FastQCFastQC Report
Mon 6 Jun 2016
ERR1211463_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211463_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2298390
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91810.3994535305148386No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83020.36120936829693834No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47010.20453447848276401No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29590.1287422935185064No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24810.10794512680615562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44500.059.0670971
TACCTGG21300.044.1312032
GTATCAA77050.038.1418081
ACCTGGG24450.037.0999873
ATCAACG91000.031.556963
CAACGCA92850.030.9720785
TATCAAC96050.030.3871212
TCAACGC96050.030.0445944
GTGGTAT16350.029.9095171
TATCACG1602.1354936E-929.3748322
AACGCAG100050.028.9761436
CCTGGGG24200.027.9667824
GTACATG72550.026.832231
GTACCTG36150.026.6648671
ACGCAGA109650.026.267847
CGCAGAG111350.025.8246068
TACATGG74100.025.751542
TGGTATC18800.025.749852
ACATGGG72750.025.0019173
TGGGGGG39950.023.875466
Produced by FastQC (version 0.11.5)