##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211463_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2298390 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06103968430075 33.0 31.0 34.0 30.0 34.0 2 32.19019139484596 33.0 31.0 34.0 30.0 34.0 3 32.264090080447616 34.0 31.0 34.0 30.0 34.0 4 35.91248743685797 37.0 35.0 37.0 35.0 37.0 5 35.76914405301102 37.0 35.0 37.0 35.0 37.0 6 35.81258707181984 37.0 35.0 37.0 35.0 37.0 7 35.750511444968 37.0 35.0 37.0 33.0 37.0 8 35.735729793464124 37.0 35.0 37.0 33.0 37.0 9 37.32457067773528 39.0 37.0 39.0 34.0 39.0 10-11 37.30011508055639 39.0 37.0 39.0 34.0 39.0 12-13 37.21839396273043 39.0 37.0 39.0 33.0 39.0 14-15 38.42126988892224 40.0 38.0 41.0 33.5 41.0 16-17 38.403664956774094 40.0 38.0 41.0 33.5 41.0 18-19 38.41643367748729 40.0 38.0 41.0 33.5 41.0 20-21 38.38057662102602 40.0 38.0 41.0 34.0 41.0 22-23 38.26824538046198 40.0 38.0 41.0 34.0 41.0 24-25 38.14833383368358 40.0 37.5 41.0 33.0 41.0 26-27 38.01676804197721 40.0 37.0 41.0 33.0 41.0 28-29 37.93449066520478 40.0 37.0 41.0 33.0 41.0 30-31 37.96339002519154 40.0 37.0 41.0 33.0 41.0 32-33 37.78273943934667 40.0 37.0 41.0 33.0 41.0 34-35 37.658909497517826 40.0 37.0 41.0 33.0 41.0 36-37 37.49984315107532 40.0 36.0 41.0 32.0 41.0 38-39 37.379437127728536 40.0 36.0 41.0 32.0 41.0 40-41 37.30191394845957 40.0 36.0 41.0 32.0 41.0 42-43 37.15995696987892 39.5 36.0 41.0 31.0 41.0 44-45 36.99342779075788 39.0 36.0 41.0 31.0 41.0 46-47 36.757768481415255 39.0 35.0 41.0 30.5 41.0 48-49 36.52394654519033 39.0 35.0 41.0 30.0 41.0 50-51 36.33194061930308 39.0 35.0 41.0 29.5 41.0 52-53 36.119095758335185 39.0 35.0 40.0 29.0 41.0 54-55 35.912041037421844 38.0 35.0 40.0 28.0 41.0 56-57 35.677950869956796 38.0 34.5 40.0 28.0 41.0 58-59 35.408449392835855 38.0 34.0 40.0 27.5 41.0 60-61 35.14835537049848 37.5 34.0 40.0 27.0 41.0 62-63 34.85048207658404 37.0 34.0 40.0 26.0 41.0 64-65 34.50354008675638 36.5 33.5 39.5 26.0 41.0 66-67 34.1435739800469 36.0 33.0 39.0 26.0 40.5 68-69 33.76730319919596 35.5 33.0 39.0 25.5 40.0 70-71 33.39359878001558 35.0 32.5 38.0 25.0 40.0 72-73 33.03076958218579 35.0 32.0 37.5 24.5 39.5 74-75 32.659793594646686 35.0 32.0 37.0 24.0 39.0 76-77 31.63031143539608 34.0 30.5 36.0 22.5 38.0 78-79 32.10492279378174 34.5 31.0 36.0 24.0 38.0 80-81 31.991061351641804 35.0 31.0 35.5 24.0 37.0 82-83 31.720223286735497 34.0 31.0 35.0 23.0 37.0 84-85 31.451892629188258 34.0 31.0 35.0 23.0 36.5 86-87 31.19918442910037 34.0 31.0 35.0 21.5 36.0 88-89 30.961288771705412 34.0 31.0 35.0 20.0 36.0 90-91 30.736155091172517 34.0 30.0 35.0 20.0 35.0 92-93 30.556908749167896 34.0 30.0 35.0 19.5 35.0 94-95 30.3823152293562 34.0 30.0 35.0 19.0 35.0 96-97 30.21459847980543 34.0 29.5 35.0 18.5 35.0 98-99 30.012971036247112 34.0 29.0 35.0 18.0 35.0 100 28.183112091507535 32.0 25.0 34.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.31770185545482477 1101 2 -0.25835793490327674 1101 3 -0.2640853749888947 1101 4 -0.21621770236468763 1101 5 -0.1866266810638919 1101 6 -0.3110067087614752 1101 7 -0.2610568481454365 1101 8 -0.25287975282375896 1101 9 -0.3818551342381866 1101 10-11 -0.3581512013145485 1101 12-13 -0.39872541296772823 1101 14-15 -0.7012351957847542 1101 16-17 -0.6631392120357944 1101 18-19 -0.5809741204684968 1101 20-21 -0.5807085492015673 1101 22-23 -0.6748616232955342 1101 24-25 -0.6661790090423025 1101 26-27 -0.6070376424701536 1101 28-29 -0.6497662586545161 1101 30-31 -0.7147584061345924 1101 32-33 -0.6421650881254806 1101 34-35 -0.7067170818560342 1101 36-37 -0.6569589637454314 1101 38-39 -0.7123395636231677 1101 40-41 -0.64031118938059 1101 42-43 -0.6698391823810468 1101 44-45 -0.7984953246133628 1101 46-47 -0.7098745141776774 1101 48-49 -0.6615392409311625 1101 50-51 -0.8142935331856336 1101 52-53 -0.6799297692301778 1101 54-55 -0.6214712590259879 1101 56-57 -0.7345235642249932 1101 58-59 -0.6464525879779401 1101 60-61 -0.6903601253455705 1101 62-63 -0.7137633683591744 1101 64-65 -0.8885511043195677 1101 66-67 -0.8400384015556241 1101 68-69 -0.8828950420707713 1101 70-71 -0.722709770163739 1101 72-73 -0.6701946370707432 1101 74-75 -0.7105442645047901 1101 76-77 -0.7058329332352784 1101 78-79 -0.5646156765426724 1101 80-81 -0.52019217244084 1101 82-83 -0.7871571553896466 1101 84-85 -0.7858901198309844 1101 86-87 -0.8664690093112917 1101 88-89 -0.9073054498175033 1101 90-91 -1.0991900396677963 1101 92-93 -0.9541130372728901 1101 94-95 -1.2914966503040795 1101 96-97 -1.192412730492336 1101 98-99 -1.476926137112958 1101 100 -1.3528403839046028 1102 1 -0.20148400467815009 1102 2 -0.22032635863640593 1102 3 -0.311665164480452 1102 4 -0.1518407394583079 1102 5 -0.1810679424169237 1102 6 -0.24670720107961586 1102 7 -0.2300312560904274 1102 8 -0.3468849306524717 1102 9 -0.44346068554961704 1102 10-11 -0.3526152748227389 1102 12-13 -0.44064407437168285 1102 14-15 -0.47121386907225116 1102 16-17 -0.4948035444354204 1102 18-19 -0.5912167781376212 1102 20-21 -0.6184791119721211 1102 22-23 -0.7240425077705339 1102 24-25 -0.6736428279031728 1102 26-27 -0.6671842768814997 1102 28-29 -0.7063976991094805 1102 30-31 -0.44775698435375944 1102 32-33 -0.4977673290218405 1102 34-35 -0.6231938240680677 1102 36-37 -0.4421109033091355 1102 38-39 -0.5404059416895493 1102 40-41 -0.5188417682788113 1102 42-43 -0.49131390863518476 1102 44-45 -0.436681280814021 1102 46-47 -0.4479511842396491 1102 48-49 -0.49943871890417313 1102 50-51 -0.563805459168151 1102 52-53 -0.46648916571603394 1102 54-55 -0.7078821112456595 1102 56-57 -0.6312354307897934 1102 58-59 -0.6837950249527296 1102 60-61 -0.6602114749763288 1102 62-63 -0.7361967356454784 1102 64-65 -0.6244314413028462 1102 66-67 -0.6023182377325256 1102 68-69 -0.5876229938575506 1102 70-71 -0.5574945931838542 1102 72-73 -0.6245597164358259 1102 74-75 -0.6483869773474993 1102 76-77 -0.4507479446944096 1102 78-79 -0.4462481780281138 1102 80-81 -0.47801196702533844 1102 82-83 -0.512373605606097 1102 84-85 -0.537874777330348 1102 86-87 -0.5915920888461379 1102 88-89 -0.5675221122400487 1102 90-91 -0.5258754865738311 1102 92-93 -0.5573062084807674 1102 94-95 -0.7184993453214794 1102 96-97 -0.928563630246181 1102 98-99 -0.8719445990431858 1102 100 -1.1951086548493457 1103 1 -0.2949035166448013 1103 2 -0.23206117199409348 1103 3 -0.3368076246306373 1103 4 -0.14543042300162767 1103 5 -0.07841310970026427 1103 6 -0.3124941275311457 1103 7 -0.2936910378336748 1103 8 -0.25449191237073165 1103 9 -0.4311123177916656 1103 10-11 -0.43678970849485665 1103 12-13 -0.4283456652417428 1103 14-15 -0.6943521295752788 1103 16-17 -0.7969330309543992 1103 18-19 -0.6776475464076483 1103 20-21 -0.6115836504012364 1103 22-23 -0.7647263945518006 1103 24-25 -0.625866767453239 1103 26-27 -0.4885341746224299 1103 28-29 -0.702254255398806 1103 30-31 -0.5703874622392604 1103 32-33 -0.5060233826024643 1103 34-35 -0.49752679832376145 1103 36-37 -0.4109804332296747 1103 38-39 -0.5572556598916591 1103 40-41 -0.6865173650502356 1103 42-43 -0.6599910197775216 1103 44-45 -0.6288561203999947 1103 46-47 -0.7357733783601432 1103 48-49 -0.6976167901888886 1103 50-51 -0.6557937345026374 1103 52-53 -0.7454198949558872 1103 54-55 -0.9237026036503906 1103 56-57 -0.7505869834102796 1103 58-59 -0.8829707075905446 1103 60-61 -0.67957356574491 1103 62-63 -0.7196608200755605 1103 64-65 -0.7431573401678477 1103 66-67 -0.7921223842986436 1103 68-69 -0.8084174921636418 1103 70-71 -0.901431645312563 1103 72-73 -1.0123323422300885 1103 74-75 -0.8969908678756582 1103 76-77 -0.7553765335192857 1103 78-79 -0.5958590471521212 1103 80-81 -0.5287632979544128 1103 82-83 -0.7366125171324889 1103 84-85 -0.7214387790164523 1103 86-87 -0.7633752215643561 1103 88-89 -0.7892841686620713 1103 90-91 -0.9763930267358809 1103 92-93 -0.8932818848737547 1103 94-95 -0.7620097976031879 1103 96-97 -0.9310303375042324 1103 98-99 -1.1988075094455048 1103 100 -1.2792522546807348 1104 1 -0.30523083256290917 1104 2 -0.3225132522026115 1104 3 -0.385618988434274 1104 4 -0.22990214165764655 1104 5 -0.2132095555930178 1104 6 -0.38761131612165656 1104 7 -0.3416747849753392 1104 8 -0.40210461518249474 1104 9 -0.4666059311086528 1104 10-11 -0.5264484340007201 1104 12-13 -0.47598019419056214 1104 14-15 -0.6396606521742072 1104 16-17 -0.6335282297118354 1104 18-19 -0.5810411300309397 1104 20-21 -0.7676543620094449 1104 22-23 -0.6893719101689015 1104 24-25 -0.7553614923804659 1104 26-27 -0.7859097085379574 1104 28-29 -0.815739889486153 1104 30-31 -0.791096786314256 1104 32-33 -0.7975620745062315 1104 34-35 -0.839790828596108 1104 36-37 -0.7359433161070612 1104 38-39 -0.8434971990911535 1104 40-41 -1.0311707228491471 1104 42-43 -0.9463333145166573 1104 44-45 -0.9547996566910157 1104 46-47 -1.0045554037748943 1104 48-49 -1.1328392264426128 1104 50-51 -1.1082735389810594 1104 52-53 -0.8719302763295644 1104 54-55 -1.0194410503908031 1104 56-57 -0.8671698986208156 1104 58-59 -1.0276373894847524 1104 60-61 -0.9451793806338955 1104 62-63 -1.0118037914249527 1104 64-65 -1.0481986702424848 1104 66-67 -1.0956617715932921 1104 68-69 -0.9308956216129332 1104 70-71 -0.9181603641944562 1104 72-73 -1.0444557208145895 1104 74-75 -1.0011846585934592 1104 76-77 -0.990571052620961 1104 78-79 -0.8278038828596834 1104 80-81 -1.0139041399864759 1104 82-83 -1.0189305544044238 1104 84-85 -0.8975515947657868 1104 86-87 -0.8911430168453442 1104 88-89 -1.0998764437323132 1104 90-91 -0.8544693065469602 1104 92-93 -1.1327080097446363 1104 94-95 -1.179820686815237 1104 96-97 -1.175028276710826 1104 98-99 -1.284050883563264 1104 100 -1.433480053565571 1105 1 -0.4669191984152299 1105 2 -0.3614993366018382 1105 3 -0.4410893042819488 1105 4 -0.21011286565496334 1105 5 -0.3467237750538885 1105 6 -0.25846410245915763 1105 7 -0.3849065694563123 1105 8 -0.37530629963987394 1105 9 -0.544241351424624 1105 10-11 -0.4690755964717468 1105 12-13 -0.5955421113451891 1105 14-15 -0.7671005295815618 1105 16-17 -0.7517137202135231 1105 18-19 -0.7586611786008888 1105 20-21 -0.86998216866386 1105 22-23 -0.7621298397257874 1105 24-25 -0.7771100106156368 1105 26-27 -0.7565756507068357 1105 28-29 -0.7074855001972864 1105 30-31 -0.8416296735094733 1105 32-33 -0.785466893608195 1105 34-35 -0.8023152267743754 1105 36-37 -0.8240580965770832 1105 38-39 -0.8058239094660067 1105 40-41 -0.7025419779224151 1105 42-43 -0.7212024163538757 1105 44-45 -0.728842735711325 1105 46-47 -0.7942827876467149 1105 48-49 -0.8092245305767776 1105 50-51 -0.9957794076515398 1105 52-53 -0.9341036267267242 1105 54-55 -1.030764784128337 1105 56-57 -1.0114713897993397 1105 58-59 -0.902324294425398 1105 60-61 -0.9130724504788148 1105 62-63 -1.0381315408821763 1105 64-65 -0.9240495380907561 1105 66-67 -0.9348184942893809 1105 68-69 -1.1087684102859896 1105 70-71 -0.9593637931662684 1105 72-73 -1.0977898406093445 1105 74-75 -1.0922050565429373 1105 76-77 -0.9828926867448082 1105 78-79 -0.7931669301355448 1105 80-81 -0.6693802947144185 1105 82-83 -0.7735591182067019 1105 84-85 -0.7450448085569796 1105 86-87 -0.8732339794502941 1105 88-89 -0.9357717347349563 1105 90-91 -0.9847091057848054 1105 92-93 -1.041667235294625 1105 94-95 -1.1009131055654287 1105 96-97 -1.2315352917084041 1105 98-99 -1.3144370122903162 1105 100 -1.0997508679821237 1106 1 -0.2827999733536686 1106 2 -0.3270168591471325 1106 3 -0.3930293683165651 1106 4 -0.13347449272127676 1106 5 -0.18622342987578122 1106 6 -0.3381071556818185 1106 7 -0.35584841645815146 1106 8 -0.34517116377016066 1106 9 -0.5089441721005201 1106 10-11 -0.41112168726173337 1106 12-13 -0.42916694351252715 1106 14-15 -0.5660427761202627 1106 16-17 -0.7053684632868595 1106 18-19 -0.7517746035830868 1106 20-21 -0.6238800312775368 1106 22-23 -0.6906297282903395 1106 24-25 -0.6953812798382586 1106 26-27 -0.7044218208606097 1106 28-29 -0.6631719018106494 1106 30-31 -0.6223342173579596 1106 32-33 -0.6465969170368169 1106 34-35 -0.6736052416143181 1106 36-37 -0.768748061406967 1106 38-39 -0.785807995939912 1106 40-41 -0.8054366496770697 1106 42-43 -0.6076017874230644 1106 44-45 -0.6836169294350682 1106 46-47 -0.7034982277080317 1106 48-49 -0.7439824710321972 1106 50-51 -0.8315035070628269 1106 52-53 -0.7367990056888019 1106 54-55 -0.9128772877296072 1106 56-57 -0.9174501056280704 1106 58-59 -0.9722313545407459 1106 60-61 -0.9901144371826618 1106 62-63 -0.9252814025391416 1106 64-65 -0.8295869287616995 1106 66-67 -0.7920938023676385 1106 68-69 -0.9578250140595657 1106 70-71 -0.9976503643789485 1106 72-73 -0.9601558462735298 1106 74-75 -0.8252780793790535 1106 76-77 -0.780272119021955 1106 78-79 -0.7413891949387938 1106 80-81 -0.6046349383112322 1106 82-83 -0.6870033298144698 1106 84-85 -0.5745363367447176 1106 86-87 -0.6567960952018304 1106 88-89 -0.7860330644588664 1106 90-91 -0.8810636037001487 1106 92-93 -0.8531792648875332 1106 94-95 -0.886188881128998 1106 96-97 -0.8769945688456566 1106 98-99 -0.8381133086107688 1106 100 -0.9861709633273819 1107 1 -0.4186804047692867 1107 2 -0.30089995171000083 1107 3 -0.4745287628703636 1107 4 -0.3172238943151484 1107 5 -0.2701903696972394 1107 6 -0.34260224171149645 1107 7 -0.2831156716748495 1107 8 -0.39181164136865476 1107 9 -0.36748096351726645 1107 10-11 -0.48953665023095283 1107 12-13 -0.5161927946572433 1107 14-15 -0.6538280750417229 1107 16-17 -0.7345539621463644 1107 18-19 -0.6485741868108192 1107 20-21 -0.7635093010812639 1107 22-23 -0.722835418076599 1107 24-25 -0.7363360192147823 1107 26-27 -0.754842817171614 1107 28-29 -0.741705665996399 1107 30-31 -0.7191950492128782 1107 32-33 -0.7563982814027952 1107 34-35 -0.795284087605701 1107 36-37 -0.7611993441080998 1107 38-39 -0.7612117440742949 1107 40-41 -0.8377901809772297 1107 42-43 -0.8249420129869733 1107 44-45 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0.6117162379233747 2311 30-31 0.6870225125879017 2311 32-33 0.9190393425145658 2311 34-35 0.8372336796026758 2311 36-37 0.8488409822373413 2311 38-39 0.9592533613481322 2311 40-41 0.9413988777726487 2311 42-43 0.9948909079750337 2311 44-45 0.8848346454316314 2311 46-47 1.0023397086188126 2311 48-49 1.0178023550936572 2311 50-51 1.0111119029113667 2311 52-53 0.9597310348284864 2311 54-55 0.9248410295855862 2311 56-57 0.9739303615381587 2311 58-59 1.0259200955731984 2311 60-61 1.0924436371382384 2311 62-63 0.9812422635502713 2311 64-65 1.1211230812787036 2311 66-67 1.081206749170839 2311 68-69 1.0530101454242384 2311 70-71 1.1121924156923413 2311 72-73 1.205770613894508 2311 74-75 1.0554997718095223 2311 76-77 0.9447713460951555 2311 78-79 0.8849422876218114 2311 80-81 0.8802442621768343 2311 82-83 0.9220936011584122 2311 84-85 0.8976028993635445 2311 86-87 0.904266238768944 2311 88-89 0.9856341767270536 2311 90-91 1.0875616606471006 2311 92-93 1.1669321576494873 2311 94-95 1.1620514142833294 2311 96-97 1.2527657335156164 2311 98-99 1.183516144255396 2311 100 1.349408466694804 2312 1 0.09904013203784956 2312 2 0.11760087885478754 2312 3 0.33595493183150893 2312 4 0.2554942382255021 2312 5 0.3528343068196307 2312 6 0.4298967747249449 2312 7 0.4832879828102037 2312 8 0.2374313425446175 2312 9 0.33313594940457847 2312 10-11 0.3048166020858858 2312 12-13 0.3792298098190585 2312 14-15 0.8062856904767202 2312 16-17 0.8150599891563104 2312 18-19 0.8267110466215968 2312 20-21 0.9055871318615587 2312 22-23 0.4502116618324692 2312 24-25 0.5327000338539349 2312 26-27 0.8552739536799265 2312 28-29 0.9401087902022383 2312 30-31 0.8770220560502793 2312 32-33 0.7295340275673112 2312 34-35 0.8383166293028452 2312 36-37 0.8842669743042109 2312 38-39 0.892155868270514 2312 40-41 0.8954427924447046 2312 42-43 0.8017368753676379 2312 44-45 0.8330178911380557 2312 46-47 0.9994552933267187 2312 48-49 1.0032942796863864 2312 50-51 0.9871785818231587 2312 52-53 0.9982340108167307 2312 54-55 0.988987750159076 2312 56-57 0.9799850589917369 2312 58-59 1.119184626210064 2312 60-61 1.179575566954604 2312 62-63 1.160603020638284 2312 64-65 1.2149213893290494 2312 66-67 1.1391043456595398 2312 68-69 1.2423324471807433 2312 70-71 1.203319580530028 2312 72-73 1.1100171569491835 2312 74-75 1.1464428617410576 2312 76-77 1.109258001394391 2312 78-79 1.0449303220893427 2312 80-81 0.9072876130780152 2312 82-83 0.9871628196934132 2312 84-85 0.8859833533955133 2312 86-87 1.0548960483396677 2312 88-89 0.8289429012671157 2312 90-91 1.1074160039124266 2312 92-93 1.2739533346247036 2312 94-95 1.3966507016599614 2312 96-97 1.3784600953821986 2312 98-99 1.454846935488174 2312 100 1.4949922084346596 2313 1 -0.362620534086016 2313 2 0.019740613049819444 2313 3 0.2828920045843759 2313 4 0.11230177982643852 2313 5 0.24517147583562604 2313 6 0.3503829565138261 2313 7 0.16631514003714898 2313 8 0.1339529890257154 2313 9 0.26528873425012733 2313 10-11 0.42697217516952435 2313 12-13 0.5886919555113934 2313 14-15 0.552261033122555 2313 16-17 0.71834987156263 2313 18-19 0.5258532658470827 2313 20-21 0.6791122281246871 2313 22-23 0.8044663223247284 2313 24-25 0.6839915022694427 2313 26-27 0.8097696165384818 2313 28-29 0.7480074022321688 2313 30-31 0.7199628839131549 2313 32-33 0.8607118832319927 2313 34-35 0.8490582370242592 2313 36-37 0.7680597308221095 2313 38-39 0.5814631872364728 2313 40-41 0.7046872859395208 2313 42-43 0.7034394233719752 2313 44-45 0.9840219218913688 2313 46-47 0.9859087498938166 2313 48-49 0.8789645700023954 2313 50-51 0.7713375463796694 2313 52-53 0.7544544056651645 2313 54-55 0.8494040987757785 2313 56-57 0.868680025744915 2313 58-59 0.727510591385709 2313 60-61 0.6923745604324623 2313 62-63 0.8341495057620278 2313 64-65 0.9646817916834749 2313 66-67 0.8969131350765309 2313 68-69 0.9402420902438564 2313 70-71 0.8282788966377765 2313 72-73 0.8750148033793934 2313 74-75 0.5296610079761948 2313 76-77 0.8237613716639238 2313 78-79 0.6657630723310604 2313 80-81 0.7876031557560914 2313 82-83 0.7637702177477941 2313 84-85 0.8046074851190888 2313 86-87 0.9345395779310444 2313 88-89 0.9267913727189523 2313 90-91 1.0086712941436282 2313 92-93 0.7426352189223664 2313 94-95 0.6638089675667018 2313 96-97 0.9506828830265945 2313 98-99 1.0884293814620882 2313 100 1.06621022320914 2314 1 -0.2917693336282916 2314 2 -0.16545637928798484 2314 3 0.14255506282739816 2314 4 -0.25039653384164495 2314 5 0.13490050421911803 2314 6 0.18008225116185628 2314 7 0.06149738216977596 2314 8 0.20340728215713 2314 9 0.30116779677010186 2314 10-11 0.3904955974605926 2314 12-13 0.4744034804533399 2314 14-15 0.47521530444314664 2314 16-17 0.7321745115064786 2314 18-19 0.7522344237280407 2314 20-21 0.4106733303931165 2314 22-23 0.49473813594056537 2314 24-25 0.7799423984915066 2314 26-27 0.7652822521079798 2314 28-29 0.7060339891219272 2314 30-31 0.6770698655078604 2314 32-33 0.821171011265605 2314 34-35 0.7588579172673988 2314 36-37 0.7627576711700144 2314 38-39 0.7839846962615908 2314 40-41 0.7701784758874695 2314 42-43 0.6365741513808274 2314 44-45 0.7118573039206666 2314 46-47 0.8418382258557457 2314 48-49 0.6204047084149167 2314 50-51 0.8587599023749561 2314 52-53 1.0212874179228564 2314 54-55 1.0624424838007727 2314 56-57 0.8034413087895871 2314 58-59 0.8168656148247351 2314 60-61 0.9450567848120954 2314 62-63 0.7538160486649375 2314 64-65 0.8931364490049631 2314 66-67 0.8607646074087327 2314 68-69 0.8899380828342629 2314 70-71 1.0463248951627548 2314 72-73 1.0950547171911253 2314 74-75 1.0977211835381269 2314 76-77 0.898553484057647 2314 78-79 0.9414845034458779 2314 80-81 0.9488270068581421 2314 82-83 0.888458779440473 2314 84-85 0.6349905238033813 2314 86-87 0.9287195111594819 2314 88-89 1.0252828497582378 2314 90-91 1.1158301652152716 2314 92-93 1.1104070857972026 2314 94-95 1.204475886833169 2314 96-97 1.2323430723851416 2314 98-99 1.2604067306655864 2314 100 1.325496562835152 2315 1 -0.12746660293797518 2315 2 0.08426624348094691 2315 3 0.020250884267284164 2315 4 -0.08657921330569707 2315 5 0.21068694735777171 2315 6 0.34856875508743457 2315 7 0.4256007356801774 2315 8 0.37519870628265295 2315 9 0.552613207553982 2315 10-11 0.5778277694816936 2315 12-13 0.6334294044047724 2315 14-15 0.6939697921510088 2315 16-17 0.6583985614894559 2315 18-19 0.7684491884590301 2315 20-21 0.8105573631039533 2315 22-23 0.7890080830028268 2315 24-25 0.7029994791846761 2315 26-27 0.8189493364798466 2315 28-29 0.8061056940869804 2315 30-31 0.8328875086669711 2315 32-33 0.7902336139296011 2315 34-35 0.7990498288686538 2315 36-37 0.8479395303056592 2315 38-39 0.8481271973386342 2315 40-41 0.8402066365616676 2315 42-43 1.0224371093237536 2315 44-45 0.84836329507214 2315 46-47 0.7792286766035872 2315 48-49 0.8255827118103269 2315 50-51 0.7255288314037642 2315 52-53 0.6916823657228193 2315 54-55 0.6638504191502292 2315 56-57 0.8234107481138082 2315 58-59 0.8654270401109159 2315 60-61 0.8443843417481958 2315 62-63 0.9596235606698613 2315 64-65 0.9106756408339436 2315 66-67 0.9703529579584753 2315 68-69 0.9395111481280765 2315 70-71 0.6613280033615112 2315 72-73 0.8121250234667343 2315 74-75 1.007807927857307 2315 76-77 0.8951920064435619 2315 78-79 0.6743653118923092 2315 80-81 0.7630260653493259 2315 82-83 0.7535076253741444 2315 84-85 0.7082987685956788 2315 86-87 0.7777505597044332 2315 88-89 0.8552215907016745 2315 90-91 0.9014261721634718 2315 92-93 0.9759846206021408 2315 94-95 1.0418895507109802 2315 96-97 1.0899292904744655 2315 98-99 0.9327637289324713 2315 100 0.8846221753765349 2316 1 0.5091220559279108 2316 2 0.532025518342337 2316 3 0.49544323292645487 2316 4 0.30848641265988874 2316 5 0.4117030511003392 2316 6 0.4964934622703012 2316 7 0.5258710505581448 2316 8 0.5384686047010661 2316 9 0.547900689393849 2316 10-11 0.43513159687935143 2316 12-13 0.62880037007276 2316 14-15 0.7366784873275591 2316 16-17 0.7989850881591778 2316 18-19 0.8900285105703531 2316 20-21 0.8891984095113727 2316 22-23 0.94655526096156 2316 24-25 0.9697802084303078 2316 26-27 0.9843610269026186 2316 28-29 0.9231289102678701 2316 30-31 0.7764665947205671 2316 32-33 0.7944533855869338 2316 34-35 0.7092500173309944 2316 36-37 0.6250909661315447 2316 38-39 0.5902626848745953 2316 40-41 0.6976515228413476 2316 42-43 0.911309338764184 2316 44-45 0.8280179503031135 2316 46-47 0.8493313425204931 2316 48-49 0.5582048812767368 2316 50-51 0.4897525085689196 2316 52-53 0.7327645656272423 2316 54-55 0.8556336433148672 2316 56-57 0.8879187980659253 2316 58-59 0.8534166439753577 2316 60-61 0.9029273762222161 2316 62-63 0.9644553071707378 2316 64-65 0.8799406376960945 2316 66-67 0.8976968384019557 2316 68-69 0.9007796896943887 2316 70-71 0.7421888079920862 2316 72-73 0.7167262778352423 2316 74-75 0.88084941644113 2316 76-77 0.6594962886841564 2316 78-79 0.5490556060816374 2316 80-81 0.6242569912137732 2316 82-83 0.5603423600203179 2316 84-85 0.6874079001911362 2316 86-87 0.7275357567742446 2316 88-89 0.7182610929461433 2316 90-91 0.8011258702782733 2316 92-93 0.6812924648193999 2316 94-95 0.6226208114703162 2316 96-97 0.7709690095849666 2316 98-99 1.0044235601607987 2316 100 0.3896719873939851 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 9.0 13 90.0 14 376.0 15 1055.0 16 2429.0 17 4766.0 18 7407.0 19 10193.0 20 13243.0 21 17149.0 22 20287.0 23 20464.0 24 20681.0 25 22460.0 26 25919.0 27 31125.0 28 38225.0 29 45750.0 30 56962.0 31 69676.0 32 87122.0 33 112991.0 34 156765.0 35 219271.0 36 326608.0 37 497870.0 38 429026.0 39 60464.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.477134721677466 19.679493011627773 13.19321618354386 23.6501560831509 2 16.25490016924891 21.457367983675528 39.185821379313346 23.101910467762217 3 18.536497287231494 26.683809101153415 31.038727108976282 23.740966502638805 4 12.856492606224332 17.725305415893896 38.17600763093697 31.242194346944796 5 12.784318844363224 37.46795457512074 34.824217900187094 14.92350868032894 6 29.167342864937133 37.43617812302443 18.825881954708276 14.570597057330156 7 26.022031972857484 33.34595087772424 22.919551842226742 17.712465307191522 8 25.590252956540237 34.90773279776747 20.941976932438035 18.560037313254256 9 26.704682410742336 15.927584445509947 20.84016070687355 36.527572436874166 10-11 24.543199198042444 25.937270490119978 29.07807512782939 20.441455184008188 12-13 25.20312628147019 24.33614813528888 28.464437319152946 21.996288264087987 14-15 22.81846606872598 25.78306496051472 26.47957471071579 24.918894260043505 16-17 21.8735621706793 28.256039617357654 27.311268143978396 22.559130067984647 18-19 21.466504814239535 27.50980033849782 29.537458829876567 21.486236017386084 20-21 22.250601997616158 26.964380928471872 29.320987587177893 21.46402948673408 22-23 22.32543215033132 26.690596461000958 29.11209585840523 21.87187553026249 24-25 21.99298899619603 26.63557094605913 29.341599130172597 22.02984092757225 26-27 21.962069100544294 26.799259481637144 29.176358233372056 22.062313184446502 28-29 21.672663670340285 27.389889356369952 29.177112475362975 21.76033449792679 30-31 22.42353388552153 26.838356383716988 29.238512842356606 21.499596888404874 32-33 22.296071534716262 27.02503358248411 28.869578990745726 21.809315892053906 34-35 22.4587854252774 26.9123203645095 29.130101299783608 21.498792910429493 36-37 22.135962386881893 26.742703275305967 29.160045941413813 21.961288396398327 38-39 22.054794013838954 26.739611916532834 29.250802949693718 21.954791119934498 40-41 21.938690046541335 26.27344742203989 29.636044756605394 22.151817774813377 42-43 21.692596126527235 26.648388344513087 29.145178434451484 22.513837094508194 44-45 21.319903306241645 26.910427300518 29.372349945076188 22.39731944816417 46-47 21.387404852120316 26.465040735313476 29.147055079974805 23.000499332591406 48-49 21.520739893729036 26.56739225401997 29.09150162991738 22.82036622233361 50-51 21.3065092011873 26.73527824173701 28.91596278666208 23.04224977041361 52-53 21.094900103436558 27.00403433031901 28.683844880847786 23.217220685396647 54-55 20.934741697216268 26.746679430417615 28.906284334308747 23.41229453805737 56-57 21.25905060507056 26.559449547431672 28.48012924183547 23.70137060566229 58-59 20.85296457065679 26.68694478480625 28.629305963602945 23.830784680934013 60-61 21.277809841780282 26.778897685278753 28.255823894877835 23.687468578063132 62-63 21.2008398138417 26.616707996744644 28.120710948406185 24.06174124100747 64-65 20.81536996026481 26.427753623535278 28.100887612202634 24.65598880399728 66-67 20.966274268778154 26.490241778589912 27.84039405050252 24.703089902129413 68-69 20.89497790840395 26.601072071995596 27.779580743972776 24.724369275627677 70-71 21.029338692690366 26.393571035095476 27.74346561743062 24.833624654783538 72-73 21.182101339387536 26.656388685283105 27.370544442838813 24.79096553249055 74-75 21.3460353769279 26.86099670524152 27.179701102017397 24.613266815813187 76-77 21.34172653181929 26.750208411898075 27.101924570451708 24.80614048583093 78-79 21.258814289102155 26.742982835932928 27.164915593867146 24.833287281097768 80-81 21.201108014921175 26.98716402175566 26.959165608114883 24.85256235520828 82-83 21.467348020565954 26.582898783085223 27.16324205384506 24.78651114250376 84-85 21.61896161722479 26.708820618697608 26.78858839760788 24.883629366469716 86-87 21.283592458730524 26.902261327422572 26.931339206078146 24.882807007768758 88-89 21.39313586499764 26.42760107469374 26.95924491946436 25.220018140844264 90-91 21.631507407969995 26.115003832271427 26.94635211719395 25.307136642564632 92-93 21.695092875049802 26.132310089927653 26.91638906315042 25.25620797187212 94-95 21.319509047413092 25.886988485901735 27.24349128414699 25.550011182538185 96-97 21.35159865431439 25.858218306684744 27.255985707746746 25.53419733125412 98-99 21.30946858297206 26.044260527247992 27.05042095066178 25.595849939118164 100 21.244705044774847 25.976286607755984 27.214568513913758 25.564439833555415 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1683.0 1 1157.5 2 1296.5 3 1958.0 4 1381.5 5 815.0 6 1067.5 7 1726.5 8 2565.0 9 3031.0 10 2518.5 11 1891.5 12 1877.5 13 2056.0 14 2295.5 15 2553.0 16 2823.0 17 3113.5 18 3351.5 19 3604.5 20 3971.0 21 4438.0 22 5038.0 23 5785.5 24 6831.5 25 8034.5 26 9744.5 27 12026.5 28 14472.5 29 17807.0 30 22281.5 31 26775.5 32 31695.0 33 36646.5 34 41478.0 35 46222.0 36 51122.5 37 57255.0 38 61596.5 39 63617.0 40 65036.5 41 67300.5 42 70567.0 43 74549.5 44 79452.0 45 85244.5 46 90243.0 47 94136.5 48 98104.5 49 99780.5 50 99434.5 51 98963.5 52 96394.0 53 91229.5 54 85429.5 55 77393.5 56 68770.0 57 59655.0 58 50249.0 59 42434.5 60 33954.0 61 25914.0 62 20046.5 63 15364.0 64 11101.0 65 7723.0 66 5524.0 67 4207.0 68 3128.5 69 2200.0 70 1626.5 71 1242.0 72 932.5 73 705.0 74 518.5 75 366.5 76 260.0 77 153.5 78 97.5 79 72.0 80 46.0 81 25.5 82 15.5 83 11.0 84 10.5 85 5.5 86 1.5 87 1.5 88 2.0 89 2.0 90 0.5 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05295010855424884 2 0.0 3 0.0 4 0.016359277581263404 5 0.00391578452743007 6 4.350871697144523E-5 7 1.3052615091433566E-4 8 4.785958866858975E-4 9 0.00191438354674359 10-11 6.091220376002333E-4 12-13 1.9578922637150355E-4 14-15 6.526307545716783E-5 16-17 1.5228050940005832E-4 18-19 0.0 20-21 2.1754358485722615E-5 22-23 0.0 24-25 1.3052615091433566E-4 26-27 0.0 28-29 8.701743394289045E-4 30-31 8.266656224574594E-4 32-33 0.013792263279948137 34-35 0.022080673863008455 36-37 0.029629436257554202 38-39 0.01986172929746475 40-41 1.3052615091433566E-4 42-43 0.0016968399618863639 44-45 0.008984550054603439 46-47 0.014031561223291087 48-49 0.004959993734744757 50-51 0.010246302846775352 52-53 0.015793664260634617 54-55 0.009354374148860724 56-57 0.0027410491692010496 58-59 0.007918586488803031 60-61 0.0015445594524863058 62-63 0.0010224548488289627 64-65 0.018578222146807113 66-67 0.04435713695238841 68-69 0.054364141855820815 70-71 0.04783783431010403 72-73 0.0401150370476725 74-75 0.028258911672953677 76-77 0.028563472691753793 78-79 0.011964897167147437 80-81 0.0019796466222007578 82-83 0.026627334786524477 84-85 0.04770730815918969 86-87 0.04833818455527565 88-89 0.059324135590565576 90-91 0.034676447426241847 92-93 0.02603996710740997 94-95 0.007135429583317017 96-97 0.0035677147916585083 98-99 0.021580323617836836 100 0.019491905203207465 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2298390.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.47430646254105 #Duplication Level Percentage of deduplicated Percentage of total 1 81.47942684854613 55.792472444195425 2 11.723477654401645 16.055140034285 3 3.1196904132792844 6.408559122814111 4 1.295180373079316 3.5474631116200537 5 0.6735837257619034 2.306158923300039 6 0.4153281900360397 1.7063585860236166 7 0.2772921867880649 1.329117312445589 8 0.20444116746465452 1.1199173723627542 9 0.14484084383986337 0.8926088696445471 >10 0.6375223938828462 7.182632278628552 >50 0.019813513255402176 0.930157160349419 >100 0.00844302946799975 0.9913306276716626 >500 4.112829414779054E-4 0.19924837280633106 >1k 4.112829414778945E-4 0.7182328859086954 >5k 1.3709431382596482E-4 0.8206028979441721 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9181 0.3994535305148386 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8302 0.36120936829693834 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4701 0.20453447848276401 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2959 0.1287422935185064 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2481 0.10794512680615562 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 4.350871697144523E-5 0.0 5 0.0 0.0 0.0 4.350871697144523E-5 0.0 6 0.0 0.0 0.0 4.350871697144523E-5 0.0 7 0.0 0.0 0.0 4.350871697144523E-5 0.0 8 0.0 0.0 0.0 4.350871697144523E-5 0.0 9 0.0 0.0 0.0 8.701743394289046E-5 0.0 10-11 0.0 0.0 0.0 1.305261509143357E-4 0.0 12-13 0.0 0.0 0.0 1.305261509143357E-4 0.0 14-15 0.0 0.0 0.0 1.522805094000583E-4 0.0 16-17 0.0 0.0 0.0 2.3929794334294876E-4 0.0 18-19 0.0 0.0 0.0 3.045610188001166E-4 4.350871697144523E-5 20-21 0.0 0.0 0.0 0.0011529809997432986 8.701743394289046E-5 22-23 4.350871697144523E-5 0.0 0.0 0.004590169640487472 8.701743394289046E-5 24-25 4.350871697144523E-5 0.0 0.0 0.013705245846005246 1.305261509143357E-4 26-27 4.350871697144523E-5 0.0 0.0 0.028541718333268074 1.305261509143357E-4 28-29 4.350871697144523E-5 0.0 0.0 0.06593746057022525 1.305261509143357E-4 30-31 4.350871697144523E-5 0.0 0.0 0.16126505945466174 1.7403486788578092E-4 32-33 4.350871697144523E-5 0.0 0.0 0.3715644429361422 1.7403486788578092E-4 34-35 4.350871697144523E-5 4.350871697144523E-5 0.0 0.6844791353947763 1.7403486788578092E-4 36-37 4.350871697144523E-5 4.350871697144523E-5 0.0 1.1253964731834023 1.7403486788578092E-4 38-39 4.350871697144523E-5 6.526307545716785E-5 0.0 1.7275353617097187 1.7403486788578092E-4 40-41 4.350871697144523E-5 8.701743394289046E-5 0.0 2.3629149100022193 1.7403486788578092E-4 42-43 4.350871697144523E-5 8.701743394289046E-5 0.0 2.9641836241891064 1.7403486788578092E-4 44-45 4.350871697144523E-5 8.701743394289046E-5 0.0 3.681337806029438 1.9578922637150355E-4 46-47 4.350871697144523E-5 8.701743394289046E-5 0.0 4.492840640622349 2.610523018286714E-4 48-49 4.350871697144523E-5 1.0877179242861308E-4 0.0 5.305213649554688 2.610523018286714E-4 50-51 4.350871697144523E-5 1.305261509143357E-4 0.0 6.109428774054882 3.045610188001166E-4 52-53 4.350871697144523E-5 1.305261509143357E-4 0.0 6.87146219745126 3.045610188001166E-4 54-55 4.350871697144523E-5 1.305261509143357E-4 0.0 7.704958688473235 3.045610188001166E-4 56-57 4.350871697144523E-5 1.305261509143357E-4 0.0 8.620425602269414 3.263153772858392E-4 58-59 4.350871697144523E-5 1.305261509143357E-4 0.0 9.602069274579161 3.4806973577156185E-4 60-61 4.350871697144523E-5 1.305261509143357E-4 0.0 10.561545255591957 3.4806973577156185E-4 62-63 4.350871697144523E-5 1.305261509143357E-4 0.0 11.503095645212518 3.4806973577156185E-4 64-65 4.350871697144523E-5 1.305261509143357E-4 0.0 12.43278990945836 3.4806973577156185E-4 66-67 4.350871697144523E-5 1.305261509143357E-4 0.0 13.42702935533134 3.698240942572844E-4 68-69 4.350871697144523E-5 1.305261509143357E-4 0.0 14.482006970096458 3.9157845274300705E-4 70-71 4.350871697144523E-5 1.305261509143357E-4 0.0 15.573205591740304 3.9157845274300705E-4 72-73 4.350871697144523E-5 1.305261509143357E-4 0.0 16.59646535183324 4.785958866858975E-4 74-75 4.350871697144523E-5 1.305261509143357E-4 0.0 17.58872515108402 4.785958866858975E-4 76-77 4.350871697144523E-5 1.305261509143357E-4 0.0 18.62808313645639 4.785958866858975E-4 78-79 4.350871697144523E-5 1.305261509143357E-4 0.0 19.73812103254887 4.785958866858975E-4 80-81 4.350871697144523E-5 1.305261509143357E-4 0.0 20.82686141168383 5.003502451716201E-4 82-83 4.350871697144523E-5 1.305261509143357E-4 0.0 21.898241812747184 5.221046036573428E-4 84-85 4.350871697144523E-5 1.305261509143357E-4 0.0 22.938752779119298 5.221046036573428E-4 86-87 4.350871697144523E-5 1.305261509143357E-4 0.0 23.95713956291143 5.438589621430655E-4 88 4.350871697144523E-5 1.305261509143357E-4 0.0 24.732269110116214 5.65613320628788E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4450 0.0 59.067097 1 TACCTGG 2130 0.0 44.131203 2 GTATCAA 7705 0.0 38.141808 1 ACCTGGG 2445 0.0 37.099987 3 ATCAACG 9100 0.0 31.55696 3 CAACGCA 9285 0.0 30.972078 5 TATCAAC 9605 0.0 30.387121 2 TCAACGC 9605 0.0 30.044594 4 GTGGTAT 1635 0.0 29.909517 1 TATCACG 160 2.1354936E-9 29.374832 2 AACGCAG 10005 0.0 28.976143 6 CCTGGGG 2420 0.0 27.966782 4 GTACATG 7255 0.0 26.83223 1 GTACCTG 3615 0.0 26.664867 1 ACGCAGA 10965 0.0 26.26784 7 CGCAGAG 11135 0.0 25.824606 8 TACATGG 7410 0.0 25.75154 2 TGGTATC 1880 0.0 25.74985 2 ACATGGG 7275 0.0 25.001917 3 TGGGGGG 3995 0.0 23.87546 6 >>END_MODULE