291924324 60-61 0.006867590625796265 62-63 0.013289793575024154 64-65 0.0037139585288040473 66-67 0.009834447245517873 68-69 0.011824324445670139 70-71 0.005344652118820524 72-73 0.007022039578154653 74-75 0.0027836729785523564 76-77 0.0028124076673632196 78-79 0.007223182399830696 80-81 0.00533387661051645 82-83 0.015315589136189996 84-85 0.016572731771665256 86-87 0.020940404470916434 88-89 0.0200137107567661 90-91 0.02257828173313563 92-93 0.032222361665281536 94-95 0.03642480990387025 96-97 0.021960485923702075 98-99 0.016978609251118694 100 0.017485058141410156 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.3920457E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.96021701423357 #Duplication Level Percentage of deduplicated Percentage of total 1 70.62401294181605 39.52135090640066 2 16.60497844303229 18.58436394377514 3 5.9619808170818684 10.009012210757968 4 2.6576540111640856 5.948915808539622 5 1.3919962572078772 3.894820631817685 6 0.8014683718609842 2.691020641163108 7 0.4972446383065956 1.9478142508160818 8 0.30987364263234485 1.3872477030957662 9 0.23136236947378003 1.1652379564212016 >10 0.8553602388528116 7.789736316274593 >50 0.03600937287533208 1.3875446760929988 >100 0.02424669646263615 2.711551616244124 >500 0.0024987110627590274 0.9547855954620644 >1k 0.0012488904062644814 1.1683494926935196 >5k 0.0 0.0 >10k+ 6.459776436259925E-5 0.8382482504453661 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28168 0.2023496786060975 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 23733 0.1704900923870531 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17665 0.1268995694609739 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 7.183672202715758E-6 0.0 3 0.0 0.0 0.0 7.183672202715758E-6 0.0 4 0.0 0.0 0.0 7.183672202715758E-6 0.0 5 0.0 7.183672202715758E-6 0.0 7.183672202715758E-6 0.0 6 0.0 7.183672202715758E-6 0.0 7.183672202715758E-6 7.183672202715758E-6 7 0.0 7.183672202715758E-6 0.0 7.183672202715758E-6 7.183672202715758E-6 8 0.0 7.183672202715758E-6 0.0 7.183672202715758E-6 7.183672202715758E-6 9 0.0 7.183672202715758E-6 0.0 7.183672202715758E-6 7.183672202715758E-6 10-11 0.0 7.183672202715758E-6 0.0 7.183672202715758E-6 7.183672202715758E-6 12-13 0.0 1.4367344405431516E-5 0.0 7.183672202715758E-6 7.183672202715758E-6 14-15 0.0 1.7959180506789395E-5 0.0 1.0775508304073638E-5 2.8734688810863033E-5 16-17 0.0 2.1551016608147275E-5 0.0 1.4367344405431516E-5 4.310203321629455E-5 18-19 0.0 2.1551016608147275E-5 0.0 1.4367344405431516E-5 6.465304982444183E-5 20-21 7.183672202715758E-6 4.310203321629455E-5 0.0 1.7959180506789395E-5 7.183672202715758E-5 22-23 7.183672202715758E-6 4.310203321629455E-5 0.0 4.310203321629455E-5 8.261223033123122E-5 24-25 7.183672202715758E-6 4.310203321629455E-5 0.0 1.0775508304073638E-4 8.62040664325891E-5 26-27 7.183672202715758E-6 4.310203321629455E-5 0.0 2.3706118268962002E-4 9.338773863530486E-5 28-29 7.183672202715758E-6 4.310203321629455E-5 0.0 9.159182058462592E-4 1.0775508304073638E-4 30-31 7.183672202715758E-6 6.465304982444183E-5 0.0 0.004055182958433046 1.0775508304073638E-4 32-33 7.183672202715758E-6 6.465304982444183E-5 0.0 0.01151901837705472 1.1493875524345213E-4 34-35 7.183672202715758E-6 7.183672202715758E-5 0.0 0.022675261307872292 1.221224274461679E-4 36-37 7.183672202715758E-6 8.62040664325891E-5 0.0 0.0423728904877189 1.221224274461679E-4 38-39 7.183672202715758E-6 1.0057141083802062E-4 0.0 0.07452341543097328 1.3289793575024155E-4 40-41 7.183672202715758E-6 1.0775508304073638E-4 0.0 0.11182104150747349 1.364897718515994E-4 42-43 7.183672202715758E-6 1.0775508304073638E-4 0.0 0.14901091250093298 1.4008160795295728E-4 44-45 1.0775508304073638E-5 1.0775508304073638E-4 0.0 0.19537792473336185 1.5085711625703092E-4 46-47 2.1551016608147275E-5 1.0775508304073638E-4 0.0 0.2523624044814046 1.6522446066246244E-4 48-49 2.8734688810863033E-5 1.0775508304073638E-4 0.0 0.31605643406678385 1.7959180506789396E-4 50-51 2.8734688810863033E-5 1.0775508304073638E-4 0.0 0.3866970746721893 1.7959180506789396E-4 52-53 2.8734688810863033E-5 1.1134691914209426E-4 0.0 0.4564397562522552 1.9395914947332549E-4 54-55 2.8734688810863033E-5 1.4367344405431516E-4 0.0 0.5344185180127348 2.1551016608147277E-4 56-57 2.8734688810863033E-5 1.4367344405431516E-4 0.0 0.6280325423224252 2.226938382841885E-4 58-59 2.8734688810863033E-5 1.6522446066246244E-4 0.0 0.7356439519191073 2.226938382841885E-4 60-61 2.8734688810863033E-5 1.6522446066246244E-4 0.0 0.8549719308784187 2.2987751048690426E-4 62-63 2.8734688810863033E-5 1.724081328651782E-4 0.0 0.9778055418726554 2.3706118268962002E-4 64-65 2.8734688810863033E-5 1.8318364116925184E-4 0.0 1.1072840496544043 2.442448548923358E-4 66-67 2.8734688810863033E-5 2.0114282167604125E-4 0.0 1.253953085017252 2.4783669099369366E-4 68-69 2.8734688810863033E-5 2.0114282167604125E-4 0.0 1.426400728079545 2.5142852709505157E-4 70-71 2.8734688810863033E-5 2.0473465777739913E-4 0.0 1.619109918589598 2.5861219929776733E-4 72-73 2.8734688810863033E-5 2.119183299801149E-4 0.0 1.817770781519601 2.5861219929776733E-4 74-75 2.8734688810863033E-5 2.1551016608147277E-4 0.0 2.0181629094504583 2.5861219929776733E-4 76-77 2.8734688810863033E-5 2.1551016608147277E-4 0.0 2.2379581360008514 2.622040353991252E-4 78-79 2.8734688810863033E-5 2.2269383828418853E-4 0.0 2.4882121326907587 2.657958715004831E-4 80-81 2.8734688810863033E-5 2.4783669099369366E-4 0.0 2.7673193487828742 2.6938770760184095E-4 82-83 2.8734688810863033E-5 2.5142852709505157E-4 0.0 3.0620259090631867 2.837550520072724E-4 84-85 2.8734688810863033E-5 2.622040353991252E-4 0.0 3.356340959208451 2.873468881086303E-4 86-87 2.8734688810863033E-5 2.729795437031988E-4 0.0 3.6624264562578657 2.945305603113461E-4 88 2.8734688810863033E-5 2.729795437031988E-4 0.0 3.908011066016008 3.0171423251406184E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 19950 0.0 41.741478 1 GTATCAA 33520 0.0 35.069386 1 TCAACGC 39960 0.0 28.411674 4 ATCAACG 40275 0.0 28.119652 3 CAACGCA 40595 0.0 27.967045 5 GTACATG 46075 0.0 26.891031 1 AACGCAG 42485 0.0 26.843985 6 TATCAAC 43400 0.0 26.380575 2 TACATGG 46970 0.0 25.6268 2 ACATGGG 46105 0.0 25.002184 3 ACGCAGA 47950 0.0 23.578701 7 CGCAGAG 48625 0.0 23.125755 8 CATGGGG 24470 0.0 22.910355 4 GCAGAGT 56830 0.0 19.489164 9 AGAGTAC 50160 0.0 19.214487 10-11 TACCTGG 10320 0.0 18.725658 2 ATGGGGG 13960 0.0 18.480305 5 GAGTACT 30370 0.0 18.335955 12-13 GTACCTG 11725 0.0 17.485006 1 CAGAGTA 54715 0.0 17.176878 10-11 >>END_MODULE