FastQCFastQC Report
Mon 6 Jun 2016
ERR1211462_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211462_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3798693
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161010.4238563105784016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137270.3613611313154288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77160.20312249502657886No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40310.10611544549664845No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63150.054.9091841
GTATCAA115350.042.0279581
TACCTGG30600.039.676622
GTGGTAT23850.035.9520381
TATCAAC143100.033.9372332
ATCAACG141450.033.8303263
CAACGCA141900.033.6567885
ACCTGGG37650.032.336743
TCAACGC148200.032.2260364
AACGCAG151500.032.021796
ACGCAGA168050.028.3367147
GTACATG117400.028.0510831
TGGTATC29800.027.6349162
CGCAGAG171100.027.6110638
TACATGG120250.027.0022752
GTACTAG13150.026.153971
ACATGGG124250.025.2721063
GCAGAGT183350.024.9958529
CATGGGG91400.023.8120174
ATGGGGG71750.023.5853315
Produced by FastQC (version 0.11.5)