FastQCFastQC Report
Mon 6 Jun 2016
ERR1211463_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211463_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2298390
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100580.43761067529879616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86960.3783518027836877No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47110.20496956565247848No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30940.13461597030965156No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27400.11921388450175992No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24770.10777109193826984No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41400.058.02371
TACCTGG19850.041.9568982
GTATCAA74400.040.897351
TATCAAC91350.033.171722
ATCAACG90600.033.101533
ACCTGGG24800.032.980193
CAACGCA91350.032.77835
TCAACGC95600.031.4686164
AACGCAG97400.031.1766286
GTACATG76000.029.3765221
TAACGCA2450.028.7793354
TACATGG76650.028.6678662
GTGGTAT16300.027.7408161
ACGCAGA108900.027.5390137
GTACTAG6600.027.1190991
CGCAGAG111650.026.8612968
ACATGGG78750.026.6219523
ATAACGC2900.025.934483
CCTGGGG24650.025.7437884
GCAGAGT116050.024.9106799
Produced by FastQC (version 0.11.5)